Restriction map for pF1KSDA0881
 (Circular) (Six-base) MAPSORT of: pF1KSDA0881.seq  Check: 1231  from: 1  to: 6284



pF1KSDA0881  6284 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    666     666 
   Size:       6284
 
AatII G_ACGT'C

Cuts at:    749     749 
   Size:       6284
 
AccI GT'mk_AC

Cuts at:    251     899    3322     251 
   Size:        648    2423    3213

  Fragments arranged by size: 

                3213    2423     648
 
Acc65I G'GTAC_C

Cuts at:   3300    3300 
   Size:       6284
 
AclI AA'CG_TT

Cuts at:   6124    6124 
   Size:       6284
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     478     517     895     973    1328    1927    2921    4109 
   Size:        303      39     378      78     355     599     994    1188

Cuts at:   4109    4541    5288    5556     175 
   Size:        432     747     268     903

  Fragments arranged by size: 

                1188     994     903     747     599     432     378     355
                 303     268      78      39
 
AflII C'TTAA_G

Cuts at:   2479    2479 
   Size:       6284
 
AflIII A'CryG_T

Cuts at:   2395    3636    4740    5717    2395 
   Size:       1241    1104     977    2962

  Fragments arranged by size: 

                2962    1241    1104     977
 
AgeI A'CCGG_T

Cuts at:   4672    4672 
   Size:       6284
 
AhdI GACnn_n'nnGTC

Cuts at:    750     750 
   Size:       6284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1598    2092    2336    1598 
   Size:        494     244    5546

  Fragments arranged by size: 

                5546     494     244
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    343     375    1798    1830    2584    2616     343 
   Size:         32    1423      32     754      32    4011

  Fragments arranged by size: 

                4011    1423     754      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    463     540    1644    2106    2571    2634    3141    3589    5156 
   Size:         77    1104     462     465      63     507     448    1567

Cuts at:   5156     463 
   Size:       1591

  Fragments arranged by size: 

                1591    1567    1104     507     465     462     448      77
                  63
 
ApaI G_GGCC'C

Cuts at:   3077    3187    3212    3077 
   Size:        110      25    6149

  Fragments arranged by size: 

                6149     110      25
 
ApaLI G'TGCA_C

Cuts at:    478    1688    2156    5054     478 
   Size:       1210     468    2898    1708

  Fragments arranged by size: 

                2898    1708    1210     468
 
ApoI r'AATT_y

Cuts at:    891    3288    5637    5893     891 
   Size:       2397    2349     256    1282

  Fragments arranged by size: 

                2397    2349    1282     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6284
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6284
 
AvaI C'yCGr_G

Cuts at:    260     697    1168    1313    3156    3205    3304     260 
   Size:        437     471     145    1843      49      99    3240

  Fragments arranged by size: 

                3240    1843     471     437     145      99      49
 
BamHI G'GATC_C

Cuts at:   3309    3309 
   Size:       6284
 
BanI G'GyrC_C

Cuts at:    654     676     693    1083    1099    1760    3201    3300    3714 
   Size:         22      17     390      16     661    1441      99     414

Cuts at:   3714    3962    3997     654 
   Size:        248      35    2941

  Fragments arranged by size: 

                2941    1441     661     414     390     248      99      35
                  22      17      16
 
BanII G_rGCy'C

Cuts at:    179     457    1136    1390    1919    2240    3077    3111    3187 
   Size:        278     679     254     529     321     837      34      76

Cuts at:   3187    3212    3298    4328     179 
   Size:         25      86    1030    2135

  Fragments arranged by size: 

                2135    1030     837     679     529     321     278     254
                  86      76      34      25
 
BbeI G_GCGC'C

Cuts at:   3718    3966    3718 
   Size:        248    6036
 
BbsI GAAGACnn'nnnn_

Cuts at:    901     901 
   Size:       6284
 
BbvCI CC'TCA_GC

Cuts at:   1663    1663 
   Size:       6284
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3213    3247    3213 
   Size:         34    6250
 
