Restriction map for pF1KSDA0976
 (Circular) (Six-base) MAPSORT of: pF1KSDA0976.seq  Check: 4673  from: 1  to: 4231



pF1KSDA0976  4231 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:    766    1267     766 
   Size:        501    3730
 
Acc65I G'GTAC_C

Cuts at:   1245    1245 
   Size:       4231
 
AclI AA'CG_TT

Cuts at:   4071    4071 
   Size:       4231
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2054    2486    3235    3503    2054 
   Size:        432     749     268    2782

  Fragments arranged by size: 

                2782     749     432     268
 
AflIII A'CryG_T

Cuts at:    383    1581    2687    3664     383 
   Size:       1198    1106     977     950

  Fragments arranged by size: 

                1198    1106     977     950
 
AgeI A'CCGG_T

Cuts at:   2619    2619 
   Size:       4231
 
AlwNI CAG_nnn'CTG

Cuts at:   1534    3103    1534 
   Size:       1569    2662
 
ApaLI G'TGCA_C

Cuts at:   3001    3001 
   Size:       4231
 
ApoI r'AATT_y

Cuts at:   1233    3584    3840    1233 
   Size:       2351     256    1624

  Fragments arranged by size: 

                2351    1624     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4231
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4231
 
AvaI C'yCGr_G

Cuts at:   1249    1249 
   Size:       4231
 
BamHI G'GATC_C

Cuts at:    690    1254     690 
   Size:        564    3667
 
BanI G'GyrC_C

Cuts at:   1245    1659    1907    1942    1245 
   Size:        414     248      35    3534

  Fragments arranged by size: 

                3534     414     248      35
 
BanII G_rGCy'C

Cuts at:   1243    2273    1243 
   Size:       1030    3201
 
BbeI G_GCGC'C

Cuts at:   1663    1911    1663 
   Size:        248    3983
 
BbsI GAAGACnn'nnnn_

Cuts at:    465     465 
   Size:       4231
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    995    1029     995 
   Size:         34    4197
 
BciVI GTATCCnnnnn_n'

Cuts at:    512     861    1198    2127    2890    4231     512 
   Size:        349     337     929     763    1341     512

  Fragments arranged by size: 

                1341     929     763     512     349     337
 
BclI T'GATC_A

Cuts at:   1749    1749 
   Size:       4231
 
BglII A'GATC_T

Cuts at:   1744    3783    1744 
   Size:       2039    2192
 
BlpI GC'TnA_GC

Cuts at:   1341    1341 
   Size:       4231
 
Bme1580I G_kGCm'C

Cuts at:   1854    1947    3005    1854 
   Size:         93    1058    3080

  Fragments arranged by size: 

                3080    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1187    1441    1522    1857    4003    1187 
   Size:        254      81     335    2146    1415

  Fragments arranged by size: 

                2146    1415     335     254      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2596    2596 
   Size:       4231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    499     631     499 
   Size:        132    4099
 
Bpu10I CC'TnA_GC

Cuts at:    416     434     416 
   Size:         18    4213
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1405    2284    2778    3076    1405 
   Size:        879     494     298    2560

  Fragments arranged by size: 

                2560     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    338    3935     338 
   Size:       3597     634
 
BsaAI yAC'GTr

Cuts at:   1221    1582    1221 
   Size:        361    3870
 
BsaBI GATnn'nnATC

Cuts at:      6    1743    3782       6 
   Size:       1737    2039     455

  Fragments arranged by size: 

                2039    1737     455
 
BsaHI Gr'CG_yC

Cuts at:    591    1660    1908    2612    4108     591 
   Size:       1069     248     704    1496     714

  Fragments arranged by size: 

                1496    1069     714     704     248
 
BsaWI w'CCGG_w

Cuts at:    327    1418    1939    2619    2893    3040    3451     327 
   Size:       1091     521     680     274     147     411    1107

  Fragments arranged by size: 

                1107    1091     680     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1654    2412    2991    3487    1654 
   Size:        758     579     496    2398

  Fragments arranged by size: 

                2398     758     579     496
 
BsiEI CG_ry'CG

Cuts at:    104    1771    1817    3027     104 
   Size:       1667      46    1210    1308

  Fragments arranged by size: 

