Restriction map for pF1KSDA1229
 (Linear) (Six-base) MAPSORT of: KIAA1229.seq  Check: 2353  from: 1  to: 1908



KIAA1229.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     967    1405    1492    1603    1793    1908 
   Size:        136     831     438      87     111     190     115

  Fragments arranged by size: 

                 831     438     190     136     115     111      87
 
AflII C'TTAA_G

Cuts at:      0     160     439    1672    1687    1908 
   Size:        160     279    1233      15     221

  Fragments arranged by size: 

                1233     279     221     160      15
 
ApoI r'AATT_y

Cuts at:      0     264     575     972     986    1302    1908 
   Size:        264     311     397      14     316     606

  Fragments arranged by size: 

                 606     397     316     311     264      14
 
AseI AT'TA_AT

Cuts at:      0     233     295     929    1908 
   Size:        233      62     634     979

  Fragments arranged by size: 

                 979     634     233      62
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1246    1279    1908 
   Size:       1246      33     629

  Fragments arranged by size: 

                1246     629      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     965    1764    1908 
   Size:        965     799     144

  Fragments arranged by size: 

                 965     799     144
 
BglII A'GATC_T

Cuts at:      0     942    1827    1908 
   Size:        942     885      81

  Fragments arranged by size: 

                 942     885      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1057    1660    1908 
   Size:       1057     603     248

  Fragments arranged by size: 

                1057     603     248
 
BsaBI GATnn'nnATC

Cuts at:      0    1526    1908 
   Size:       1526     382
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1499    1908 
   Size:       1499     409
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     662    1908 
   Size:        662    1246
 
BssSI C'ACGA_G

Cuts at:      0    1438    1908 
   Size:       1438     470
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1522    1734    1908 
   Size:       1522     212     174

  Fragments arranged by size: 

                1522     212     174
 
BstYI r'GATC_y

Cuts at:      0     569     942    1827    1908 
   Size:        569     373     885      81

  Fragments arranged by size: 

                 885     569     373      81
 
BtgI C'CryG_G

Cuts at:      0      82     963    1908 
   Size:         82     881     945

  Fragments arranged by size: 

                 945     881      82
 
BtsI GCAGTG_nn'

Cuts at:      0    1465    1908 
   Size:       1465     443
 
DraI TTT'AAA

Cuts at:      0     725    1695    1908 
   Size:        725     970     213

  Fragments arranged by size: 

                 970     725     213
 
EaeI y'GGCC_r

Cuts at:      0    1724    1908 
   Size:       1724     184
 
EarI CTCTTCn'nnn_

Cuts at:      0    1553    1853    1908 
   Size:       1553     300      55

  Fragments arranged by size: 

                1553     300      55
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1438    1908 
   Size:       1438     470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     967    1405    1492    1603    1793    1908 
   Size:        136     831     438      87     111     190     115

  Fragments arranged by size: 

                 831     438     190     136     115     111      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118    1908 
   Size:        118    1790
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     175    1006    1687    1908 
   Size:        175     831     681     221

  Fragments arranged by size: 

                 831     681     221     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     554     586    1362    1394    1774    1806 
   Size:        370      32     152      32     776      32     380      32

Cuts at:   1806    1908 
   Size:        102

  Fragments arranged by size: 

                 776     380     370     152     102      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     784    1054    1546    1908 
   Size:        784     270     492     362

  Fragments arranged by size: 

                 784     492     362     270
 
MfeI C'AATT_G

Cuts at:      0    1007    1908 
   Size:       1007     901
 
MscI TGG'CCA

Cuts at:      0    1726    1908 
   Size:       1726     182
 
MslI CAynn'nnrTG

Cuts at:      0    1019    1908 
   Size:       1019     889
 
MspA1I CmG'CkG

Cuts at:      0     110    1276    1581    1908 
   Size:        110    1166     305     327

  Fragments arranged by size: 

                1166     327     305     110
 
NcoI C'CATG_G

Cuts at:      0     963    1908 
   Size:        963     945
 
NspI r_CATG'y

Cuts at:      0     396    1908 
   Size:        396    1512
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     848    1908 
   Size:        848    1060
 
PstI C_TGCA'G

Cuts at:      0    1460    1710    1908 
   Size:       1460     250     198

  Fragments arranged by size: 

                1460     250     198
 
PvuII CAG'CTG

Cuts at:      0     110    1276    1581    1908 
   Size:        110    1166     305     327

  Fragments arranged by size: 

                1166     327     305     110
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1853    1908 
   Size:       1853      55
 
SfcI C'TryA_G

Cuts at:      0     188     565     683     716    1456    1706    1908 
   Size:        188     377     118      33     740     250     202

  Fragments arranged by size: 

                 740     377     250     202     188     118      33
 
SmlI C'TyrA_G

Cuts at:      0     160     439    1036    1639    1672    1687    1908 
   Size:        160     279     597     603      33      15     221

  Fragments arranged by size: 

                 603     597     279     221     160      33      15
 
SspI AAT'ATT

Cuts at:      0     293    1823    1908 
   Size:        293    1530      85

  Fragments arranged by size: 

                1530     293      85
 
StyI C'CwwG_G

Cuts at:      0     963    1908 
   Size:        963     945
 
TatI w'GTAC_w

Cuts at:      0     552     897    1908 
   Size:        552     345    1011

  Fragments arranged by size: 

                1011     552     345
 
XbaI T'CTAG_A

Cuts at:      0    1795    1908 
   Size:       1795     113
 
XmnI GAAnn'nnTTC

Cuts at:      0      27     344     972     986    1595    1908 
   Size:         27     317     628      14     609     313

  Fragments arranged by size: 

                 628     609     317     313      27      14
 

 Enzymes that do cut:

     AcuI    AflII     ApoI     AseI     BaeI     BbsI    BglII    BpuEI
    BsaBI     BsgI    BspMI    BssSI    BstXI    BstYI     BtgI     BtsI
     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI     FalI    Hin4I
  HindIII     MfeI     MscI     MslI   MspA1I     NcoI     NspI    PflMI
     PstI    PvuII     SapI     SfcI     SmlI     SspI     StyI     TatI
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     ZraI
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