BciVI GTATCCnnnnn_n'

Cuts at:   4182    4943    6284    4182 
   Size:        761    1341    4182

  Fragments arranged by size: 

                4182    1341     761
 
BclI T'GATC_A

Cuts at:   3804    3804 
   Size:       6284
 
BglI GCCn_nnn'nGGC

Cuts at:   3144    3144 
   Size:       6284
 
BglII A'GATC_T

Cuts at:   3799    5836    3799 
   Size:       2037    4247
 
BlpI GC'TnA_GC

Cuts at:    173     456    1600    2234    2462    2557    3396     173 
   Size:        283    1144     634     228      95     839    3061

  Fragments arranged by size: 

                3061    1144     839     634     283     228      95
 
Bme1580I G_kGCm'C

Cuts at:    482     679    1190    1692    2160    2739    2979    3077    3187 
   Size:        197     511     502     468     579     240      98     110

Cuts at:   3187    3206    3212    3909    4002    5058     482 
   Size:         19       6     697      93    1056    1708

  Fragments arranged by size: 

                1708    1056     697     579     511     502     468     240
                 197     110      98      93      19       6
 
BmgBI CAC'GTC

Cuts at:   2396    2396 
   Size:       6284
 
BmrI ACTGGGnnnn_n'

Cuts at:   3496    3577    3912    6056    3496 
   Size:         81     335    2144    3724

  Fragments arranged by size: 

                3724    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1596    4651    1596 
   Size:       3055    3229
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    844    1194    1426    1672    1885    1903    1969    2194    2215 
   Size:        350     232     246     213      18      66     225      21

Cuts at:   2215    2299    2464    2656    2731    2764    2856    3002    3003 
   Size:         84     165     192      75      33      92     146       1

Cuts at:   3003     844 
   Size:       4125

  Fragments arranged by size: 

                4125     350     246     232     225     213     192     165
                 146      92      84      75      66      33      21      18
                   1
 
Bpu10I CC'TnA_GC

Cuts at:   1663    3214    1663 
   Size:       1551    4733
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    908    1729    2473    2833    3460    4339    4831    5129     908 
   Size:        821     744     360     627     879     492     298    2063

  Fragments arranged by size: 

                2063     879     821     744     627     492     360     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1508    2440    2578    5988    1508 
   Size:        932     138    3410    1804

  Fragments arranged by size: 

                3410    1804     932     138
 
BsaAI yAC'GTr

Cuts at:   3276    3637    3276 
   Size:        361    5923
 
BsaBI GATnn'nnATC

Cuts at:      6    2668    3798    5835       6 
   Size:       2662    1130    2037     455

  Fragments arranged by size: 

                2662    2037    1130     455
 
BsaHI Gr'CG_yC

Cuts at:    746    3027    3715    3963    4665    6161     746 
   Size:       2281     688     248     702    1496     869

  Fragments arranged by size: 

                2281    1496     869     702     688     248
 
BsaWI w'CCGG_w

Cuts at:    269     887    3473    3994    4672    4946    5093    5504     269 
   Size:        618    2586     521     678     274     147     411    1049

  Fragments arranged by size: 

                2586    1049     678     618     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    343     356     373     386    1798    1828    2584    2614     343 
   Size:         13      17      13    1412      30     756      30    4013

  Fragments arranged by size: 

                4013    1412     756      30      30      17      13      13
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1129    1138    1279    1282    1285    1288    1291    1300    1303 
   Size:          9     141       3       3       3       3       9       3

Cuts at:   1303    1306    1693    1819    1927    2017    2776    2987    1129 
   Size:          3     387     126     108      90     759     211    4426

  Fragments arranged by size: 

                4426     759     387     211     141     126     108      90
                   9       9       3       3       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:    133    1189    1432    1458    1930    2265    2862    2940    3069 
   Size:       1056     243      26     472     335     597      78     129

