Restriction map for
pF1KSDA0862
(Circular) (Six-base) MAPSORT of: pF1KSDA0862.seq Check: 9055 from: 1 to: 4885
pF1KSDA0862 4885 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 1871 1921 1871
Size: 50 4835
Acc65I G'GTAC_C
Cuts at: 1899 1899
Size: 4885
AclI AA'CG_TT
Cuts at: 4725 4725
Size: 4885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1676 2708 3140 3889 4157 665
Size: 1011 1032 432 749 268 1393
Fragments arranged by size:
1393 1032 1011 749 432 268
AflII C'TTAA_G
Cuts at: 1534 1534
Size: 4885
AflIII A'CryG_T
Cuts at: 2235 3341 4318 2235
Size: 1106 977 2802
Fragments arranged by size:
2802 1106 977
AgeI A'CCGG_T
Cuts at: 3273 3273
Size: 4885
AlwNI CAG_nnn'CTG
Cuts at: 361 2188 3757 361
Size: 1827 1569 1489
Fragments arranged by size:
1827 1569 1489
ApaLI G'TGCA_C
Cuts at: 1046 3655 1046
Size: 2609 2276
ApoI r'AATT_y
Cuts at: 426 591 674 1062 1232 1244 1347 1887 4238
Size: 165 83 388 170 12 103 540 2351
Cuts at: 4238 4494 426
Size: 256 817
Fragments arranged by size:
2351 817 540 388 256 170 165 103
83 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4885
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4885
AvaI C'yCGr_G
Cuts at: 1903 1903
Size: 4885
BamHI G'GATC_C
Cuts at: 1908 1908
Size: 4885
BanI G'GyrC_C
Cuts at: 1899 2313 2561 2596 1899
Size: 414 248 35 4188
Fragments arranged by size:
4188 414 248 35
BanII G_rGCy'C
Cuts at: 407 1897 2927 407
Size: 1490 1030 2365
Fragments arranged by size:
2365 1490 1030
BbeI G_GCGC'C
Cuts at: 2317 2565 2317
Size: 248 4637
BciVI GTATCCnnnnn_n'
Cuts at: 1172 2781 3544 4885 1172
Size: 1609 763 1341 1172
Fragments arranged by size:
1609 1341 1172 763
BclI T'GATC_A
Cuts at: 2403 2403
Size: 4885
BglII A'GATC_T
Cuts at: 454 2398 4437 454
Size: 1944 2039 902
Fragments arranged by size:
2039 1944 902
BlpI GC'TnA_GC
Cuts at: 784 1995 784
Size: 1211 3674
Bme1580I G_kGCm'C
Cuts at: 1050 2508 2601 3659 1050
Size: 1458 93 1058 2276
Fragments arranged by size:
2276 1458 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2176 2511 4657 2095
Size: 81 335 2146 2323
Fragments arranged by size:
2323 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 4885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 279 1236 1693 239
Size: 40 957 457 3431
Fragments arranged by size:
3431 957 457 40
Bpu10I CC'TnA_GC
Cuts at: 1137 1137
Size: 4885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1426 1750 2059 2938 3432 3730 1426
Size: 324 309 879 494 298 2581
Fragments arranged by size:
2581 879 494 324 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 1399 4589 1399
Size: 3190 1695
BsaAI yAC'GTr
Cuts at: 1875 2236 1875
Size: 361 4524
BsaBI GATnn'nnATC
Cuts at: 6 2397 4436 6
Size: 2391 2039 455
Fragments arranged by size:
2391 2039 455
BsaHI Gr'CG_yC
Cuts at: 2314 2562 3266 4762 2314
Size: 248 704 1496 2437
Fragments arranged by size:
2437 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2072 2593 3273 3547 3694 4105 2072
Size: 521 680 274 147 411 2852
Fragments arranged by size:
2852 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 354 540 1810 2308 3066 3645 4141 354
Size: 186 1270 498 758 579 496 1098
Fragments arranged by size:
1270 1098 758 579 498 496 186
BsiEI CG_ry'CG
Cuts at: 104 2425 2471 3681 104
Size: 2321 46 1210 1308
Fragments arranged by size:
2321 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 407 1050 1897 2675 2865 3659 407
