Restriction map for pF1KSDA0969
 (Circular) (Six-base) MAPSORT of: pF1KSDA0969.seq  Check: 7246  from: 1  to: 6281



pF1KSDA0969  6281 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:   2738    3269    3319    2738 
   Size:        531      50    5700

  Fragments arranged by size: 

                5700     531      50
 
Acc65I G'GTAC_C

Cuts at:   1107    3297    1107 
   Size:       2190    4091
 
AclI AA'CG_TT

Cuts at:   6121    6121 
   Size:       6281
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    522    1063    1139    4106    4538    5285    5553     522 
   Size:        541      76    2967     432     747     268    1250

  Fragments arranged by size: 

                2967    1250     747     541     432     268      76
 
AfeI AGC'GCT

Cuts at:    268     364     268 
   Size:         96    6185
 
AflIII A'CryG_T

Cuts at:    497    3633    4737    5714     497 
   Size:       3136    1104     977    1064

  Fragments arranged by size: 

                3136    1104    1064     977
 
AgeI A'CCGG_T

Cuts at:   4669    4669 
   Size:       6281
 
AleI CACnn'nnGTG

Cuts at:   2621    2621 
   Size:       6281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2110    2142    2110 
   Size:         32    6249
 
AlwNI CAG_nnn'CTG

Cuts at:   1839    3586    5153    1839 
   Size:       1747    1567    2967

  Fragments arranged by size: 

                2967    1747    1567
 
ApaI G_GGCC'C

Cuts at:    895    1290     895 
   Size:        395    5886
 
ApaLI G'TGCA_C

Cuts at:   5051    5051 
   Size:       6281
 
ApoI r'AATT_y

Cuts at:   3285    5634    5890    3285 
   Size:       2349     256    3676

  Fragments arranged by size: 

                3676    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6281
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6281
 
AvaI C'yCGr_G

Cuts at:    553     596     746     886    1984    2129    2804    3301     553 
   Size:         43     150     140    1098     145     675     497    3533

  Fragments arranged by size: 

                3533    1098     675     497     150     145     140      43
            
 
BamHI G'GATC_C

Cuts at:    572    1394    2087    3306     572 
   Size:        822     693    1219    3547

  Fragments arranged by size: 

                3547    1219     822     693
 
BanI G'GyrC_C

Cuts at:   1107    2615    3024    3241    3297    3711    3959    3994    1107 
   Size:       1508     409     217      56     414     248      35    3394

  Fragments arranged by size: 

                3394    1508     414     409     248     217      56      35
            
 
BanII G_rGCy'C

Cuts at:    553     895    1066    1290    1361    1516    2242    2263    2300 
   Size:        342     171     224      71     155     726      21      37

Cuts at:   2300    2513    2846    2879    2947    3295    4325     553 
   Size:        213     333      33      68     348    1030    2509

  Fragments arranged by size: 

                2509    1030     726     348     342     333     224     213
                 171     155      71      68      37      33      21
 
BbeI G_GCGC'C

Cuts at:   3245    3715    3963    3245 
   Size:        470     248    5563

  Fragments arranged by size: 

                5563     470     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1127    1127 
   Size:       6281
 
BbvCI CC'TCA_GC

Cuts at:   2110    2172    2209    2110 
   Size:         62      37    6182

  Fragments arranged by size: 

                6182      62      37
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    234     268     234 
   Size:         34    6247
 
BciVI GTATCCnnnnn_n'

Cuts at:    433    4179    4940    6281     433 
   Size:       3746     761    1341     433

  Fragments arranged by size: 

                3746    1341     761     433
 
BclI T'GATC_A

Cuts at:   3801    3801 
   Size:       6281
 
BglI GCCn_nnn'nGGC

Cuts at:    798    2385    2671    2767    2784     798 
   Size:       1587     286      96      17    4295

  Fragments arranged by size: 

                4295    1587     286      96      17
 
BglII A'GATC_T

Cuts at:   2078    3796    5833    2078 
   Size:       1718    2037    2526

  Fragments arranged by size: 

                2526    2037    1718
 
BlpI GC'TnA_GC

Cuts at:    509    3393     509 
   Size:       2884    3397
 
Bme1580I G_kGCm'C

Cuts at:    632     895    1290    1496    1530    1563    2137    2381    2441 
   Size:        263     395     206      34      33     574     244      60

Cuts at:   2441    3029    3059    3906    3999    5055     632 
   Size:        588      30     847      93    1056    1858

