Restriction map for pF1KSDA1269
 (Linear) (Six-base) MAPSORT of: KIAA1269.seq  Check: 8989  from: 1  to: 1590



KIAA1269.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     529    1110    1590 
   Size:        529     581     480

  Fragments arranged by size: 

                 581     529     480
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     436     739    1018    1228    1590 
   Size:        260     176     303     279     210     362

  Fragments arranged by size: 

                 362     303     279     260     210     176
 
AflIII A'CryG_T

Cuts at:      0    1349    1590 
   Size:       1349     241
 
AleI CACnn'nnGTG

Cuts at:      0      64    1498    1590 
   Size:         64    1434      92

  Fragments arranged by size: 

                1434      92      64
 
AlwNI CAG_nnn'CTG

Cuts at:      0     768    1519    1590 
   Size:        768     751      71

  Fragments arranged by size: 

                 768     751      71
 
ApaI G_GGCC'C

Cuts at:      0    1453    1590 
   Size:       1453     137
 
ApaLI G'TGCA_C

Cuts at:      0      22     391    1590 
   Size:         22     369    1199

  Fragments arranged by size: 

                1199     369      22
 
ApoI r'AATT_y

Cuts at:      0     385    1590 
   Size:        385    1205
 
AvaI C'yCGr_G

Cuts at:      0     938    1155    1282    1525    1590 
   Size:        938     217     127     243      65

  Fragments arranged by size: 

                 938     243     217     127      65
 
BanI G'GyrC_C

Cuts at:      0    1248    1590 
   Size:       1248     342
 
BanII G_rGCy'C

Cuts at:      0     555    1453    1590 
   Size:        555     898     137

  Fragments arranged by size: 

                 898     555     137
 
BbeI G_GCGC'C

Cuts at:      0    1252    1590 
   Size:       1252     338
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     665    1590 
   Size:        100     565     925

  Fragments arranged by size: 

                 925     565     100
 
BbvCI CC'TCA_GC

Cuts at:      0    1018    1590 
   Size:       1018     572
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1282    1590 
   Size:       1282     308
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1111    1144    1590 
   Size:       1111      33     446

  Fragments arranged by size: 

                1111     446      33
 
Bme1580I G_kGCm'C

Cuts at:      0      26     395    1166    1190    1453    1511    1590 
   Size:         26     369     771      24     263      58      79

  Fragments arranged by size: 

                 771     369     263      79      58      26      24
 
BmgBI CAC'GTC

Cuts at:      0      15     578     618    1590 
   Size:         15     563      40     972

  Fragments arranged by size: 

                 972     563      40      15
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1125    1135    1590 
   Size:       1125      10     455

  Fragments arranged by size: 

                1125     455      10
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     322    1590 
   Size:        322    1268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     976    1473    1590 
   Size:        333     643     497     117

  Fragments arranged by size: 

                 643     497     333     117
 
Bpu10I CC'TnA_GC

Cuts at:      0     457     565    1018    1369    1590 
   Size:        457     108     453     351     221

  Fragments arranged by size: 

                 457     453     351     221     108
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196    1590 
   Size:        196    1394
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     632     643     769    1053    1590 
   Size:        632      11     126     284     537

  Fragments arranged by size: 

                 632     537     284     126      11
 
BsaAI yAC'GTr

Cuts at:      0    1087    1590 
   Size:       1087     503
 
BsaBI GATnn'nnATC

Cuts at:      0     974    1590 
   Size:        974     616
 
BsaHI Gr'CG_yC

Cuts at:      0     725    1042    1249    1590 
   Size:        725     317     207     341

  Fragments arranged by size: 

                 725     341     317     207
 
BsaWI w'CCGG_w

Cuts at:      0      54    1590 
   Size:         54    1536
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     542     572     949     979    1590 
   Size:        542      30     377      30     611

  Fragments arranged by size: 

                 611     542     377      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     192    1549    1590 
   Size:         46     146    1357      41

  Fragments arranged by size: 

                1357     146      46      41
 
BseYI C'CCAG_C

Cuts at:      0     206    1010    1172    1324    1590 
   Size:        206     804     162     152     266

  Fragments arranged by size: 

                 804     266     206     162     152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1405    1590 
   Size:        874     531     185

  Fragments arranged by size: 

