Restriction map for pF1KSDA1112
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       BamHI       BbrPI       BciVI       BclI        
BfrI        BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
Ksp632I     MfeI        MluI        MroI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tsp509I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         655  GACGTC               GACGT^C
AlwI          873  GATCC                GGATC(4/5)
Aor51HI      1784  AGCGCT               AGC^GCT
ApaI          110  GGGCCC               GGGCC^C
AspI          610  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         163  CCTAGG               C^CTAGG
BalI          403  TGGCCA               TGG^CCA
BbeI         1732  GGCGCC               GGCGC^C
BbsI          216  GAAGAC               GAAGAC(2/6)
BglI          784  GCCCCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          163  CCTAGG               C^CTAGG
BlpI         1182  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1182  GCTCAGC              GC^T[ACGT]AGC
BpuAI         216  GAAGAC               GAAGAC(2/6)
BsaAI        1192  TACGTG               [CT]AC^GT[GA]
BsaBI        1233  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1688  GAGACC               GGTCTC(1/5)
BsaWI        1266  TCCGGT               [AT]^CCGG[AT]
BsmI         1884  GAATGC               GAATGC(1/-1)
BspLU11I      956  ACATGT               A^CATGT
BssHII        948  GCGCGC               G^CGCGC
BstAPI       1872  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          92  CCACTGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1182  GCTCAGC              GC^T[ACGT]AGC
DrdI          448  GACACAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1784  AGCGCT               AGC^GCT
EcoNI        1799  CCTTTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          781  GACGC                GACGC(5/10)
KasI         1732  GGCGCC               G^GCGCC
KspI          770  CCGCGG               CCGC^GG
MamI         1233  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         403  TGGCCA               TGG^CCA
MscI          403  TGGCCA               TGG^CCA
NarI         1732  GGCGCC               GG^CGCC
NcoI          620  CCATGG               C^CATGG
PaeR7I       1520  CTCGAG               C^TCGAG
PflFI         610  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         630  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         855  GACCAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         770  CCGCGG               CCGC^GG
ScaI         1130  AGTACT               AGT^ACT
SfoI         1732  GGCGCC               GGC^GCC
SphI         1365  GCATGC               GCATG^C
Tth111I       610  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        630  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1520  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Alw44I        280  GTGCAC               G^TGCAC
             1758  GTGCAC              
ApaLI         280  GTGCAC               G^TGCAC
             1758  GTGCAC              
BanI         1084  GGTGCC               G^G[CT][GA]CC
             1732  GGCGCC              
BbvCI         876  CCTCAGC              CCTCAGC(-5/-2)
              747  GCTGAGG             
BcgI         1077  CGAGCTGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1425  CGACAAGATTGC        
BfaI          164  CTAG                 C^TAG
              347  CTAG                
BsrFI        1214  GCCGGC               [GA]^CCGG[CT]
             1634  GCCGGC              
BsrI         1460  ACTGG                ACTGG(1/-1)
               32  CCAGT               
Cfr10I       1214  GCCGGC               [GA]^CCGG[CT]
             1634  GCCGGC              
EcoT14I       163  CCTAGG               C^C[AT][AT]GG
              620  CCATGG              
MaeI          164  CTAG                 C^TAG
              347  CTAG                
NaeI         1214  GCCGGC               GCC^GGC
             1634  GCCGGC              
NgoMIV       1214  GCCGGC               G^CCGGC
             1634  GCCGGC              
NspI          956  ACATGT               [GA]CATG^[CT]
             1365  GCATGC              
PpuMI         485  AGGACCT              [GA]G^G[AT]CC[CT]
             1598  AGGACCT             
StyI          163  CCTAGG               C^C[AT][AT]GG
              620  CCATGG              
TfiI          339  GAATC                G^A[AT]TC
              756  GAATC               
XcmI         1148  CCAACGCCTGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1370  CCATGAAGAACGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       Asp700I     AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BglII       
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       XmnI        
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