  Fragments arranged by size: 

                1858    1056     847     588     574     395     263     244
                 206      93      60      34      33      30
 
BmgBI CAC'GTC

Cuts at:   2097    2859    3054    2097 
   Size:        762     195    5324

  Fragments arranged by size: 

                5324     762     195
 
BmrI ACTGGGnnnn_n'

Cuts at:   1010    1188    1550    2678    3493    3574    3909    6053    1010 
   Size:        178     362    1128     815      81     335    2144    1238

  Fragments arranged by size: 

                2144    1238    1128     815     362     335     178      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    879    3060    4648     879 
   Size:       2181    1588    2512

  Fragments arranged by size: 

                2512    2181    1588
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    191     596    1312    1861    2103    2773    2870    2944    3191 
   Size:        405     716     549     242     670      97      74     247

Cuts at:   3191     191 
   Size:       3281

  Fragments arranged by size: 

                3281     716     670     549     405     247     242      97
                  74
 
Bpu10I CC'TnA_GC

Cuts at:    445     722     728    2110    2172    2209    2236    2941     445 
   Size:        277       6    1382      62      37      27     705    3785

  Fragments arranged by size: 

                3785    1382     705     277      62      37      27       6
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3457    4336    4828    5126    3457 
   Size:        879     492     298    4612

  Fragments arranged by size: 

                4612     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2001    5985    2001 
   Size:       3984    2297
 
BsaAI yAC'GTr

Cuts at:   3273    3634    3273 
   Size:        361    5920
 
BsaBI GATnn'nnATC

Cuts at:      6    1208    3103    3795    5832       6 
   Size:       1202    1895     692    2037     455

  Fragments arranged by size: 

                2037    1895    1202     692     455
 
BsaHI Gr'CG_yC

Cuts at:   3242    3712    3960    4662    6158    3242 
   Size:        470     248     702    1496    3365

  Fragments arranged by size: 

                3365    1496     702     470     248
 
BsaWI w'CCGG_w

Cuts at:    822     852    3470    3991    4669    4943    5090    5501     822 
   Size:         30    2618     521     678     274     147     411    1602

  Fragments arranged by size: 

                2618    1602     678     521     411     274     147      30
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2110    2140    2110 
   Size:         30    6251
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    571    1179    2171    2517    2605    2661    2788    2965    3194 
   Size:        608     992     346      88      56     127     177     229

Cuts at:   3194     571 
   Size:       3658

  Fragments arranged by size: 

                3658     992     608     346     229     177     127      88
                  56
 
BseYI C'CCAG_C

Cuts at:    217     682     843     926    1052    1504    1725    2846    3706 
   Size:        465     161      83     126     452     221    1121     860

Cuts at:   3706    4464    5041    5537     217 
   Size:        758     577     496     961

  Fragments arranged by size: 

                1121     961     860     758     577     496     465     452
                 221     161     126      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    487    1798    1990     487 
   Size:       1311     192    4778

  Fragments arranged by size: 

                4778    1311     192
 
BsiEI CG_ry'CG

Cuts at:    104    1237    3125    3823    3869    5077     104 
   Size:       1133    1888     698      46    1208    1308

  Fragments arranged by size: 

                1888    1308    1208    1133     698      46
 
BsiHKAI G_wGCw'C

Cuts at:    973    1066    1982    2879    3295    4073    4263    5055     973 
   Size:         93     916     897     416     778     190     792    2199

  Fragments arranged by size: 

                2199     916     897     792     778     416     190      93
            
 
BsmI GAATG_Cn'

Cuts at:   2505    2612    2505 
   Size:        107    6174
 
BsmBI CGTCTCn'nnnn_

Cuts at:    989     989 
   Size:       6281
 
Bsp1286I G_dGCh'C

Cuts at:    553     632     895     973    1066    1290    1361    1496    1516 
   Size:         79     263      78      93     224      71     135      20

Cuts at:   1516    1530    1563    1982    2137    2242    2263    2300    2381 
   Size:         14      33     419     155     105      21      37      81

Cuts at:   2381    2441    2513    2846    2879    2947    3029    3059    3295 
   Size:         60      72     333      33      68      82      30     236

Cuts at:   3295    3906    3999    4073    4263    4325    5055     553 
   Size:        611      93      74     190      62     730    1779

  Fragments arranged by size: 

