Restriction map for pF1KSDA1029
 (Linear) (Six-base) MAPSORT of: KIAA1029.seq  Check: 7882  from: 1  to: 2709



KIAA1029.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     662    2709 
   Size:        662    2047
 
Acc65I G'GTAC_C

Cuts at:      0      69    1344    2709 
   Size:         69    1275    1365

  Fragments arranged by size: 

                1365    1275      69
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1081    2709 
   Size:       1081    1628
 
AfeI AGC'GCT

Cuts at:      0    2203    2709 
   Size:       2203     506
 
AflIII A'CryG_T

Cuts at:      0    2576    2709 
   Size:       2576     133
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1047    2709 
   Size:       1047    1662
 
AleI CACnn'nnGTG

Cuts at:      0     601    1906    2709 
   Size:        601    1305     803

  Fragments arranged by size: 

                1305     803     601
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1919    2709 
   Size:       1919     790
 
AlwNI CAG_nnn'CTG

Cuts at:      0     324     388    2709 
   Size:        324      64    2321

  Fragments arranged by size: 

                2321     324      64
 
ApaI G_GGCC'C

Cuts at:      0    2119    2709 
   Size:       2119     590
 
ApoI r'AATT_y

Cuts at:      0     348    2709 
   Size:        348    2361
 
AvaI C'yCGr_G

Cuts at:      0    1576    1967    2112    2709 
   Size:       1576     391     145     597

  Fragments arranged by size: 

                1576     597     391     145
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1336    1369    2709 
   Size:       1336      33    1340

  Fragments arranged by size: 

                1340    1336      33
 
BamHI G'GATC_C

Cuts at:      0     731    2709 
   Size:        731    1978
 
BanI G'GyrC_C

Cuts at:      0      35      69     432     738     748    1344    1794    2326 
   Size:         35      34     363     306      10     596     450     532

Cuts at:   2326    2361    2709 
   Size:         35     348

  Fragments arranged by size: 

                 596     532     450     363     348     306      35      35
                  34      10
 
BanII G_rGCy'C

Cuts at:      0     772     844    1259    1448    1676    1818    1915    2113 
   Size:        772      72     415     189     228     142      97     198

Cuts at:   2113    2119    2479    2521    2709 
   Size:          6     360      42     188

  Fragments arranged by size: 

                 772     415     360     228     198     189     188     142
                  97      72      42       6
 
BbeI G_GCGC'C

Cuts at:      0     742    2330    2365    2709 
   Size:        742    1588      35     344

  Fragments arranged by size: 

                1588     742     344      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1043    1484    2709 
   Size:       1043     441    1225

  Fragments arranged by size: 

                1225    1043     441
 
BbvCI CC'TCA_GC

Cuts at:      0     179     440    2709 
   Size:        179     261    2269

  Fragments arranged by size: 

                2269     261     179
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1040    2709 
   Size:       1040    1669
 
BglI GCCn_nnn'nGGC

Cuts at:      0     988    1579    1978    2099    2538    2709 
   Size:        988     591     399     121     439     171

  Fragments arranged by size: 

                 988     591     439     399     171     121
 
BlpI GC'TnA_GC

Cuts at:      0    1442    2709 
   Size:       1442    1267
 
Bme1580I G_kGCm'C

Cuts at:      0     751    1799    2119    2709 
   Size:        751    1048     320     590

  Fragments arranged by size: 

                1048     751     590     320
 
BmgBI CAC'GTC

Cuts at:      0    2577    2709 
   Size:       2577     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     221     818    1276    2709 
   Size:        221     597     458    1433

  Fragments arranged by size: 

                1433     597     458     221
 
BmtI G_CTAG'C

Cuts at:      0      26    2709 
   Size:         26    2683
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27    1025    1374    1462    2709 
   Size:         27     998     349      88    1247

  Fragments arranged by size: 

                1247     998     349      88      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     158     470     495     522    1120    1935    2597 
   Size:         61      97     312      25      27     598     815     662

Cuts at:   2597    2709 
   Size:        112

  Fragments arranged by size: 

                 815     662     598     312     112      97      61      27
                  25
 
Bpu10I CC'TnA_GC

Cuts at:      0     179     440    2024    2709 
   Size:        179     261    1584     685

  Fragments arranged by size: 

                1584     685     261     179
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1351    2653    2709 
   Size:       1351    1302      56

  Fragments arranged by size: 

                1351    1302      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     961    1010    1752    1967    2221    2709 
   Size:        961      49     742     215     254     488

  Fragments arranged by size: 

                 961     742     488     254     215      49
 
BsaBI GATnn'nnATC

Cuts at:      0     287    2709 
   Size:        287    2422
 
BsaHI Gr'CG_yC

Cuts at:      0     739     927    2065    2327    2362    2709 
   Size:        739     188    1138     262      35     347

  Fragments arranged by size: 

                1138     739     347     262     188      35
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31      73     457     780     998    1117    1264    1267 
   Size:         31      42     384     323     218     119     147       3

Cuts at:   1267    1351    1845    2709 
   Size:         84     494     864

  Fragments arranged by size: 