Cuts at:   3069    3134    3709    4467    5044    5540     133 
   Size:         65     575     758     577     496     877

  Fragments arranged by size: 

                1056     877     758     597     577     575     496     472
                 335     243     129      78      65      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    535    2239     535 
   Size:       1704    4580
 
BsiEI CG_ry'CG

Cuts at:    104    3826    3872    5080     104 
   Size:       3722      46    1208    1308

  Fragments arranged by size: 

                3722    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    179     422     482     860     971    1346    1685    1692    1919 
   Size:        243      60     378     111     375     339       7     227

Cuts at:   1919    2160    2240    2436    3298    4076    4266    5058     179 
   Size:        241      80     196     862     778     190     792    1405

  Fragments arranged by size: 

                1405     862     792     778     378     375     339     243
                 241     227     196     190     111      80      60       7
            
 
BsmI GAATG_Cn'

Cuts at:    816     816 
   Size:       6284
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2386    3041    2386 
   Size:        655    5629
 
Bsp1286I G_dGCh'C

Cuts at:    179     422     457     482     679     860     971    1136    1190 
   Size:        243      35      25     197     181     111     165      54

Cuts at:   1190    1346    1390    1685    1692    1919    2160    2240    2436 
   Size:        156      44     295       7     227     241      80     196

Cuts at:   2436    2739    2979    3077    3111    3187    3206    3212    3298 
   Size:        303     240      98      34      76      19       6      86

Cuts at:   3298    3909    4002    4076    4266    4328    5058     179 
   Size:        611      93      74     190      62     730    1405

  Fragments arranged by size: 

                1405     730     611     303     295     243     241     240
                 227     197     196     190     181     165     156     111
                  98      93      86      80      76      74      62      54
                  44      35      34      25      19       7       6
 
BspEI T'CCGG_A

Cuts at:    269     887    5504     269 
   Size:        618    4617    1049

  Fragments arranged by size: 

                4617    1049     618
 
BspHI T'CATG_A

Cuts at:   5460    5460 
   Size:       6284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    666     775    1052    2114    3334    3548    3850    4231     666 
   Size:        109     277    1062    1220     214     302     381    2719

  Fragments arranged by size: 

                2719    1220    1062     381     302     277     214     109
            
 
BsrBI CCG'CTC

Cuts at:     50     798     978    1604    1809    2817    4576    4630    6114 
   Size:        748     180     626     205    1008    1759      54    1484

Cuts at:   6114    6279      50 
   Size:        165      55

  Fragments arranged by size: 

                1759    1484    1008     748     626     205     180     165
                  55      54
 
BsrDI GCAATG_nn'

Cuts at:    833    4196     833 
   Size:       3363    2921
 
BsrFI r'CCGG_y

Cuts at:   1376    4282    4463    4672    1376 
   Size:       2906     181     209    2988

  Fragments arranged by size: 

                2988    2906     209     181
 
BssSI C'ACGA_G

Cuts at:   1787    2176    2689    4555    4913    1787 
   Size:        389     513    1866     358    3158

  Fragments arranged by size: 

                3158    1866     513     389     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1792    2307    3661    1792 
   Size:        515    1354    4415

  Fragments arranged by size: 

                4415    1354     515
 
BstBI TT'CG_AA

Cuts at:    110    4645     110 
   Size:       4535    1749
 
BstYI r'GATC_y

Cuts at:      1    1067    1559    2402    2669    3309    3776    3799    4134 
   Size:       1066     492     843     267     640     467      23     335

Cuts at:   4134    4380    5392    5478    5490    5836       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1066    1012     843     640     492     467     449     346
                 335     267     246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    252     252 
   Size:       6284
 
Bsu36I CC'TnA_GG

Cuts at:    412     412 
   Size:       6284
 
BtgI C'CryG_G

Cuts at:    231     522     713    2988    3099    3197     231 
   Size:        291     191    2275     111      98    3318

  Fragments arranged by size: 

                3318    2275     291     191     111      98
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    829    4234    4415    4578     829 
   Size:       3405     181     163    2535

