Restriction map for
pF1KSDA1089
(Circular) (Six-base) MAPSORT of: pF1KSDA1089.seq Check: 3766 from: 1 to: 6187
pF1KSDA1089 6187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2998 2998
Size: 6187
AccI GT'mk_AC
Cuts at: 383 1961 3223 383
Size: 1578 1262 3347
Fragments arranged by size:
3347 1578 1262
Acc65I G'GTAC_C
Cuts at: 2846 3201 2846
Size: 355 5832
AclI AA'CG_TT
Cuts at: 6027 6027
Size: 6187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1955 2047 2608 2830 2950 4010 4442 5191
Size: 1012 92 561 222 120 1060 432 749
Cuts at: 5191 5459 943
Size: 268 1671
Fragments arranged by size:
1671 1060 1012 749 561 432 268 222
120 92
AfeI AGC'GCT
Cuts at: 1861 1861
Size: 6187
AflIII A'CryG_T
Cuts at: 3537 4643 5620 3537
Size: 1106 977 4104
Fragments arranged by size:
4104 1106 977
AgeI A'CCGG_T
Cuts at: 4575 4575
Size: 6187
AleI CACnn'nnGTG
Cuts at: 3119 3119
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 1274 2491 3125 488
Size: 786 1217 634 3550
Fragments arranged by size:
3550 1217 786 634
AlwNI CAG_nnn'CTG
Cuts at: 598 1429 1984 2070 3490 5059 598
Size: 831 555 86 1420 1569 1726
Fragments arranged by size:
1726 1569 1420 831 555 86
ApaI G_GGCC'C
Cuts at: 1798 2832 1798
Size: 1034 5153
ApaLI G'TGCA_C
Cuts at: 1222 4957 1222
Size: 3735 2452
ApoI r'AATT_y
Cuts at: 3189 5540 5796 3189
Size: 2351 256 3580
Fragments arranged by size:
3580 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6187
AvaI C'yCGr_G
Cuts at: 1586 1751 2831 3205 1586
Size: 165 1080 374 4568
Fragments arranged by size:
4568 1080 374 165
BamHI G'GATC_C
Cuts at: 1243 3018 3210 1243
Size: 1775 192 4220
Fragments arranged by size:
4220 1775 192
BanI G'GyrC_C
Cuts at: 781 2846 3201 3615 3863 3898 781
Size: 2065 355 414 248 35 3070
Fragments arranged by size:
3070 2065 414 355 248 35
BanII G_rGCy'C
Cuts at: 164 311 1603 1748 1798 1996 2161 2832 3199
Size: 147 1292 145 50 198 165 671 367
Cuts at: 3199 4229 164
Size: 1030 2122
Fragments arranged by size:
2122 1292 1030 671 367 198 165 147
145 50
BbeI G_GCGC'C
Cuts at: 3619 3867 3619
Size: 248 5939
BbsI GAAGACnn'nnnn_
Cuts at: 2097 2097
Size: 6187
BbvCI CC'TCA_GC
Cuts at: 359 1123 359
Size: 764 5423
BciVI GTATCCnnnnn_n'
Cuts at: 4083 4846 6187 4083
Size: 763 1341 4083
Fragments arranged by size:
4083 1341 763
BclI T'GATC_A
Cuts at: 3705 3705
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 1793 2465 3004 3103 1793
Size: 672 539 99 4877
Fragments arranged by size:
4877 672 539 99
BglII A'GATC_T
Cuts at: 2259 3700 5739 2259
Size: 1441 2039 2707
Fragments arranged by size:
2707 2039 1441
BlpI GC'TnA_GC
Cuts at: 1328 2392 2612 3297 1328
Size: 1064 220 685 4218
Fragments arranged by size:
4218 1064 685 220
Bme1580I G_kGCm'C
Cuts at: 627 784 1226 1798 2832 3810 3903 4961 627
Size: 157 442 572 1034 978 93 1058 1853
Fragments arranged by size:
1853 1058 1034 978 572 442 157 93
BmrI ACTGGGnnnn_n'
Cuts at: 331 1793 1801 2511 2667 3397 3478 3813 5959
Size: 1462 8 710 156 730 81 335 2146
Cuts at: 5959 331
Size: 559
Fragments arranged by size:
2146 1462 730 710 559 335 156 81
8
BmtI G_CTAG'C
Cuts at: 715 1811 715
Size: 1096 5091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1071 1998 2010 4552 1071
Size: 927 12 2542 2706
Fragments arranged by size:
2706 2542 927 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1762 1885 2206 2665 2872 1096
Size: 666 123 321 459 207 4411
Fragments arranged by size:
4411 666 459 321 207 123
Bpu10I CC'TnA_GC
Cuts at: 359 1123 1430 359
Size: 764 307 5116
Fragments arranged by size:
5116 764 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1554 3361 4240 4734 5032 865
Size: 689 1807 879 494 298 2020