Size: 643 847 778 190 794 1633
Fragments arranged by size:
1633 847 794 778 643 190
BsmI GAATG_Cn'
Cuts at: 788 788
Size: 4885
Bsp1286I G_dGCh'C
Cuts at: 407 1050 1897 2508 2601 2675 2865 2927 3659
Size: 643 847 611 93 74 190 62 732
Cuts at: 3659 407
Size: 1633
Fragments arranged by size:
1633 847 732 643 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4105 4105
Size: 4885
BspHI T'CATG_A
Cuts at: 569 1760 4061 569
Size: 1191 2301 1393
Fragments arranged by size:
2301 1393 1191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 825 1721 1933 2147 2449 2830 825
Size: 896 212 214 302 381 2880
Fragments arranged by size:
2880 896 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 3175 3229 4715 4880 50
Size: 3125 54 1486 165 55
Fragments arranged by size:
3125 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 390 2795 390
Size: 2405 2480
BsrFI r'CCGG_y
Cuts at: 2881 3062 3273 2881
Size: 181 211 4493
Fragments arranged by size:
4493 211 181
BsrGI T'GTAC_A
Cuts at: 904 904
Size: 4885
BssSI C'ACGA_G
Cuts at: 3154 3514 3154
Size: 360 4525
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 2260
Size: 4885
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4885
BstEII G'GTnAC_C
Cuts at: 1597 1597
Size: 4885
BstXI CCAn_nnnn'nTGG
Cuts at: 1456 1456
Size: 4885
BstYI r'GATC_y
Cuts at: 1 168 454 1373 1485 1908 2375 2398 2733
Size: 167 286 919 112 423 467 23 335
Cuts at: 2733 2979 3993 4079 4091 4437 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 919 467 449 423 346 335 286
246 167 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1379 1465 1379
Size: 86 4799
BtgI C'CryG_G
Cuts at: 1449 1865 1449
Size: 416 4469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2833 3014 3177 2833
Size: 181 163 4541
Fragments arranged by size:
4541 181 163
BtsI GCAGTG_nn'
Cuts at: 1050 2126 1050
Size: 1076 3809
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4885
DrdI GACnn_nn'nnGTC
Cuts at: 2589 3449 2589
Size: 860 4025
EaeI y'GGCC_r
Cuts at: 346 2468 2642 3033 3060 4407 346
Size: 2122 174 391 27 1347 824
Fragments arranged by size:
2122 1347 824 391 174 27
EagI C'GGCC_G
Cuts at: 2468 2468
Size: 4885
EarI CTCTTCn'nnn_
Cuts at: 416 1487 1586 2906 3116 416
Size: 1071 99 1320 210 2185
Fragments arranged by size:
2185 1320 1071 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3091 3403 3549 4695 3091
Size: 312 146 1146 3281
Fragments arranged by size:
3281 1146 312 146
EcoICRI GAG'CTC
Cuts at: 405 1895 405
Size: 1490 3395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 279 665 1236 1676 1693 2708 3140 3889
Size: 40 386 571 440 17 1015 432 749
Cuts at: 3889 4157 239
Size: 268 967
Fragments arranged by size:
1015 967 749 571 440 432 386 268
40 17
EcoNI CCTnn'n_nnAGG
Cuts at: 886 886
Size: 4885
EcoO109I rG'GnC_Cy
Cuts at: 1816 2022 1816
Size: 206 4679
EcoRI G'AATT_C
Cuts at: 426 1887 426
Size: 1461 3424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4885
FspI TGC'GCA
Cuts at: 2664 2664
Size: 4885
HaeII r_GCGC'y
Cuts at: 2317 2565 3589 4568 2317
Size: 248 1024 979 2634
Fragments arranged by size:
2634 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 564 596 1372 1404 1836 1868 2725 2755 2757
Size: 32 776 32 432 32 857 30 2
Cuts at: 2757 2787 564
Size: 30 2662
Fragments arranged by size:
2662 857 776 432 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 330 491 1298 1574 1922 330
Size: 161 807 276 348 3293
Fragments arranged by size:
3293 807 348 276 161
HindIII A'AGCT_T
Cuts at: 1441 1750 1441
Size: 309 4576
HpaI GTT'AAC
Cuts at: 1298 1298
Size: 4885
KasI G'GCGC_C
Cuts at: 2313 2561 2313
Size: 248 4637
KpnI G_GTAC'C
Cuts at: 1903 1903
Size: 4885
MluI A'CGCG_T
Cuts at: 4318 4318
Size: 4885
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1463 3556 3740 1463
Size: 2093 184 2608
Fragments arranged by size:
2608 2093 184
MscI TGG'CCA
Cuts at: 2644 2644
Size: 4885
MslI CAynn'nnrTG
Cuts at: 2872 2999 2872
Size: 127 4758
MspA1I CmG'CkG
Cuts at: 1994 2308 2668 3683 3928 1994
Size: 314 360 1015 245 2951
Fragments arranged by size:
2951 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 4885
NarI GG'CG_CC
Cuts at: 2314 2562 2314
Size: 248 4637
NcoI C'CATG_G
Cuts at: 1449 1865 1449
Size: 416 4469
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 4885
NruI TCG'CGA
Cuts at: 3246 3246
Size: 4885
NspI r_CATG'y
Cuts at: 783 1936 2436 3345 783
Size: 1153 500 909 2323
Fragments arranged by size:
2323 1153 909 500
PciI A'CATG_T
Cuts at: 3341 3341
Size: 4885
PflMI CCAn_nnn'nTGG
Cuts at: 361 1171 361
Size: 810 4075
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4024 4056 4024
Size: 32 4853
PstI C_TGCA'G
Cuts at: 1747 1930 2615 1747
Size: 183 685 4017
Fragments arranged by size:
4017 685 183
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4885
PvuII CAG'CTG
Cuts at: 2308 2668 2308
Size: 360 4525
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4885
SacI G_AGCT'C
Cuts at: 407 1897 407
Size: 1490 3395
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4885
SapI GCTCTTCn'nnn_
Cuts at: 2906 3116 2906
Size: 210 4675
SbfI CC_TGCA'GG
Cuts at: 1930 1930
Size: 4885
ScaI AGT'ACT
Cuts at: 4402 4402
Size: 4885
SfcI C'TryA_G
Cuts at: 33 962 1743 1926 2611 3606 3797 33
Size: 929 781 183 685 995 191 1121
Fragments arranged by size:
1121 995 929 781 685 191 183
SfoI GGC'GCC
Cuts at: 2315 2563 2315
Size: 248 4637
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4885
SmaI CCC'GGG
Cuts at: 1905 1905
Size: 4885
SmlI C'TyrA_G
Cuts at: 1405 1534 1729 2038 2953 3447 3709 1405
Size: 129 195 309 915 494 262 2581
Fragments arranged by size:
2581 915 494 309 262 195 129
SnaBI TAC'GTA
Cuts at: 1875 1875
Size: 4885
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4885
SphI G_CATG'C
Cuts at: 783 1936 2436 783
Size: 1153 500 3232
Fragments arranged by size:
3232 1153 500
StyI C'CwwG_G
Cuts at: 188 1310 1449 1626 1865 2017 2371 188
Size: 1122 139 177 239 152 354 2702
Fragments arranged by size:
2702 1122 354 239 177 152 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1159 2466 2599 3058 3269 1159
Size: 1307 133 459 211 2775
Fragments arranged by size:
2775 1307 459 211 133
TatI w'GTAC_w
Cuts at: 904 4400 904
Size: 3496 1389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4402 4434 4402
Size: 32 4853
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4885
XbaI T'CTAG_A
Cuts at: 1488 1914 1488
Size: 426 4459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 2378
Size: 4885
XmaI C'CCGG_G
Cuts at: 1903 1903
Size: 4885
XmnI GAAnn'nnTTC
Cuts at: 881 881
Size: 4885
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI MfeI
NdeI NheI NotI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI