Restriction map for pF1KSDA1133
 (Linear) (Six-base) MAPSORT of: KIAA1133.seq  Check: 9216  from: 1  to: 2508



KIAA1133.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     694    1687    2046    2508 
   Size:        694     993     359     462

  Fragments arranged by size: 

                 993     694     462     359
 
AccI GT'mk_AC

Cuts at:      0    1406    2508 
   Size:       1406    1102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     445    1269    2508 
   Size:        236     209     824    1239

  Fragments arranged by size: 

                1239     824     236     209
 
AgeI A'CCGG_T

Cuts at:      0     638    2508 
   Size:        638    1870
 
AleI CACnn'nnGTG

Cuts at:      0    1234    2508 
   Size:       1234    1274
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2087    2508 
   Size:       2087     421
 
ApaI G_GGCC'C

Cuts at:      0     543    1183    1648    1733    1910    2166    2358    2508 
   Size:        543     640     465      85     177     256     192     150

  Fragments arranged by size: 

                 640     543     465     256     192     177     150      85
            
 
ApaLI G'TGCA_C

Cuts at:      0     814    1237    1606    2508 
   Size:        814     423     369     902

  Fragments arranged by size: 

                 902     814     423     369
 
ApoI r'AATT_y

Cuts at:      0     444    2508 
   Size:        444    2064
 
AvaI C'yCGr_G

Cuts at:      0     302    1175    1536    1545    1588    1659    1759    2158 
   Size:        302     873     361       9      43      71     100     399

Cuts at:   2158    2165    2318    2499    2508 
   Size:          7     153     181       9

  Fragments arranged by size: 

                 873     399     361     302     181     153     100      71
                  43       9       9       7
 
AvrII C'CTAG_G

Cuts at:      0     117    2508 
   Size:        117    2391
 
BamHI G'GATC_C

Cuts at:      0     311    2508 
   Size:        311    2197
 
BanI G'GyrC_C

Cuts at:      0     518    2508 
   Size:        518    1990
 
BanII G_rGCy'C

Cuts at:      0     214     543    1183    1510    1537    1579    1598    1648 
   Size:        214     329     640     327      27      42      19      50

Cuts at:   1648    1733    1760    1847    1910    1927    1949    2166    2337 
   Size:         85      27      87      63      17      22     217     171

Cuts at:   2337    2358    2508 
   Size:         21     150

  Fragments arranged by size: 

                 640     329     327     217     214     171     150      87
                  85      63      50      42      27      27      22      21
                  19      17
 
BbeI G_GCGC'C

Cuts at:      0     522    2508 
   Size:        522    1986
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     224    2508 
   Size:        224    2284
 
BbvCI CC'TCA_GC

Cuts at:      0    1129    1468    2508 
   Size:       1129     339    1040

  Fragments arranged by size: 

                1129    1040     339
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2277    2508 
   Size:       2277     231
 
BclI T'GATC_A

Cuts at:      0     195    2508 
   Size:        195    2313
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1525    2458    2508 
   Size:       1525     933      50

  Fragments arranged by size: 

                1525     933      50
 
BglII A'GATC_T

Cuts at:      0    2473    2508 
   Size:       2473      35
 
Bme1580I G_kGCm'C

Cuts at:      0     543     818    1183    1241    1610    1648    1733    1910 
   Size:        543     275     365      58     369      38      85     177

Cuts at:   1910    2166    2358    2421    2508 
   Size:        256     192      63      87

  Fragments arranged by size: 

                 543     369     365     275     256     192     177      87
                  85      63      58      38
 
BmgBI CAC'GTC

Cuts at:      0     569    1277    2508 
   Size:        569     708    1231

  Fragments arranged by size: 

                1231     708     569
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      19    1083    1944    2508 
   Size:         19    1064     861     564

  Fragments arranged by size: 

                1064     861     564      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2121    2508 
   Size:       2121     387
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610    1024    1282    1374    1708    1738    2407    2508 
   Size:        610     414     258      92     334      30     669     101

  Fragments arranged by size: 

                 669     610     414     334     258     101      92      30
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1129    1468    2173    2508 
   Size:       1129     339     705     335

  Fragments arranged by size: 

                1129     705     339     335
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211    2508 
   Size:        211    2297
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     390     742    1090    2508 
   Size:        390     352     348    1418

  Fragments arranged by size: 

                1418     390     352     348
 
BsaAI yAC'GTr

Cuts at:      0     766    1242    2508 
   Size:        766     476    1266

  Fragments arranged by size: 

                1266     766     476
 
BsaBI GATnn'nnATC

Cuts at:      0     277    2508 
   Size:        277    2231
 
BsaHI Gr'CG_yC

Cuts at:      0     519    2508 
   Size:        519    1989
 
BsaWI w'CCGG_w

Cuts at:      0     638    2379    2508 
   Size:        638    1741     129

  Fragments arranged by size: 

                1741     638     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1138    1168    1372    1402    1497    1527    1936    1966 
   Size:       1138      30     204      30      95      30     409      30

Cuts at:   1966    2508 
   Size:        542

  Fragments arranged by size: 

