Restriction map for pF1KSDA1165
 (Circular) (Six-base) MAPSORT of: pF1KSDA1165.seq  Check: 8748  from: 1  to: 3865



pF1KSDA1165  3865 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:    901     901 
   Size:       3865
 
Acc65I G'GTAC_C

Cuts at:    879     879 
   Size:       3865
 
AclI AA'CG_TT

Cuts at:   3705    3705 
   Size:       3865
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    239     311     483     750    1688    2120    2869    3137     239 
   Size:         72     172     267     938     432     749     268     967

  Fragments arranged by size: 

                 967     938     749     432     268     267     172      72
            
 
AflIII A'CryG_T

Cuts at:    800    1215    2321    3298     800 
   Size:        415    1106     977    1367

  Fragments arranged by size: 

                1367    1106     977     415
 
AgeI A'CCGG_T

Cuts at:   2253    2253 
   Size:       3865
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    559     559 
   Size:       3865
 
AlwNI CAG_nnn'CTG

Cuts at:   1168    2737    1168 
   Size:       1569    2296
 
ApaLI G'TGCA_C

Cuts at:   2635    2635 
   Size:       3865
 
ApoI r'AATT_y

Cuts at:    464     867    3218    3474     464 
   Size:        403    2351     256     855

  Fragments arranged by size: 

                2351     855     403     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3865
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3865
 
AvaI C'yCGr_G

Cuts at:    883     883 
   Size:       3865
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    323     356     323 
   Size:         33    3832
 
BamHI G'GATC_C

Cuts at:    668     888     668 
   Size:        220    3645
 
BanI G'GyrC_C

Cuts at:    879    1293    1541    1576     879 
   Size:        414     248      35    3168

  Fragments arranged by size: 

                3168     414     248      35
 
BanII G_rGCy'C

Cuts at:    877    1907     877 
   Size:       1030    2835
 
BbeI G_GCGC'C

Cuts at:   1297    1545    1297 
   Size:        248    3617
 
BbsI GAAGACnn'nnnn_

Cuts at:    245     245 
   Size:       3865
 
BciVI GTATCCnnnnn_n'

Cuts at:   1761    2524    3865    1761 
   Size:        763    1341    1761

  Fragments arranged by size: 

                1761    1341     763
 
BclI T'GATC_A

Cuts at:    659    1383     659 
   Size:        724    3141
 
BglII A'GATC_T

Cuts at:   1378    3417    1378 
   Size:       2039    1826
 
BlpI GC'TnA_GC

Cuts at:    975     975 
   Size:       3865
 
Bme1580I G_kGCm'C

Cuts at:   1488    1581    2639    1488 
   Size:         93    1058    2714

  Fragments arranged by size: 

                2714    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    158    1075    1156    1491    3637     158 
   Size:        917      81     335    2146     386

  Fragments arranged by size: 

                2146     917     386     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    201    2230     201 
   Size:       2029    1836
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1039    1918    2412    2710    1039 
   Size:        879     494     298    2194

  Fragments arranged by size: 

                2194     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3569    3569 
   Size:       3865
 
BsaAI yAC'GTr

Cuts at:    855    1216     855 
   Size:        361    3504
 
BsaBI GATnn'nnATC

Cuts at:      6    1377    3416       6 
   Size:       1371    2039     455

  Fragments arranged by size: 

                2039    1371     455
 
BsaHI Gr'CG_yC

Cuts at:   1294    1542    2246    3742    1294 
   Size:        248     704    1496    1417

  Fragments arranged by size: 

                1496    1417     704     248
 
BsaWI w'CCGG_w

Cuts at:   1052    1573    2253    2527    2674    3085    1052 
   Size:        521     680     274     147     411    1832

  Fragments arranged by size: 

                1832     680     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    463     493     463 
   Size:         30    3835
 
BseYI C'CCAG_C

Cuts at:    225    1288    2046    2625    3121     225 
   Size:       1063     758     579     496     969

