Restriction map for pF1KSDA0778
 (Linear) (Six-base) MAPSORT of: KIAA0778.seq  Check: 25  from: 1  to: 3060



KIAA0778.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      39    3060 
   Size:         39    3021
 
AatII G_ACGT'C

Cuts at:      0    2213    3060 
   Size:       2213     847
 
Acc65I G'GTAC_C

Cuts at:      0     486    3060 
   Size:        486    2574
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    2020    3060 
   Size:        118    1902    1040

  Fragments arranged by size: 

                1902    1040     118
 
AflIII A'CryG_T

Cuts at:      0    1163    3060 
   Size:       1163    1897
 
AgeI A'CCGG_T

Cuts at:      0    1538    3060 
   Size:       1538    1522
 
AleI CACnn'nnGTG

Cuts at:      0    2272    3060 
   Size:       2272     788
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     851     883    3060 
   Size:        851      32    2177

  Fragments arranged by size: 

                2177     851      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1795    2577    3060 
   Size:       1795     782     483

  Fragments arranged by size: 

                1795     782     483
 
ApaI G_GGCC'C

Cuts at:      0     224    1521    3060 
   Size:        224    1297    1539

  Fragments arranged by size: 

                1539    1297     224
 
ApaLI G'TGCA_C

Cuts at:      0    1984    3060 
   Size:       1984    1076
 
ApoI r'AATT_y

Cuts at:      0    1699    3060 
   Size:       1699    1361
 
AvaI C'yCGr_G

Cuts at:      0     212     679    1688    1775    2344    3060 
   Size:        212     467    1009      87     569     716

  Fragments arranged by size: 

                1009     716     569     467     212      87
 
BanI G'GyrC_C

Cuts at:      0     486    1129    3060 
   Size:        486     643    1931

  Fragments arranged by size: 

                1931     643     486
 
BanII G_rGCy'C

Cuts at:      0     198     224     457     665    1099    1379    1521    1754 
   Size:        198      26     233     208     434     280     142     233

Cuts at:   1754    2864    3060 
   Size:       1110     196

  Fragments arranged by size: 

                1110     434     280     233     233     208     198     196
                 142      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1281    1487    2814    3060 
   Size:       1281     206    1327     246

  Fragments arranged by size: 

                1327    1281     246     206
 
BbvCI CC'TCA_GC

Cuts at:      0     491    3060 
   Size:        491    2569
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1137    1171    2140    2174    3060 
   Size:       1137      34     969      34     886

  Fragments arranged by size: 

                1137     969     886      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3035    3060 
   Size:       3035      25
 
BclI T'GATC_A

Cuts at:      0     875    1829    3060 
   Size:        875     954    1231

  Fragments arranged by size: 

                1231     954     875
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43     996    2125    2464    2634    3060 
   Size:         43     953    1129     339     170     426

  Fragments arranged by size: 

                1129     953     426     339     170      43
 
Bme1580I G_kGCm'C

Cuts at:      0     224    1132    1521    1988    2401    2431    3060 
   Size:        224     908     389     467     413      30     629

  Fragments arranged by size: 

                 908     629     467     413     389     224      30
 
BmgBI CAC'GTC

Cuts at:      0    1103    3060 
   Size:       1103    1957
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2587    2650    2667    3060 
   Size:       2587      63      17     393

  Fragments arranged by size: 

                2587     393      63      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1329    3060 
   Size:       1329    1731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724     844     967    1096    2704    2887    3060 
   Size:        724     120     123     129    1608     183     173

  Fragments arranged by size: 

                1608     724     183     173     129     123     120
 
Bpu10I CC'TnA_GC

Cuts at:      0     197     491    3060 
   Size:        197     294    2569

  Fragments arranged by size: 

                2569     294     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    2020    3060 
   Size:       1348     672    1040

  Fragments arranged by size: 

                1348    1040     672
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     551     575     700     773     909    1276    3060 
   Size:        551      24     125      73     136     367    1784

  Fragments arranged by size: 

                1784     551     367     136     125      73      24
 
BsaAI yAC'GTr

Cuts at:      0    1244    1500    2741    3060 
   Size:       1244     256    1241     319

  Fragments arranged by size: 

                1244    1241     319     256
 
BsaBI GATnn'nnATC

Cuts at:      0     529    3060 
   Size:        529    2531
 
BsaHI Gr'CG_yC

Cuts at:      0    2210    3060 
   Size:       2210     850
 
BsaWI w'CCGG_w

Cuts at:      0     602     782    1538    2666    3060 
   Size:        602     180     756    1128     394

