Restriction map for pF1KSDA0860
 (Circular) (Six-base) MAPSORT of: pF1KM.seq  Check: 3141  from: 1  to: 3452




 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AatII G_ACGT'C

Cuts at:    245     245 
   Size:       3452
 
AccI GT'mk_AC

Cuts at:    488     488 
   Size:       3452
 
Acc65I G'GTAC_C

Cuts at:    466     466 
   Size:       3452
 
AclI AA'CG_TT

Cuts at:   3292    3292 
   Size:       3452
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     339    1275    1707    2456    2724     136 
   Size:        203     936     432     749     268     864

  Fragments arranged by size: 

                 936     864     749     432     268     203
 
AflIII A'CryG_T

Cuts at:    125     802    1908    2885     125 
   Size:        677    1106     977     692

  Fragments arranged by size: 

                1106     977     692     677
 
AgeI A'CCGG_T

Cuts at:   1840    1840 
   Size:       3452
 
AlwNI CAG_nnn'CTG

Cuts at:    755    2324     755 
   Size:       1569    1883
 
ApaLI G'TGCA_C

Cuts at:   2222    2222 
   Size:       3452
 
ApoI r'AATT_y

Cuts at:    360     454    2805    3061     360 
   Size:         94    2351     256     751

  Fragments arranged by size: 

                2351     751     256      94
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3452
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3452
 
AvaI C'yCGr_G

Cuts at:    470     470 
   Size:       3452
 
BamHI G'GATC_C

Cuts at:    475     475 
   Size:       3452
 
BanI G'GyrC_C

Cuts at:    466     880    1128    1163     466 
   Size:        414     248      35    2755

  Fragments arranged by size: 

                2755     414     248      35
 
BanII G_rGCy'C

Cuts at:    464    1494     464 
   Size:       1030    2422
 
BbeI G_GCGC'C

Cuts at:    884    1132     884 
   Size:        248    3204
 
BciVI GTATCCnnnnn_n'

Cuts at:   1348    2111    3452    1348 
   Size:        763    1341    1348

  Fragments arranged by size: 

                1348    1341     763
 
BclI T'GATC_A

Cuts at:    970     970 
   Size:       3452
 
BglII A'GATC_T

Cuts at:    965    3004     965 
   Size:       2039    1413
 
BlpI GC'TnA_GC

Cuts at:    562     562 
   Size:       3452
 
Bme1580I G_kGCm'C

Cuts at:   1075    1168    2226    1075 
   Size:         93    1058    2301

  Fragments arranged by size: 

                2301    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    662     743    1078    3224     662 
   Size:         81     335    2146     890

  Fragments arranged by size: 

                2146     890     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1817    1817 
   Size:       3452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    283     283 
   Size:       3452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    366     626    1505    1999    2297     366 
   Size:        260     879     494     298    1521

  Fragments arranged by size: 

                1521     879     494     298     260
 
BsaI GGTCTCn'nnnn_

Cuts at:   3156    3156 
   Size:       3452
 
BsaAI yAC'GTr

Cuts at:    803     803 
   Size:       3452
 
BsaBI GATnn'nnATC

Cuts at:      6     964    3003       6 
   Size:        958    2039     455

  Fragments arranged by size: 

                2039     958     455
 
BsaHI Gr'CG_yC

Cuts at:    242     881    1129    1833    3329     242 
   Size:        639     248     704    1496     365

  Fragments arranged by size: 

                1496     704     639     365     248
 
BsaWI w'CCGG_w

Cuts at:    368     639    1160    1840    2114    2261    2672     368 
   Size:        271     521     680     274     147     411    1148

  Fragments arranged by size: 

                1148     680     521     411     274     271     147
 
BseYI C'CCAG_C

Cuts at:    212     875    1633    2212    2708     212 
   Size:        663     758     579     496     956

  Fragments arranged by size: 

                 956     758     663     579     496
 
BsiEI CG_ry'CG

Cuts at:    104     992    1038    2248     104 
   Size:        888      46    1210    1308

  Fragments arranged by size: 

                1308    1210     888      46
 
BsiHKAI G_wGCw'C

Cuts at:    464    1242    1432    2226     464 
   Size:        778     190     794    1690

  Fragments arranged by size: 

                1690     794     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    464    1075    1168    1242    1432    1494    2226     464 
   Size:        611      93      74     190      62     732    1690

  Fragments arranged by size: 

                1690     732     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2672    2672 
   Size:       3452
 
BspHI T'CATG_A

Cuts at:   2628    2628 
   Size:       3452
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    500     714    1016    1397     500 
   Size:        214     302     381    2555

  Fragments arranged by size: 

                2555     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1742    1796    3282    3447      50 
   Size:       1692      54    1486     165      55

  Fragments arranged by size: 

                1692    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1362    1362 
   Size:       3452
 
BsrFI r'CCGG_y

Cuts at:   1448    1629    1840    1448 
   Size:        181     211    3060

  Fragments arranged by size: 

                3060     211     181
 
BssSI C'ACGA_G

Cuts at:   1721    2081    1721 
   Size:        360    3092
 
BstAPI GCAn_nnn'nTGC

Cuts at:    827     827 
   Size:       3452
 
BstYI r'GATC_y

Cuts at:      1     475     942     965    1300    1546    2560    2646    2658 
   Size:        474     467      23     335     246    1014      86      12

Cuts at:   2658    3004       1 
   Size:        346     449

  Fragments arranged by size: 

                1014     474     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3452
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1400    1581    1744    1400 
   Size:        181     163    3108

  Fragments arranged by size: 

                3108     181     163
 
BtsI GCAGTG_nn'

