Restriction map for pF1KSDA1112
 (Linear) (Six-base) MAPSORT of: KIAA1112.seq  Check: 3424  from: 1  to: 2010



KIAA1112.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      10     914    1760    1809    2010 
   Size:         10     904     846      49     201

  Fragments arranged by size: 

                 904     846     201      49      10
 
AatII G_ACGT'C

Cuts at:      0     659    2010 
   Size:        659    1351
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    2010 
   Size:        617    1393
 
AfeI AGC'GCT

Cuts at:      0    1786    2010 
   Size:       1786     224
 
AflIII A'CryG_T

Cuts at:      0     956    1193    1616    2010 
   Size:        956     237     423     394

  Fragments arranged by size: 

                 956     423     394     237
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445    2010 
   Size:        445    1565
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1033    1065    2010 
   Size:       1033      32     945

  Fragments arranged by size: 

                1033     945      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     136     424     636    2010 
   Size:        136     288     212    1374

  Fragments arranged by size: 

                1374     288     212     136
 
ApaI G_GGCC'C

Cuts at:      0     114    2010 
   Size:        114    1896
 
ApaLI G'TGCA_C

Cuts at:      0     280    1758    2010 
   Size:        280    1478     252

  Fragments arranged by size: 

                1478     280     252
 
AvaI C'yCGr_G

Cuts at:      0    1506    1520    1771    2010 
   Size:       1506      14     251     239

  Fragments arranged by size: 

                1506     251     239      14
 
AvrII C'CTAG_G

Cuts at:      0     163    2010 
   Size:        163    1847
 
BanI G'GyrC_C

Cuts at:      0    1084    1732    2010 
   Size:       1084     648     278

  Fragments arranged by size: 

                1084     648     278
 
BanII G_rGCy'C

Cuts at:      0     114     444     569     770    1166    1450    1492    2010 
   Size:        114     330     125     201     396     284      42     518

  Fragments arranged by size: 

                 518     396     330     284     201     125     114      42
            
 
BbeI G_GCGC'C

Cuts at:      0    1736    2010 
   Size:       1736     274
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     223    2010 
   Size:        223    1787
 
BbvCI CC'TCA_GC

Cuts at:      0     748     877    2010 
   Size:        748     129    1133

  Fragments arranged by size: 

                1133     748     129
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1066    1100    1414    1448    2010 
   Size:       1066      34     314      34     562

  Fragments arranged by size: 

                1066     562     314      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     790    2010 
   Size:        790    1220
 
BglII A'GATC_T

Cuts at:      0    1118    1277    1473    2010 
   Size:       1118     159     196     537

  Fragments arranged by size: 

                1118     537     196     159
 
BlpI GC'TnA_GC

Cuts at:      0    1183    2010 
   Size:       1183     827
 
Bme1580I G_kGCm'C

Cuts at:      0     114     284    1762    2010 
   Size:        114     170    1478     248

  Fragments arranged by size: 

                1478     248     170     114
 
BmgBI CAC'GTC

Cuts at:      0     252    1617    2010 
   Size:        252    1365     393

  Fragments arranged by size: 

                1365     393     252
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      26    1470    2010 
   Size:         26    1444     540

  Fragments arranged by size: 

                1444     540      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1492    2010 
   Size:       1492     518
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     311     324     495     554    1060    1117    1675 
   Size:        232      79      13     171      59     506      57     558

Cuts at:   1675    1690    2010 
   Size:         15     320

  Fragments arranged by size: 

                 558     506     320     232     171      79      59      57
                  15      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     748     877    1072    2010 
   Size:        748     129     195     938

  Fragments arranged by size: 

                 938     748     195     129
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1435    1520    1777    2010 
   Size:       1435      85     257     233

  Fragments arranged by size: 

                1435     257     233      85
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1682    2010 
   Size:       1682     328
 
BsaAI yAC'GTr

Cuts at:      0    1194    2010 
   Size:       1194     816
 
BsaBI GATnn'nnATC

Cuts at:      0    1237    2010 
   Size:       1237     773
 
BsaHI Gr'CG_yC

Cuts at:      0     656     782    1733    2010 
   Size:        656     126     951     277

  Fragments arranged by size: 

                 951     656     277     126
 
BsaWI w'CCGG_w

Cuts at:      0    1266    2010 
   Size:       1266     744
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     475     505    1033    1063    2010 
   Size:        475      30     528      30     947

  Fragments arranged by size: 

                 947     528     475      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     496     982    1934    2010 
   Size:        106     390     486     952      76

  Fragments arranged by size: 