                1779     730     611     419     333     263     236     224
                 190     155     135     105      93      93      82      81
                  79      78      74      72      71      68      62      60
                  37      33      33      30      21      20      14
 
BspEI T'CCGG_A

Cuts at:   5501    5501 
   Size:       6281
 
BspHI T'CATG_A

Cuts at:   5457    5457 
   Size:       6281
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   3331    3545    3847    4228    3331 
   Size:        214     302     381    5384

  Fragments arranged by size: 

                5384     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     213    1481    1499    1550    1572    4573    4627    6111 
   Size:        163    1268      18      51      22    3001      54    1484

Cuts at:   6111    6276      50 
   Size:        165      55

  Fragments arranged by size: 

                3001    1484    1268     165     163      55      54      51
                  22      18
 
BsrDI GCAATG_nn'

Cuts at:   1323    2341    2996    3166    4193    1323 
   Size:       1018     655     170    1027    3411

  Fragments arranged by size: 

                3411    1027    1018     655     170
 
BsrFI r'CCGG_y

Cuts at:    959    1010    1257    1769    2060    4279    4460    4669     959 
   Size:         51     247     512     291    2219     181     209    2571

  Fragments arranged by size: 

                2571    2219     512     291     247     209     181      51
            
 
BssSI C'ACGA_G

Cuts at:   2731    4552    4910    2731 
   Size:       1821     358    4102

  Fragments arranged by size: 

                4102    1821     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3658    3658 
   Size:       6281
 
BstBI TT'CG_AA

Cuts at:    110    4642     110 
   Size:       4532    1749
 
BstEII G'GTnAC_C

Cuts at:   2472    2472 
   Size:       6281
 
BstXI CCAn_nnnn'nTGG

Cuts at:    934     934 
   Size:       6281
 
BstYI r'GATC_y

Cuts at:      1     572     605    1394    2078    2087    3306    3773    3796 
   Size:        571      33     789     684       9    1219     467      23

Cuts at:   3796    4131    4377    5389    5475    5487    5833       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1219    1012     789     684     571     467     449     346
                 335     246      86      33      23      12       9
 
Bsu36I CC'TnA_GG

Cuts at:   2948    2948 
   Size:       6281
 
BtgI C'CryG_G

Cuts at:    581    1946    3235     581 
   Size:       1365    1289    3627

  Fragments arranged by size: 

                3627    1365    1289
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    237    4231    4412    4575     237 
   Size:       3994     181     163    1943

  Fragments arranged by size: 

                3994    1943     181     163
 
BtsI GCAGTG_nn'

Cuts at:    358     470    1090    1593    1897    2492    3524     358 
   Size:        112     620     503     304     595    1032    3115

  Fragments arranged by size: 

                3115    1032     620     595     503     304     112
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1305    1340    1305 
   Size:         35    6246
 
DraI TTT'AAA

Cuts at:   3280    3280 
   Size:       6281
 
DraIII CAC_nnn'GTG

Cuts at:   1491    1558    1491 
   Size:         67    6214
 
DrdI GACnn_nn'nnGTC

Cuts at:   3987    4845    3987 
   Size:        858    5423
 
EaeI y'GGCC_r

Cuts at:    762     961    1234    2198    2663    2692    3866    4040    4431 
   Size:        199     273     964     465      29    1174     174     391

Cuts at:   4431    4458    5803     762 
   Size:         27    1345    1240

  Fragments arranged by size: 

                1345    1240    1174     964     465     391     273     199
                 174      29      27
 
EagI C'GGCC_G

Cuts at:   1234    3866    1234 
   Size:       2632    3649
 
EarI CTCTTCn'nnn_

Cuts at:    328     397     410    4304    4514     328 
   Size:         69      13    3894     210    2095

  Fragments arranged by size: 

                3894    2095     210      69      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1029    4489    4799    4945    6091    1029 
   Size:       3460     310     146    1146    1219

  Fragments arranged by size: 

                3460    1219    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1064    2877    3293    1064 
   Size:       1813     416    4052

  Fragments arranged by size: 

                4052    1813     416
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    191     522     596    1063    1139    1312    1861    2103    2773 
   Size:        331      74     467      76     173     549     242     670

Cuts at:   2773    2870    2944    3191    4106    4538    5285    5553     191 
   Size:         97      74     247     915     432     747     268     919

  Fragments arranged by size: 

                 919     915     747     670     549     467     432     331
                 268     247     242     173      97      76      74      74
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3135    3135 
   Size:       6281
 
