Restriction map for pF1KSDA1133
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       BalI        
BcgI        BfrI        BlpI        Bpu1102I    BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KpnI        MfeI        MluI        MluNI       
MscI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI         1405  GTAGAC               GT^[AC][GT]AC
AccIII       2379  TCCGGA               T^CCGGA
AcsI          444  GAATTC               [GA]^AATT[CT]
AcyI          518  GGCGCC               G[GA]^CG[CT]C
AgeI          638  ACCGGT               A^CCGGT
AlwNI        2082  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1788  CCTCAGG              CC^T[ACGT]AGG
ApoI          444  GAATTC               [GA]^AATT[CT]
AspI          434  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         117  CCTAGG               C^CTAGG
BamHI         311  GGATCC               G^GATCC
BanI          518  GGCGCC               G^G[CT][GA]CC
BbeI          518  GGCGCC               GGCGC^C
BbsI          217  GAAGAC               GAAGAC(2/6)
BciVI        2283  GGATAC               GTATCC(6/5)
BclI          195  TGATCA               T^GATCA
BglII        2473  AGATCT               A^GATCT
BlnI          117  CCTAGG               C^CTAGG
BpuAI         217  GAAGAC               GAAGAC(2/6)
BsaBI         273  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         518  GGCGCC               G[GA]^CG[CT]C
BseAI        2379  TCCGGA               T^CCGGA
BsiEI         807  CGGCCG               CG[GA][CT]^CG
BspEI        2379  TCCGGA               T^CCGGA
BssSI        1813  CACGAG               CACGAG(-5/-1)
BstAPI       2081  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        783  GGTGACC              G^GT[ACGT]ACC
BstPI         783  GGTGACC              G^GT[ACGT]ACC
BstXI         581  CCATCTGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1788  CCTCAGG              CC^T[ACGT]AGG
EagI          807  CGGCCG               C^GGCCG
EclXI         807  CGGCCG               C^GGCCG
Eco52I        807  CGGCCG               C^GGCCG
Eco81I       1788  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       783  GGTGACC              G^GT[ACGT]ACC
EcoRI         444  GAATTC               G^AATTC
FbaI          195  TGATCA               T^GATCA
Hin1I         518  GGCGCC               G[GA]^CG[CT]C
HincII        283  GTCAAC               GT[CT]^[GA]AC
HindII        283  GTCAAC               GT[CT]^[GA]AC
KasI          518  GGCGCC               G^GCGCC
KspI         2064  CCGCGG               CCGC^GG
MamI          273  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2379  TCCGGA               T^CCGGA
MseI          266  TTAA                 T^TAA
NarI          518  GGCGCC               GG^CGCC
NcoI          893  CCATGG               C^CATGG
PflFI         434  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         975  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         638  ACCGGT               A^CCGGT
SacI         1594  GAGCTC               GAGCT^C
SacII        2064  CCGCGG               CCGC^GG
SapI         1221  GCTCTTC              GCTCTTC(1/4)
SfoI          518  GGCGCC               GGC^GCC
Tru9I         266  TTAA                 T^TAA
Tth111I       434  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        975  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          474  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BbrPI         764  CACGTG               CAC^GTG
             1240  CACGTG              
BbvCI        1128  CCTCAGC              CCTCAGC(-5/-2)
             1467  CCTCAGC             
BglI         1519  GCCAGTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2452  GCCACTGAGGC         
BsaAI         764  CACGTG               [CT]AC^GT[GA]
             1240  CACGTG              
BsaWI         638  ACCGGT               [AT]^CCGG[AT]
             2379  TCCGGA              
BstYI         311  GGATCC               [GA]^GATC[CT]
             2473  AGATCT              
HaeII         518  GGCGCC               [GA]GCGC^[CT]
             1870  AGCGCC              
HgaI          434  GACGC                GACGC(5/10)
             2271  GACGC               
MflI          311  GGATCC               [GA]^GATC[CT]
             2473  AGATCT              
MslI         1230  CACCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1609  CACAGTCATG          
PaeR7I        302  CTCGAG               C^TCGAG
             1759  CTCGAG              
PmaCI         764  CACGTG               CAC^GTG
             1240  CACGTG              
PmlI          764  CACGTG               CAC^GTG
             1240  CACGTG              
SfaNI         259  GATGC                GCATC(5/9)
             2183  GCATC               
Tsp509I        89  AATT                 ^AATT
              445  AATT                
XhoI          302  CTCGAG               C^TCGAG
             1759  CTCGAG              
XhoII         311  GGATCC               [GA]^GATC[CT]
             2473  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        Alw44I      
AlwI        ApaI        ApaLI       AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        SmaI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XmaCI       XmaI        
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