                 864     494     384     323     218     147     119      84
                  42      31       3
 
BseYI C'CCAG_C

Cuts at:      0     149     192     400     532     546     815    1033    1605 
   Size:        149      43     208     132      14     269     218     572

Cuts at:   1605    1777    2086    2102    2245    2709 
   Size:        172     309      16     143     464

  Fragments arranged by size: 

                 572     464     309     269     218     208     172     149
                 143     132      43      16      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     932    2487    2685    2709 
   Size:        109     823    1555     198      24

  Fragments arranged by size: 

                1555     823     198     109      24
 
BsiEI CG_ry'CG

Cuts at:      0    2320    2709 
   Size:       2320     389
 
BsiHKAI G_wGCw'C

Cuts at:      0    1448    2709 
   Size:       1448    1261
 
BsmI GAATG_Cn'

Cuts at:      0     102    1094    2709 
   Size:        102     992    1615

  Fragments arranged by size: 

                1615     992     102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     777     918    2709 
   Size:        777     141    1791

  Fragments arranged by size: 

                1791     777     141
 
Bsp1286I G_dGCh'C

Cuts at:      0     751     772     844    1259    1448    1676    1799    1818 
   Size:        751      21      72     415     189     228     123      19

Cuts at:   1818    1915    2113    2119    2479    2521    2709 
   Size:         97     198       6     360      42     188

  Fragments arranged by size: 

                 751     415     360     228     198     189     188     123
                  97      72      42      21      19       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1652    1811    2709 
   Size:       1652     159     898

  Fragments arranged by size: 

                1652     898     159
 
BsrBI CCG'CTC

Cuts at:      0    2558    2709 
   Size:       2558     151
 
BsrFI r'CCGG_y

Cuts at:      0     162     300     435    2488    2709 
   Size:        162     138     135    2053     221

  Fragments arranged by size: 

                2053     221     162     138     135
 
BssSI C'ACGA_G

Cuts at:      0    1307    2190    2709 
   Size:       1307     883     519

  Fragments arranged by size: 

                1307     883     519
 
BstEII G'GTnAC_C

Cuts at:      0    1164    2709 
   Size:       1164    1545
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     759    1785    2709 
   Size:        759    1026     924

  Fragments arranged by size: 

                1026     924     759
 
BstYI r'GATC_y

Cuts at:      0     731    1188    2709 
   Size:        731     457    1521

  Fragments arranged by size: 

                1521     731     457
 
BtgI C'CryG_G

Cuts at:      0     878    1325    1635    2119    2314    2338    2470    2564 
   Size:        878     447     310     484     195      24     132      94

Cuts at:   2564    2643    2709 
   Size:         79      66

  Fragments arranged by size: 

                 878     484     447     310     195     132      94      79
                  66      24
 
BtsI GCAGTG_nn'

Cuts at:      0     103     944    1901    2709 
   Size:        103     841     957     808

  Fragments arranged by size: 

                 957     841     808     103
 
DraIII CAC_nnn'GTG

Cuts at:      0     410    1309    2709 
   Size:        410     899    1400

  Fragments arranged by size: 

                1400     899     410
 
EaeI y'GGCC_r

Cuts at:      0      50     686     716     847    1881    2709 
   Size:         50     636      30     131    1034     828

  Fragments arranged by size: 

                1034     828     636     131      50      30
 
EarI CTCTTCn'nnn_

Cuts at:      0      59    2709 
   Size:         59    2650
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     260    1348    2709 
   Size:        260    1088    1361

  Fragments arranged by size: 

                1361    1088     260
 
EcoICRI GAG'CTC

Cuts at:      0    1446    2709 
   Size:       1446    1263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     158     470     495     522    1081    1120    1935 
   Size:         61      97     312      25      27     559      39     815

Cuts at:   1935    2597    2709 
   Size:        662     112

  Fragments arranged by size: 

                 815     662     559     312     112      97      61      39
                  27      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      43     999    1420    1807    2709 
   Size:         43     956     421     387     902

  Fragments arranged by size: 

                 956     902     421     387      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     385     542     804    1580    1659    1980    1988    2540 
   Size:        385     157     262     776      79     321       8     552

Cuts at:   2540    2709 
   Size:        169

  Fragments arranged by size: 

                 776     552     385     321     262     169     157      79
                   8
 
EcoRI G'AATT_C

Cuts at:      0     348    2709 
   Size:        348    2361
 
HaeII r_GCGC'y

Cuts at:      0     742    2205    2266    2309    2330    2365    2709 
   Size:        742    1463      61      43      21      35     344

  Fragments arranged by size: 

                1463     742     344      61      43      35      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     463     495     547     579     958     990    1033    1065 
   Size:        463      32      52      32     379      32      43      32

Cuts at:   1065    1158    1190    2007    2039    2709 
   Size:         93      32     817      32     670

  Fragments arranged by size: 

                 817     670     463     379      93      52      43      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     207     708    2709 
   Size:        207     501    2001

  Fragments arranged by size: 

                2001     501     207
 
KasI G'GCGC_C

Cuts at:      0     738    2326    2361    2709 
   Size:        738    1588      35     348

