Restriction map for pF1KSDA0969
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BalI        BbrPI       BclI        BfrI        
BlnI        BsaAI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        981  GGTACC               G^GTACC
AccI         2611  GTAGAC               GT^[AC][GT]AC
AccII        1798  CGCG                 CG^CG
AcyI         3115  GGCGCC               G[GA]^CG[CT]C
AflIII        371  ACACGT               A^C[GA][CT]GT
AlwNI        1708  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2821  CCTGAGG              CC^T[ACGT]AGG
Asp700I       994  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       981  GGTACC               G^GTACC
AspI         2764  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3115  GGCGCC               GGCGC^C
BbsI          994  GAAGAC               GAAGAC(2/6)
BcgI          119  GCAAAGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         296  GTATCC               GTATCC(6/5)
BglII        1952  AGATCT               A^GATCT
BlpI          382  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      382  GCTGAGC              GC^T[ACGT]AGC
BpuAI         994  GAAGAC               GAAGAC(2/6)
BsaHI        3115  GGCGCC               G[GA]^CG[CT]C
BsaI         1869  GGTCTC               GGTCTC(1/5)
BsmBI         857  CGTCTC               CGTCTC(1/5)
BssSI        2605  CACGAG               CACGAG(-5/-1)
BstEII       2346  GGTGACC              G^GT[ACGT]ACC
BstPI        2346  GGTGACC              G^GT[ACGT]ACC
BstUI        1798  CGCG                 CG^CG
BstXI         801  CCAGCCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2821  CCTGAGG              CC^T[ACGT]AGG
CelII         382  GCTGAGC              GC^T[ACGT]AGC
EagI         1108  CGGCCG               C^GGCCG
EclXI        1108  CGGCCG               C^GGCCG
Eco52I       1108  CGGCCG               C^GGCCG
Eco81I       2821  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3005  CCTCCCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2346  GGTGACC              G^GT[ACGT]ACC
HgaI           95  GCGTC                GACGC(5/10)
Hin1I        3115  GGCGCC               G[GA]^CG[CT]C
KasI         3115  GGCGCC               G^GCGCC
KpnI          981  GGTACC               GGTAC^C
MvnI         1798  CGCG                 CG^CG
NarI         3115  GGCGCC               GG^CGCC
NcoI          455  CCATGG               C^CATGG
PflFI        2764  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2554  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          195  GCTCTTC              GCTCTTC(1/4)
SfoI         3115  GGCGCC               GGC^GCC
SgrAI         883  CACCGGCG             C[GA]^CCGG[CT]G
Tth111I      2764  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           53  CCAACCACAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          994  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Aor51HI       140  AGCGCT               AGC^GCT
              236  AGCGCT              
ApaI          765  GGGCCC               GGGCC^C
             1160  GGGCCC              
BsaBI        1078  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2973  GATTGAAATC          
BsaWI         696  TCCGGT               [AT]^CCGG[AT]
              726  ACCGGA              
BsiEI        1108  CGGCCG               CG[GA][CT]^CG
             2996  CGACCG              
BsmI         2373  GAATGC               GAATGC(1/-1)
             2480  GAATGC              
DraIII       1360  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1427  CACCCAGTG           
Eco47III      140  AGCGCT               AGC^GCT
              236  AGCGCT              
MamI         1078  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2973  GATTGAAATC          
NaeI          833  GCCGGC               GCC^GGC
             1131  GCCGGC              
NgoMIV        833  GCCGGC               G^CCGGC
             1131  GCCGGC              
SacI          936  GAGCTC               GAGCT^C
             2749  GAGCTC              
ScaI         1046  AGTACT               AGT^ACT
             1088  AGTACT              
SfiI          665  GGCCTGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2538  GGCCAGCCCGGCC       
SmaI         2003  CCCGGG               CCC^GGG
             2678  CCCGGG              
XmaCI        2003  CCCGGG               C^CCGGG
             2678  CCCGGG              
XmaI         2003  CCCGGG               C^CCGGG
             2678  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BamHI       BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PaeR7I      
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoI        
XhoII       
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