Restriction map for pF1KSDA0871
 (Circular) (Six-base) MAPSORT of: pF1KSDA0871.seq  Check: 6945  from: 1  to: 4546



pF1KSDA0871  4546 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AccI GT'mk_AC

Cuts at:   1582    1582 
   Size:       4546
 
Acc65I G'GTAC_C

Cuts at:   1560    1560 
   Size:       4546
 
AclI AA'CG_TT

Cuts at:   4386    4386 
   Size:       4546
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    899     952    2369    2801    3550    3818     899 
   Size:         53    1417     432     749     268    1627

  Fragments arranged by size: 

                1627    1417     749     432     268      53
 
AflII C'TTAA_G

Cuts at:    592     592 
   Size:       4546
 
AflIII A'CryG_T

Cuts at:   1896    3002    3979    1896 
   Size:       1106     977    2463

  Fragments arranged by size: 

                2463    1106     977
 
AgeI A'CCGG_T

Cuts at:   2934    2934 
   Size:       4546
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1364    1364 
   Size:       4546
 
AlwNI CAG_nnn'CTG

Cuts at:   1317    1849    3418    1317 
   Size:        532    1569    2445

  Fragments arranged by size: 

                2445    1569     532
 
ApaLI G'TGCA_C

Cuts at:   3316    3316 
   Size:       4546
 
ApoI r'AATT_y

Cuts at:    214     700    1548    3899    4155     214 
   Size:        486     848    2351     256     605

  Fragments arranged by size: 

                2351     848     605     486     256
 
AseI AT'TA_AT

Cuts at:     20     637      20 
   Size:        617    3929
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4546
 
AvaI C'yCGr_G

Cuts at:   1564    1564 
   Size:       4546
 
BamHI G'GATC_C

Cuts at:   1569    1569 
   Size:       4546
 
BanI G'GyrC_C

Cuts at:   1560    1974    2222    2257    1560 
   Size:        414     248      35    3849

  Fragments arranged by size: 

                3849     414     248      35
 
BanII G_rGCy'C

Cuts at:   1335    1558    2588    1335 
   Size:        223    1030    3293

  Fragments arranged by size: 

                3293    1030     223
 
BbeI G_GCGC'C

Cuts at:   1978    2226    1978 
   Size:        248    4298
 
BbsI GAAGACnn'nnnn_

Cuts at:    812    1426     812 
   Size:        614    3932
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    135     169     697     731     135 
   Size:         34     528      34    3950

  Fragments arranged by size: 

                3950     528      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2442    3205    4546    2442 
   Size:        763    1341    2442

  Fragments arranged by size: 

                2442    1341     763
 
BclI T'GATC_A

Cuts at:    674    2064     674 
   Size:       1390    3156
 
BfrBI ATG'CAT

Cuts at:    985     985 
   Size:       4546
 
BglII A'GATC_T

Cuts at:    579    2059    4098     579 
   Size:       1480    2039    1027

  Fragments arranged by size: 

                2039    1480    1027
 
BlpI GC'TnA_GC

Cuts at:   1656    1656 
   Size:       4546
 
Bme1580I G_kGCm'C

Cuts at:   2169    2262    3320    2169 
   Size:         93    1058    3395

  Fragments arranged by size: 

                3395    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1756    1837    2172    4318    1756 
   Size:         81     335    2146    1984

  Fragments arranged by size: 

                2146    1984     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    259    2911     259 
   Size:       2652    1894
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    412    1234    1285     412 
   Size:        822      51    3673

  Fragments arranged by size: 

                3673     822      51
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    835    1321    1720    2599    3093    3391     835 
   Size:        486     399     879     494     298    1990

  Fragments arranged by size: 

                1990     879     494     486     399     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    190    4250     190 
   Size:       4060     486
 
BsaAI yAC'GTr

Cuts at:   1536    1897    1536 
   Size:        361    4185
 
BsaBI GATnn'nnATC

Cuts at:      6    1222    2058    4097       6 
   Size:       1216     836    2039     455

  Fragments arranged by size: 

                2039    1216     836     455
 
BsaHI Gr'CG_yC

Cuts at:    139    1975    2223    2927    4423     139 
   Size:       1836     248     704    1496     262

  Fragments arranged by size: 

                1836    1496     704     262     248
 
BsaWI w'CCGG_w

Cuts at:   1733    2254    2934    3208    3355    3766    1733 
   Size:        521     680     274     147     411    2513

  Fragments arranged by size: 

                2513     680     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1030    1030 
   Size:       4546
 
BseYI C'CCAG_C

Cuts at:   1969    2727    3306    3802    1969 
   Size:        758     579     496    2713

  Fragments arranged by size: 

                2713     758     579     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1523    1523 
   Size:       4546
 
