(Circular) (Six-base) MAPSORT of: pF1KSDA0831.seq Check: 3885 from: 1 to: 4276 pF1KSDA0831 4276 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1186 1186 Size: 4276 AccI GT'mk_AC Cuts at: 1312 1312 Size: 4276 Acc65I G'GTAC_C Cuts at: 1290 1290 Size: 4276 AclI AA'CG_TT Cuts at: 4116 4116 Size: 4276 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 221 437 521 2099 2531 3280 3548 221 Size: 216 84 1578 432 749 268 949 Fragments arranged by size: 1578 949 749 432 268 216 84 AflII C'TTAA_G Cuts at: 333 333 Size: 4276 AflIII A'CryG_T Cuts at: 1626 2732 3709 1626 Size: 1106 977 2193 Fragments arranged by size: 2193 1106 977 AgeI A'CCGG_T Cuts at: 2664 2664 Size: 4276 AhdI GACnn_n'nnGTC Cuts at: 535 535 Size: 4276 AlwNI CAG_nnn'CTG Cuts at: 1579 3148 1579 Size: 1569 2707 ApaI G_GGCC'C Cuts at: 968 968 Size: 4276 ApaLI G'TGCA_C Cuts at: 3046 3046 Size: 4276 ApoI r'AATT_y Cuts at: 195 784 814 879 1003 1278 3629 3885 195 Size: 589 30 65 124 275 2351 256 586 Fragments arranged by size: 2351 589 586 275 256 124 65 30 AseI AT'TA_AT Cuts at: 20 20 Size: 4276 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4276 AvaI C'yCGr_G Cuts at: 821 1294 821 Size: 473 3803 AvrII C'CTAG_G Cuts at: 951 951 Size: 4276 BamHI G'GATC_C Cuts at: 1011 1299 1011 Size: 288 3988 BanI G'GyrC_C Cuts at: 257 1290 1704 1952 1987 257 Size: 1033 414 248 35 2546 Fragments arranged by size: 2546 1033 414 248 35 BanII G_rGCy'C Cuts at: 389 968 1288 2318 389 Size: 579 320 1030 2347 Fragments arranged by size: 2347 1030 579 320 BbeI G_GCGC'C Cuts at: 1708 1956 1708 Size: 248 4028 BbsI GAAGACnn'nnnn_ Cuts at: 443 443 Size: 4276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1205 1239 1205 Size: 34 4242 BciVI GTATCCnnnnn_n' Cuts at: 2172 2935 4276 2172 Size: 763 1341 2172 Fragments arranged by size: 2172 1341 763 BclI T'GATC_A Cuts at: 1794 1794 Size: 4276 BglII A'GATC_T Cuts at: 1789 3828 1789 Size: 2039 2237 BlpI GC'TnA_GC Cuts at: 1386 1386 Size: 4276 Bme1580I G_kGCm'C Cuts at: 968 1084 1899 1992 3050 968 Size: 116 815 93 1058 2194 Fragments arranged by size: 2194 1058 815 116 93 BmrI ACTGGGnnnn_n' Cuts at: 1098 1125 1486 1567 1902 4048 1098 Size: 27 361 81 335 2146 1326 Fragments arranged by size: 2146 1326 361 335 81 27 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 408 2641 408 Size: 2233 2043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 462 1046 462 Size: 584 3692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1009 1450 2329 2823 3121 1009 Size: 441 879 494 298 2164 Fragments arranged by size: 2164 879 494 441 298 BsaI GGTCTCn'nnnn_ Cuts at: 428 3980 428 Size: 3552 724 BsaAI yAC'GTr Cuts at: 1266 1627 1266 Size: 361 3915 BsaBI GATnn'nnATC Cuts at: 6 755 1788 3827 6 Size: 749 1033 2039 455 Fragments arranged by size: 2039 1033 749 455 BsaHI Gr'CG_yC Cuts at: 1168 1705 1953 2657 4153 1168 Size: 537 248 704 1496 1291 Fragments arranged by size: 1496 1291 704 537 248 BsaWI w'CCGG_w Cuts at: 1463 1984 2664 2938 3085 3496 1463 Size: 521 680 274 147 411 2243 Fragments arranged by size: 2243 680 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 652 1006 1200 652 Size: 354 194 3728 Fragments arranged by size: 3728 354 194 BseYI C'CCAG_C Cuts at: 629 1699 2457 3036 3532 629 Size: 1070 758 579 496 1373 Fragments arranged by size: 1373 1070 758 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1214 1214 Size: 4276 BsiEI CG_ry'CG Cuts at: 104 1816 1862 3072 104 Size: 