Restriction map for pF1KSDA0813
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BclI        BglII       BlnI        
BsiWI       BspDI       BspHI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HpaI        KpnI        
KspI        MfeI        MluI        MunI        NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         2340  GTCTAC               GT^[AC][GT]AC
AcsI          480  AAATTT               [GA]^AATT[CT]
AflII        2394  CTTAAG               C^TTAAG
AflIII        365  ACATGT               A^C[GA][CT]GT
Alw44I        855  GTGCAC               G^TGCAC
AocI         4301  CCTCAGG              CC^T[ACGT]AGG
ApaLI         855  GTGCAC               G^TGCAC
ApoI          480  AAATTT               [GA]^AATT[CT]
BbrPI        2967  CACGTG               CAC^GTG
BbsI         2643  GAAGAC               GAAGAC(2/6)
BciVI        1954  GTATCC               GTATCC(6/5)
BfaI         2044  CTAG                 C^TAG
BfrI         2394  CTTAAG               C^TTAAG
BlpI         1460  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1460  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2643  GAAGAC               GAAGAC(2/6)
BsaAI        2967  CACGTG               [CT]AC^GT[GA]
BsaBI        1653  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI          43  CGGCCG               CG[GA][CT]^CG
BspLU11I      365  ACATGT               A^CATGT
Bsu36I       4301  CCTCAGG              CC^T[ACGT]AGG
CelII        1460  GCTCAGC              GC^T[ACGT]AGC
CpoI           38  CGGTCCG              CG^G[AT]CCG
DraI         1681  TTTAAA               TTT^AAA
EagI           43  CGGCCG               C^GGCCG
EclXI          43  CGGCCG               C^GGCCG
Eco52I         43  CGGCCG               C^GGCCG
Eco81I       4301  CCTCAGG              CC^T[ACGT]AGG
HindIII       452  AAGCTT               A^AGCTT
MaeI         2044  CTAG                 C^TAG
MamI         1653  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          607  CATATG               CA^TATG
PmaCI        2967  CACGTG               CAC^GTG
PmlI         2967  CACGTG               CAC^GTG
PshAI        2333  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII          38  CGGTCCG              CG^G[AT]CCG
SacI           49  GAGCTC               GAGCT^C
SapI          711  GCTCTTC              GCTCTTC(1/4)
SbfI         2124  CCTGCAGG             CCTGCA^GG
ScaI         1475  AGTACT               AGT^ACT
SexAI        4403  ACCAGGT              A^CC[AT]GGT
SfiI           35  GGCCGGTCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           13  CCCGGG               CCC^GGG
SphI         3212  GCATGC               GCATG^C
Sse8387I     2124  CCTGCAGG             CCTGCA^GG
XmaCI          13  CCCGGG               C^CCGGG
XmaI           13  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccIII       1811  TCCGGA               T^CCGGA
             3144  TCCGGA              
Asp700I      1314  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2310  GAACCAGTTC          
AspI         1528  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4384  GACCCTGTC           
BamHI         224  GGATCC               G^GATCC
             3602  GGATCC              
BcgI         3627  CGACCACATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              866  GCAATGGCATCG        
BsaI          925  GAGACC               GGTCTC(1/5)
             3579  GGTCTC              
BseAI        1811  TCCGGA               T^CCGGA
             3144  TCCGGA              
BsmBI        1378  GAGACG               CGTCTC(1/5)
             3715  GAGACG              
BspEI        1811  TCCGGA               T^CCGGA
             3144  TCCGGA              
DrdI         2224  GACACCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2565  GACCTCCCTGTC        
EarI          712  CTCTTC               CTCTTC(1/4)
              771  GAAGAG              
Ksp632I       712  CTCTTC               CTCTTC(1/4)
              771  GAAGAG              
MroI         1811  TCCGGA               T^CCGGA
             3144  TCCGGA              
PflFI        1528  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4384  GACCCTGTC           
Tth111I      1528  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4384  GACCCTGTC           
XcmI         4086  CCACTGCGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4203  CCAGTGCCAGGATGG     
XmnI         1314  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2310  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       AviII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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