                 874     531     185
 
BsiEI CG_ry'CG

Cuts at:      0     584    1106    1379    1590 
   Size:        584     522     273     211

  Fragments arranged by size: 

                 584     522     273     211
 
BsiHKAI G_wGCw'C

Cuts at:      0      26     395    1031    1127    1375    1541    1590 
   Size:         26     369     636      96     248     166      49

  Fragments arranged by size: 

                 636     369     248     166      96      49      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     395     555    1031    1127    1166    1190    1375 
   Size:         26     369     160     476      96      39      24     185

Cuts at:   1375    1453    1511    1541    1590 
   Size:         78      58      30      49

  Fragments arranged by size: 

                 476     369     185     160      96      78      58      49
                  39      30      26      24
 
BspEI T'CCGG_A

Cuts at:      0      54    1590 
   Size:         54    1536
 
BspHI T'CATG_A

Cuts at:      0    1031    1590 
   Size:       1031     559
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     529    1110    1590 
   Size:        529     581     480

  Fragments arranged by size: 

                 581     529     480
 
BsrBI CCG'CTC

Cuts at:      0     920    1584    1590 
   Size:        920     664       6

  Fragments arranged by size: 

                 920     664       6
 
BsrFI r'CCGG_y

Cuts at:      0     727    1044    1101    1590 
   Size:        727     317      57     489

  Fragments arranged by size: 

                 727     489     317      57
 
BssSI C'ACGA_G

Cuts at:      0    1579    1590 
   Size:       1579      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1519    1590 
   Size:       1519      71
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1227    1332    1557    1590 
   Size:       1227     105     225      33

  Fragments arranged by size: 

                1227     225     105      33
 
BstYI r'GATC_y

Cuts at:      0    1337    1590 
   Size:       1337     253
 
Bsu36I CC'TnA_GG

Cuts at:      0    1532    1590 
   Size:       1532      58
 
BtgI C'CryG_G

Cuts at:      0     185     677    1109    1565    1590 
   Size:        185     492     432     456      25

  Fragments arranged by size: 

                 492     456     432     185      25
 
BtsI GCAGTG_nn'

Cuts at:      0      59      71      81     970    1590 
   Size:         59      12      10     889     620

  Fragments arranged by size: 

                 889     620      59      12      10
 
EaeI y'GGCC_r

Cuts at:      0     398     581     680     893    1103    1376    1544    1590 
   Size:        398     183      99     213     210     273     168      46

  Fragments arranged by size: 

                 398     273     213     210     183     168      99      46
            
 
EagI C'GGCC_G

Cuts at:      0     581    1103    1376    1590 
   Size:        581     522     273     214

  Fragments arranged by size: 

                 581     522     273     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     236     421    1311    1590 
   Size:        236     185     890     279

  Fragments arranged by size: 

                 890     279     236     185
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     333     436     739     976    1018    1228    1473 
   Size:        260      73     103     303     237      42     210     245

Cuts at:   1473    1590 
   Size:        117

  Fragments arranged by size: 

                 303     260     245     237     210     117     103      73
                  42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1231    1590 
   Size:       1231     359
 
EcoO109I rG'GnC_Cy

Cuts at:      0     807     942    1450    1590 
   Size:        807     135     508     140

  Fragments arranged by size: 

                 807     508     140     135
 
HaeII r_GCGC'y

Cuts at:      0    1150    1252    1590 
   Size:       1150     102     338

  Fragments arranged by size: 

                1150     338     102
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1280    1312    1590 
   Size:       1280      32     278

  Fragments arranged by size: 

                1280     278      32
 
KasI G'GCGC_C

Cuts at:      0    1248    1590 
   Size:       1248     342
 
MfeI C'AATT_G

Cuts at:      0    1496    1590 
   Size:       1496      94
 
MluI A'CGCG_T

Cuts at:      0    1349    1590 
   Size:       1349     241
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     539    1590 
   Size:        539    1051
 
MscI TGG'CCA

Cuts at:      0     895    1590 
   Size:        895     695
 
MslI CAynn'nnrTG

Cuts at:      0      64     340     439    1498    1555    1590 
   Size:         64     276      99    1059      57      35

  Fragments arranged by size: 

                1059     276      99      64      57      35
 
MspA1I CmG'CkG

Cuts at:      0     172     181     187     679     798    1176    1278    1422 
   Size:        172       9       6     492     119     378     102     144