                1667    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:   1082    1243    2021    2211    3005    1082 
   Size:        161     778     190     794    2308

  Fragments arranged by size: 

                2308     794     778     190     161
 
BsmBI CGTCTCn'nnnn_

Cuts at:    349     349 
   Size:       4231
 
Bsp1286I G_dGCh'C

Cuts at:   1082    1243    1854    1947    2021    2211    2273    3005    1082 
   Size:        161     611      93      74     190      62     732    2308

  Fragments arranged by size: 

                2308     732     611     190     161      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:    327    3451     327 
   Size:       3124    1107
 
BspHI T'CATG_A

Cuts at:   3407    3407 
   Size:       4231
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1279    1493    1795    2176    1279 
   Size:        214     302     381    3334

  Fragments arranged by size: 

                3334     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2521    2575    4061    4226      50 
   Size:       2471      54    1486     165      55

  Fragments arranged by size: 

                2471    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    374    2141     374 
   Size:       1767    2464
 
BsrFI r'CCGG_y

Cuts at:   2227    2408    2619    2227 
   Size:        181     211    3839

  Fragments arranged by size: 

                3839     211     181
 
BssSI C'ACGA_G

Cuts at:   2500    2860    2500 
   Size:        360    3871
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1606    1606 
   Size:       4231
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4231
 
BstYI r'GATC_y

Cuts at:      1     690    1254    1721    1744    2079    2325    3339    3425 
   Size:        689     564     467      23     335     246    1014      86

Cuts at:   3425    3437    3783       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1014     689     564     467     449     346     335     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    767     767 
   Size:       4231
 
Bsu36I CC'TnA_GG

Cuts at:    910     910 
   Size:       4231
 
BtgI C'CryG_G

Cuts at:    287     287 
   Size:       4231
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2179    2360    2523    2179 
   Size:        181     163    3887

  Fragments arranged by size: 

                3887     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1172    1472    1172 
   Size:        300    3931
 
DraI TTT'AAA

Cuts at:   1228    1228 
   Size:       4231
 
DrdI GACnn_nn'nnGTC

Cuts at:   1139    1935    2795    1139 
   Size:        796     860    2575

  Fragments arranged by size: 

                2575     860     796
 
EaeI y'GGCC_r

Cuts at:   1814    1988    2379    2406    3753    1814 
   Size:        174     391      27    1347    2292

  Fragments arranged by size: 

                2292    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   1814    1814 
   Size:       4231
 
EarI CTCTTCn'nnn_

Cuts at:    743    2252    2462     743 
   Size:       1509     210    2512

  Fragments arranged by size: 

                2512    1509     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2437    2749    2895    4041    2437 
   Size:        312     146    1146    2627

  Fragments arranged by size: 

                2627    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   1241    1241 
   Size:       4231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    499     631    2054    2486    3235    3503     499 
   Size:        132    1423     432     749     268    1227

  Fragments arranged by size: 

                1423    1227     749     432     268     132
 
EcoO109I rG'GnC_Cy

Cuts at:   1368    1368 
   Size:       4231
 
EcoRI G'AATT_C

Cuts at:   1233    1233 
   Size:       4231
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2248    2248 
   Size:       4231
 
FspI TGC'GCA

Cuts at:   2010    2010 
   Size:       4231
 
HaeII r_GCGC'y

Cuts at:   1663    1911    2935    3914    1663 
   Size:        248    1024     979    1980

  Fragments arranged by size: 

                1980    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    288     320     682     714    2071    2101    2103    2133     288 
   Size:         32     362      32    1357      30       2      30    2386

  Fragments arranged by size: 

                2386    1357     362      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    522    1268     522 
   Size:        746    3485
 
HpaI GTT'AAC

Cuts at:    522     522 
   Size:       4231
 
KasI G'GCGC_C

Cuts at:   1659    1907    1659 
   Size:        248    3983
 
KpnI G_GTAC'C

Cuts at:   1249    1249 
   Size:       4231
 
MluI A'CGCG_T

Cuts at:   3664    3664 
   Size:       4231
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2902    3086    2902 
   Size:        184    4047
 
MscI TGG'CCA

Cuts at:   1990    1990 
   Size:       4231
 
MslI CAynn'nnrTG

Cuts at:    292    1000    1071    2218    2345     292 
   Size:        708      71    1147     127    2178