  Fragments arranged by size: 

                1128     756     602     394     180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     225     255     548     572     578     602    1528    1558 
   Size:        225      30     293      24       6      24     926      30

Cuts at:   1558    2108    2138    2242    2272    2725    2755    2863    2893 
   Size:        550      30     104      30     453      30     108      30

Cuts at:   2893    3060 
   Size:        167

  Fragments arranged by size: 

                 926     550     453     293     225     167     108     104
                  30      30      30      30      30      30      24      24
                   6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2293    2884    2968    3060 
   Size:       2293     591      84      92

  Fragments arranged by size: 

                2293     591      92      84
 
BseYI C'CCAG_C

Cuts at:      0     144    1092    1623    1647    2075    3035    3060 
   Size:        144     948     531      24     428     960      25

  Fragments arranged by size: 

                 960     948     531     428     144      25      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1579    2797    3060 
   Size:       1579    1218     263

  Fragments arranged by size: 

                1579    1218     263
 
BsiHKAI G_wGCw'C

Cuts at:      0     457     920    1379    1546    1988    3060 
   Size:        457     463     459     167     442    1072

  Fragments arranged by size: 

                1072     463     459     457     442     167
 
BsmI GAATG_Cn'

Cuts at:      0    1529    2752    3060 
   Size:       1529    1223     308

  Fragments arranged by size: 

                1529    1223     308
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1081    2075    2217    2866    3060 
   Size:       1081     994     142     649     194

  Fragments arranged by size: 

                1081     994     649     194     142
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     224     457     665     920    1099    1132    1379 
   Size:        198      26     233     208     255     179      33     247

Cuts at:   1379    1521    1546    1754    1988    2401    2431    2864    3060 
   Size:        142      25     208     234     413      30     433     196

  Fragments arranged by size: 

                 433     413     255     247     234     233     208     208
                 198     196     179     142      33      30      26      25
            
 
BspEI T'CCGG_A

Cuts at:      0     602    3060 
   Size:        602    2458
 
BspHI T'CATG_A

Cuts at:      0    2483    3060 
   Size:       2483     577
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      39    3060 
   Size:         39    3021
 
BsrBI CCG'CTC

Cuts at:      0     674    1578    2937    3060 
   Size:        674     904    1359     123

  Fragments arranged by size: 

                1359     904     674     123
 
BsrDI GCAATG_nn'

Cuts at:      0     119     854    1868    1919    2183    2372    3060 
   Size:        119     735    1014      51     264     189     688

  Fragments arranged by size: 

                1014     735     688     264     189     119      51
 
BsrFI r'CCGG_y

Cuts at:      0    1538    3060 
   Size:       1538    1522
 
BssSI C'ACGA_G

Cuts at:      0    1471    3060 
   Size:       1471    1589
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2758    3060 
   Size:       2758     302
 
BstEII G'GTnAC_C

Cuts at:      0    1836    3060 
   Size:       1836    1224
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465     849    1044    1554    1881    2454    2769    3060 
   Size:        465     384     195     510     327     573     315     291

  Fragments arranged by size: 

                 573     510     465     384     327     315     291     195
            
 
BstYI r'GATC_y

Cuts at:      0    1571    2030    2846    3060 
   Size:       1571     459     816     214

  Fragments arranged by size: 

                1571     816     459     214
 
Bsu36I CC'TnA_GG

Cuts at:      0     816     992    3060 
   Size:        816     176    2068

  Fragments arranged by size: 

                2068     816     176
 
BtgI C'CryG_G

Cuts at:      0       6      41     353    1727    2189    3060 
   Size:          6      35     312    1374     462     871

  Fragments arranged by size: 

                1374     871     462     312      35       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2301    3060 
   Size:       2301     759
 
BtsI GCAGTG_nn'

Cuts at:      0     749    2785    3060 
   Size:        749    2036     275

  Fragments arranged by size: 

                2036     749     275
 
DraIII CAC_nnn'GTG

Cuts at:      0    1019    2581    2951    3060 
   Size:       1019    1562     370     109

  Fragments arranged by size: 

                1562    1019     370     109
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1254    3060 
   Size:       1254    1806
 
EaeI y'GGCC_r

Cuts at:      0      15     980    1004    2126    3060 
   Size:         15     965      24    1122     934

  Fragments arranged by size: 

                1122     965     934      24      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     618    3060 
   Size:        618    2442
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     849    2638    3060 
   Size:        849    1789     422

