Restriction map for pF1KSDA1074
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BglI        BlnI        BsaAI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoT22I     FseI        
FspI        HpaI        KpnI        KspI        MluI        
MluNI       MroI        MscI        NaeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI           97  GGCGCC               G[GA]^CG[CT]C
AflII         732  CTTAAG               C^TTAAG
Alw44I       1186  GTGCAC               G^TGCAC
ApaLI        1186  GTGCAC               G^TGCAC
AvaI         2594  CTCGAG               C^[CT]CG[GA]G
BanI           97  GGCGCC               G^G[CT][GA]CC
BanII          39  GGGCTC               G[GA]GC[CT]^C
BbeI           97  GGCGCC               GGCGC^C
BfrI          732  CTTAAG               C^TTAAG
BlpI          830  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      830  GCTTAGC              GC^T[ACGT]AGC
BsaHI          97  GGCGCC               G[GA]^CG[CT]C
BsaI          128  GAGACC               GGTCTC(1/5)
BsgI          184  GTGCAG               GTGCAG(16/14)
BsmFI         817  GTCCC                GGGAC(10/14)
BsoBI        2594  CTCGAG               C^[CT]CG[GA]G
BspHI        2706  TCATGA               T^CATGA
BspMI        4243  GCAGGT               ACCTGC(4/8)
BssSI        3243  CACGAG               CACGAG(-5/-1)
BstXI        2913  CCAGTATAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         830  GCTTAGC              GC^T[ACGT]AGC
DraIII       4823  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         731  CCTTAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1475  GATATC               GAT^ATC
HaeII          97  GGCGCC               [GA]GCGC^[CT]
HgaI         2575  GACGC                GACGC(5/10)
Hin1I          97  GGCGCC               G[GA]^CG[CT]C
HincII       4744  GTCAAC               GT[CT]^[GA]AC
HindII       4744  GTCAAC               GT[CT]^[GA]AC
KasI           97  GGCGCC               G^GCGCC
MfeI         3802  CAATTG               C^AATTG
MunI         3802  CAATTG               C^AATTG
NarI           97  GGCGCC               GG^CGCC
NdeI          884  CATATG               CA^TATG
NheI         4734  GCTAGC               G^CTAGC
PaeR7I       2594  CTCGAG               C^TCGAG
RcaI         2706  TCATGA               T^CATGA
SfoI           97  GGCGCC               GGC^GCC
SpeI         4165  ACTAGT               A^CTAGT
SphI         1442  GCATGC               GCATG^C
SspI          511  AATATT               AAT^ATT
StuI         1981  AGGCCT               AGG^CCT
XhoI         2594  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         4256  GTCTAC               GT^[AC][GT]AC
             5040  GTCTAC              
AlwNI        3830  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4015  CAGTCACTG           
Asp700I       961  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2857  GAAGACCTTC          
BcgI         2353  CGAGAACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2586  GCAACTTTCTCG        
BsmI         2604  GAATGC               GAATGC(1/-1)
             2949  GAATGC              
BsrDI        1098  CATTGC               GCAATG(2/0)
             2953  GCAATG              
DraII        2828  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3293  AGGACCT             
EcoO109I     2828  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3293  AGGACCT             
HindIII       828  AAGCTT               A^AGCTT
             3874  AAGCTT              
MspA1I       1562  CAGCTG               C[AC]G^C[GT]G
             2942  CAGCTG              
NcoI          432  CCATGG               C^CATGG
             4149  CCATGG              
NspI         1442  GCATGC               [GA]CATG^[CT]
             2741  GCATGT              
PflMI        1891  CCAGTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2508  CCAAACACTGG         
PpuMI        2828  AGGACCT              [GA]G^G[AT]CC[CT]
             3293  AGGACCT             
PstI         1319  CTGCAG               CTGCA^G
             4241  CTGCAG              
PvuII        1562  CAGCTG               CAG^CTG
             2942  CAGCTG              
Van91I       1891  CCAGTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2508  CCAAACACTGG         
XbaI         3028  TCTAGA               T^CTAGA
             4674  TCTAGA              
XcmI         4751  CCAAACTTCTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4805  CCAGGCCAGTCATGG     
XmnI          961  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2857  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AseI        AsnI        AspHI       
AvaII       BbsI        BbvI        BciVI       BclI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DsaI        EarI        
EcoRI       EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PshBI       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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