Restriction map for pF1KSDA0782
 (Circular) (Six-base) MAPSORT of: pF1KSDA0782.seq  Check: 7229  from: 1  to: 6754



pF1KSDA0782  6754 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    467    3466     467 
   Size:       2999    3755
 
AccI GT'mk_AC

Cuts at:    988    3422    3740    3790     988 
   Size:       2434     318      50    3952

  Fragments arranged by size: 

                3952    2434     318      50
 
Acc65I G'GTAC_C

Cuts at:   2588    3512    3768    2588 
   Size:        924     256    5574

  Fragments arranged by size: 

                5574     924     256
 
AclI AA'CG_TT

Cuts at:   6594    6594 
   Size:       6754
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    894    1180    4577    5009    5758    6026     894 
   Size:        286    3397     432     749     268    1622

  Fragments arranged by size: 

                3397    1622     749     432     286     268
 
AfeI AGC'GCT

Cuts at:    499     678    1105    2488     499 
   Size:        179     427    1383    4765

  Fragments arranged by size: 

                4765    1383     427     179
 
AflIII A'CryG_T

Cuts at:   3609    4104    5210    6187    3609 
   Size:        495    1106     977    4176

  Fragments arranged by size: 

                4176    1106     977     495
 
AgeI A'CCGG_T

Cuts at:   5142    5142 
   Size:       6754
 
AhdI GACnn_n'nnGTC

Cuts at:   2268    3720    2268 
   Size:       1452    5302
 
AleI CACnn'nnGTG

Cuts at:   1558    3475    1558 
   Size:       1917    4837
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3337    3337 
   Size:       6754
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2023    2055    2023 
   Size:         32    6722
 
AlwNI CAG_nnn'CTG

Cuts at:    630    1037    1521    2157    2400    2950    3317    3628    4057 
   Size:        407     484     636     243     550     367     311     429

Cuts at:   4057    5626     630 
   Size:       1569    1758

  Fragments arranged by size: 

                1758    1569     636     550     484     429     407     367
                 311     243
 
ApaI G_GGCC'C

Cuts at:    666    1686    2028    2087    2726    3369     666 
   Size:       1020     342      59     639     643    4051

  Fragments arranged by size: 

                4051    1020     643     639     342      59
 
ApaLI G'TGCA_C

Cuts at:   2434    2698    5524    2434 
   Size:        264    2826    3664

  Fragments arranged by size: 

                3664    2826     264
 
ApoI r'AATT_y

Cuts at:   1531    3756    6107    6363    1531 
   Size:       2225    2351     256    1922

  Fragments arranged by size: 

                2351    2225    1922     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6754
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6754
 
AvaI C'yCGr_G

Cuts at:    659    1464    1685    1967    3617    3772     659 
   Size:        805     221     282    1650     155    3641

  Fragments arranged by size: 

                3641    1650     805     282     221     155
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1855    1888    1855 
   Size:         33    6721
 
BamHI G'GATC_C

Cuts at:   3777    3777 
   Size:       6754
 
BanI G'GyrC_C

Cuts at:   1301    1560    2588    3512    3768    4182    4430    4465    1301 
   Size:        259    1028     924     256     414     248      35    3590

  Fragments arranged by size: 

                3590    1028     924     414     259     256     248      35
            
 
BanII G_rGCy'C

Cuts at:    146     355     666    1056    1202    1686    2028    2063    2087 
   Size:        209     311     390     146     484     342      35      24

Cuts at:   2087    2516    2726    2828    2996    3369    3542    3605    3766 
   Size:        429     210     102     168     373     173      63     161

Cuts at:   3766    4796     146 
   Size:       1030    2104

  Fragments arranged by size: 

                2104    1030     484     429     390     373     342     311
                 210     209     173     168     161     146     102      63
                  35      24
 
BbeI G_GCGC'C

Cuts at:   4186    4434    4186 
   Size:        248    6506
 
BbsI GAAGACnn'nnnn_

Cuts at:   2064    2395    2783    2064 
   Size:        331     388    6035

  Fragments arranged by size: 

                6035     388     331
 
BbvCI CC'TCA_GC

Cuts at:    977    1058    2987     977 
   Size:         81    1929    4744

  Fragments arranged by size: 

                4744    1929      81
 
BciVI GTATCCnnnnn_n'