  Fragments arranged by size: 

                1063     969     758     579     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    262     262 
   Size:       3865
 
BsiEI CG_ry'CG

Cuts at:    104    1405    1451    2661     104 
   Size:       1301      46    1210    1308

  Fragments arranged by size: 

                1308    1301    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    877    1655    1845    2639     877 
   Size:        778     190     794    2103

  Fragments arranged by size: 

                2103     794     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    877    1488    1581    1655    1845    1907    2639     877 
   Size:        611      93      74     190      62     732    2103

  Fragments arranged by size: 

                2103     732     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3085    3085 
   Size:       3865
 
BspHI T'CATG_A

Cuts at:   3041    3041 
   Size:       3865
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    913    1127    1429    1810     913 
   Size:        214     302     381    2968

  Fragments arranged by size: 

                2968     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2155    2209    3695    3860      50 
   Size:       2105      54    1486     165      55

  Fragments arranged by size: 

                2105    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    437    1775     437 
   Size:       1338    2527
 
BsrFI r'CCGG_y

Cuts at:   1861    2042    2253    1861 
   Size:        181     211    3473

  Fragments arranged by size: 

                3473     211     181
 
BssSI C'ACGA_G

Cuts at:   2134    2494    2134 
   Size:        360    3505
 
BstAPI GCAn_nnn'nTGC

Cuts at:    240    1240     240 
   Size:       1000    2865
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       3865
 
BstYI r'GATC_y

Cuts at:      1     668     888    1355    1378    1713    1959    2973    3059 
   Size:        667     220     467      23     335     246    1014      86

Cuts at:   3059    3071    3417       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1014     667     467     449     346     335     246     220
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       3865
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1813    1994    2157    1813 
   Size:        181     163    3521

  Fragments arranged by size: 

                3521     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1106    1106 
   Size:       3865
 
DraI TTT'AAA

Cuts at:    862     862 
   Size:       3865
 
DrdI GACnn_nn'nnGTC

Cuts at:   1569    2429    1569 
   Size:        860    3005
 
EaeI y'GGCC_r

Cuts at:   1448    1622    2013    2040    3387    1448 
   Size:        174     391      27    1347    1926

  Fragments arranged by size: 

                1926    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   1448    1448 
   Size:       3865
 
EarI CTCTTCn'nnn_

Cuts at:    170     285     395     470    1886    2096     170 
   Size:        115     110      75    1416     210    1939

  Fragments arranged by size: 

                1939    1416     210     115     110      75
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2071    2383    2529    3675    2071 
   Size:        312     146    1146    2261

  Fragments arranged by size: 

                2261    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:    875     875 
   Size:       3865
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    239     311     483     750    1688    2120    2869    3137     239 
   Size:         72     172     267     938     432     749     268     967

  Fragments arranged by size: 

                 967     938     749     432     268     267     172      72
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1002    1002 
   Size:       3865
 
EcoRI G'AATT_C

Cuts at:    464     867     464 
   Size:        403    3462
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1882    1882 
   Size:       3865
 
FspI TGC'GCA

Cuts at:   1644    1644 
   Size:       3865
 
HaeII r_GCGC'y

Cuts at:    156    1297    1545    2569    3548     156 
   Size:       1141     248    1024     979     473

  Fragments arranged by size: 

                1141    1024     979     473     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1705    1735    1737    1767    1705 
   Size:         30       2      30    3803

  Fragments arranged by size: 

                3803      30      30       2
 
HincII GTy'rAC

Cuts at:    902     902 
   Size:       3865
 
KasI G'GCGC_C

Cuts at:   1293    1541    1293 
   Size:        248    3617
 
KpnI G_GTAC'C

Cuts at:    883     883 
   Size:       3865
 
MluI A'CGCG_T

Cuts at:   3298    3298 
   Size:       3865
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2536    2720    2536 
   Size:        184    3681
 