  Fragments arranged by size: 

                3405    2535     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1996    2056    2158    2737    3171    3177    3527    1996 
   Size:         60     102     579     434       6     350    4753

  Fragments arranged by size: 

                4753     579     434     350     102      60       6
 
DraI TTT'AAA

Cuts at:   3283    3283 
   Size:       6284
 
DraIII CAC_nnn'GTG

Cuts at:   1185    3088    3100    1185 
   Size:       1903      12    4369

  Fragments arranged by size: 

                4369    1903      12
 
DrdI GACnn_nn'nnGTC

Cuts at:   3990    4848    3990 
   Size:        858    5426
 
EaeI y'GGCC_r

Cuts at:    228     287     710     979    1322    3869    4043    4434    4461 
   Size:         59     423     269     343    2547     174     391      27

Cuts at:   4461    5806     228 
   Size:       1345     706

  Fragments arranged by size: 

                2547    1345     706     423     391     343     269     174
                  59      27
 
EagI C'GGCC_G

Cuts at:   3869    3869 
   Size:       6284
 
EarI CTCTTCn'nnn_

Cuts at:    184     211    1115    1256    1277    1485    2489    2753    4307 
   Size:         27     904     141      21     208    1004     264    1554

Cuts at:   4307    4517     184 
   Size:        210    1951

  Fragments arranged by size: 

                1951    1554    1004     904     264     210     208     141
                  27      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2014    2539    3119    4492    4802    4948    6094    2014 
   Size:        525     580    1373     310     146    1146    2204

  Fragments arranged by size: 

                2204    1373    1146     580     525     310     146
 
EcoICRI GAG'CTC

Cuts at:    177    1917    2238    3296     177 
   Size:       1740     321    1058    3165

  Fragments arranged by size: 

                3165    1740    1058     321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     478     517     844     895     973    1194    1328    1426 
   Size:        303      39     327      51      78     221     134      98

Cuts at:   1426    1672    1885    1903    1927    1969    2194    2215    2299 
   Size:        246     213      18      24      42     225      21      84

Cuts at:   2299    2464    2656    2731    2764    2856    2921    3002    3003 
   Size:        165     192      75      33      92      65      81       1

Cuts at:   3003    4109    4541    5288    5556     175 
   Size:       1106     432     747     268     903

  Fragments arranged by size: 

                1106     903     747     432     327     303     268     246
                 225     221     213     192     165     134      98      92
                  84      81      78      75      65      51      42      39
                  33      24      21      18       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:    496    1864    2098    3002     496 
   Size:       1368     234     904    3778

  Fragments arranged by size: 

                3778    1368     904     234
 
EcoO109I rG'GnC_Cy

Cuts at:    159     537    1107    1303    2103    2118    2607    3055    3073 
   Size:        378     570     196     800      15     489     448      18

Cuts at:   3073    3074    3184    3209    3423     159 
   Size:          1     110      25     214    3020

  Fragments arranged by size: 

                3020     800     570     489     448     378     214     196
                 110      25      18      15       1
 
EcoRI G'AATT_C

Cuts at:   3288    3288 
   Size:       6284
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    978    4303     978 
   Size:       3325    2959
 
FspI TGC'GCA

Cuts at:   4065    4065 
   Size:       6284
 
HaeII r_GCGC'y

Cuts at:    532    1208    2034    2572    3718    3966    4988    5967     532 
   Size:        676     826     538    1146     248    1022     979     849

  Fragments arranged by size: 

                1146    1022     979     849     826     676     538     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    736     768    2394    2426    4126    4156    4158    4188     736 
   Size:         32    1626      32    1700      30       2      30    2832

  Fragments arranged by size: 

                2832    1700    1626      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    900    1230    3323     900 
   Size:        330    2093    3861

  Fragments arranged by size: 