Cuts at:    441    4650    5413    6754     441 
   Size:       4209     763    1341     441

  Fragments arranged by size: 

                4209    1341     763     441
 
BclI T'GATC_A

Cuts at:    453     560    4272     453 
   Size:        107    3712    2935

  Fragments arranged by size: 

                3712    2935     107
 
BglI GCCn_nnn'nGGC

Cuts at:    589    1631    2511    3178    3260     589 
   Size:       1042     880     667      82    4083

  Fragments arranged by size: 

                4083    1042     880     667      82
 
BglII A'GATC_T

Cuts at:   4267    6306    4267 
   Size:       2039    4715
 
BlpI GC'TnA_GC

Cuts at:    190     647    3864     190 
   Size:        457    3217    3080

  Fragments arranged by size: 

                3217    3080     457
 
Bme1580I G_kGCm'C

Cuts at:    660     666    1256    1686    1811    2028    2087    2438    2702 
   Size:          6     590     430     125     217      59     351     264

Cuts at:   2702    2726    3139    3369    4377    4470    5528     660 
   Size:         24     413     230    1008      93    1058    1886

  Fragments arranged by size: 

                1886    1058    1008     590     430     413     351     264
                 230     217     125      93      59      24       6
 
BmrI ACTGGGnnnn_n'

Cuts at:   1077    1253    3964    4045    4380    6526    1077 
   Size:        176    2711      81     335    2146    1305

  Fragments arranged by size: 

                2711    2146    1305     335     176      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2598    5119    2598 
   Size:       2521    4233
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    751    1330    1906    2425    2566    2568    3022     751 
   Size:        579     576     519     141       2     454    4483

  Fragments arranged by size: 

                4483     579     576     519     454     141       2
 
Bpu10I CC'TnA_GC

Cuts at:    292     977    1058    1732    1928    2011    2827    2987     292 
   Size:        685      81     674     196      83     816     160    4059

  Fragments arranged by size: 

                4059     816     685     674     196     160      83      81
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2425    2467    3928    4807    5301    5599    2425 
   Size:         42    1461     879     494     298    3580

  Fragments arranged by size: 

                3580    1461     879     494     298      42
 
BsaI GGTCTCn'nnnn_

Cuts at:   1414    2586    3367    6458    1414 
   Size:       1172     781    3091    1710

  Fragments arranged by size: 

                3091    1710    1172     781
 
BsaAI yAC'GTr

Cuts at:    528     762    2460    3744    4105     528 
   Size:        234    1698    1284     361    3177

  Fragments arranged by size: 

                3177    1698    1284     361     234
 
BsaBI GATnn'nnATC

Cuts at:      6     452    4266    6305       6 
   Size:        446    3814    2039     455

  Fragments arranged by size: 

                3814    2039     455     446
 
BsaHI Gr'CG_yC

Cuts at:    260    1139    1989    4183    4431    5135    6631     260 
   Size:        879     850    2194     248     704    1496     383

  Fragments arranged by size: 

                2194    1496     879     850     704     383     248
 
BsaWI w'CCGG_w

Cuts at:   1547    3341    3941    4462    5142    5416    5563    5974    1547 
   Size:       1794     600     521     680     274     147     411    2327

  Fragments arranged by size: 

                2327    1794     680     600     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    934     964    1057    1087    1303    1333    2023    2053     934 
   Size:         30      93      30     216      30     690      30    5635

  Fragments arranged by size: 

                5635     690     216      93      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    154     820    1046    1384    2584    2587    2857    3004    3091 
   Size:        666     226     338    1200       3     270     147      87

Cuts at:   3091     154 
   Size:       3817

  Fragments arranged by size: 

                3817    1200     666     338     270     226     147      87
                   3
 
BseYI C'CCAG_C

Cuts at:    307     687    1212    1225    1276    1288    1447    1983    2226 
   Size:        380     525      13      51      12     159     536     243

Cuts at:   2226    2528    2894    2970    3132    3461    3639    4177    4935 
   Size:        302     366      76     162     329     178     538     758

Cuts at:   4935    5514    6010     307 
   Size:        579     496    1051

  Fragments arranged by size: 

                1051     758     579     538     536     525     496     380
                 366     329     302     243     178     162     159      76
                  51      13      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    805    1130    2901    3079    3109    3592     805 
   Size:        325    1771     178      30     483    3967