1712 46 1210 1308 Fragments arranged by size: 1712 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 252 389 1162 1288 2066 2256 3050 252 Size: 137 773 126 778 190 794 1478 Fragments arranged by size: 1478 794 778 773 190 137 126 BsmI GAATG_Cn' Cuts at: 846 846 Size: 4276 Bsp1286I G_dGCh'C Cuts at: 252 389 968 1084 1162 1288 1899 1992 2066 Size: 137 579 116 78 126 611 93 74 Cuts at: 2066 2256 2318 3050 252 Size: 190 62 732 1478 Fragments arranged by size: 1478 732 611 579 190 137 126 116 93 78 74 62 BspEI T'CCGG_A Cuts at: 3496 3496 Size: 4276 BspHI T'CATG_A Cuts at: 3452 3452 Size: 4276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 947 1186 1324 1538 1840 2221 947 Size: 239 138 214 302 381 3002 Fragments arranged by size: 3002 381 302 239 214 138 BsrBI CCG'CTC Cuts at: 50 1043 2566 2620 4106 4271 50 Size: 993 1523 54 1486 165 55 Fragments arranged by size: 1523 1486 993 165 55 54 BsrDI GCAATG_nn' Cuts at: 2186 2186 Size: 4276 BsrFI r'CCGG_y Cuts at: 2272 2453 2664 2272 Size: 181 211 3884 Fragments arranged by size: 3884 211 181 BssSI C'ACGA_G Cuts at: 2545 2905 2545 Size: 360 3916 BstAPI GCAn_nnn'nTGC Cuts at: 1651 1651 Size: 4276 BstBI TT'CG_AA Cuts at: 110 110 Size: 4276 BstEII G'GTnAC_C Cuts at: 304 1227 304 Size: 923 3353 BstYI r'GATC_y Cuts at: 1 1011 1299 1766 1789 2124 2370 3384 3470 Size: 1010 288 467 23 335 246 1014 86 Cuts at: 3470 3482 3828 1 Size: 12 346 449 Fragments arranged by size: 1014 1010 467 449 346 335 288 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 910 910 Size: 4276 BtgI C'CryG_G Cuts at: 998 998 Size: 4276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1076 1164 2224 2405 2568 1076 Size: 88 1060 181 163 2784 Fragments arranged by size: 2784 1060 181 163 88 BtsI GCAGTG_nn' Cuts at: 833 898 1197 1517 833 Size: 65 299 320 3592 Fragments arranged by size: 3592 320 299 65 DraI TTT'AAA Cuts at: 1242 1273 1242 Size: 31 4245 DrdI GACnn_nn'nnGTC Cuts at: 1980 2840 1980 Size: 860 3416 EaeI y'GGCC_r Cuts at: 1859 2033 2424 2451 3798 1859 Size: 174 391 27 1347 2337 Fragments arranged by size: 2337 1347 391 174 27 EagI C'GGCC_G Cuts at: 1859 1859 Size: 4276 EarI CTCTTCn'nnn_ Cuts at: 2297 2507 2297 Size: 210 4066 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2482 2794 2940 4086 2482 Size: 312 146 1146 2672 Fragments arranged by size: 2672 1146 312 146 EcoICRI GAG'CTC Cuts at: 387 1286 387 Size: 899 3377 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 221 437 462 521 1046 2099 2531 3280 3548 Size: 216 25 59 525 1053 432 749 268 Cuts at: 3548 221 Size: 949 Fragments arranged by size: 1053 949 749 525 432 268 216 59 25 EcoO109I rG'GnC_Cy Cuts at: 580 658 964 965 1413 580 Size: 78 306 1 448 3443 Fragments arranged by size: 3443 448 306 78 1 EcoRI G'AATT_C Cuts at: 1278 1278 Size: 4276 EcoRV GAT'ATC Cuts at: 1122 1122 Size: 4276 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2293 Size: 4276 FspI TGC'GCA Cuts at: 2055 2055 Size: 4276 HaeII r_GCGC'y Cuts at: 1708 1956 2980 3959 1708 Size: 248 1024 979 2025 Fragments arranged by size: 2025 1024 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 276 308 521 553 2116 2146 2148 2178 276 Size: 32 213 32 1563 30 2 30 2374 Fragments arranged by size: 2374 1563 213 32 32 30 30 2 HincII GTy'rAC Cuts at: 729 1313 729 Size: 584 3692 HindIII A'AGCT_T Cuts at: 235 493 1244 235 Size: 258 751 3267 Fragments arranged by size: 3267 751 258 KasI G'GCGC_C Cuts at: 1704 1952 