                3861    2093     330
 
KasI G'GCGC_C

Cuts at:   3714    3962    3714 
   Size:        248    6036
 
KpnI G_GTAC'C

Cuts at:   3304    3304 
   Size:       6284
 
MluI A'CGCG_T

Cuts at:   5717    5717 
   Size:       6284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4955    5139    4955 
   Size:        184    6100
 
MscI TGG'CCA

Cuts at:    230     289     712    1324    4045     230 
   Size:         59     423     612    2721    2469

  Fragments arranged by size: 

                2721    2469     612     423      59
 
MslI CAynn'nnrTG

Cuts at:    575    4273    4400     575 
   Size:       3698     127    2459

  Fragments arranged by size: 

                3698    2459     127
 
MspA1I CmG'CkG

Cuts at:    133     514    1599    1641    1701    1719    1905    2277    2751 
   Size:        381    1085      42      60      18     186     372     474

Cuts at:   2751    3199    3395    3709    4069    5082    5327     133 
   Size:        448     196     314     360    1013     245    1090

  Fragments arranged by size: 

                1090    1085    1013     474     448     381     372     360
                 314     245     196     186      60      42      18
 
NaeI GCC'GGC

Cuts at:   4465    4465 
   Size:       6284
 
NarI GG'CG_CC

Cuts at:   3715    3963    3715 
   Size:        248    6036
 
NcoI C'CATG_G

Cuts at:    231     713    2988     231 
   Size:        482    2275    3527

  Fragments arranged by size: 

                3527    2275     482
 
NgoMIV G'CCGG_C

Cuts at:   4463    4463 
   Size:       6284
 
NspI r_CATG'y

Cuts at:   1143    1197    2323    3012    3337    3837    4744    1143 
   Size:         54    1126     689     325     500     907    2683

  Fragments arranged by size: 

                2683    1126     907     689     500     325      54
 
PasI CC'CwG_GG

Cuts at:   2913    2913 
   Size:       6284
 
PciI A'CATG_T

Cuts at:   4740    4740 
   Size:       6284
 
PfoI T'CCnGG_A

Cuts at:    218     971    1562    1919    2387     218 
   Size:        753     591     357     468    4115

  Fragments arranged by size: 

                4115     753     591     468     357
 
PmeI GTTT'AAAC

Cuts at:   3283    3283 
   Size:       6284
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    343     375    1798    1830    2584    2616    5423    5455     343 
   Size:         32    1423      32     754      32    2807      32    1172

  Fragments arranged by size: 

                2807    1423    1172     754      32      32      32      32
            
 
PpuMI rG'GwC_Cy

Cuts at:    159    2103    2118     159 
   Size:       1944      15    4325

  Fragments arranged by size: 

                4325    1944      15
 
PshAI GACnn'nnGTC

Cuts at:    873     905     873 
   Size:         32    6252
 
PspOMI G'GGCC_C

Cuts at:   3073    3183    3208    3073 
   Size:        110      25    6149

  Fragments arranged by size: 

                6149     110      25
 
PstI C_TGCA'G

Cuts at:   1049    1455    1700    2051    2252    2372    2780    3331    4016 
   Size:        406     245     351     201     120     408     551     685

Cuts at:   4016    1049 
   Size:       3317

  Fragments arranged by size: 

                3317     685     551     408     406     351     245     201
                 120
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6284
 
PvuII CAG'CTG

Cuts at:    133     514    1599    1641    1905    2277    3709    4069     133 
   Size:        381    1085      42     264     372    1432     360    2348

  Fragments arranged by size: 

                2348    1432    1085     381     372     360     264      42
            
 
RsrII CG'GwC_CG

Cuts at:   4479    4479 
   Size:       6284
 
SacI G_AGCT'C

Cuts at:    179    1919    2240    3298     179 
   Size:       1740     321    1058    3165

  Fragments arranged by size: 

                3165    1740    1058     321
 
SacII CC_GC'GG

Cuts at:   3200    3200 
   Size:       6284
 
SalI G'TCGA_C

Cuts at:    898    3321     898 
   Size:       2423    3861
 
SapI GCTCTTCn'nnn_

Cuts at:    184     211    2489    4307    4517     184 
   Size:         27    2278    1818     210    1951

