Restriction map for pF1KSDA1186
 (Linear) (Six-base) MAPSORT of: KIAA1186.seq  Check: 9433  from: 1  to: 1209



KIAA1186.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1069    1209 
   Size:       1069     140
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     979    1064    1209 
   Size:        577     402      85     145

  Fragments arranged by size: 

                 577     402     145      85
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     419    1209 
   Size:        419     790
 
AlwNI CAG_nnn'CTG

Cuts at:      0     249     357    1209 
   Size:        249     108     852

  Fragments arranged by size: 

                 852     249     108
 
BanI G'GyrC_C

Cuts at:      0     708     727    1198    1209 
   Size:        708      19     471      11

  Fragments arranged by size: 

                 708     471      19      11
 
BanII G_rGCy'C

Cuts at:      0      28    1209 
   Size:         28    1181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     967    1209 
   Size:        967     242
 
BbvCI CC'TCA_GC

Cuts at:      0     361    1209 
   Size:        361     848
 
Bme1580I G_kGCm'C

Cuts at:      0     408     730    1209 
   Size:        408     322     479

  Fragments arranged by size: 

                 479     408     322
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     283    1209 
   Size:        283     926
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     538    1209 
   Size:        538     671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212    1209 
   Size:        212     997
 
Bpu10I CC'TnA_GC

Cuts at:      0     361    1209 
   Size:        361     848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1209 
   Size:        463     746
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     433     806    1209 
   Size:        433     373     403

  Fragments arranged by size: 

                 433     403     373
 
BsaWI w'CCGG_w

Cuts at:      0     167    1209 
   Size:        167    1042
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     923     953    1209 
   Size:        923      30     256

  Fragments arranged by size: 

                 923     256      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     852    1209 
   Size:        852     357
 
BseYI C'CCAG_C

Cuts at:      0    1188    1209 
   Size:       1188      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1209 
   Size:        865     344
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     408     730    1209 
   Size:         28     380     322     479

  Fragments arranged by size: 

                 479     380     322      28
 
BspEI T'CCGG_A

Cuts at:      0     167    1209 
   Size:        167    1042
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     152     365    1069    1209 
   Size:        152     213     704     140

  Fragments arranged by size: 

                 704     213     152     140
 
BsrBI CCG'CTC

Cuts at:      0       5     934    1209 
   Size:          5     929     275

  Fragments arranged by size: 

                 929     275       5
 
BsrFI r'CCGG_y

Cuts at:      0      81    1209 
   Size:         81    1128
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     423    1209 
   Size:        423     786
 
BstYI r'GATC_y

Cuts at:      0     884    1209 
   Size:        884     325
 
Bsu36I CC'TnA_GG

Cuts at:      0     907    1209 
   Size:        907     302
 
BtgI C'CryG_G

Cuts at:      0     855    1209 
   Size:        855     354
 
BtsI GCAGTG_nn'

Cuts at:      0    1071    1209 
   Size:       1071     138
 
DraIII CAC_nnn'GTG

Cuts at:      0     843     873    1209 
   Size:        843      30     336

  Fragments arranged by size: 

                 843     336      30
 
EaeI y'GGCC_r

Cuts at:      0      47    1209 
   Size:         47    1162
 
EarI CTCTTCn'nnn_

Cuts at:      0     895    1166    1209 
   Size:        895     271      43

  Fragments arranged by size: 

                 895     271      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     577     979    1064    1209 
   Size:        212     365     402      85     145

  Fragments arranged by size: 

                 402     365     212     145      85
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     628    1209 
   Size:        628     581
 
EcoO109I rG'GnC_Cy

Cuts at:      0     354    1052    1146    1209 
   Size:        354     698      94      63

  Fragments arranged by size: 

                 698     354      94      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1150    1182    1209 
   Size:       1150      32      27

  Fragments arranged by size: 

                1150      32      27
 
HindIII A'AGCT_T

Cuts at:      0     135    1209 
   Size:        135    1074
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     264    1209 
   Size:        134     130     945

  Fragments arranged by size: 

                 945     134     130
 
MscI TGG'CCA

Cuts at:      0      49    1209 
   Size:         49    1160
 
MspA1I CmG'CkG

Cuts at:      0     984    1188    1209 
   Size:        984     204      21

  Fragments arranged by size: 

                 984     204      21
 
NaeI GCC'GGC

Cuts at:      0      83    1209 
   Size:         83    1126
 
NgoMIV G'CCGG_C

Cuts at:      0      81    1209 
   Size:         81    1128
 
NspI r_CATG'y

Cuts at:      0     178     531    1209 
   Size:        178     353     678

  Fragments arranged by size: 

                 678     353     178
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     873    1209 
   Size:        873     336
 
PfoI T'CCnGG_A

Cuts at:      0      68    1209 
   Size:         68    1141
 
PpuMI rG'GwC_Cy

Cuts at:      0     354    1052    1146    1209 
   Size:        354     698      94      63

  Fragments arranged by size: 

                 698     354      94      63
 
PstI C_TGCA'G

Cuts at:      0     149    1066    1209 
   Size:        149     917     143

  Fragments arranged by size: 

                 917     149     143
 
PvuII CAG'CTG

Cuts at:      0    1188    1209 
   Size:       1188      21
 
SanDI GG'GwC_CC

Cuts at:      0    1052    1209 
   Size:       1052     157
 
SexAI A'CCwGG_T

Cuts at:      0     704     815    1034    1209 
   Size:        704     111     219     175

  Fragments arranged by size: 

                 704     219     175     111
 
SfcI C'TryA_G

Cuts at:      0     145    1062    1104    1209 
   Size:        145     917      42     105

  Fragments arranged by size: 

                 917     145     105      42
 
SmlI C'TyrA_G

Cuts at:      0     478    1209 
   Size:        478     731
 
StuI AGG'CCT

Cuts at:      0     508    1209 
   Size:        508     701
 
StyI C'CwwG_G

Cuts at:      0     323    1209 
   Size:        323     886
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     BanI    BanII     BbsI    BbvCI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    Hin4I  HindIII     MmeI     MscI
   MspA1I     NaeI   NgoMIV     NspI    PflMI     PfoI    PpuMI     PstI
    PvuII    SanDI    SexAI     SfcI     SmlI     StuI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI    BsaAI
    BsaBI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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