MscI TGG'CCA

Cuts at:   1624    1624 
   Size:       3865
 
MslI CAynn'nnrTG

Cuts at:    541    1852    1979     541 
   Size:       1311     127    2427

  Fragments arranged by size: 

                2427    1311     127
 
MspA1I CmG'CkG

Cuts at:    439     820     974    1288    1648    2663    2908     439 
   Size:        381     154     314     360    1015     245    1396

  Fragments arranged by size: 

                1396    1015     381     360     314     245     154
 
NaeI GCC'GGC

Cuts at:   2044    2044 
   Size:       3865
 
NarI GG'CG_CC

Cuts at:   1294    1542    1294 
   Size:        248    3617
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       3865
 
NgoMIV G'CCGG_C

Cuts at:   2042    2042 
   Size:       3865
 
NruI TCG'CGA

Cuts at:   2226    2226 
   Size:       3865
 
NspI r_CATG'y

Cuts at:    804     916    1416    2325     804 
   Size:        112     500     909    2344

  Fragments arranged by size: 

                2344     909     500     112
 
PciI A'CATG_T

Cuts at:    800    2321     800 
   Size:       1521    2344
 
PmeI GTTT'AAAC

Cuts at:    862     862 
   Size:       3865
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3004    3036    3004 
   Size:         32    3833
 
PstI C_TGCA'G

Cuts at:    444     910    1595     444 
   Size:        466     685    2714

  Fragments arranged by size: 

                2714     685     466
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3865
 
PvuII CAG'CTG

Cuts at:    439     820    1288    1648     439 
   Size:        381     468     360    2656

  Fragments arranged by size: 

                2656     468     381     360
 
RsrII CG'GwC_CG

Cuts at:   2058    2058 
   Size:       3865
 
SacI G_AGCT'C

Cuts at:    877     877 
   Size:       3865
 
SalI G'TCGA_C

Cuts at:    900     900 
   Size:       3865
 
SapI GCTCTTCn'nnn_

Cuts at:   1886    2096    1886 
   Size:        210    3655
 
SbfI CC_TGCA'GG

Cuts at:    910     910 
   Size:       3865
 
ScaI AGT'ACT

Cuts at:   3382    3382 
   Size:       3865
 
SexAI A'CCwGG_T

Cuts at:    158     158 
   Size:       3865
 
SfcI C'TryA_G

Cuts at:     33     200     372     440     906    1591    2586    2777      33 
   Size:        167     172      68     466     685     995     191    1121

  Fragments arranged by size: 

                1121     995     685     466     191     172     167      68
            
 
SfoI GGC'GCC

Cuts at:   1295    1543    1295 
   Size:        248    3617
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3865
 
SmaI CCC'GGG

Cuts at:    885     885 
   Size:       3865
 
SmlI C'TyrA_G

Cuts at:   1018    1933    2427    2689    1018 
   Size:        915     494     262    2194

  Fragments arranged by size: 

                2194     915     494     262
 
SnaBI TAC'GTA

Cuts at:    855     855 
   Size:       3865
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3865
 
SphI G_CATG'C

Cuts at:    916    1416     916 
   Size:        500    3365
 
StyI C'CwwG_G

Cuts at:    125     997    1351     125 
   Size:        872     354    2639

  Fragments arranged by size: 

                2639     872     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1446    1579    2038    2249    1446 
   Size:        133     459     211    3062

  Fragments arranged by size: 

                3062     459     211     133
 
TatI w'GTAC_w

Cuts at:    744    3380     744 
   Size:       2636    1229
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    463     495    3382    3414     463 
   Size:         32    2887      32     914

  Fragments arranged by size: 

                2887     914      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1660    1660 
   Size:       3865
 
XbaI T'CTAG_A

Cuts at:    894     894 
   Size:       3865
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    145    1358     145 
   Size:       1213    2652
 
XmaI C'CCGG_G

Cuts at:    883     883 
   Size:       3865
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BpmI   Bpu10I    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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