                1138     542     409     204      95      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     317     625    1606    1701    2341    2398    2508 
   Size:        317     308     981      95     640      57     110

  Fragments arranged by size: 

                 981     640     317     308     110      95      57
 
BseYI C'CCAG_C

Cuts at:      0      56     834    1023    1621    1847    1857    2508 
   Size:         56     778     189     598     226      10     651

  Fragments arranged by size: 

                 778     651     598     226     189      56      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     487    1335    1395    2137    2508 
   Size:        275     212     848      60     742     371

  Fragments arranged by size: 

                 848     742     371     275     212      60
 
BsiEI CG_ry'CG

Cuts at:      0     810    2508 
   Size:        810    1698
 
BsiHKAI G_wGCw'C

Cuts at:      0     818    1010    1241    1598    1610    1724    2508 
   Size:        818     192     231     357      12     114     784

  Fragments arranged by size: 

                 818     784     357     231     192     114      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     214     543     818    1010    1183    1241    1510    1537 
   Size:        214     329     275     192     173      58     269      27

Cuts at:   1537    1579    1598    1610    1648    1724    1733    1760    1847 
   Size:         42      19      12      38      76       9      27      87

Cuts at:   1847    1910    1927    1949    2166    2337    2358    2421    2508 
   Size:         63      17      22     217     171      21      63      87

  Fragments arranged by size: 

                 329     275     269     217     214     192     173     171
                  87      87      76      63      63      58      42      38
                  27      27      22      21      19      17      12       9
            
 
BspEI T'CCGG_A

Cuts at:      0    2379    2508 
   Size:       2379     129
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     694    1566    1687    2046    2508 
   Size:        694     872     121     359     462

  Fragments arranged by size: 

                 872     694     462     359     121
 
BsrBI CCG'CTC

Cuts at:      0     225     988    1205    1268    1307    2331    2508 
   Size:        225     763     217      63      39    1024     177

  Fragments arranged by size: 

                1024     763     225     217     177      63      39
 
BsrFI r'CCGG_y

Cuts at:      0     234     638     792     899    2508 
   Size:        234     404     154     107    1609

  Fragments arranged by size: 

                1609     404     234     154     107
 
BssSI C'ACGA_G

Cuts at:      0    1813    2508 
   Size:       1813     695
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2087    2508 
   Size:       2087     421
 
BstEII G'GTnAC_C

Cuts at:      0     783    2508 
   Size:        783    1725
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588    2508 
   Size:        588    1920
 
BstYI r'GATC_y

Cuts at:      0     311    2473    2508 
   Size:        311    2162      35

  Fragments arranged by size: 

                2162     311      35
 
Bsu36I CC'TnA_GG

Cuts at:      0    1789    2508 
   Size:       1789     719
 
BtgI C'CryG_G

Cuts at:      0     867     893    1232    2064    2290    2508 
   Size:        867      26     339     832     226     218

  Fragments arranged by size: 

                 867     832     339     226     218      26
 
BtsI GCAGTG_nn'

Cuts at:      0    1215    1323    2447    2508 
   Size:       1215     108    1124      61

  Fragments arranged by size: 

                1215    1124     108      61
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1035    1070    2508 
   Size:       1035      35    1438

  Fragments arranged by size: 

                1438    1035      35
 
EaeI y'GGCC_r

Cuts at:      0     807     983    1056    1299    1328    1371    1639    2508 
   Size:        807     176      73     243      29      43     268     869

  Fragments arranged by size: 

                 869     807     268     243     176      73      43      29
            
 
EagI C'GGCC_G

Cuts at:      0     807    2508 
   Size:        807    1701
 
EarI CTCTTCn'nnn_

Cuts at:      0      29    1228    2042    2318    2508 
   Size:         29    1199     814     276     190

  Fragments arranged by size: 

                1199     814     276     190      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2087    2508 
   Size:       2087     421
 
EcoICRI GAG'CTC

Cuts at:      0    1596    2508 
   Size:       1596     912
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     445     610    1024    1269    1282    1374    1708 
   Size:        236     209     165     414     245      13      92     334

Cuts at:   1708    1738    2407    2508 
   Size:         30     669     101

  Fragments arranged by size: 

                 669     414     334     245     236     209     165     101
                  92      30      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     121    1038    1918    2508 
   Size:        121     917     880     590

  Fragments arranged by size: 

                 917     880     590     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0     539     540    1125    1179    1555    1645    1729    1796 
   Size:        539       1     585      54     376      90      84      67

Cuts at:   1796    1906    1907    2162    2169    2243    2304    2354    2355 
   Size:        110       1     255       7      74      61      50       1

Cuts at:   2355    2508 
   Size:        153

  Fragments arranged by size: 

                 585     539     376     255     153     110      90      84
                  74      67      61      54      50       7       1       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0     444    2508 
   Size:        444    2064
 
HaeII r_GCGC'y

Cuts at:      0     522    1874    2508 
   Size:        522    1352     634

  Fragments arranged by size: 

                1352     634     522
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     296     328    1138    1170    1497    1529    1657    1689 
   Size:        296      32     810      32     327      32     128      32

