Restriction map for pF1KSDA1186
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       CfoI        ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MslI        MunI        
MvnI        NarI        NciI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        167  TCCGGA               T^CCGGA
AocI          906  CCTCAGG              CC^T[ACGT]AGG
BalI           47  TGGCCA               TGG^CCA
BanII          24  GAGCCC               G[GA]GC[CT]^C
BbsI          960  GAAGAC               GAAGAC(2/6)
BbvCI         360  GCTGAGG              CCTCAGC(-5/-2)
BpmI          227  CTCCAG               CTGGAG(16/14)
BpuAI         960  GAAGAC               GAAGAC(2/6)
BsaWI         167  TCCGGA               [AT]^CCGG[AT]
BseAI         167  TCCGGA               T^CCGGA
BseRI         861  CTCCTC               GAGGAG(10/8)
BsgI          844  GTGCAG               GTGCAG(16/14)
BspEI         167  TCCGGA               T^CCGGA
BsrFI          81  GCCGGC               [GA]^CCGG[CT]
BsrI          289  CCAGT                ACTGG(1/-1)
BstXI         416  CCACAAAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         884  AGATCC               [GA]^GATC[CT]
Bsu36I        906  CCTCAGG              CC^T[ACGT]AGG
Cfr10I         81  GCCGGC               [GA]^CCGG[CT]
DsaI          855  CCGTGG               C^C[GA][CT]GG
EaeI           47  TGGCCA               [CT]^GGCC[GA]
Eco81I        906  CCTCAGG              CC^T[ACGT]AGG
EcoNI         624  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       323  CCAAGG               C^C[AT][AT]GG
HgaI          561  GCGTC                GACGC(5/10)
HindIII       135  AAGCTT               A^AGCTT
MaeIII        785  GTGAC                ^GT[ACGT]AC
MflI          884  AGATCC               [GA]^GATC[CT]
MluNI          47  TGGCCA               TGG^CCA
MroI          167  TCCGGA               T^CCGGA
MscI           47  TGGCCA               TGG^CCA
MseI          575  TTAA                 T^TAA
NaeI           81  GCCGGC               GCC^GGC
NgoMIV         81  GCCGGC               G^CCGGC
PflMI         867  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           11  GAGTC                GAGTC(4/5)
PvuII        1186  CAGCTG               CAG^CTG
SmlI          478  CTCAAG               C^T[CT][GA]AG
StuI          506  AGGCCT               AGG^CCT
StyI          323  CCAAGG               C^C[AT][AT]GG
TaqI          140  TCGA                 T^CGA
TfiI           93  GAATC                G^A[AT]TC
Tru9I         575  TTAA                 T^TAA
Tsp45I        785  GTGAC                ^GT[GC]AC
TthHB8I       140  TCGA                 T^CGA
Van91I        867  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         884  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          297  GTAC                 GT^AC
             1023  GTAC                
AlwNI         244  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              352  CAGGACCTG           
BsaI          439  GAGACC               GGTCTC(1/5)
              812  GAGACC              
BsmAI         439  GAGAC                GTCTC(1/5)
              812  GAGAC               
BsmFI        1051  GGGAC                GGGAC(10/14)
             1039  GTCCC               
BsrBI           3  GAGCGG               CCGCTC(-3/-3)
              932  GAGCGG              
DraIII        838  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              868  CACCAAGTG           
EarI          889  CTCTTC               CTCTTC(1/4)
             1171  GAAGAG              
FauI          375  GCGGG                CCCGC(4/6)
              984  GCGGG               
HapII          82  CCGG                 C^CGG
              168  CCGG                
HinfI          11  GAGTC                G^A[ACGT]TC
               93  GAATC               
HpaII          82  CCGG                 C^CGG
              168  CCGG                
Ksp632I       889  CTCTTC               CTCTTC(1/4)
             1171  GAAGAG              
MspA1I        982  CAGCGG               C[AC]G^C[GT]G
             1186  CAGCTG              
MspI           82  CCGG                 C^CGG
              168  CCGG                
NspI          174  GCATGT               [GA]CATG^[CT]
              527  GCATGT              
PstI          145  CTGCAG               CTGCA^G
             1062  CTGCAG              
RsaI          297  GTAC                 GT^AC
             1023  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanI        
BbvI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BspMI       BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PpuMI       Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        TseI        Tsp509I     TspRI       
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