  Fragments arranged by size: 

                1588     738     348      35
 
KpnI G_GTAC'C

Cuts at:      0      73    1348    2709 
   Size:         73    1275    1361

  Fragments arranged by size: 

                1361    1275      73
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     366    1064    1307    2597    2709 
   Size:        366     698     243    1290     112

  Fragments arranged by size: 

                1290     698     366     243     112
 
MscI TGG'CCA

Cuts at:      0      52     688    2709 
   Size:         52     636    2021

  Fragments arranged by size: 

                2021     636      52
 
MslI CAynn'nnrTG

Cuts at:      0     601    1906    2709 
   Size:        601    1305     803

  Fragments arranged by size: 

                1305     803     601
 
MspA1I CmG'CkG

Cuts at:      0     178     196     640    1204    1327    1520    1698    2163 
   Size:        178      18     444     564     123     193     178     465

Cuts at:   2163    2217    2256    2316    2340    2472    2523    2645    2709 
   Size:         54      39      60      24     132      51     122      64

  Fragments arranged by size: 

                 564     465     444     193     178     178     132     123
                 122      64      60      54      51      39      24      18
            
 
NarI GG'CG_CC

Cuts at:      0     739    2327    2362    2709 
   Size:        739    1588      35     347

  Fragments arranged by size: 

                1588     739     347      35
 
NcoI C'CATG_G

Cuts at:      0     878    1635    2709 
   Size:        878     757    1074

  Fragments arranged by size: 

                1074     878     757
 
NheI G'CTAG_C

Cuts at:      0      22    2709 
   Size:         22    2687
 
NspI r_CATG'y

Cuts at:      0    2406    2709 
   Size:       2406     303
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     376    1958    2709 
   Size:        376    1582     751

  Fragments arranged by size: 

                1582     751     376
 
PfoI T'CCnGG_A

Cuts at:      0    1552    2709 
   Size:       1552    1157
 
PpuMI rG'GwC_Cy

Cuts at:      0    1659    2709 
   Size:       1659    1050
 
PspOMI G'GGCC_C

Cuts at:      0    2115    2709 
   Size:       2115     594
 
PstI C_TGCA'G

Cuts at:      0     201    1898    2261    2709 
   Size:        201    1697     363     448

  Fragments arranged by size: 

                1697     448     363     201
 
PvuII CAG'CTG

Cuts at:      0     178     196     640    1698    2256    2709 
   Size:        178      18     444    1058     558     453

  Fragments arranged by size: 

                1058     558     453     444     178      18
 
SacI G_AGCT'C

Cuts at:      0    1448    2709 
   Size:       1448    1261
 
SacII CC_GC'GG

Cuts at:      0    1328    2317    2341    2473    2646    2709 
   Size:       1328     989      24     132     173      63

  Fragments arranged by size: 

                1328     989     173     132      63      24
 
SexAI A'CCwGG_T

Cuts at:      0    1790    2709 
   Size:       1790     919
 
SfcI C'TryA_G

Cuts at:      0     197     663    1894    2257    2709 
   Size:        197     466    1231     363     452

  Fragments arranged by size: 

                1231     466     452     363     197
 
SfoI GGC'GCC

Cuts at:      0     740    2328    2363    2709 
   Size:        740    1588      35     346

  Fragments arranged by size: 

                1588     740     346      35
 
SmaI CCC'GGG

Cuts at:      0    1578    2114    2709 
   Size:       1578     536     595

  Fragments arranged by size: 

                1578     595     536
 
SmlI C'TyrA_G

Cuts at:      0    1366    2668    2709 
   Size:       1366    1302      41

  Fragments arranged by size: 

                1366    1302      41
 
SphI G_CATG'C

Cuts at:      0    2406    2709 
   Size:       2406     303
 
SrfI GCCC'GGGC

Cuts at:      0    2114    2709 
   Size:       2114     595
 
StuI AGG'CCT

Cuts at:      0     723    1279    1426    2695    2709 
   Size:        723     556     147    1269      14

  Fragments arranged by size: 

                1269     723     556     147      14
 
StyI C'CwwG_G

Cuts at:      0     656     878     932    1160    1635    1742    1784    1808 
   Size:        656     222      54     228     475     107      42      24

Cuts at:   1808    2709 
   Size:        901

  Fragments arranged by size: 

                 901     656     475     228     222     107      54      42
                  24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     987    2094    2245    2709 
   Size:        987    1107     151     464

  Fragments arranged by size: 

                1107     987     464     151
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     850     882    1364    1396    2709 
   Size:        850      32     482      32    1313

  Fragments arranged by size: 

                1313     850     482      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     972    2709 
   Size:        972    1737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     600     984    2709 
   Size:        600     384    1725

  Fragments arranged by size: 

                1725     600     384
 
XmaI C'CCGG_G

Cuts at:      0    1576    2112    2709 
   Size:       1576     536     597

  Fragments arranged by size: 

                1576     597     536
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstEII    BstXI    BstYI
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NheI     NspI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BcgI     BclI    BfrBI    BglII    BsaAI
    BsaWI    BsaXI    BsiWI    BspEI    BspHI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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