  Fragments arranged by size: 

                3967    1771     483     325     178      30
 
BsiEI CG_ry'CG

Cuts at:    104    1619    3294    4294    4340    5550     104 
   Size:       1515    1675    1000      46    1210    1308

  Fragments arranged by size: 

                1675    1515    1308    1210    1000      46
 
BsiHKAI G_wGCw'C

Cuts at:   1121    1202    1838    2063    2438    2702    2828    2996    3542 
   Size:         81     636     225     375     264     126     168     546

Cuts at:   3542    3766    4544    4734    5528    1121 
   Size:        224     778     190     794    2347

  Fragments arranged by size: 

                2347     794     778     636     546     375     264     225
                 224     190     168     126      81
 
BsmI GAATG_Cn'

Cuts at:   1933    1933 
   Size:       6754
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1146    1146 
   Size:       6754
 
Bsp1286I G_dGCh'C

Cuts at:    146     355     660     666    1056    1121    1202    1256    1686 
   Size:        209     305       6     390      65      81      54     430

Cuts at:   1686    1811    1838    2028    2063    2087    2438    2516    2702 
   Size:        125      27     190      35      24     351      78     186

Cuts at:   2702    2726    2828    2996    3139    3369    3542    3605    3766 
   Size:         24     102     168     143     230     173      63     161

Cuts at:   3766    4377    4470    4544    4734    4796    5528     146 
   Size:        611      93      74     190      62     732    1372

  Fragments arranged by size: 

                1372     732     611     430     390     351     305     230
                 209     190     190     186     173     168     161     143
                 125     102      93      81      78      74      65      63
                  62      54      35      27      24      24       6
 
BspEI T'CCGG_A

Cuts at:   3341    5974    3341 
   Size:       2633    4121
 
BspHI T'CATG_A

Cuts at:   1905    5930    1905 
   Size:       4025    2729
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    467    2510    3061    3466    3802    4016    4318    4699     467 
   Size:       2043     551     405     336     214     302     381    2522

  Fragments arranged by size: 

                2522    2043     551     405     381     336     302     214
            
 
BsrBI CCG'CTC

Cuts at:     50     609    1353    1752    1980    2202    3362    5044    5098 
   Size:        559     744     399     228     222    1160    1682      54

Cuts at:   5098    6584    6749      50 
   Size:       1486     165      55

  Fragments arranged by size: 

                1682    1486    1160     744     559     399     228     222
                 165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4664    4664 
   Size:       6754
 
BsrFI r'CCGG_y

Cuts at:    872    1657    1700    2902    3383    4750    4931    5142     872 
   Size:        785      43    1202     481    1367     181     211    2484

  Fragments arranged by size: 

                2484    1367    1202     785     481     211     181      43
            
 
BsrGI T'GTAC_A

Cuts at:   1850    2186    1850 
   Size:        336    6418
 
BssHII G'CGCG_C

Cuts at:   2425    2425 
   Size:       6754
 
BssSI C'ACGA_G

Cuts at:   5023    5383    5023 
   Size:        360    6394
 
BstAPI GCAn_nnn'nTGC

Cuts at:    630    1521    4129     630 
   Size:        891    2608    3255

  Fragments arranged by size: 

                3255    2608     891
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       6754
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1413    1491    2743    1413 
   Size:         78    1252    5424

  Fragments arranged by size: 

                5424    1252      78
 
BstYI r'GATC_y

Cuts at:      1     412    1956    3005    3777    4244    4267    4602    4848 
   Size:        411    1544    1049     772     467      23     335     246

Cuts at:   4848    5862    5948    5960    6306       1 
   Size:       1014      86      12     346     449

  Fragments arranged by size: 

                1544    1049    1014     772     467     449     411     346
                 335     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1475    3276    3629    1475 
   Size:       1801     353    4600

  Fragments arranged by size: 

                4600    1801     353
 
BtgI C'CryG_G

Cuts at:    163     332     641     767    1122    1824    3173    3355    3710 
   Size:        169     309     126     355     702    1349     182     355

Cuts at:   3710     163 
   Size:       3207

  Fragments arranged by size: 

                3207    1349     702     355     355     309     182     169
                 126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    957    4702    4883    5046     957 
   Size:       3745     181     163    2665

  Fragments arranged by size: 