  Fragments arranged by size: 

                2278    1951    1818     210      27
 
SbfI CC_TGCA'GG

Cuts at:   3331    3331 
   Size:       6284
 
ScaI AGT'ACT

Cuts at:    883    2038    5801     883 
   Size:       1155    3763    1366

  Fragments arranged by size: 

                3763    1366    1155
 
SfcI C'TryA_G

Cuts at:     33     306    1045    1451    1696    2047    2248    2368    2776 
   Size:        273     739     406     245     351     201     120     408

Cuts at:   2776    3327    4012    5005    5196      33 
   Size:        551     685     993     191    1121

  Fragments arranged by size: 

                1121     993     739     685     551     408     406     351
                 273     245     201     191     120
 
SfoI GGC'GCC

Cuts at:   3716    3964    3716 
   Size:        248    6036
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6284
 
SmaI CCC'GGG

Cuts at:    262    1315    3207    3306     262 
   Size:       1053    1892      99    3240

  Fragments arranged by size: 

                3240    1892    1053      99
 
SmlI C'TyrA_G

Cuts at:    923    1168    1708    2479    2488    2812    3439    4354    4846 
   Size:        245     540     771       9     324     627     915     492

Cuts at:   4846    5108     923 
   Size:        262    2099

  Fragments arranged by size: 

                2099     915     771     627     540     492     324     262
                 245       9
 
SnaBI TAC'GTA

Cuts at:   3276    3276 
   Size:       6284
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6284
 
SphI G_CATG'C

Cuts at:   2323    3012    3337    3837    2323 
   Size:        689     325     500    4770

  Fragments arranged by size: 

                4770     689     500     325
 
SrfI GCCC'GGGC

Cuts at:   3207    3207 
   Size:       6284
 
StuI AGG'CCT

Cuts at:   2839    2839 
   Size:       6284
 
StyI C'CwwG_G

Cuts at:    231     713     755    1016    1830    2988    3418    3772     231 
   Size:        482      42     261     814    1158     430     354    2743

  Fragments arranged by size: 

                2743    1158     814     482     430     354     261      42
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    999    1101    3867    4000    4459    4668     999 
   Size:        102    2766     133     459     209    2615

  Fragments arranged by size: 

                2766    2615     459     209     133     102
 
TatI w'GTAC_w

Cuts at:    881    2036    2060    2282    2417    5799     881 
   Size:       1155      24     222     135    3382    1366

  Fragments arranged by size: 

                3382    1366    1155     222     135      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2137    2169    3084    3116    3253    3285    5801    5833    2137 
   Size:         32     915      32     137      32    2516      32    2588

  Fragments arranged by size: 

                2588    2516     915     137      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   4081    4081 
   Size:       6284
 
XbaI T'CTAG_A

Cuts at:    471    3315     471 
   Size:       2844    3440
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    238     669     993    2190    2880    3247    3779     238 
   Size:        431     324    1197     690     367     532    2743

  Fragments arranged by size: 

                2743    1197     690     532     431     367     324
 
XhoI C'TCGA_G

Cuts at:   1168    1168 
   Size:       6284
 
XmaI C'CCGG_G

Cuts at:    260    1313    3205    3304     260 
   Size:       1053    1892      99    3240

  Fragments arranged by size: 

                3240    1892    1053      99
 
XmnI GAAnn'nnTTC

Cuts at:   2383    2383 
   Size:       6284
 
ZraI GAC'GTC

Cuts at:    747     747 
   Size:       6284
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AleI     AscI    AvrII     BaeI    BfrBI     BmtI    BsiWI
    BsrGI   BssHII   BstEII    BstXI     ClaI    CspCI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmlI     PsiI    PspXI     PsrI    SanDI
    SexAI     SfiI    SgrAI     SspI     SwaI
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