Cuts at:   1689    1807    1839    1936    1968    2508 
   Size:        118      32      97      32     540

  Fragments arranged by size: 

                 810     540     327     296     128     118      97      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     285    2508 
   Size:        285    2223
 
KasI G'GCGC_C

Cuts at:      0     518    2508 
   Size:        518    1990
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     596    2508 
   Size:        596    1912
 
MslI CAynn'nnrTG

Cuts at:      0    1234    1613    2508 
   Size:       1234     379     895

  Fragments arranged by size: 

                1234     895     379
 
MspA1I CmG'CkG

Cuts at:      0     144     201     995    1289    1882    2066    2084    2508 
   Size:        144      57     794     294     593     184      18     424

  Fragments arranged by size: 

                 794     593     424     294     184     144      57      18
            
 
NarI GG'CG_CC

Cuts at:      0     519    2508 
   Size:        519    1989
 
NcoI C'CATG_G

Cuts at:      0     893    2508 
   Size:        893    1615
 
PasI CC'CwG_GG

Cuts at:      0    1965    2508 
   Size:       1965     543
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     981    2508 
   Size:        981    1527
 
PfoI T'CCnGG_A

Cuts at:      0    2423    2508 
   Size:       2423      85
 
PmlI CAC'GTG

Cuts at:      0     766    1242    2508 
   Size:        766     476    1266

  Fragments arranged by size: 

                1266     766     476
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     196     228    2508 
   Size:        196      32    2280

  Fragments arranged by size: 

                2280     196      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1555    1796    2169    2243    2508 
   Size:       1555     241     373      74     265

  Fragments arranged by size: 

                1555     373     265     241      74
 
PspOMI G'GGCC_C

Cuts at:      0     539    1179    1644    1729    1906    2162    2354    2508 
   Size:        539     640     465      85     177     256     192     154

  Fragments arranged by size: 

                 640     539     465     256     192     177     154      85
            
 
PspXI vC'TCGA_Gb

Cuts at:      0     302    1759    2508 
   Size:        302    1457     749

  Fragments arranged by size: 

                1457     749     302
 
PstI C_TGCA'G

Cuts at:      0     159     677    1684    2466    2508 
   Size:        159     518    1007     782      42

  Fragments arranged by size: 

                1007     782     518     159      42
 
PvuII CAG'CTG

Cuts at:      0     201    1289    1882    2508 
   Size:        201    1088     593     626

  Fragments arranged by size: 

                1088     626     593     201
 
SacI G_AGCT'C

Cuts at:      0    1598    2508 
   Size:       1598     910
 
SacII CC_GC'GG

Cuts at:      0    2067    2508 
   Size:       2067     441
 
SanDI GG'GwC_CC

Cuts at:      0    2243    2508 
   Size:       2243     265
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1228    2508 
   Size:       1228    1280
 
SfcI C'TryA_G

Cuts at:      0     155     673    1680    1698    2462    2508 
   Size:        155     518    1007      18     764      46

  Fragments arranged by size: 

                1007     764     518     155      46      18
 
SfoI GGC'GCC

Cuts at:      0     520    2508 
   Size:        520    1988
 
SmaI CCC'GGG

Cuts at:      0    1177    2160    2167    2320    2501    2508 
   Size:       1177     983       7     153     181       7

  Fragments arranged by size: 

                1177     983     181     153       7       7
 
SmlI C'TyrA_G

Cuts at:      0     226     302    1759    2508 
   Size:        226      76    1457     749

  Fragments arranged by size: 

                1457     749     226      76
 
StyI C'CwwG_G

Cuts at:      0     117     389     893    1070    1636    2238    2508 
   Size:        117     272     504     177     566     602     270

  Fragments arranged by size: 

                 602     566     504     272     270     177     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      20    2484    2508 
   Size:         20    2464      24

  Fragments arranged by size: 

                2464      24      20
 
TatI w'GTAC_w

Cuts at:      0     138     596     631    2120    2508 
   Size:        138     458      35    1489     388

  Fragments arranged by size: 

                1489     458     388     138      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1372    1404    2508 
   Size:       1372      32    1104

  Fragments arranged by size: 

                1372    1104      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     437    2508 
   Size:        437    2071
 
XbaI T'CTAG_A

Cuts at:      0     474    2508 
   Size:        474    2034
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     456    1398    1683    2508 
   Size:        456     942     285     825

  Fragments arranged by size: 

                 942     825     456     285
 
XhoI C'TCGA_G

Cuts at:      0     302    1759    2508 
   Size:        302    1457     749

  Fragments arranged by size: 

                1457     749     302
 
XmaI C'CCGG_G

Cuts at:      0    1175    2158    2165    2318    2499    2508 
   Size:       1175     983       7     153     181       9

  Fragments arranged by size: 

                1175     983     181     153       9       7
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     KasI     MmeI     MslI   MspA1I     NarI     NcoI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SapI     SfcI     SfoI     SmaI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI     AscI     AseI    AsiSI     BaeI     BcgI    BfrBI     BlpI
     BmtI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI   BssHII
    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI    PshAI     PsiI     PsrI
     PvuI    RsrII     SalI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XmnI     ZraI
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