Fragments arranged by size:
2020 1807 879 689 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5891 5891
Size: 6187
BsaAI yAC'GTr
Cuts at: 3177 3538 3177
Size: 361 5826
BsaBI GATnn'nnATC
Cuts at: 6 3699 5738 6
Size: 3693 2039 455
Fragments arranged by size:
3693 2039 455
BsaHI Gr'CG_yC
Cuts at: 3616 3864 4568 6064 3616
Size: 248 704 1496 3739
Fragments arranged by size:
3739 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3374 3895 4575 4849 4996 5407 3374
Size: 521 680 274 147 411 4154
Fragments arranged by size:
4154 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 355 385 1093 1123 2845 2875 355
Size: 30 708 30 1722 30 3667
Fragments arranged by size:
3667 1722 708 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 740 1101 1159 1162 1427 1429 1432 1732
Size: 364 361 58 3 265 2 3 300
Cuts at: 1732 1888 2002 2419 3028 376
Size: 156 114 417 609 3535
Fragments arranged by size:
3535 609 417 364 361 300 265 156
114 58 3 3 2
BseYI C'CCAG_C
Cuts at: 777 3067 3610 4368 4947 5443 777
Size: 2290 543 758 579 496 1521
Fragments arranged by size:
2290 1521 758 579 543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1280 316
Size: 964 5223
BsiEI CG_ry'CG
Cuts at: 104 3727 3773 4983 104
Size: 3623 46 1210 1308
Fragments arranged by size:
3623 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 311 442 1226 2161 3199 3977 4167 4961 311
Size: 131 784 935 1038 778 190 794 1537
Fragments arranged by size:
1537 1038 935 794 784 778 190 131
BsmI GAATG_Cn'
Cuts at: 767 1360 767
Size: 593 5594
Bsp1286I G_dGCh'C
Cuts at: 164 311 442 627 784 1226 1603 1748 1798
Size: 147 131 185 157 442 377 145 50
Cuts at: 1798 1996 2161 2832 3199 3810 3903 3977 4167
Size: 198 165 671 367 611 93 74 190
Cuts at: 4167 4229 4961 164
Size: 62 732 1390
Fragments arranged by size:
1390 732 671 611 442 377 367 198
190 185 165 157 147 145 131 93
74 62 50
BspEI T'CCGG_A
Cuts at: 5407 5407
Size: 6187
BspHI T'CATG_A
Cuts at: 2597 5363 2597
Size: 2766 3421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 839 1112 1901 2342 2998 3235 3449 3751 4132
Size: 273 789 441 656 237 214 302 381
Cuts at: 4132 839
Size: 2894
Fragments arranged by size:
2894 789 656 441 381 302 273 237
214
BsrBI CCG'CTC
Cuts at: 50 2343 2919 4477 4531 6017 6182 50
Size: 2293 576 1558 54 1486 165 55
Fragments arranged by size:
2293 1558 1486 576 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 1811 2510 4097 1265
Size: 546 699 1587 3355
Fragments arranged by size:
3355 1587 699 546
BsrFI r'CCGG_y
Cuts at: 4183 4364 4575 4183
Size: 181 211 5795
Fragments arranged by size:
5795 211 181
BssSI C'ACGA_G
Cuts at: 4456 4816 4456
Size: 360 5827
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 2426 3562 1267
Size: 1159 1136 3892
Fragments arranged by size:
3892 1159 1136
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1730 3033 1730
Size: 1303 4884
BstXI CCAn_nnnn'nTGG
Cuts at: 1372 2230 1372
Size: 858 5329
BstYI r'GATC_y
Cuts at: 1 1243 1425 2259 2966 3018 3210 3677 3700
Size: 1242 182 834 707 52 192 467 23
Cuts at: 3700 4035 4281 5295 5381 5393 5739 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1242 1014 834 707 467 449 346 335
246 192 182 86 52 23 12
BstZ17I GTA'TAC
Cuts at: 384 384
Size: 6187
Bsu36I CC'TnA_GG
Cuts at: 289 603 2249 289
Size: 314 1646 4227
Fragments arranged by size:
4227 1646 314
BtgI C'CryG_G
Cuts at: 1037 1037
Size: 6187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 978 2436 4135 4316 4479 978
Size: 1458 1699 181 163 2686
Fragments arranged by size:
2686 1699 1458 181 163
BtsI GCAGTG_nn'
Cuts at: 544 3163 3428 544
Size: 2619 265 3303