                3745    2665     181     163
 
BtsI GCAGTG_nn'

Cuts at:    952    1763    1811    2344    2849    2879    3514    3629    3995 
   Size:        811      48     533     505      30     635     115     366

Cuts at:   3995     952 
   Size:       3711

  Fragments arranged by size: 

                3711     811     635     533     505     366     115      48
                  30
 
DraI TTT'AAA

Cuts at:   3751    3751 
   Size:       6754
 
DraIII CAC_nnn'GTG

Cuts at:   2339    2796    3153    2339 
   Size:        457     357    5940

  Fragments arranged by size: 

                5940     457     357
 
DrdI GACnn_nn'nnGTC

Cuts at:   4458    5318    4458 
   Size:        860    5894
 
EaeI y'GGCC_r

Cuts at:    179     764    1001    2763    3197    4337    4511    4902    4929 
   Size:        585     237    1762     434    1140     174     391      27

Cuts at:   4929    6276     179 
   Size:       1347     657

  Fragments arranged by size: 

                1762    1347    1140     657     585     434     391     237
                 174      27
 
EagI C'GGCC_G

Cuts at:   4337    4337 
   Size:       6754
 
EarI CTCTTCn'nnn_

Cuts at:    230    1207    1685    2087    2521    2737    2816    2927    3731 
   Size:        977     478     402     434     216      79     111     804

Cuts at:   3731    4775    4985     230 
   Size:       1044     210    1999

  Fragments arranged by size: 

                1999    1044     977     804     478     434     402     216
                 210     111      79
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    287    4960    5272    5418    6564     287 
   Size:       4673     312     146    1146     477

  Fragments arranged by size: 

                4673    1146     477     312     146
 
EcoICRI GAG'CTC

Cuts at:   1200    2061    2826    2994    3540    3764    1200 
   Size:        861     765     168     546     224    4190

  Fragments arranged by size: 

                4190     861     765     546     224     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    751     894    1180    1330    1906    2425    2566    2568    3022 
   Size:        143     286     150     576     519     141       2     454

Cuts at:   3022    4577    5009    5758    6026     751 
   Size:       1555     432     749     268    1479

  Fragments arranged by size: 

                1555    1479     749     576     519     454     432     286
                 268     150     143     141       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:    634    1951    2416    2443     634 
   Size:       1317     465      27    4945

  Fragments arranged by size: 

                4945    1317     465      27
 
EcoO109I rG'GnC_Cy

Cuts at:    627     981    1266    1383    1470    1479    1728    2083    2084 
   Size:        354     285     117      87       9     249     355       1

Cuts at:   2084    2640    2891    2967    3365    3366    3891     627 
   Size:        556     251      76     398       1     525    3490

  Fragments arranged by size: 

                3490     556     525     398     355     354     285     251
                 249     117      87      76       9       1       1
 
EcoRI G'AATT_C

Cuts at:   1531    3756    1531 
   Size:       2225    4529
 
EcoRV GAT'ATC

Cuts at:   2298    2298 
   Size:       6754
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4771    4771 
   Size:       6754
 
FspI TGC'GCA

Cuts at:   3733    4533    3733 
   Size:        800    5954
 
HaeII r_GCGC'y

Cuts at:    501     680    1107    1136    1226    1432    1707    2482    2490 
   Size:        179     427      29      90     206     275     775       8

Cuts at:   2490    2535    3090    4186    4434    5458    6437     501 
   Size:         45     555    1096     248    1024     979     818

  Fragments arranged by size: 

                1096    1024     979     818     775     555     427     275
                 248     206     179      90      45      29       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    193     225     404     436     475     507     970    1002    1057 
   Size:         32     179      32      39      32     463      32      55

Cuts at:   1057    1089    2254    2286    2413    2445    2458    2490    2997 
   Size:         32    1165      32     127      32      13      32     507

Cuts at:   2997    3029    3706    3738    4594    4624    4626    4656     193 
   Size:         32     677      32     856      30       2      30    2291

  Fragments arranged by size: 

                2291    1165     856     677     507     463     179     127
                  55      39      32      32      32      32      32      32
                  32      32      32      32      30      30      13       2
            
 
HincII GTy'rAC

Cuts at:   2622    3066    3791    2622 
   Size:        444     725    5585

  Fragments arranged by size: 