BsiEI CG_ry'CG

Cuts at:    104     149    2086    2132    3342     104 
   Size:         45    1937      46    1210    1308

  Fragments arranged by size: 

                1937    1308    1210      46      45
 
BsiHKAI G_wGCw'C

Cuts at:    455    1335    1558    2336    2526    3320     455 
   Size:        880     223     778     190     794    1681

  Fragments arranged by size: 

                1681     880     794     778     223     190
 
BsmI GAATG_Cn'

Cuts at:    954     954 
   Size:       4546
 
BsmBI CGTCTCn'nnnn_

Cuts at:    878     878 
   Size:       4546
 
Bsp1286I G_dGCh'C

Cuts at:    455    1335    1558    2169    2262    2336    2526    2588    3320 
   Size:        880     223     611      93      74     190      62     732

Cuts at:   3320     455 
   Size:       1681

  Fragments arranged by size: 

                1681     880     732     611     223     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3766    3766 
   Size:       4546
 
BspHI T'CATG_A

Cuts at:    341     722    3722     341 
   Size:        381    3000    1165

  Fragments arranged by size: 

                3000    1165     381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1594    1808    2110    2491    1594 
   Size:        214     302     381    3649

  Fragments arranged by size: 

                3649     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2836    2890    4376    4541      50 
   Size:       2786      54    1486     165      55

  Fragments arranged by size: 

                2786    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2456    2456 
   Size:       4546
 
BsrFI r'CCGG_y

Cuts at:   2542    2723    2934    2542 
   Size:        181     211    4154

  Fragments arranged by size: 

                4154     211     181
 
BssSI C'ACGA_G

Cuts at:   2815    3175    2815 
   Size:        360    4186
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1921    1921 
   Size:       4546
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4546
 
BstYI r'GATC_y

Cuts at:      1     303     579    1569    2036    2059    2394    2640    3654 
   Size:        302     276     990     467      23     335     246    1014

Cuts at:   3654    3740    3752    4098       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1014     990     467     449     346     335     302     276
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    191     227     191 
   Size:         36    4510
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2494    2675    2838    2494 
   Size:        181     163    4202

  Fragments arranged by size: 

                4202     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1787    1787 
   Size:       4546
 
DraI TTT'AAA

Cuts at:   1172    1543    1172 
   Size:        371    4175
 
DraIII CAC_nnn'GTG

Cuts at:   1153    1153 
   Size:       4546
 
DrdI GACnn_nn'nnGTC

Cuts at:   2250    3110    2250 
   Size:        860    3686
 
EaeI y'GGCC_r

Cuts at:    350    2129    2303    2694    2721    4068     350 
   Size:       1779     174     391      27    1347     828

  Fragments arranged by size: 

                1779    1347     828     391     174      27
 
EagI C'GGCC_G

Cuts at:   2129    2129 
   Size:       4546
 
EarI CTCTTCn'nnn_

Cuts at:    609    1172    1310    1409    2567    2777     609 
   Size:        563     138      99    1158     210    2378

  Fragments arranged by size: 

                2378    1158     563     210     138      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2752    3064    3210    4356    2752 
   Size:        312     146    1146    2942

  Fragments arranged by size: 

                2942    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   1333    1556    1333 
   Size:        223    4323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    412     899     952    1234    1285    2369    2801    3550    3818 
   Size:        487      53     282      51    1084     432     749     268

Cuts at:   3818     412 
   Size:       1140

  Fragments arranged by size: 

                1140    1084     749     487     432     282     268      53
                  51
 
EcoO109I rG'GnC_Cy

Cuts at:    517    1114    1683     517 
   Size:        597     569    3380

  Fragments arranged by size: 

                3380     597     569
 
EcoRI G'AATT_C

Cuts at:    700    1548     700 
   Size:        848    3698
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2563    2563 
   Size:       4546
 
FspI TGC'GCA

Cuts at:   2325    2325 
   Size:       4546
 
HaeII r_GCGC'y

Cuts at:   1978    2226    3250    4229    1978 
   Size:        248    1024     979    2295

  Fragments arranged by size: 

                2295    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    253     285     799     831    2386    2416    2418    2448     253 
   Size:         32     514      32    1555      30       2      30    2351

  Fragments arranged by size: 

                2351    1555     514      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1583    1583 
   Size:       4546
 
HindIII A'AGCT_T

Cuts at:    538     668     538 
   Size:        130    4416
 
KasI G'GCGC_C

Cuts at:   1974    2222    1974 
   Size:        248    4298
 
KpnI G_GTAC'C

Cuts at:   1564    1564 
   Size:       4546
 
MluI A'CGCG_T

Cuts at:   3979    3979 
   Size:       4546
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    169     804    1022    3217    3401     169 
   Size:        635     218    2195     184    1314