Fragments arranged by size:
3303 2619 265
ClaI AT'CG_AT
Cuts at: 2792 2792
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1924 1959 2152 2187 1924
Size: 35 193 35 5924
Fragments arranged by size:
5924 193 35 35
DraI TTT'AAA
Cuts at: 2865 3184 2865
Size: 319 5868
DraIII CAC_nnn'GTG
Cuts at: 339 339
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 2030 3891 4751 2030
Size: 1861 860 3466
Fragments arranged by size:
3466 1861 860
EaeI y'GGCC_r
Cuts at: 1130 1928 2075 2747 2757 3770 3944 4335 4362
Size: 798 147 672 10 1013 174 391 27
Cuts at: 4362 5709 1130
Size: 1347 1608
Fragments arranged by size:
1608 1347 1013 798 672 391 174 147
27 10
EagI C'GGCC_G
Cuts at: 3770 3770
Size: 6187
EarI CTCTTCn'nnn_
Cuts at: 1102 1331 1709 2330 2926 4208 4418 1102
Size: 229 378 621 596 1282 210 2871
Fragments arranged by size:
2871 1282 621 596 378 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1492 2728 4393 4705 4851 5997 1492
Size: 1236 1665 312 146 1146 1682
Fragments arranged by size:
1682 1665 1236 1146 312 146
EcoICRI GAG'CTC
Cuts at: 309 2159 3197 309
Size: 1850 1038 3299
Fragments arranged by size:
3299 1850 1038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1096 1762 1885 1955 2047 2206 2608 2665
Size: 153 666 123 70 92 159 402 57
Cuts at: 2665 2830 2872 2950 4010 4442 5191 5459 943
Size: 165 42 78 1060 432 749 268 1671
Fragments arranged by size:
1671 1060 749 666 432 402 268 165
159 153 123 92 78 70 57 42
EcoO109I rG'GnC_Cy
Cuts at: 136 1795 2067 2712 2828 3082 3324 136
Size: 1659 272 645 116 254 242 2999
Fragments arranged by size:
2999 1659 645 272 254 242 116
EcoRI G'AATT_C
Cuts at: 3189 3189
Size: 6187
EcoRV GAT'ATC
Cuts at: 676 676
Size: 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1881 2104 2281 4204 1881
Size: 223 177 1923 3864
Fragments arranged by size:
3864 1923 223 177
FspI TGC'GCA
Cuts at: 2153 2419 3966 2153
Size: 266 1547 4374
Fragments arranged by size:
4374 1547 266
FspAI rTGC'GCAy
Cuts at: 2419 2419
Size: 6187
HaeII r_GCGC'y
Cuts at: 1863 3619 3867 4891 5870 1863
Size: 1756 248 1024 979 2180
Fragments arranged by size:
2180 1756 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 215 247 621 653 1597 1629 1675 1707 2278
Size: 32 374 32 944 32 46 32 571
Cuts at: 2278 2310 2612 2644 4027 4057 4059 4089 215
Size: 32 302 32 1383 30 2 30 2313
Fragments arranged by size:
2313 1383 944 571 374 302 46 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1311 3224 1311
Size: 1913 4274
HindIII A'AGCT_T
Cuts at: 319 2059 319
Size: 1740 4447
KasI G'GCGC_C
Cuts at: 3615 3863 3615
Size: 248 5939
KpnI G_GTAC'C
Cuts at: 2850 3205 2850
Size: 355 5832
MluI A'CGCG_T
Cuts at: 5620 5620
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1893 1982 2014 2566 4858 5042 1893
Size: 89 32 552 2292 184 3038
Fragments arranged by size:
3038 2292 552 184 89 32
MscI TGG'CCA
Cuts at: 1132 2749 3946 1132
Size: 1617 1197 3373
Fragments arranged by size:
3373 1617 1197
MslI CAynn'nnrTG
Cuts at: 1843 2143 3119 4174 4301 1843
Size: 300 976 1055 127 3729
Fragments arranged by size:
3729 1055 976 300 127
MspA1I CmG'CkG
Cuts at: 528 777 2643 2978 2988 3109 3296 3610 3970
Size: 249 1866 335 10 121 187 314 360
Cuts at: 3970 4985 5230 528
Size: 1015 245 1485
Fragments arranged by size:
1866 1485 1015 360 335 314 249 245
187 121 10
NaeI GCC'GGC
Cuts at: 4366 4366
Size: 6187
NarI GG'CG_CC
Cuts at: 3616 3864 3616
Size: 248 5939
NgoMIV G'CCGG_C
Cuts at: 4364 4364
Size: 6187
NheI G'CTAG_C
Cuts at: 711 1807 711
Size: 1096 5091
NruI TCG'CGA
Cuts at: 4548 4548
Size: 6187
NspI r_CATG'y
Cuts at: 638 3238 3738 4647 638
Size: 2600 500 909 2178
Fragments arranged by size:
