Restriction map for pF1KSDA1072
 (Linear) (Six-base) MAPSORT of: KIAA1072.seq  Check: 8923  from: 1  to: 2271



KIAA1072.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     606    2271 
   Size:        511      95    1665

  Fragments arranged by size: 

                1665     511      95
 
AflII C'TTAA_G

Cuts at:      0     981    2271 
   Size:        981    1290
 
AflIII A'CryG_T

Cuts at:      0     747    1032    2271 
   Size:        747     285    1239

  Fragments arranged by size: 

                1239     747     285
 
AleI CACnn'nnGTG

Cuts at:      0    1000    2271 
   Size:       1000    1271
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2142    2174    2271 
   Size:       2142      32      97

  Fragments arranged by size: 

                2142      97      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1258    2271 
   Size:       1258    1013
 
ApaLI G'TGCA_C

Cuts at:      0    1790    2271 
   Size:       1790     481
 
AseI AT'TA_AT

Cuts at:      0    1814    2271 
   Size:       1814     457
 
AvaI C'yCGr_G

Cuts at:      0     133     145     202     265     330    1983    2271 
   Size:        133      12      57      63      65    1653     288

  Fragments arranged by size: 

                1653     288     133      65      63      57      12
 
AvrII C'CTAG_G

Cuts at:      0    1623    2271 
   Size:       1623     648
 
BanI G'GyrC_C

Cuts at:      0    1171    2271 
   Size:       1171    1100
 
BanII G_rGCy'C

Cuts at:      0     146     152     266    1675    2271 
   Size:        146       6     114    1409     596

  Fragments arranged by size: 

                1409     596     146     114       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     612    1292    1706    2271 
   Size:        612     680     414     565

  Fragments arranged by size: 

                 680     612     565     414
 
BbvCI CC'TCA_GC

Cuts at:      0     173     914    1595    2271 
   Size:        173     741     681     676

  Fragments arranged by size: 

                 741     681     676     173
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2172    2271 
   Size:       2172      99
 
BglII A'GATC_T

Cuts at:      0    1716    2244    2271 
   Size:       1716     528      27

  Fragments arranged by size: 

                1716     528      27
 
BlpI GC'TnA_GC

Cuts at:      0    1608    2271 
   Size:       1608     663
 
Bme1580I G_kGCm'C

Cuts at:      0     338    1755    1794    2271 
   Size:        338    1417      39     477

  Fragments arranged by size: 

                1417     477     338      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1749    2271 
   Size:       1749     522
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1958    2271 
   Size:       1958     313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1197    1895    2271 
   Size:         64    1133     698     376

  Fragments arranged by size: 

                1133     698     376      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     173     914    1595    1669    2271 
   Size:        173     741     681      74     602

  Fragments arranged by size: 

                 741     681     602     173      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2069    2271 
   Size:       2069     202
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     672    2271 
   Size:        672    1599
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1142    1172    2271 
   Size:       1142      30    1099

  Fragments arranged by size: 

                1142    1099      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     223     250     283     286     307     310     313 
   Size:         34     189      27      33       3      21       3       3

Cuts at:    313     316     433    2271 
   Size:          3     117    1838

  Fragments arranged by size: 

                1838     189     117      34      33      27      21       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     362     650     683    1429    2271 
   Size:        362     288      33     746     842

  Fragments arranged by size: 

                 842     746     362     288      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     945     979    2271 
   Size:        527     418      34    1292

  Fragments arranged by size: 

                1292     527     418      34
 
BsiEI CG_ry'CG

Cuts at:      0     182     344     394    2271 
   Size:        182     162      50    1877

  Fragments arranged by size: 

                1877     182     162      50
 
BsiHKAI G_wGCw'C

Cuts at:      0    1609    1794    2271 
   Size:       1609     185     477

  Fragments arranged by size: 

                1609     477     185
 
BsmI GAATG_Cn'

Cuts at:      0     755    1934    2271 
   Size:        755    1179     337

  Fragments arranged by size: 

                1179     755     337
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     152     266     338    1609    1675    1755    1794 
   Size:        146       6     114      72    1271      66      80      39

Cuts at:   1794    2271 
   Size:        477

  Fragments arranged by size: 

                1271     477     146     114      80      72      66      39
                   6
 
BspHI T'CATG_A

Cuts at:      0    2226    2271 
   Size:       2226      45
 
BsrBI CCG'CTC

Cuts at:      0     384     951    2271 
   Size:        384     567    1320

  Fragments arranged by size: 

                1320     567     384
 
BsrDI GCAATG_nn'

Cuts at:      0    1852    2271 
   Size:       1852     419
 
BsrFI r'CCGG_y

Cuts at:      0       4      32    2271 
   Size:          4      28    2239

  Fragments arranged by size: 

                2239      28       4
 
BssSI C'ACGA_G

Cuts at:      0     634    1421    2271 
   Size:        634     787     850

  Fragments arranged by size: 

                 850     787     634
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1258    2271 
   Size:       1258    1013
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1002    1272    2271 
   Size:       1002     270     999

  Fragments arranged by size: 

                1002     999     270
 
BstYI r'GATC_y

Cuts at:      0    1716    2244    2271 
   Size:       1716     528      27

  Fragments arranged by size: 

                1716     528      27
 
Bsu36I CC'TnA_GG

Cuts at:      0      17    1468    2271 
   Size:         17    1451     803

  Fragments arranged by size: 

                1451     803      17
 
BtgI C'CryG_G

Cuts at:      0     218     828    2167    2271 
   Size:        218     610    1339     104

  Fragments arranged by size: 

                1339     610     218     104
 
BtsI GCAGTG_nn'

Cuts at:      0     821    1349    1886    2271 
   Size:        821     528     537     385

  Fragments arranged by size: 

                 821     537     528     385
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     748    2271 
   Size:        748    1523
 
EaeI y'GGCC_r

Cuts at:      0       2     179     215     341     825    2271 
   Size:          2     177      36     126     484    1446

  Fragments arranged by size: 

                1446     484     177     126      36       2
 
EagI C'GGCC_G

Cuts at:      0     179     341    2271 
   Size:        179     162    1930

  Fragments arranged by size: 

                1930     179     162
 
EarI CTCTTCn'nnn_

Cuts at:      0     781    2134    2271 
   Size:        781    1353     137

  Fragments arranged by size: 

                1353     781     137
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     874    2271 
   Size:        874    1397
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     511     606    1197    1895    2271 
   Size:         64     447      95     591     698     376

  Fragments arranged by size: 

                 698     591     447     376      95      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     918     929    1143    1679    2271 
   Size:        918      11     214     536     592

  Fragments arranged by size: 

                 918     592     536     214      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1825    2271 
   Size:       1825     446
 
FspI TGC'GCA

Cuts at:      0     727    2271 
   Size:        727    1544
 
HaeII r_GCGC'y

Cuts at:      0      39    2271 
   Size:         39    2232
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1246    1278    1417    1449    1767    1799    1822    1854 
   Size:       1246      32     139      32     318      32      23      32

Cuts at:   1854    2271 
   Size:        417

  Fragments arranged by size: 

                1246     417     318     139      32      32      32      32
                  23
 
HincII GTy'rAC

Cuts at:      0     876    2271 
   Size:        876    1395
 
HindIII A'AGCT_T

Cuts at:      0     803    2271 
   Size:        803    1468
 
HpaI GTT'AAC

Cuts at:      0     876    2271 
   Size:        876    1395
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1382    1940    2164    2271 
   Size:       1382     558     224     107

  Fragments arranged by size: 

                1382     558     224     107
 
MscI TGG'CCA

Cuts at:      0     827    2271 
   Size:        827    1444
 
MslI CAynn'nnrTG

Cuts at:      0    1000    2271 
   Size:       1000    1271
 
MspA1I CmG'CkG

Cuts at:      0     366     396     650    2271 
   Size:        366      30     254    1621

