Restriction map for
pF1KSDA0923
(Circular) (Six-base) MAPSORT of: pF1KSDA0923.seq Check: 6344 from: 1 to: 4981
pF1KSDA0923 4981 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 759 2017 759
Size: 1258 3723
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4981
AclI AA'CG_TT
Cuts at: 4821 4821
Size: 4981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 436 574 769 887 1739 1844 2804 3236
Size: 287 138 195 118 852 105 960 432
Cuts at: 3236 3985 4253 149
Size: 749 268 877
Fragments arranged by size:
960 877 852 749 432 287 268 195
138 118 105
AflII C'TTAA_G
Cuts at: 547 955 547
Size: 408 4573
AflIII A'CryG_T
Cuts at: 1771 2331 3437 4414 1771
Size: 560 1106 977 2338
Fragments arranged by size:
2338 1106 977 560
AgeI A'CCGG_T
Cuts at: 3369 3369
Size: 4981
AlwNI CAG_nnn'CTG
Cuts at: 1051 2284 3853 1051
Size: 1233 1569 2179
Fragments arranged by size:
2179 1569 1233
ApaLI G'TGCA_C
Cuts at: 271 1654 3751 271
Size: 1383 2097 1501
Fragments arranged by size:
2097 1501 1383
ApoI r'AATT_y
Cuts at: 495 627 896 1166 1292 1458 1938 1983 4334
Size: 132 269 270 126 166 480 45 2351
Cuts at: 4334 4590 495
Size: 256 886
Fragments arranged by size:
2351 886 480 270 269 256 166 132
126 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 4981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4981
AvaI C'yCGr_G
Cuts at: 1282 1659 1999 1282
Size: 377 340 4264
Fragments arranged by size:
4264 377 340
BamHI G'GATC_C
Cuts at: 2004 2004
Size: 4981
BanI G'GyrC_C
Cuts at: 1995 2409 2657 2692 1995
Size: 414 248 35 4284
Fragments arranged by size:
4284 414 248 35
BanII G_rGCy'C
Cuts at: 1993 3023 1993
Size: 1030 3951
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4733
BbsI GAAGACnn'nnnn_
Cuts at: 155 155
Size: 4981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 252 286 252
Size: 34 4947
BciVI GTATCCnnnnn_n'
Cuts at: 2877 3640 4981 2877
Size: 763 1341 2877
Fragments arranged by size:
2877 1341 763
BclI T'GATC_A
Cuts at: 333 2499 333
Size: 2166 2815
BglII A'GATC_T
Cuts at: 2494 4533 2494
Size: 2039 2942
BlpI GC'TnA_GC
Cuts at: 2091 2091
Size: 4981
Bme1580I G_kGCm'C
Cuts at: 275 1658 2604 2697 3755 275
Size: 1383 946 93 1058 1501
Fragments arranged by size:
1501 1383 1058 946 93
BmrI ACTGGGnnnn_n'
Cuts at: 2191 2272 2607 4753 2191
Size: 81 335 2146 2419
Fragments arranged by size:
2419 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 4981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 870 1688 552
Size: 318 818 3845
Fragments arranged by size:
3845 818 318
Bpu10I CC'TnA_GC
Cuts at: 473 473
Size: 4981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1947 2155 3034 3528 3826 1947
Size: 208 879 494 298 3102
Fragments arranged by size:
3102 879 494 298 208
BsaI GGTCTCn'nnnn_
Cuts at: 1706 4685 1706
Size: 2979 2002
BsaAI yAC'GTr
Cuts at: 1971 2332 1971
Size: 361 4620
BsaBI GATnn'nnATC
Cuts at: 6 2493 4532 6
Size: 2487 2039 455
Fragments arranged by size:
2487 2039 455
BsaHI Gr'CG_yC
Cuts at: 2410 2658 3362 4858 2410
Size: 248 704 1496 2533
Fragments arranged by size:
2533 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2168 2689 3369 3643 3790 4201 2168
Size: 521 680 274 147 411 2948
Fragments arranged by size:
2948 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 843 873 843
Size: 30 4951
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1865 1865
Size: 4981
BseYI C'CCAG_C
Cuts at: 504 2404 3162 3741 4237 504
Size: 1900 758 579 496 1248
Fragments arranged by size:
1900 1248 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 279
Size: 4981
BsiEI CG_ry'CG
Cuts at: 104 2521 2567 3777 104
Size: 2417 46 1210 1308
Fragments arranged by size:
2417 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 275 1658 1993 2771 2961 3755 275
Size: 1383 335 778 190 794 1501
Fragments arranged by size:
1501 1383 794 778 335 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1423 1467 1423
Size: 44 4937
Bsp1286I G_dGCh'C
Cuts at: 275 1658 1993 2604 2697 2771 2961 3023 3755
Size: 1383 335 611 93 74 190 62 732
Cuts at: 3755 275
Size: 1501
Fragments arranged by size:
1501 1383 732 611 335 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4201 4201
Size: 4981
BspHI T'CATG_A
Cuts at: 711 4157 711
Size: 3446 1535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2029 2243 2545 2926 2029
Size: 214 302 381 4084
Fragments arranged by size:
4084 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3271 3325 4811 4976 50
Size: 3221 54 1486 165 55
Fragments arranged by size:
3221 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 683 2891 683
Size: 2208 2773
BsrFI r'CCGG_y
Cuts at: 2977 3158 3369 2977
Size: 181 211 4589
Fragments arranged by size:
4589 211 181
BsrGI T'GTAC_A
Cuts at: 1858 1858
Size: 4981
BssSI C'ACGA_G
Cuts at: 3250 3610 3250
Size: 360 4621
BstAPI GCAn_nnn'nTGC
Cuts at: 2356 2356
Size: 4981
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4981
BstYI r'GATC_y
Cuts at: 1 150 2004 2471 2494 2829 3075 4089 4175
Size: 149 1854 467 23 335 246 1014 86
Cuts at: 4175 4187 4533 1
Size: 12 346 449
Fragments arranged by size:
1854 1014 467 449 346 335 246 149
86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4981
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4637
Fragments arranged by size:
4637 181 163
BtsI GCAGTG_nn'
Cuts at: 1306 2222 1306
Size: 916 4065
DraI TTT'AAA
Cuts at: 1443 1978 1443
Size: 535 4446
DraIII CAC_nnn'GTG
Cuts at: 1689 1689
Size: 4981
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3545 2685
Size: 860 4121
EaeI y'GGCC_r
Cuts at: 2564 2738 3129 3156 4503 2564
Size: 174 391 27 1347 3042
Fragments arranged by size:
3042 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4981
EarI CTCTTCn'nnn_
Cuts at: 715 1001 1739 3002 3212 715
Size: 286 738 1263 210 2484
Fragments arranged by size:
2484 1263 738 286 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3187 3499 3645 4791 3187
Size: 312 146 1146 3377
Fragments arranged by size:
3377 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4981
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 436 552 574 769 870 887 1688 1739
Size: 287 116 22 195 101 17 801 51
Cuts at: 1739 1844 2804 3236 3985 4253 149
Size: 105 960 432 749 268 877
Fragments arranged by size:
960 877 801 749 432 287 268 195
116 105 101 51 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 570 570
Size: 4981
EcoO109I rG'GnC_Cy
Cuts at: 1107 2118 1107
Size: 1011 3970
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4981
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4981
HaeII r_GCGC'y
Cuts at: 2413 2661 3685 4664 2413
Size: 248 1024 979 2730
Fragments arranged by size:
2730 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1263 1305 1337 2821 2851 2853 2883 1231
Size: 32 42 32 1484 30 2 30 3329
Fragments arranged by size:
3329 1484 42 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2018 2018
Size: 4981
HindIII A'AGCT_T
Cuts at: 1189 1479 1189
Size: 290 4691
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4733
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4981
MluI A'CGCG_T
Cuts at: 4414 4414
Size: 4981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1382 1827 3652 3836 1382
Size: 445 1825 184 2527
Fragments arranged by size:
2527 1825 445 184
MscI TGG'CCA
Cuts at: 2740 2740
Size: 4981
MslI CAynn'nnrTG
Cuts at: 2968 3095 2968
Size: 127 4854
MspA1I CmG'CkG
Cuts at: 456 2090 2404 2764 3779 4024 456
Size: 1634 314 360 1015 245 1413
Fragments arranged by size:
1634 1413 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4981
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4733
NcoI C'CATG_G
Cuts at: 125 125
Size: 4981
NdeI CA'TA_TG
Cuts at: 1359 1359
Size: 4981
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4981
NruI TCG'CGA
Cuts at: 3342 3342
Size: 4981
NspI r_CATG'y
Cuts at: 1716 2032 2532 3441 1716
Size: 316 500 909 3256
Fragments arranged by size:
3256 909 500 316
PciI A'CATG_T
Cuts at: 3437 3437
Size: 4981
PfoI T'CCnGG_A
Cuts at: 568 847 568
Size: 279 4702
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4120 4152 4120
Size: 32 4949
PpuMI rG'GwC_Cy
Cuts at: 1107 1107
Size: 4981
PspXI vC'TCGA_Gb
Cuts at: 1282 1659 1282
Size: 377 4604
PstI C_TGCA'G
Cuts at: 2026 2711 2026
Size: 685 4296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4981
PvuII CAG'CTG
Cuts at: 2404 2764 2404
Size: 360 4621
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4981
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4981
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4981
SapI GCTCTTCn'nnn_
Cuts at: 3002 3212 3002
Size: 210 4771
SbfI CC_TGCA'GG
Cuts at: 2026 2026
Size: 4981
ScaI AGT'ACT
Cuts at: 4498 4498
Size: 4981
SfcI C'TryA_G
Cuts at: 33 526 1911 2022 2707 3702 3893 33
Size: 493 1385 111 685 995 191 1121
Fragments arranged by size:
1385 1121 995 685 493 191 111
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4733
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4981
SmaI CCC'GGG
Cuts at: 2001 2001
Size: 4981
SmlI C'TyrA_G
Cuts at: 547 955 1282 1659 1926 2134 3049 3543 3805
Size: 408 327 377 267 208 915 494 262
Cuts at: 3805 547
Size: 1723
Fragments arranged by size:
1723 915 494 408 377 327 267 262
208
SnaBI TAC'GTA
Cuts at: 1971 1971
Size: 4981
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4981
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4481
SspI AAT'ATT
Cuts at: 309 309
Size: 4981
StyI C'CwwG_G
Cuts at: 125 2113 2467 125
Size: 1988 354 2639
Fragments arranged by size:
2639 1988 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2562 2695 3154 3365 2562
Size: 133 459 211 4178
Fragments arranged by size:
4178 459 211 133
TatI w'GTAC_w
Cuts at: 420 1042 1806 1858 4496 420
Size: 622 764 52 2638 905
Fragments arranged by size:
2638 905 764 622 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4498 4530 4498
Size: 32 4949
Tth111I GACn'n_nGTC
Cuts at: 2776 2776
Size: 4981
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2474 2474
Size: 4981
XhoI C'TCGA_G
Cuts at: 1282 1659 1282
Size: 377 4604
XmaI C'CCGG_G
Cuts at: 1999 1999
Size: 4981
XmnI GAAnn'nnTTC
Cuts at: 1609 1609
Size: 4981
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI ZraI