                 952     486     390     106      76
 
BseYI C'CCAG_C

Cuts at:      0     636     649    1080    1328    1385    2010 
   Size:        636      13     431     248      57     625

  Fragments arranged by size: 

                 636     625     431     248      57      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     487    1291    1708    1843    2010 
   Size:        250     237     804     417     135     167

  Fragments arranged by size: 

                 804     417     250     237     167     135
 
BsiHKAI G_wGCw'C

Cuts at:      0     284     378     413     444     569    1166    1450    1492 
   Size:        284      94      35      31     125     597     284      42

Cuts at:   1492    1762    2010 
   Size:        270     248

  Fragments arranged by size: 

                 597     284     284     270     248     125      94      42
                  35      31
 
BsmI GAATG_Cn'

Cuts at:      0    1890    2010 
   Size:       1890     120
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     284     378     413     444     569     770    1166 
   Size:        114     170      94      35      31     125     201     396

Cuts at:   1166    1450    1492    1762    2010 
   Size:        284      42     270     248

  Fragments arranged by size: 

                 396     284     270     248     201     170     125     114
                  94      42      35      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10     509     914    1610    1760    1809    1890    2010 
   Size:         10     499     405     696     150      49      81     120

  Fragments arranged by size: 

                 696     499     405     150     120      81      49      10
            
 
BsrBI CCG'CTC

Cuts at:      0     182     808     888    1401    2010 
   Size:        182     626      80     513     609

  Fragments arranged by size: 

                 626     609     513     182      80
 
BsrFI r'CCGG_y

Cuts at:      0    1214    1634    2010 
   Size:       1214     420     376

  Fragments arranged by size: 

                1214     420     376
 
BssHII G'CGCG_C

Cuts at:      0     948    2010 
   Size:        948    1062
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1878    2010 
   Size:       1878     132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      99    2010 
   Size:         99    1911
 
BstYI r'GATC_y

Cuts at:      0     872    1118    1277    1473    2010 
   Size:        872     246     159     196     537

  Fragments arranged by size: 

                 872     537     246     196     159
 
BtgI C'CryG_G

Cuts at:      0     620     770    1728    2010 
   Size:        620     150     958     282

  Fragments arranged by size: 

                 958     620     282     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     590    2010 
   Size:        590    1420
 
BtsI GCAGTG_nn'

Cuts at:      0     284     422     588     943    2010 
   Size:        284     138     166     355    1067

  Fragments arranged by size: 

                1067     355     284     166     138
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     454    2010 
   Size:        454    1556
 
EaeI y'GGCC_r

Cuts at:      0     403     791    1301    2010 
   Size:        403     388     510     709

  Fragments arranged by size: 

                 709     510     403     388
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     838    1597    1941    2010 
   Size:        838     759     344      69

  Fragments arranged by size: 

                 838     759     344      69
 
EcoICRI GAG'CTC

Cuts at:      0     442     567    1164    1448    1490    2010 
   Size:        442     125     597     284      42     520

  Fragments arranged by size: 

                 597     520     442     284     125      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     311     324     495     554     617    1060    1117 
   Size:        232      79      13     171      59      63     443      57

Cuts at:   1117    1675    1690    2010 
   Size:        558      15     320

  Fragments arranged by size: 

                 558     443     320     232     171      79      63      59
                  57      15      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1803    2010 
   Size:       1803     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     486    1035    1599    2010 
   Size:        111     375     549     564     411

  Fragments arranged by size: 

                 564     549     411     375     111
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102    1731    2010 
   Size:        102    1629     279

  Fragments arranged by size: 

                1629     279     102
 
HaeII r_GCGC'y

Cuts at:      0    1065    1736    1788    2010 
   Size:       1065     671      52     222

  Fragments arranged by size: 

                1065     671     222      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     741     773    2010 
   Size:        741      32    1237

  Fragments arranged by size: 

                1237     741      32
 
KasI G'GCGC_C

Cuts at:      0    1732    2010 
   Size:       1732     278
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    2010 
   Size:        403    1607
 
MscI TGG'CCA

Cuts at:      0     405    2010 
   Size:        405    1605
 
MslI CAynn'nnrTG

Cuts at:      0      97     287     427     664    2010 
   Size:         97     190     140     237    1346

  Fragments arranged by size: 

                1346     237     190     140      97
 
MspA1I CmG'CkG

Cuts at:      0     454     552     772     803     975    1013    1299    1827 
   Size:        454      98     220      31     172      38     286     528

Cuts at:   1827    2010 
   Size:        183

  Fragments arranged by size: 