  Fragments arranged by size: 

                2195    1314     635     218     184
 
MscI TGG'CCA

Cuts at:    352    2305     352 
   Size:       1953    2593
 
MslI CAynn'nnrTG

Cuts at:    346     947    2533    2660     346 
   Size:        601    1586     127    2232

  Fragments arranged by size: 

                2232    1586     601     127
 
MspA1I CmG'CkG

Cuts at:   1317    1655    1969    2329    3344    3589    1317 
   Size:        338     314     360    1015     245    2274

  Fragments arranged by size: 

                2274    1015     360     338     314     245
 
NaeI GCC'GGC

Cuts at:   2725    2725 
   Size:       4546
 
NarI GG'CG_CC

Cuts at:   1975    2223    1975 
   Size:        248    4298
 
NcoI C'CATG_G

Cuts at:    191     227     191 
   Size:         36    4510
 
NgoMIV G'CCGG_C

Cuts at:   2723    2723 
   Size:       4546
 
NruI TCG'CGA

Cuts at:   2907    2907 
   Size:       4546
 
NsiI A_TGCA'T

Cuts at:    987     987 
   Size:       4546
 
NspI r_CATG'y

Cuts at:   1597    2097    3006    1597 
   Size:        500     909    3137

  Fragments arranged by size: 

                3137     909     500
 
PciI A'CATG_T

Cuts at:   3002    3002 
   Size:       4546
 
PfoI T'CCnGG_A

Cuts at:    585     585 
   Size:       4546
 
PmeI GTTT'AAAC

Cuts at:   1543    1543 
   Size:       4546
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3685    3717    3685 
   Size:         32    4514
 
PpuMI rG'GwC_Cy

Cuts at:   1114    1114 
   Size:       4546
 
PstI C_TGCA'G

Cuts at:   1137    1364    1591    2276    1137 
   Size:        227     227     685    3407

  Fragments arranged by size: 

                3407     685     227     227
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4546
 
PvuII CAG'CTG

Cuts at:   1317    1969    2329    1317 
   Size:        652     360    3534

  Fragments arranged by size: 

                3534     652     360
 
RsrII CG'GwC_CG

Cuts at:   2739    2739 
   Size:       4546
 
SacI G_AGCT'C

Cuts at:   1335    1558    1335 
   Size:        223    4323
 
SalI G'TCGA_C

Cuts at:   1581    1581 
   Size:       4546
 
SanDI GG'GwC_CC

Cuts at:   1114    1114 
   Size:       4546
 
SapI GCTCTTCn'nnn_

Cuts at:    609    2567    2777     609 
   Size:       1958     210    2378

  Fragments arranged by size: 

                2378    1958     210
 
SbfI CC_TGCA'GG

Cuts at:   1591    1591 
   Size:       4546
 
ScaI AGT'ACT

Cuts at:   4063    4063 
   Size:       4546
 
SfcI C'TryA_G

Cuts at:     33    1133    1360    1587    2272    3267    3458      33 
   Size:       1100     227     227     685     995     191    1121

  Fragments arranged by size: 

                1121    1100     995     685     227     227     191
 
SfoI GGC'GCC

Cuts at:   1976    2224    1976 
   Size:        248    4298
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4546
 
SmaI CCC'GGG

Cuts at:   1566    1566 
   Size:       4546
 
SmlI C'TyrA_G

Cuts at:    592     850    1336    1699    2614    3108    3370     592 
   Size:        258     486     363     915     494     262    1768

  Fragments arranged by size: 

                1768     915     494     486     363     262     258
 
SnaBI TAC'GTA

Cuts at:   1536    1536 
   Size:       4546
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4546
 
SphI G_CATG'C

Cuts at:   1597    2097    1597 
   Size:        500    4046
 
StyI C'CwwG_G

Cuts at:    191     227    1678    2032     191 
   Size:         36    1451     354    2705

  Fragments arranged by size: 

                2705    1451     354      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    163    2127    2260    2719    2930     163 
   Size:       1964     133     459     211    1779

  Fragments arranged by size: 

                1964    1779     459     211     133
 
TatI w'GTAC_w

Cuts at:   4061    4061 
   Size:       4546
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4063    4095    4063 
   Size:         32    4514
 
Tth111I GACn'n_nGTC

Cuts at:   2341    2341 
   Size:       4546
 
XbaI T'CTAG_A

Cuts at:    522    1575     522 
   Size:       1053    3493
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2039    2039 
   Size:       4546
 
XmaI C'CCGG_G

Cuts at:   1564    1564 
   Size:       4546
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NruI     NsiI     NspI     PciI
     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BglI    BmgBI     BmtI   Bpu10I    BsaXI
    BsiWI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     PacI     PasI    PflMI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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