Restriction map for pF1KSDA0976
 (Linear) (Six-base) MAPSORT of: KIAA0976.seq  Check: 7354  from: 1  to: 1092



KIAA0976.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0     640    1092 
   Size:        640     452
 
AflIII A'CryG_T

Cuts at:      0     257    1092 
   Size:        257     835
 
BamHI G'GATC_C

Cuts at:      0     564    1092 
   Size:        564     528
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339    1092 
   Size:        339     753
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     869     903    1092 
   Size:        869      34     189

  Fragments arranged by size: 

                 869     189      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     386     735    1072    1092 
   Size:        386     349     337      20

  Fragments arranged by size: 

                 386     349     337      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1061    1092 
   Size:       1061      31
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     505    1092 
   Size:        373     132     587

  Fragments arranged by size: 

                 587     373     132
 
Bpu10I CC'TnA_GC

Cuts at:      0     290     308    1092 
   Size:        290      18     784

  Fragments arranged by size: 

                 784     290      18
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212    1092 
   Size:        212     880
 
BsaHI Gr'CG_yC

Cuts at:      0     465    1092 
   Size:        465     627
 
BsaWI w'CCGG_w

Cuts at:      0     201    1092 
   Size:        201     891
 
BsiHKAI G_wGCw'C

Cuts at:      0     956    1092 
   Size:        956     136
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     223    1092 
   Size:        223     869
 
Bsp1286I G_dGCh'C

Cuts at:      0     956    1092 
   Size:        956     136
 
BspEI T'CCGG_A

Cuts at:      0     201    1092 
   Size:        201     891
 
BsrDI GCAATG_nn'

Cuts at:      0     248    1092 
   Size:        248     844
 
BstYI r'GATC_y

Cuts at:      0     564    1092 
   Size:        564     528
 
BstZ17I GTA'TAC

Cuts at:      0     641    1092 
   Size:        641     451
 
Bsu36I CC'TnA_GG

Cuts at:      0     784    1092 
   Size:        784     308
 
BtgI C'CryG_G

Cuts at:      0     161    1092 
   Size:        161     931
 
BtsI GCAGTG_nn'

Cuts at:      0    1046    1092 
   Size:       1046      46
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1013    1092 
   Size:       1013      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     617    1092 
   Size:        617     475
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     505    1092 
   Size:        373     132     587

  Fragments arranged by size: 

                 587     373     132
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     162     194     556     588    1092 
   Size:        162      32     362      32     504

  Fragments arranged by size: 

                 504     362     162      32      32
 
HincII GTy'rAC

Cuts at:      0     396    1092 
   Size:        396     696
 
HpaI GTT'AAC

Cuts at:      0     396    1092 
   Size:        396     696
 
MslI CAynn'nnrTG

Cuts at:      0     166     874     945    1092 
   Size:        166     708      71     147

  Fragments arranged by size: 

                 708     166     147      71
 
MspA1I CmG'CkG

Cuts at:      0     738    1092 
   Size:        738     354
 
NspI r_CATG'y

Cuts at:      0     261     944    1092 
   Size:        261     683     148

  Fragments arranged by size: 

                 683     261     148
 
PciI A'CATG_T

Cuts at:      0     257    1092 
   Size:        257     835
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     350     811     976    1092 
   Size:        350     461     165     116

  Fragments arranged by size: 

                 461     350     165     116
 
PfoI T'CCnGG_A

Cuts at:      0     482    1092 
   Size:        482     610
 
PvuII CAG'CTG

Cuts at:      0     738    1092 
   Size:        738     354
 
ScaI AGT'ACT

Cuts at:      0     628    1092 
   Size:        628     464
 
SfcI C'TryA_G

Cuts at:      0     962    1092 
   Size:        962     130
 
SspI AAT'ATT

Cuts at:      0     821    1092 
   Size:        821     271
 
StyI C'CwwG_G

Cuts at:      0    1009    1092 
   Size:       1009      83
 
TatI w'GTAC_w

Cuts at:      0     626    1092 
   Size:        626     466
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     951     983    1092 
   Size:        951      32     109

  Fragments arranged by size: 

                 951     109      32
 

 Enzymes that do cut:

     AccI   AflIII    BamHI     BbsI     BcgI    BciVI     BmrI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BsrDI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     DrdI     EarI
  Eco57MI    Hin4I   HincII     HpaI     MslI   MspA1I     NspI     PciI
    PflMI     PfoI    PvuII     ScaI     SfcI     SspI     StyI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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