Restriction map for
pF1KSDA0923
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BciVI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 632 GTCTAC GT^[AC][GT]AC
AflIII 1645 ACACGT A^C[GA][CT]GT
AlwNI 920 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1479 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 36 GTCTTC GAAGAC(2/6)
BcgI 137 CGAAAAATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 207 TGATCA T^GATCA
BcnI 953 CCGGG CC^[GC]GG
BpuAI 36 GTCTTC GAAGAC(2/6)
BsaI 1574 GGTCTC GGTCTC(1/5)
BseRI 1724 GAGGAG GAGGAG(10/8)
BsgI 168 CTGCAC GTGCAG(16/14)
Bsp1407I 1732 TGTACA T^GTACA
BspHI 585 TCATGA T^CATGA
BsrDI 558 CATTGC GCAATG(2/0)
BsrGI 1732 TGTACA T^GTACA
BstYI 24 AGATCC [GA]^GATC[CT]
Cac8I 1221 GCTCGC GC[ACGT]^[ACGT]GC
DraI 1315 TTTAAA TTT^AAA
DraII 980 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1558 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 440 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 980 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 207 TGATCA T^GATCA
HapII 953 CCGG C^CGG
HinfI 250 GAATC G^A[ACGT]TC
HpaII 953 CCGG C^CGG
HphI 1618 TCACC GGTGA(8/7)
MflI 24 AGATCC [GA]^GATC[CT]
MspA1I 328 CAGCGG C[AC]G^C[GT]G
MspI 953 CCGG C^CGG
NciI 953 CCGGG CC^[GC]GG
NdeI 1232 CATATG CA^TATG
NspI 1586 GCATGT [GA]CATG^[CT]
PpuMI 980 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 585 TCATGA T^CATGA
SspBI 1732 TGTACA T^GTACA
SspI 181 AATATT AAT^ATT
TfiI 250 GAATC G^A[AT]TC
XhoII 24 AGATCC [GA]^GATC[CT]
XmnI 1479 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 421 CTTAAG C^TTAAG
829 CTTAAG
Alw44I 145 GTGCAC G^TGCAC
1528 GTGCAC
AlwI 25 GATCC GGATC(4/5)
1768 GGATC
ApaLI 145 GTGCAC G^TGCAC
1528 GTGCAC
AspHI 145 GTGCAC G[AT]GC[AT]^C
1528 GTGCAC
AvaI 1156 CTCGAG C^[CT]CG[GA]G
1533 CTCGAG
BfaI 343 CTAG C^TAG
896 CTAG
BfrI 421 CTTAAG C^TTAAG
829 CTTAAG
BmyI 145 GTGCAC G[AGT]GC[ACT]^C
1528 GTGCAC
BsaJI 953 CCGGGG C^C[ACGT][ACGT]GG
1377 CCTGGG
BsiHKAI 145 GTGCAC G[AT]GC[AT]^C
1528 GTGCAC
BsiYI 440 CCTCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1377 CCTGGGATTGG
BslI 440 CCTCCAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1377 CCTGGGATTGG
BsmBI 1291 CGTCTC CGTCTC(1/5)
1347 GAGACG
BsoBI 1156 CTCGAG C^[CT]CG[GA]G
1533 CTCGAG
Bsp1286I 145 GTGCAC G[AGT]GC[ACT]^C
1528 GTGCAC
BstF5I 11 GGATG GGATG(2/0)
1792 GGATG
FokI 11 GGATG GGATG(9/13)
1792 GGATG
HindIII 1063 AAGCTT A^AGCTT
1353 AAGCTT
MaeI 343 CTAG C^TAG
896 CTAG
MaeIII 19 GTTAC ^GT[ACGT]AC
483 GTTAC
PaeR7I 1156 CTCGAG C^TCGAG
1533 CTCGAG
SfcI 400 CTACAG C^T[GA][CT]AG
1785 CTATAG
XhoI 1156 CTCGAG C^TCGAG
1533 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BpmI BsmAI BsmFI
BsrI BssKI BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I