Restriction map for pF1KSDA1281
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AclI        AflII       Alw44I      ApaLI       
AscI        Asp700I     AspI        AviII       AvrII       
BbsI        BcgI        BciVI       BfrI        BglI        
BlnI        BpuAI       BsaBI       BsiEI       Bsp1407I    
BspDI       BspHI       BsrGI       BssHII      BssSI       
BstBI       ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco52I      FseI        FspI        
HindIII     MamI        MfeI        MluI        MunI        
NaeI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    SspBI       SwaI        
Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       1712  GGTACC               G^GTACC
AccIII       3477  TCCGGA               T^CCGGA
AflIII       1060  ACATGT               A^C[GA][CT]GT
AgeI         1014  ACCGGT               A^CCGGT
AlwNI        3334  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1707  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       453  AGCGCT               AGC^GCT
AseI         2112  ATTAAT               AT^TAAT
AsnI         2112  ATTAAT               AT^TAAT
Asp718I      1712  GGTACC               G^GTACC
BamHI          36  GGATCC               G^GATCC
BbrPI        1182  CACGTG               CAC^GTG
BclI         3197  TGATCA               T^GATCA
BglII        1781  AGATCT               A^GATCT
BsaI          298  GAGACC               GGTCTC(1/5)
BseAI        3477  TCCGGA               T^CCGGA
BsiWI        1199  CGTACG               C^GTACG
BsmBI        2476  GAGACG               CGTCTC(1/5)
BspEI        3477  TCCGGA               T^CCGGA
BspLU11I     1060  ACATGT               A^CATGT
BsrBI        4318  GAGCGG               CCGCTC(-3/-3)
Bst1107I     3736  GTATAC               GTA^TAC
BstEII       4411  GGTTACC              G^GT[ACGT]ACC
BstPI        4411  GGTTACC              G^GT[ACGT]ACC
BstZ17I      3736  GTATAC               GTA^TAC
Bsu36I       1707  CCTGAGG              CC^T[ACGT]AGG
DsaI          533  CCGCGG               C^C[GA][CT]GG
Eco47III      453  AGCGCT               AGC^GCT
Eco81I       1707  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4411  GGTTACC              G^GT[ACGT]ACC
EcoRV        1465  GATATC               GAT^ATC
EcoT22I      3248  ATGCAT               ATGCA^T
FbaI         3197  TGATCA               T^GATCA
HpaI         3861  GTTAAC               GTT^AAC
KpnI         1712  GGTACC               GGTAC^C
KspI          533  CCGCGG               CCGC^GG
MroI         3477  TCCGGA               T^CCGGA
NdeI         3251  CATATG               CA^TATG
NsiI         3248  ATGCAT               ATGCA^T
PflMI        3333  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1014  ACCGGT               A^CCGGT
PmaCI        1182  CACGTG               CAC^GTG
PmlI         1182  CACGTG               CAC^GTG
PpuMI        1903  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2112  ATTAAT               AT^TAAT
SacI         3394  GAGCTC               GAGCT^C
SacII         533  CCGCGG               CCGC^GG
SapI         1640  GCTCTTC              GCTCTTC(1/4)
SpeI          169  ACTAGT               A^CTAGT
SspI         1003  AATATT               AAT^ATT
StuI          266  AGGCCT               AGG^CCT
Van91I       3333  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccI         3679  GTAGAC               GT^[AC][GT]AC
             3736  GTATAC              
AccII         534  CGCG                 CG^CG
              738  CGCG                
AcyI          422  GGCGCC               G[GA]^CG[CT]C
              598  GGCGCC              
AhdI         2854  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3268  GACCAGAAGTC         
ApaI         1099  GGGCCC               GGGCC^C
             1238  GGGCCC              
AspEI        2854  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3268  GACCAGAAGTC         
AspHI        3069  GAGCAC               G[AT]GC[AT]^C
             3394  GAGCTC              
BbeI          422  GGCGCC               GGCGC^C
              598  GGCGCC              
BlpI         3598  GCTAAGC              GC^T[ACGT]AGC
             4315  GCTGAGC             
Bpu1102I     3598  GCTAAGC              GC^T[ACGT]AGC
             4315  GCTGAGC             
BsaAI        1182  CACGTG               [CT]AC^GT[GA]
             1201  TACGTG              
BsaHI         422  GGCGCC               G[GA]^CG[CT]C
              598  GGCGCC              
BsaWI        1014  ACCGGT               [AT]^CCGG[AT]
             3477  TCCGGA              
BsgI         1221  CTGCAC               GTGCAG(16/14)
             4378  CTGCAC              
BsiHKAI      3069  GAGCAC               G[AT]GC[AT]^C
             3394  GAGCTC              
BsrFI        1014  ACCGGT               [GA]^CCGG[CT]
             1872  ACCGGC              
BstAPI        642  GCACGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1312  GCAAGGTCTGC         
BstUI         534  CGCG                 CG^CG
              738  CGCG                
BstYI          36  GGATCC               [GA]^GATC[CT]
             1781  AGATCT              
CelII        3598  GCTAAGC              GC^T[ACGT]AGC
             4315  GCTGAGC             
Cfr10I       1014  ACCGGT               [GA]^CCGG[CT]
             1872  ACCGGC              
DraI         2559  TTTAAA               TTT^AAA
             3697  TTTAAA              
Eam1105I     2854  GACAGCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3268  GACCAGAAGTC         
Hin1I         422  GGCGCC               G[GA]^CG[CT]C
              598  GGCGCC              
KasI          422  GGCGCC               G^GCGCC
              598  GGCGCC              
MflI           36  GGATCC               [GA]^GATC[CT]
             1781  AGATCT              
MvnI          534  CGCG                 CG^CG
              738  CGCG                
NarI          422  GGCGCC               GG^CGCC
              598  GGCGCC              
PaeR7I       2703  CTCGAG               C^TCGAG
             3690  CTCGAG              
ScaI         3320  AGTACT               AGT^ACT
             4031  AGTACT              
SfoI          422  GGCGCC               GGC^GCC
              598  GGCGCC              
SmaI         1332  CCCGGG               CCC^GGG
             4425  CCCGGG              
SphI         4038  GCATGC               GCATG^C
             4263  GCATGC              
XhoI         2703  CTCGAG               C^TCGAG
             3690  CTCGAG              
XhoII          36  GGATCC               [GA]^GATC[CT]
             1781  AGATCT              
XmaCI        1332  CCCGGG               C^CCGGG
             4425  CCCGGG              
XmaI         1332  CCCGGG               C^CCGGG
             4425  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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