  Fragments arranged by size: 

                2178    1147     708     127      71
 
MspA1I CmG'CkG

Cuts at:    864    1340    1654    2014    3029    3274     864 
   Size:        476     314     360    1015     245    1821

  Fragments arranged by size: 

                1821    1015     476     360     314     245
 
NaeI GCC'GGC

Cuts at:   2410    2410 
   Size:       4231
 
NarI GG'CG_CC

Cuts at:   1660    1908    1660 
   Size:        248    3983
 
NgoMIV G'CCGG_C

Cuts at:   2408    2408 
   Size:       4231
 
NruI TCG'CGA

Cuts at:   2592    2592 
   Size:       4231
 
NspI r_CATG'y

Cuts at:    387    1070    1282    1782    2691     387 
   Size:        683     212     500     909    1927

  Fragments arranged by size: 

                1927     909     683     500     212
 
PciI A'CATG_T

Cuts at:    383    2687     383 
   Size:       2304    1927
 
PflMI CCAn_nnn'nTGG

Cuts at:    476     937    1102     476 
   Size:        461     165    3605

  Fragments arranged by size: 

                3605     461     165
 
PfoI T'CCnGG_A

Cuts at:    608     608 
   Size:       4231
 
PmeI GTTT'AAAC

Cuts at:   1228    1228 
   Size:       4231
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3370    3402    3370 
   Size:         32    4199
 
PstI C_TGCA'G

Cuts at:   1276    1961    1276 
   Size:        685    3546
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4231
 
PvuII CAG'CTG

Cuts at:    864    1654    2014     864 
   Size:        790     360    3081

  Fragments arranged by size: 

                3081     790     360
 
RsrII CG'GwC_CG

Cuts at:   2424    2424 
   Size:       4231
 
SacI G_AGCT'C

Cuts at:   1243    1243 
   Size:       4231
 
SalI G'TCGA_C

Cuts at:   1266    1266 
   Size:       4231
 
SapI GCTCTTCn'nnn_

Cuts at:   2252    2462    2252 
   Size:        210    4021
 
SbfI CC_TGCA'GG

Cuts at:   1276    1276 
   Size:       4231
 
ScaI AGT'ACT

Cuts at:    754    3748     754 
   Size:       2994    1237
 
SfcI C'TryA_G

Cuts at:     33    1088    1272    1957    2952    3143      33 
   Size:       1055     184     685     995     191    1121

  Fragments arranged by size: 

                1121    1055     995     685     191     184
 
SfoI GGC'GCC

Cuts at:   1661    1909    1661 
   Size:        248    3983
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4231
 
SmaI CCC'GGG

Cuts at:   1251    1251 
   Size:       4231
 
SmlI C'TyrA_G

Cuts at:   1384    2299    2793    3055    1384 
   Size:        915     494     262    2560

  Fragments arranged by size: 

                2560     915     494     262
 
SnaBI TAC'GTA

Cuts at:   1221    1221 
   Size:       4231
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4231
 
SphI G_CATG'C

Cuts at:   1282    1782    1282 
   Size:        500    3731
 
SspI AAT'ATT

Cuts at:    947     947 
   Size:       4231
 
StyI C'CwwG_G

Cuts at:   1135    1363    1717    1135 
   Size:        228     354    3649

  Fragments arranged by size: 

                3649     354     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1812    1945    2404    2615    1812 
   Size:        133     459     211    3428

  Fragments arranged by size: 

                3428     459     211     133
 
TatI w'GTAC_w

Cuts at:    752    3746     752 
   Size:       2994    1237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1077    1109    3748    3780    1077 
   Size:         32    2639      32    1528

  Fragments arranged by size: 

                2639    1528      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2026    2026 
   Size:       4231
 
XbaI T'CTAG_A

Cuts at:   1260    1260 
   Size:       4231
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1724    1724 
   Size:       4231
 
XmaI C'CCGG_G

Cuts at:   1249    1249 
   Size:       4231
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NruI
     NspI     PciI    PflMI     PfoI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI
     BmtI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsrGI   BssHII
   BstEII    BstXI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     MfeI     NcoI     NdeI     NheI     NotI     NsiI
     PacI     PasI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI     StuI
     SwaI     XhoI     XmnI     ZraI
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