Restriction map for pF1KSDA1229
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeII       
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

BalI         1724  TGGCCA               TGG^CCA
BsaBI        1522  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1478  GTGCAG               GTGCAG(16/14)
BspMI         653  ACCTGC               ACCTGC(4/8)
BssSI        1438  CACGAG               CACGAG(-5/-1)
BstF5I       1164  GGATG                GGATG(2/0)
Cac8I        1650  GCTGGC               GC[ACGT]^[ACGT]GC
EaeI         1724  TGGCCA               [CT]^GGCC[GA]
EcoNI         114  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       963  CCATGG               C^C[AT][AT]GG
FokI         1164  GGATG                GGATG(9/13)
HgaI         1621  GACGC                GACGC(5/10)
MaeIII        178  GTAAC                ^GT[ACGT]AC
MamI         1522  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1007  CAATTG               C^AATTG
MluNI        1724  TGGCCA               TGG^CCA
MscI         1724  TGGCCA               TGG^CCA
MslI         1015  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1007  CAATTG               C^AATTG
MwoI          838  GCTTCCAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          963  CCATGG               C^CATGG
NlaIV         650  GGAACC               GG[ACGT]^[ACGT]CC
NspI          392  GCATGT               [GA]CATG^[CT]
PflMI         842  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         1858  GAAGAGC              GCTCTTC(1/4)
SfaNI         733  GCATC                GCATC(5/9)
StyI          963  CCATGG               C^C[AT][AT]GG
Van91I        842  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1795  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI          570  GATCC                GGATC(4/5)
             1894  GATCC               
BbsI          958  GAAGAC               GAAGAC(2/6)
             1757  GAAGAC              
BglII         942  AGATCT               A^GATCT
             1827  AGATCT              
BpuAI         958  GAAGAC               GAAGAC(2/6)
             1757  GAAGAC              
BsaJI          82  CCACGG               C^C[ACGT][ACGT]GG
              963  CCATGG              
BsiYI         114  CCTGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              842  CCAATGCCTGG         
BslI          114  CCTGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              842  CCAATGCCTGG         
BsrI         1734  ACTGG                ACTGG(1/-1)
               92  CCAGT               
BstXI        1515  CCAGGTTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1727  CCAGACAACTGG        
Cfr13I        465  GGCCC                G^G[ACGT]CC
             1512  GGGCC               
DraI          723  TTTAAA               TTT^AAA
             1693  TTTAAA              
DsaI           82  CCACGG               C^C[GA][CT]GG
              963  CCATGG              
EarI         1558  GAAGAG               CTCTTC(1/4)
             1858  GAAGAG              
Ksp632I      1558  GAAGAG               CTCTTC(1/4)
             1858  GAAGAG              
PstI         1456  CTGCAG               CTGCA^G
             1706  CTGCAG              
Sau96I        465  GGCCC                G^G[ACGT]CC
             1512  GGGCC               
SspI          291  AATATT               AAT^ATT
             1821  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
ApoI        AseI        AsnI        Asp700I     BbvI        
BfaI        BfrI        BsmAI       BssKI       BstNI       
BstYI       DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      HindIII     HinfI       ItaI        MaeI        
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        NdeII       NlaIII      PleI        
PshBI       PvuII       RsaI        Sau3AI      ScrFI       
SfcI        SmlI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       XmnI        
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