Cuts at:   1422    1428    1590 
   Size:          6     162

  Fragments arranged by size: 

                 492     378     172     162     144     119     102       9
                   6       6
 
NaeI GCC'GGC

Cuts at:      0     729    1590 
   Size:        729     861
 
NarI GG'CG_CC

Cuts at:      0    1249    1590 
   Size:       1249     341
 
NcoI C'CATG_G

Cuts at:      0    1109    1565    1590 
   Size:       1109     456      25

  Fragments arranged by size: 

                1109     456      25
 
NgoMIV G'CCGG_C

Cuts at:      0     727    1590 
   Size:        727     863
 
NspI r_CATG'y

Cuts at:      0     370     870    1590 
   Size:        370     500     720

  Fragments arranged by size: 

                 720     500     370
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1590 
   Size:        768     822
 
PfoI T'CCnGG_A

Cuts at:      0     749     803    1590 
   Size:        749      54     787

  Fragments arranged by size: 

                 787     749      54
 
PmlI CAC'GTG

Cuts at:      0    1087    1590 
   Size:       1087     503
 
PpuMI rG'GwC_Cy

Cuts at:      0     807     942    1590 
   Size:        807     135     648

  Fragments arranged by size: 

                 807     648     135
 
PshAI GACnn'nnGTC

Cuts at:      0    1045    1590 
   Size:       1045     545
 
PspOMI G'GGCC_C

Cuts at:      0    1449    1590 
   Size:       1449     141
 
PstI C_TGCA'G

Cuts at:      0      66     794     821    1427    1463    1590 
   Size:         66     728      27     606      36     127

  Fragments arranged by size: 

                 728     606     127      66      36      27
 
PvuII CAG'CTG

Cuts at:      0     181    1176    1428    1590 
   Size:        181     995     252     162

  Fragments arranged by size: 

                 995     252     181     162
 
SacII CC_GC'GG

Cuts at:      0     188     680    1590 
   Size:        188     492     910

  Fragments arranged by size: 

                 910     492     188
 
SexAI A'CCwGG_T

Cuts at:      0     875    1590 
   Size:        875     715
 
SfcI C'TryA_G

Cuts at:      0      62     790     817    1423    1459    1590 
   Size:         62     728      27     606      36     131

  Fragments arranged by size: 

                 728     606     131      62      36      27
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1111    1590 
   Size:       1111     479
 
SfoI GGC'GCC

Cuts at:      0    1250    1590 
   Size:       1250     340
 
SmaI CCC'GGG

Cuts at:      0     940    1157    1527    1590 
   Size:        940     217     370      63

  Fragments arranged by size: 

                 940     370     217      63
 
SmlI C'TyrA_G

Cuts at:      0     211    1590 
   Size:        211    1379
 
SphI G_CATG'C

Cuts at:      0     870    1590 
   Size:        870     720
 
SrfI GCCC'GGGC

Cuts at:      0    1157    1590 
   Size:       1157     433
 
StuI AGG'CCT

Cuts at:      0     788    1477    1590 
   Size:        788     689     113

  Fragments arranged by size: 

                 788     689     113
 
StyI C'CwwG_G

Cuts at:      0     989    1109    1191    1565    1590 
   Size:        989     120      82     374      25

  Fragments arranged by size: 

                 989     374     120      82      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1144    1590 
   Size:       1144     446
 
TatI w'GTAC_w

Cuts at:      0     587     834    1590 
   Size:        587     247     756

  Fragments arranged by size: 

                 756     587     247
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     542     574     949     981    1590 
   Size:        542      32     375      32     609

  Fragments arranged by size: 

                 609     542     375      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     505     742    1590 
   Size:        505     237     848

  Fragments arranged by size: 

                 848     505     237
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1480    1554    1590 
   Size:       1480      74      36

  Fragments arranged by size: 

                1480      74      36
 
XmaI C'CCGG_G

Cuts at:      0     938    1155    1525    1590 
   Size:        938     217     370      65

  Fragments arranged by size: 

                 938     370     217      65
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI     PstI    PvuII    SacII    SexAI     SfcI     SfiI     SfoI
     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI     BclI    BfrBI    BglII     BlpI     BmtI    BsiWI     BsmI
    BsmBI    BsrDI    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI     SgfI    SgrAI
    SnaBI     SpeI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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