Restriction map for pF1KSDA0862
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BglI        BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI          513  GCGG                 CCGC(-3/-1)
AflII        1408  CTTAAG               C^TTAAG
Alw44I        920  GTGCAC               G^TGCAC
AlwNI         230  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         920  GTGCAC               G^TGCAC
Asp700I       751  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         277  GAGCTC               G[GA]GC[CT]^C
BciVI        1035  GTATCC               GTATCC(6/5)
BcnI          291  CCGGG                CC^[GC]GG
BfrI         1408  CTTAAG               C^TTAAG
BglII         328  AGATCT               A^GATCT
BlpI          657  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      657  GCTTAGC              GC^T[ACGT]AGC
BsaI         1267  GGTCTC               GGTCTC(1/5)
BsmAI        1268  GTCTC                GTCTC(1/5)
BsmI          662  GCATTC               GAATGC(1/-1)
Bsp1407I      778  TGTACA               T^GTACA
BsrDI         257  GCAATG               GCAATG(2/0)
BsrGI         778  TGTACA               T^GTACA
BstEII       1471  GGTCACC              G^GT[ACGT]ACC
BstPI        1471  GGTCACC              G^GT[ACGT]ACC
BstXI        1323  CCATGGTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         657  GCTTAGC              GC^T[ACGT]AGC
DraII        1689  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          220  CGGCCA               [CT]^GGCC[GA]
EcoNI         756  CCTTCCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1689  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1471  GGTCACC              G^GT[ACGT]ACC
EcoRI         300  GAATTC               G^AATTC
HapII         291  CCGG                 C^CGG
HpaI         1170  GTTAAC               GTT^AAC
HpaII         291  CCGG                 C^CGG
MspI          291  CCGG                 C^CGG
NciI          291  CCGGG                CC^[GC]GG
NspI          653  GCATGC               [GA]CATG^[CT]
PstI         1617  CTGCAG               CTGCA^G
SacI          277  GAGCTC               GAGCT^C
SphI          653  GCATGC               GCATG^C
SspBI         778  TGTACA               T^GTACA
XbaI         1362  TCTAGA               T^CTAGA
XmnI          751  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI         1252  CCTGAGG              CC^T[ACGT]AGG
             1338  CCTTAGG             
AspHI         277  GAGCTC               G[AT]GC[AT]^C
              920  GTGCAC              
BmyI          277  GAGCTC               G[AGT]GC[ACT]^C
              920  GTGCAC              
BsiHKAI       277  GAGCTC               G[AT]GC[AT]^C
              920  GTGCAC              
BsmFI         238  GGGAC                GGGAC(10/14)
              408  GTCCC               
Bsp1286I      277  GAGCTC               G[AGT]GC[ACT]^C
              920  GTGCAC              
BspHI         443  TCATGA               T^CATGA
             1634  TCATGA              
BspMI         690  ACCTGC               ACCTGC(4/8)
             1586  ACCTGC              
BstNI         184  CCAGG                CC^[AT]GG
              235  CCTGG               
Bsu36I       1252  CCTGAGG              CC^T[ACGT]AGG
             1338  CCTTAGG             
DsaI         1323  CCATGG               C^C[GA][CT]GG
             1739  CCATGG              
Eco81I       1252  CCTGAGG              CC^T[ACGT]AGG
             1338  CCTTAGG             
EcoRII        184  CCAGG                ^CC[AT]GG
              235  CCTGG               
HindIII      1315  AAGCTT               A^AGCTT
             1624  AAGCTT              
MvaI          184  CCAGG                CC^[AT]GG
              235  CCTGG               
MwoI          510  GCTGCGGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              653  GCATGCTTAGC         
NcoI         1323  CCATGG               C^CATGG
             1739  CCATGG              
NlaIV        1689  GGGGCC               GG[ACGT]^[ACGT]CC
             1725  GGGTCC              
PflMI         229  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1039  CCAGTGGGTGG         
RcaI          443  TCATGA               T^CATGA
             1634  TCATGA              
SfcI          836  CTATAG               C^T[GA][CT]AG
             1617  CTGCAG              
TaqI          666  TCGA                 T^CGA
             1095  TCGA                
Tsp45I       1472  GTCAC                ^GT[GC]AC
             1661  GTCAC               
TthHB8I       666  TCGA                 T^CGA
             1095  TCGA                
Van91I        229  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1039  CCAGTGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BfaI        BpmI        BsaJI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoT14I     Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        NdeII       NlaIII      
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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