EcoO109I rG'GnC_Cy

Cuts at:    201     891    1164    1287    1650    1826    2270    3420     201 
   Size:        690     273     123     363     176     444    1150    3062

  Fragments arranged by size: 

                3062    1150     690     444     363     273     176     123
            
 
EcoRI G'AATT_C

Cuts at:   3285    3285 
   Size:       6281
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4300    4300 
   Size:       6281
 
FspI TGC'GCA

Cuts at:   4062    4062 
   Size:       6281
 
HaeII r_GCGC'y

Cuts at:    270     366    1305    3245    3715    3963    4985    5964     270 
   Size:         96     939    1940     470     248    1022     979     587

  Fragments arranged by size: 

                1940    1022     979     939     587     470     248      96
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    590     622    1429    1461    4123    4153    4155    4185     590 
   Size:         32     807      32    2662      30       2      30    2686

  Fragments arranged by size: 

                2686    2662     807      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   3320    3320 
   Size:       6281
 
KasI G'GCGC_C

Cuts at:   3241    3711    3959    3241 
   Size:        470     248    5563

  Fragments arranged by size: 

                5563     470     248
 
KpnI G_GTAC'C

Cuts at:   1111    3301    1111 
   Size:       2190    4091
 
MluI A'CGCG_T

Cuts at:   5714    5714 
   Size:       6281
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2213    2315    2771    4952    5136    2213 
   Size:        102     456    2181     184    3358

  Fragments arranged by size: 

                3358    2181     456     184     102
 
MscI TGG'CCA

Cuts at:   4042    4042 
   Size:       6281
 
MslI CAynn'nnrTG

Cuts at:    650    1924    2621    4270    4397     650 
   Size:       1274     697    1649     127    2534

  Fragments arranged by size: 

                2534    1649    1274     697     127
 
MspA1I CmG'CkG

Cuts at:    847    1341    3078    3392    3706    4066    5079    5324     847 
   Size:        494    1737     314     314     360    1013     245    1804

  Fragments arranged by size: 

                1804    1737    1013     494     360     314     314     245
            
 
NaeI GCC'GGC

Cuts at:    961    1259    4462     961 
   Size:        298    3203    2780

  Fragments arranged by size: 

                3203    2780     298
 
NarI GG'CG_CC

Cuts at:   3242    3712    3960    3242 
   Size:        470     248    5563

  Fragments arranged by size: 

                5563     470     248
 
NcoI C'CATG_G

Cuts at:    581     581 
   Size:       6281
 
NgoMIV G'CCGG_C

Cuts at:    959    1257    4460     959 
   Size:        298    3203    2780

  Fragments arranged by size: 

                3203    2780     298
 
NspI r_CATG'y

Cuts at:    517    2791    3150    3334    3834    4741     517 
   Size:       2274     359     184     500     907    2057

  Fragments arranged by size: 

                2274    2057     907     500     359     184
 
PasI CC'CwG_GG

Cuts at:   1283    1283 
   Size:       6281
 
PciI A'CATG_T

Cuts at:   4737    4737 
   Size:       6281
 
PflMI CCAn_nnn'nTGG

Cuts at:    189     582     910     938    1391    1839     189 
   Size:        393     328      28     453     448    4631

  Fragments arranged by size: 

                4631     453     448     393     328      28
 
PfoI T'CCnGG_A

Cuts at:   1673    2090    1673 
   Size:        417    5864
 
PmeI GTTT'AAAC

Cuts at:   3280    3280 
   Size:       6281
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1233    1265    2110    2142    5420    5452    1233 
   Size:         32     845      32    3278      32    2062

  Fragments arranged by size: 

                3278    2062     845      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    201    1164    1650    1826    2270     201 
   Size:        963     486     176     444    4212

  Fragments arranged by size: 

                4212     963     486     444     176
 
PshAI GACnn'nnGTC

Cuts at:   2684    2684 
   Size:       6281
 
PspOMI G'GGCC_C

Cuts at:    891    1286     891 
   Size:        395    5886
 
PspXI vC'TCGA_Gb

Cuts at:    596     746    1984     596 
   Size:        150    1238    4893

  Fragments arranged by size: 

                4893    1238     150
 
PstI C_TGCA'G

Cuts at:   1475    1904    2657    3083    3328    4013    1475 
   Size:        429     753     426     245     685    3743

  Fragments arranged by size: 

                3743     753     685     429     426     245
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6281
 
PvuII CAG'CTG

Cuts at:    847    1341    3078    3706    4066     847 
   Size:        494    1737     628     360    3062

  Fragments arranged by size: 

                3062    1737     628     494     360
 
RsrII CG'GwC_CG

Cuts at:   4476    4476 
   Size:       6281
 
SacI G_AGCT'C

Cuts at:   1066    2879    3295    1066 
   Size:       1813     416    4052

  Fragments arranged by size: 

                4052    1813     416
 
SalI G'TCGA_C

Cuts at:   3318    3318 
   Size:       6281
 
SanDI GG'GwC_CC

Cuts at:   1164    1826    2270    1164 
   Size:        662     444    5175

  Fragments arranged by size: 

                5175     662     444
 
SapI GCTCTTCn'nnn_

Cuts at:    328    4304    4514     328 
   Size:       3976     210    2095

  Fragments arranged by size: 

                3976    2095     210
 
SbfI CC_TGCA'GG

Cuts at:   3328    3328 
   Size:       6281
 
ScaI AGT'ACT

Cuts at:   1174    1216    5798    1174 
   Size:         42    4582    1657

  Fragments arranged by size: 

                4582    1657      42
 
SfcI C'TryA_G

Cuts at:     33    1471    1518    1900    2181    2653    3079    3324    4009 
   Size:       1438      47     382     281     472     426     245     685

Cuts at:   4009    5002    5193      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1438    1121     993     685     472     426     382     281
                 245     191      47
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    798    2671     798 
   Size:       1873    4408
 
SfoI GGC'GCC

Cuts at:   3243    3713    3961    3243 
   Size:        470     248    5563

  Fragments arranged by size: 

                5563     470     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6281
 
SgrAI Cr'CCGG_yG

Cuts at:   1010    1010 
   Size:       6281
 
SmaI CCC'GGG

Cuts at:   2131    2806    3303    2131 
   Size:        675     497    5109

  Fragments arranged by size: 

                5109     675     497
 
SmlI C'TyrA_G

Cuts at:    596     746    1984    3436    4351    4843    5105     596 
   Size:        150    1238    1452     915     492     262    1772

  Fragments arranged by size: 

                1772    1452    1238     915     492     262     150
 
SnaBI TAC'GTA

Cuts at:   3273    3273 
   Size:       6281
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6281
 
SphI G_CATG'C

Cuts at:    517    2791    3150    3334    3834     517 
   Size:       2274     359     184     500    2964

  Fragments arranged by size: 

                2964    2274     500     359     184
 
StuI AGG'CCT

Cuts at:    568     792    3130     568 
   Size:        224    2338    3719

  Fragments arranged by size: 

                3719    2338     224
 
StyI C'CwwG_G

Cuts at:    308     581    1646    2300    3415    3769     308 
   Size:        273    1065     654    1115     354    2820

  Fragments arranged by size: 

                2820    1115    1065     654     354     273
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    696    1077    1518    1569    2056    2618    3139    3864    3997 
   Size:        381     441      51     487     562     521     725     133

Cuts at:   3997    4456    4665     696 
   Size:        459     209    2312

  Fragments arranged by size: 

                2312     725     562     521     487     459     441     381
                 209     133      51
 
TatI w'GTAC_w

Cuts at:    600    1172    1214    5796     600 
   Size:        572      42    4582    1085

  Fragments arranged by size: 

                4582    1085     572      42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2490    2522    5798    5830    2490 
   Size:         32    3276      32    2941

  Fragments arranged by size: 

                3276    2941      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2893    4078    2893 
   Size:       1185    5096
 
XbaI T'CTAG_A

Cuts at:   3312    3312 
   Size:       6281
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    186    3776     186 
   Size:       3590    2691
 
XhoI C'TCGA_G

Cuts at:    596     746    1984     596 
   Size:        150    1238    4893

  Fragments arranged by size: 

                4893    1238     150
 
XmaI C'CCGG_G

Cuts at:   2129    2804    3301    2129 
   Size:        675     497    5109

  Fragments arranged by size: 

                5109     675     497
 
XmnI GAAnn'nnTTC

Cuts at:   1124    1124 
   Size:       6281
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AlfI     AscI    AvrII     BaeI
    BfrBI     BmtI    BsiWI    BsrGI   BssHII  BstZ17I     ClaI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmlI     PsiI     PsrI    SacII    SexAI
     SrfI     SspI     SwaI     ZraI
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