2600 2178 909 500
PasI CC'CwG_GG
Cuts at: 2824 3000 2824
Size: 176 6011
PciI A'CATG_T
Cuts at: 4643 4643
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 2494 2494
Size: 6187
PfoI T'CCnGG_A
Cuts at: 1073 1175 2051 2108 2222 1073
Size: 102 876 57 114 5038
Fragments arranged by size:
5038 876 114 102 57
PmeI GTTT'AAAC
Cuts at: 3184 3184
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5326 5358 5326
Size: 32 6155
PpuMI rG'GwC_Cy
Cuts at: 2067 2067
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 559 1622 559
Size: 1063 5124
PspOMI G'GGCC_C
Cuts at: 1794 2828 1794
Size: 1034 5153
PstI C_TGCA'G
Cuts at: 539 1325 2480 2543 2660 3104 3232 3917 539
Size: 786 1155 63 117 444 128 685 2809
Fragments arranged by size:
2809 1155 786 685 444 128 117 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6187
PvuII CAG'CTG
Cuts at: 528 777 2643 2978 2988 3610 3970 528
Size: 249 1866 335 10 622 360 2745
Fragments arranged by size:
2745 1866 622 360 335 249 10
RsrII CG'GwC_CG
Cuts at: 4380 4380
Size: 6187
SacI G_AGCT'C
Cuts at: 311 2161 3199 311
Size: 1850 1038 3299
Fragments arranged by size:
3299 1850 1038
SalI G'TCGA_C
Cuts at: 3222 3222
Size: 6187
SapI GCTCTTCn'nnn_
Cuts at: 2330 4208 4418 2330
Size: 1878 210 4099
Fragments arranged by size:
4099 1878 210
SbfI CC_TGCA'GG
Cuts at: 3104 3232 3104
Size: 128 6059
ScaI AGT'ACT
Cuts at: 802 5704 802
Size: 4902 1285
SexAI A'CCwGG_T
Cuts at: 467 467
Size: 6187
SfcI C'TryA_G
Cuts at: 33 456 535 1018 1321 2476 2539 2656 3100
Size: 423 79 483 303 1155 63 117 444
Cuts at: 3100 3228 3913 4908 5099 33
Size: 128 685 995 191 1121
Fragments arranged by size:
1155 1121 995 685 483 444 423 303
191 128 117 79 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 1793 1793
Size: 6187
SfoI GGC'GCC
Cuts at: 3617 3865 3617
Size: 248 5939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6187
SmaI CCC'GGG
Cuts at: 1588 2833 3207 1588
Size: 1245 374 4568
Fragments arranged by size:
4568 1245 374
SmlI C'TyrA_G
Cuts at: 880 1569 3340 4255 4749 5011 880
Size: 689 1771 915 494 262 2056
Fragments arranged by size:
2056 1771 915 689 494 262
SnaBI TAC'GTA
Cuts at: 3177 3177
Size: 6187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6187
SphI G_CATG'C
Cuts at: 3238 3738 3238
Size: 500 5687
SrfI GCCC'GGGC
Cuts at: 1588 1588
Size: 6187
StuI AGG'CCT
Cuts at: 1941 3050 1941
Size: 1109 5078
StyI C'CwwG_G
Cuts at: 132 1394 2666 2779 3319 3673 132
Size: 1262 1272 113 540 354 2646
Fragments arranged by size:
2646 1272 1262 540 354 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 350 1131 1447 1532 1536 1855 2722 2837 3768
Size: 781 316 85 4 319 867 115 931
Cuts at: 3768 3901 4360 4571 350
Size: 133 459 211 1966
Fragments arranged by size:
1966 931 867 781 459 319 316 211
133 115 85 4
TatI w'GTAC_w
Cuts at: 800 1253 5702 800
Size: 453 4449 1285
Fragments arranged by size:
4449 1285 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 523 555 3036 3068 5704 5736 355
Size: 32 136 32 2481 32 2636 32 806
Fragments arranged by size:
2636 2481 806 136 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1728 3982 1728
Size: 2254 3933
XbaI T'CTAG_A
Cuts at: 3216 3216
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1125 1401 3680 1125
Size: 276 2279 3632
Fragments arranged by size:
3632 2279 276
XmaI C'CCGG_G
Cuts at: 1586 2831 3205 1586
Size: 1245 374 4568
Fragments arranged by size:
4568 1245 374
XmnI GAAnn'nnTTC
Cuts at: 2104 2104
Size: 6187
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BsiWI BsmBI BsrGI BssHII EcoNI FseI
HpaI MfeI NcoI NdeI NotI NsiI PacI PmlI
PsiI PspXI PsrI SacII SanDI SgrAI SspI SwaI
XhoI ZraI