Cuts at:    693     693 
   Size:       3452
 
DraI TTT'AAA

Cuts at:    449     449 
   Size:       3452
 
DrdI GACnn_nn'nnGTC

Cuts at:   1156    2016    1156 
   Size:        860    2592
 
EaeI y'GGCC_r

Cuts at:   1035    1209    1600    1627    2974    1035 
   Size:        174     391      27    1347    1513

  Fragments arranged by size: 

                1513    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   1035    1035 
   Size:       3452
 
EarI CTCTTCn'nnn_

Cuts at:   1473    1683    1473 
   Size:        210    3242
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    281    1658    1970    2116    3262     281 
   Size:       1377     312     146    1146     471

  Fragments arranged by size: 

                1377    1146     471     312     146
 
EcoICRI GAG'CTC

Cuts at:    462     462 
   Size:       3452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     283     339    1275    1707    2456    2724     136 
   Size:        147      56     936     432     749     268     864

  Fragments arranged by size: 

                 936     864     749     432     268     147      56
 
EcoO109I rG'GnC_Cy

Cuts at:    589     589 
   Size:       3452
 
EcoRI G'AATT_C

Cuts at:    454     454 
   Size:       3452
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    250    1469     250 
   Size:       1219    2233
 
FspI TGC'GCA

Cuts at:   1231    1231 
   Size:       3452
 
HaeII r_GCGC'y

Cuts at:    884    1132    2156    3135     884 
   Size:        248    1024     979    1201

  Fragments arranged by size: 

                1201    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    262     294    1292    1322    1324    1354     262 
   Size:         32     998      30       2      30    2360

  Fragments arranged by size: 

                2360     998      32      30      30       2
 
HincII GTy'rAC

Cuts at:    489     489 
   Size:       3452
 
KasI G'GCGC_C

Cuts at:    880    1128     880 
   Size:        248    3204
 
KpnI G_GTAC'C

Cuts at:    470     470 
   Size:       3452
 
MluI A'CGCG_T

Cuts at:   2885    2885 
   Size:       3452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2123    2307    2123 
   Size:        184    3268
 
MscI TGG'CCA

Cuts at:   1211    1211 
   Size:       3452
 
MslI CAynn'nnrTG

Cuts at:   1439    1566    1439 
   Size:        127    3325
 
MspA1I CmG'CkG

Cuts at:    561     875    1235    2250    2495     561 
   Size:        314     360    1015     245    1518

  Fragments arranged by size: 

                1518    1015     360     314     245
 
NaeI GCC'GGC

Cuts at:   1631    1631 
   Size:       3452
 
NarI GG'CG_CC

Cuts at:    881    1129     881 
   Size:        248    3204
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3452
 
NgoMIV G'CCGG_C

Cuts at:   1629    1629 
   Size:       3452
 
NruI TCG'CGA

Cuts at:   1813    1813 
   Size:       3452
 
NspI r_CATG'y

Cuts at:    503    1003    1912     503 
   Size:        500     909    2043

  Fragments arranged by size: 

                2043     909     500
 
PciI A'CATG_T

Cuts at:   1908    1908 
   Size:       3452
 
PmeI GTTT'AAAC

Cuts at:    449     449 
   Size:       3452
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2591    2623    2591 
   Size:         32    3420
 
PstI C_TGCA'G

Cuts at:    497    1182     497 
   Size:        685    2767
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3452
 
PvuII CAG'CTG

Cuts at:    875    1235     875 
   Size:        360    3092
 
RsrII CG'GwC_CG

Cuts at:   1645    1645 
   Size:       3452
 
SacI G_AGCT'C

Cuts at:    464     464 
   Size:       3452
 
SalI G'TCGA_C

Cuts at:    487     487 
   Size:       3452
 
SapI GCTCTTCn'nnn_

Cuts at:   1473    1683    1473 
   Size:        210    3242
 
SbfI CC_TGCA'GG

Cuts at:    497     497 
   Size:       3452
 
ScaI AGT'ACT

Cuts at:   2969    2969 
   Size:       3452
 
SfcI C'TryA_G

Cuts at:     33     493    1178    2173    2364      33 
   Size:        460     685     995     191    1121

  Fragments arranged by size: 

                1121     995     685     460     191
 
SfoI GGC'GCC

Cuts at:    882    1130     882 
   Size:        248    3204
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3452
 
SmaI CCC'GGG

Cuts at:    472     472 
   Size:       3452
 
SmlI C'TyrA_G

Cuts at:    381     605    1520    2014    2276     381 
   Size:        224     915     494     262    1557

  Fragments arranged by size: 

                1557     915     494     262     224
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3452
 
SphI G_CATG'C

Cuts at:    503    1003     503 
   Size:        500    2952
 
StyI C'CwwG_G

Cuts at:    107     584     938     107 
   Size:        477     354    2621

  Fragments arranged by size: 

                2621     477     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    204    1033    1166    1625    1836     204 
   Size:        829     133     459     211    1820

  Fragments arranged by size: 

                1820     829     459     211     133
 
TatI w'GTAC_w

Cuts at:   2967    2967 
   Size:       3452
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2969    3001    2969 
   Size:         32    3420
 
Tth111I GACn'n_nGTC

Cuts at:   1247    1247 
   Size:       3452
 
XbaI T'CTAG_A

Cuts at:    481     481 
   Size:       3452
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    945     945 
   Size:       3452
 
XmaI C'CCGG_G

Cuts at:    470     470 
   Size:       3452
 
ZraI GAC'GTC

Cuts at:    243     243 
   Size:       3452
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NruI     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI
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