                 528     454     286     220     183     172      98      38
                  31
 
NaeI GCC'GGC

Cuts at:      0    1216    1636    2010 
   Size:       1216     420     374

  Fragments arranged by size: 

                1216     420     374
 
NarI GG'CG_CC

Cuts at:      0    1733    2010 
   Size:       1733     277
 
NcoI C'CATG_G

Cuts at:      0     620    2010 
   Size:        620    1390
 
NgoMIV G'CCGG_C

Cuts at:      0    1214    1634    2010 
   Size:       1214     420     376

  Fragments arranged by size: 

                1214     420     376
 
NspI r_CATG'y

Cuts at:      0     960    1369    2010 
   Size:        960     409     641

  Fragments arranged by size: 

                 960     641     409
 
PasI CC'CwG_GG

Cuts at:      0       5    2010 
   Size:          5    2005
 
PciI A'CATG_T

Cuts at:      0     956    2010 
   Size:        956    1054
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     636    2010 
   Size:        636    1374
 
PfoI T'CCnGG_A

Cuts at:      0    1094    2010 
   Size:       1094     916
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1033    1065    2010 
   Size:       1033      32     945

  Fragments arranged by size: 

                1033     945      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     486    1599    2010 
   Size:        486    1113     411

  Fragments arranged by size: 

                1113     486     411
 
PshAI GACnn'nnGTC

Cuts at:      0     859    2010 
   Size:        859    1151
 
PspOMI G'GGCC_C

Cuts at:      0     110    2010 
   Size:        110    1900
 
PspXI vC'TCGA_Gb

Cuts at:      0    1520    2010 
   Size:       1520     490
 
PstI C_TGCA'G

Cuts at:      0     132    1226    1298    1865    2010 
   Size:        132    1094      72     567     145

  Fragments arranged by size: 

                1094     567     145     132      72
 
PvuII CAG'CTG

Cuts at:      0     454     552     975    1299    1827    2010 
   Size:        454      98     423     324     528     183

  Fragments arranged by size: 

                 528     454     423     324     183      98
 
SacI G_AGCT'C

Cuts at:      0     444     569    1166    1450    1492    2010 
   Size:        444     125     597     284      42     518

  Fragments arranged by size: 

                 597     518     444     284     125      42
 
SacII CC_GC'GG

Cuts at:      0     773    2010 
   Size:        773    1237
 
ScaI AGT'ACT

Cuts at:      0    1132    2010 
   Size:       1132     878
 
SfcI C'TryA_G

Cuts at:      0     128     522    1222    1294    1861    2010 
   Size:        128     394     700      72     567     149

  Fragments arranged by size: 

                 700     567     394     149     128      72
 
SfoI GGC'GCC

Cuts at:      0    1734    2010 
   Size:       1734     276
 
SmlI C'TyrA_G

Cuts at:      0    1414    1520    1535    1792    2010 
   Size:       1414     106      15     257     218

  Fragments arranged by size: 

                1414     257     218     106      15
 
SphI G_CATG'C

Cuts at:      0    1369    2010 
   Size:       1369     641
 
StuI AGG'CCT

Cuts at:      0    1024    1210    1798    2010 
   Size:       1024     186     588     212

  Fragments arranged by size: 

                1024     588     212     186
 
StyI C'CwwG_G

Cuts at:      0     163     620    2010 
   Size:        163     457    1390

  Fragments arranged by size: 

                1390     457     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1072    2010 
   Size:       1072     938
 
TatI w'GTAC_w

Cuts at:      0    1130    1196    1622    2010 
   Size:       1130      66     426     388

  Fragments arranged by size: 

                1130     426     388      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     190     222    2010 
   Size:        190      32    1788

  Fragments arranged by size: 

                1788     190      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     613    2010 
   Size:        613    1397
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1155    1377    2010 
   Size:       1155     222     633

  Fragments arranged by size: 

                1155     633     222
 
XhoI C'TCGA_G

Cuts at:      0    1520    2010 
   Size:       1520     490
 
XmnI GAAnn'nnTTC

Cuts at:      0      55    1024    1714    2010 
   Size:         55     969     690     296

  Fragments arranged by size: 

                 969     690     296      55
 
ZraI GAC'GTC

Cuts at:      0     657    2010 
   Size:        657    1353
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AlfI     AloI    AlwNI
     ApaI    ApaLI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DrdI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     ScaI     SfcI     SfoI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     ApoI
     AscI     AseI    AsiSI     BaeI    BamHI    BciVI     BclI    BfrBI
     BmtI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     EagI     EarI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PsiI     PsrI
     PvuI    RsrII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
     XbaI     XmaI
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