                5585     725     444
 
KasI G'GCGC_C

Cuts at:   4182    4430    4182 
   Size:        248    6506
 
KpnI G_GTAC'C

Cuts at:   2592    3516    3772    2592 
   Size:        924     256    5574

  Fragments arranged by size: 

                5574     924     256
 
MfeI C'AATT_G

Cuts at:   2711    2711 
   Size:       6754
 
MluI A'CGCG_T

Cuts at:   3609    6187    3609 
   Size:       2578    4176
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    932    2252    5425    5609     932 
   Size:       1320    3173     184    2077

  Fragments arranged by size: 

                3173    2077    1320     184
 
MscI TGG'CCA

Cuts at:    181    3199    4513     181 
   Size:       3018    1314    2422

  Fragments arranged by size: 

                3018    2422    1314
 
MslI CAynn'nnrTG

Cuts at:    251     841     847    1558    2462    2903    3475    3532    4741 
   Size:        590       6     711     904     441     572      57    1209

Cuts at:   4741    4868     251 
   Size:        127    2137

  Fragments arranged by size: 

                2137    1209     904     711     590     572     441     127
                  57       6
 
MspA1I CmG'CkG

Cuts at:   1212    2022    2100    2318    2397    2838    3317    3712    3863 
   Size:        810      78     218      79     441     479     395     151

Cuts at:   3863    4177    4537    5552    5797    1212 
   Size:        314     360    1015     245    2169

  Fragments arranged by size: 

                2169    1015     810     479     441     395     360     314
                 245     218     151      79      78
 
NaeI GCC'GGC

Cuts at:   1659    1702    4933    1659 
   Size:         43    3231    3480

  Fragments arranged by size: 

                3480    3231      43
 
NarI GG'CG_CC

Cuts at:   4183    4431    4183 
   Size:        248    6506
 
NcoI C'CATG_G

Cuts at:    332     641    1824    3173     332 
   Size:        309    1183    1349    3913

  Fragments arranged by size: 

                3913    1349    1183     309
 
NgoMIV G'CCGG_C

Cuts at:   1657    1700    4931    1657 
   Size:         43    3231    3480

  Fragments arranged by size: 

                3480    3231      43
 
NruI TCG'CGA

Cuts at:   5115    5115 
   Size:       6754
 
NspI r_CATG'y

Cuts at:   2902    3805    4305    5214    2902 
   Size:        903     500     909    4442

  Fragments arranged by size: 

                4442     909     903     500
 
PciI A'CATG_T

Cuts at:   5210    5210 
   Size:       6754
 
PflMI CCAn_nnn'nTGG

Cuts at:   2157    2796    3153    3174    3461    2157 
   Size:        639     357      21     287    5450

  Fragments arranged by size: 

                5450     639     357     287      21
 
PfoI T'CCnGG_A

Cuts at:   2075    3008    3542    2075 
   Size:        933     534    5287

  Fragments arranged by size: 

                5287     933     534
 
PmeI GTTT'AAAC

Cuts at:   3751    3751 
   Size:       6754
 
PmlI CAC'GTG

Cuts at:    528    2460     528 
   Size:       1932    4822
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2023    2055    5893    5925    2023 
   Size:         32    3838      32    2852

  Fragments arranged by size: 

                3838    2852      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1470    1728    2891    2967    1470 
   Size:        258    1163      76    5257

  Fragments arranged by size: 

                5257    1163     258      76
 
PspOMI G'GGCC_C

Cuts at:    662    1682    2024    2083    2722    3365     662 
   Size:       1020     342      59     639     643    4051

  Fragments arranged by size: 

                4051    1020     643     639     342      59
 
PstI C_TGCA'G

Cuts at:    635    1023    1274    1398    1526    2120    3799    4484     635 
   Size:        388     251     124     128     594    1679     685    2905

  Fragments arranged by size: 

                2905    1679     685     594     388     251     128     124
            
 
PvuI CG_AT'CG

Cuts at:    104    3294     104 
   Size:       3190    3564
 
PvuII CAG'CTG

Cuts at:   1212    3317    4177    4537    1212 
   Size:       2105     860     360    3429

  Fragments arranged by size: 

                3429    2105     860     360
 
RsrII CG'GwC_CG

Cuts at:   4947    4947 
   Size:       6754
 
SacI G_AGCT'C

Cuts at:   1202    2063    2828    2996    3542    3766    1202 
   Size:        861     765     168     546     224    4190