  Fragments arranged by size: 

                1621     366     254      30
 
NaeI GCC'GGC

Cuts at:      0       6    2271 
   Size:          6    2265
 
NcoI C'CATG_G

Cuts at:      0     828    2167    2271 
   Size:        828    1339     104

  Fragments arranged by size: 

                1339     828     104
 
NgoMIV G'CCGG_C

Cuts at:      0       4    2271 
   Size:          4    2267
 
NspI r_CATG'y

Cuts at:      0     726     751    1029    1036    2271 
   Size:        726      25     278       7    1235

  Fragments arranged by size: 

                1235     726     278      25       7
 
PasI CC'CwG_GG

Cuts at:      0    1675    2271 
   Size:       1675     596
 
PciI A'CATG_T

Cuts at:      0     747    1032    2271 
   Size:        747     285    1239

  Fragments arranged by size: 

                1239     747     285
 
PfoI T'CCnGG_A

Cuts at:      0      41    2271 
   Size:         41    2230
 
PpuMI rG'GwC_Cy

Cuts at:      0    1679    2271 
   Size:       1679     592
 
PshAI GACnn'nnGTC

Cuts at:      0     396    2271 
   Size:        396    1875
 
PstI C_TGCA'G

Cuts at:      0     455     649    2271 
   Size:        455     194    1622

  Fragments arranged by size: 

                1622     455     194
 
PvuII CAG'CTG

Cuts at:      0     366     650    2271 
   Size:        366     284    1621

  Fragments arranged by size: 

                1621     366     284
 
RsrII CG'GwC_CG

Cuts at:      0      70    2271 
   Size:         70    2201
 
SanDI GG'GwC_CC

Cuts at:      0    1679    2271 
   Size:       1679     592
 
SapI GCTCTTCn'nnn_

Cuts at:      0     781    2271 
   Size:        781    1490
 
SfcI C'TryA_G

Cuts at:      0     451     645    2271 
   Size:        451     194    1626

  Fragments arranged by size: 

                1626     451     194
 
SmaI CCC'GGG

Cuts at:      0     204     332    1985    2271 
   Size:        204     128    1653     286

  Fragments arranged by size: 

                1653     286     204     128
 
SmlI C'TyrA_G

Cuts at:      0     981    2084    2271 
   Size:        981    1103     187

  Fragments arranged by size: 

                1103     981     187
 
StyI C'CwwG_G

Cuts at:      0     828    1146    1588    1623    2167    2251    2271 
   Size:        828     318     442      35     544      84      20

  Fragments arranged by size: 

                 828     544     442     318      84      35      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     642    1032    1036    1297    2271 
   Size:        642     390       4     261     974

  Fragments arranged by size: 

                 974     642     390     261       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     536     568    2271 
   Size:        536      32    1703

  Fragments arranged by size: 

                1703     536      32
 
XbaI T'CTAG_A

Cuts at:      0    1999    2271 
   Size:       1999     272
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2249    2271 
   Size:       2249      22
 
XmaI C'CCGG_G

Cuts at:      0     202     330    1983    2271 
   Size:        202     128    1653     288

  Fragments arranged by size: 

                1653     288     202     128
 
XmnI GAAnn'nnTTC

Cuts at:      0     524     661    2271 
   Size:        524     137    1610

  Fragments arranged by size: 

                1610     524     137
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI     AloI    AlwNI    ApaLI     AseI
     AvaI    AvrII     BanI    BanII     BbsI    BbvCI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI     PciI     PfoI    PpuMI    PshAI     PstI    PvuII
    RsrII    SanDI     SapI     SfcI     SmaI     SmlI     StyI    TaqII
     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AlfI     ApaI     ApoI     AscI    AsiSI     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BspMI    BsrGI   BssHII
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI     SacI    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XhoI     ZraI
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