1704 Size: 248 4028 KpnI G_GTAC'C Cuts at: 1294 1294 Size: 4276 MluI A'CGCG_T Cuts at: 3709 3709 Size: 4276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2947 3131 2947 Size: 184 4092 MscI TGG'CCA Cuts at: 2035 2035 Size: 4276 MslI CAynn'nnrTG Cuts at: 2263 2390 2263 Size: 127 4149 MspA1I CmG'CkG Cuts at: 629 723 1385 1699 2059 3074 3319 629 Size: 94 662 314 360 1015 245 1586 Fragments arranged by size: 1586 1015 662 360 314 245 94 NaeI GCC'GGC Cuts at: 2455 2455 Size: 4276 NarI GG'CG_CC Cuts at: 1705 1953 1705 Size: 248 4028 NcoI C'CATG_G Cuts at: 998 998 Size: 4276 NgoMIV G'CCGG_C Cuts at: 2453 2453 Size: 4276 NruI TCG'CGA Cuts at: 1183 2637 1183 Size: 1454 2822 NspI r_CATG'y Cuts at: 877 1327 1827 2736 877 Size: 450 500 909 2417 Fragments arranged by size: 2417 909 500 450 PasI CC'CwG_GG Cuts at: 654 654 Size: 4276 PciI A'CATG_T Cuts at: 2732 2732 Size: 4276 PflMI CCAn_nnn'nTGG Cuts at: 1137 1137 Size: 4276 PfoI T'CCnGG_A Cuts at: 1014 1014 Size: 4276 PmeI GTTT'AAAC Cuts at: 1273 1273 Size: 4276 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3415 3447 3415 Size: 32 4244 PspOMI G'GGCC_C Cuts at: 964 964 Size: 4276 PspXI vC'TCGA_Gb Cuts at: 821 821 Size: 4276 PstI C_TGCA'G Cuts at: 1218 1321 2006 1218 Size: 103 685 3488 Fragments arranged by size: 3488 685 103 PvuI CG_AT'CG Cuts at: 104 104 Size: 4276 PvuII CAG'CTG Cuts at: 629 723 1699 2059 629 Size: 94 976 360 2846 Fragments arranged by size: 2846 976 360 94 RsrII CG'GwC_CG Cuts at: 2469 2469 Size: 4276 SacI G_AGCT'C Cuts at: 389 1288 389 Size: 899 3377 SalI G'TCGA_C Cuts at: 1311 1311 Size: 4276 SapI GCTCTTCn'nnn_ Cuts at: 2297 2507 2297 Size: 210 4066 SbfI CC_TGCA'GG Cuts at: 1321 1321 Size: 4276 ScaI AGT'ACT Cuts at: 3793 3793 Size: 4276 SexAI A'CCwGG_T Cuts at: 308 926 308 Size: 618 3658 SfcI C'TryA_G Cuts at: 33 624 1214 1317 2002 2997 3188 33 Size: 591 590 103 685 995 191 1121 Fragments arranged by size: 1121 995 685 591 590 191 103 SfoI GGC'GCC Cuts at: 1706 1954 1706 Size: 248 4028 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4276 SmaI CCC'GGG Cuts at: 1296 1296 Size: 4276 SmlI C'TyrA_G Cuts at: 333 821 988 1429 2344 2838 3100 333 Size: 488 167 441 915 494 262 1509 Fragments arranged by size: 1509 915 494 488 441 262 167 SnaBI TAC'GTA Cuts at: 1266 1266 Size: 4276 SpeI A'CTAG_T Cuts at: 67 67 Size: 4276 SphI G_CATG'C Cuts at: 1327 1827 1327 Size: 500 3776 SspI AAT'ATT Cuts at: 849 849 Size: 4276 StuI AGG'CCT Cuts at: 206 206 Size: 4276 StyI C'CwwG_G Cuts at: 218 583 951 998 1408 1762 218 Size: 365 368 47 410 354 2732 Fragments arranged by size: 2732 410 368 365 354 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 621 1177 1191 1857 1990 2449 2660 621 Size: 556 14 666 133 459 211 2237 Fragments arranged by size: 2237 666 556 459 211 133 14 TatI w'GTAC_w Cuts at: 3791 3791 Size: 4276 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3793 3825 3793 Size: 32 4244 Tth111I GACn'n_nGTC Cuts at: 2071 2071 Size: 4276 XbaI T'CTAG_A Cuts at: 1305 1305 Size: 4276 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1005 1769 1005 Size: 764 3512 XhoI C'TCGA_G Cuts at: 821 821 Size: 4276 XmaI C'CCGG_G Cuts at: 1294 1294 Size: 4276 XmnI GAAnn'nnTTC Cuts at: 206 206 Size: 4276 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AleI AlfI AloI AscI BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI NotI NsiI PacI PmlI PpuMI PshAI PsiI PsrI SacII SanDI SfiI SgrAI SrfI SwaI ZraI