  Fragments arranged by size: 

                4190     861     765     546     224     168
 
SacII CC_GC'GG

Cuts at:   3713    3713 
   Size:       6754
 
SalI G'TCGA_C

Cuts at:   3789    3789 
   Size:       6754
 
SanDI GG'GwC_CC

Cuts at:   1470    2891    1470 
   Size:       1421    5333
 
SapI GCTCTTCn'nnn_

Cuts at:   1207    2087    2521    2737    2816    4775    4985    1207 
   Size:        880     434     216      79    1959     210    2976

  Fragments arranged by size: 

                2976    1959     880     434     216     210      79
 
SbfI CC_TGCA'GG

Cuts at:   3799    3799 
   Size:       6754
 
ScaI AGT'ACT

Cuts at:   6271    6271 
   Size:       6754
 
SexAI A'CCwGG_T

Cuts at:   2150    3149    2150 
   Size:        999    5755
 
SfcI C'TryA_G

Cuts at:     33     631    1019    1270    1394    1522    1669    2017    2116 
   Size:        598     388     251     124     128     147     348      99

Cuts at:   2116    3795    4480    5475    5666      33 
   Size:       1679     685     995     191    1121

  Fragments arranged by size: 

                1679    1121     995     685     598     388     348     251
                 191     147     128     124      99
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   3178    3178 
   Size:       6754
 
SfoI GGC'GCC

Cuts at:   4184    4432    4184 
   Size:        248    6506
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6754
 
SmaI CCC'GGG

Cuts at:    661    1687    1969    3619    3774     661 
   Size:       1026     282    1650     155    3641

  Fragments arranged by size: 

                3641    1650    1026     282     155
 
SmlI C'TyrA_G

Cuts at:   2440    2482    3907    4822    5316    5578    2440 
   Size:         42    1425     915     494     262    3616

  Fragments arranged by size: 

                3616    1425     915     494     262      42
 
SnaBI TAC'GTA

Cuts at:   3744    3744 
   Size:       6754
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6754
 
SphI G_CATG'C

Cuts at:   2902    3805    4305    2902 
   Size:        903     500    5351

  Fragments arranged by size: 

                5351     903     500
 
SrfI GCCC'GGGC

Cuts at:    661     661 
   Size:       6754
 
StuI AGG'CCT

Cuts at:   2551    2551 
   Size:       6754
 
StyI C'CwwG_G

Cuts at:    222     332     641    1145    1716    1824    3173    3886    4240 
   Size:        110     309     504     571     108    1349     713     354

Cuts at:   4240     222 
   Size:       2736

  Fragments arranged by size: 

                2736    1349     713     571     504     354     309     110
                 108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    426    1837    3468    4335    4468    4927    5138     426 
   Size:       1411    1631     867     133     459     211    2042

  Fragments arranged by size: 

                2042    1631    1411     867     459     211     133
 
TatI w'GTAC_w

Cuts at:   1850    2186    2657    6269    1850 
   Size:        336     471    3612    2335

  Fragments arranged by size: 

                3612    2335     471     336
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6271    6303    6271 
   Size:         32    6722
 
Tth111I GACn'n_nGTC

Cuts at:   1726    4549    1726 
   Size:       2823    3931
 
XbaI T'CTAG_A

Cuts at:    801    3783     801 
   Size:       2982    3772
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    820    1354    1410    3458    4247     820 
   Size:        534      56    2048     789    3327

  Fragments arranged by size: 

                3327    2048     789     534      56
 
XmaI C'CCGG_G

Cuts at:    659    1685    1967    3617    3772     659 
   Size:       1026     282    1650     155    3641

  Fragments arranged by size: 

                3641    1650    1026     282     155
 
XmnI GAAnn'nnTTC

Cuts at:   1234    1535    1234 
   Size:        301    6453
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AscI    AvrII     BcgI    BfrBI    BmgBI     BmtI
    BsiWI   BstEII  BstZ17I     ClaI    CspCI     FseI    FspAI  HindIII
     HpaI     NdeI     NheI     NotI     NsiI     PacI     PasI    PshAI
     PsiI    PspXI     PsrI    SgrAI     SspI     SwaI     XhoI     ZraI
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