  Fragments arranged by size: 

                1789     849     422
 
EcoICRI GAG'CTC

Cuts at:      0     455    1377    3060 
   Size:        455     922    1683

  Fragments arranged by size: 

                1683     922     455
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     724     844     967    1096    2020    2704    2887 
   Size:        118     606     120     123     129     924     684     183

Cuts at:   2887    3060 
   Size:        173

  Fragments arranged by size: 

                 924     684     606     183     173     129     123     120
                 118
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     172     565    1558    3060 
   Size:        172     393     993    1502

  Fragments arranged by size: 

                1502     993     393     172
 
EcoO109I rG'GnC_Cy

Cuts at:      0     177    1517    1518    3060 
   Size:        177    1340       1    1542

  Fragments arranged by size: 

                1542    1340     177       1
 
EcoRV GAT'ATC

Cuts at:      0    2481    3060 
   Size:       2481     579
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     150    1758    3060 
   Size:        150    1608    1302

  Fragments arranged by size: 

                1608    1302     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     572     604     625     657    1219    1251 
   Size:        352      32     188      32      21      32     562      32

Cuts at:   1251    1528    1560    1755    1787    1996    2028    3060 
   Size:        277      32     195      32     209      32    1032

  Fragments arranged by size: 

                1032     562     352     277     209     195     188      32
                  32      32      32      32      32      32      21
 
HincII GTy'rAC

Cuts at:      0    1660    1956    3060 
   Size:       1660     296    1104

  Fragments arranged by size: 

                1660    1104     296
 
HindIII A'AGCT_T

Cuts at:      0    1735    3060 
   Size:       1735    1325
 
KpnI G_GTAC'C

Cuts at:      0     490    3060 
   Size:        490    2570
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     638    3060 
   Size:        395     243    2422

  Fragments arranged by size: 

                2422     395     243
 
MscI TGG'CCA

Cuts at:      0     982    1006    3060 
   Size:        982      24    2054

  Fragments arranged by size: 

                2054     982      24
 
MslI CAynn'nnrTG

Cuts at:      0     847     976    1315    1555    1759    1825    1942    2092 
   Size:        847     129     339     240     204      66     117     150

Cuts at:   2092    2188    2272    2434    2542    2770    3060 
   Size:         96      84     162     108     228     290

  Fragments arranged by size: 

                 847     339     290     240     228     204     162     150
                 129     117     108      96      84      66
 
MspA1I CmG'CkG

Cuts at:      0     192     406     873     941    1336    1461    2470    2721 
   Size:        192     214     467      68     395     125    1009     251

Cuts at:   2721    3060 
   Size:        339

  Fragments arranged by size: 

                1009     467     395     339     251     214     192     125
                  68
 
NcoI C'CATG_G

Cuts at:      0     353    2189    3060 
   Size:        353    1836     871

  Fragments arranged by size: 

                1836     871     353
 
NspI r_CATG'y

Cuts at:      0    1167    1979    2931    3060 
   Size:       1167     812     952     129

  Fragments arranged by size: 

                1167     952     812     129
 
PciI A'CATG_T

Cuts at:      0    1163    3060 
   Size:       1163    1897
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1503    2577    3060 
   Size:       1503    1074     483

  Fragments arranged by size: 

                1503    1074     483
 
PfoI T'CCnGG_A

Cuts at:      0     509    2864    3060 
   Size:        509    2355     196

  Fragments arranged by size: 

                2355     509     196
 
PmlI CAC'GTG

Cuts at:      0    1500    2741    3060 
   Size:       1500    1241     319

  Fragments arranged by size: 

                1500    1241     319
 
PspOMI G'GGCC_C

Cuts at:      0     220    1517    3060 
   Size:        220    1297    1543

  Fragments arranged by size: 

                1543    1297     220
 
PvuII CAG'CTG

Cuts at:      0     406     873     941    1336    1461    2470    3060 
   Size:        406     467      68     395     125    1009     590

  Fragments arranged by size: 

                1009     590     467     406     395     125      68
 
SacI G_AGCT'C

Cuts at:      0     457    1379    3060 
   Size:        457     922    1681

  Fragments arranged by size: 

                1681     922     457
 
ScaI AGT'ACT

Cuts at:      0      25    3060 
   Size:         25    3035
 
SexAI A'CCwGG_T

Cuts at:      0     560    2947    3060 
   Size:        560    2387     113

  Fragments arranged by size: 

                2387     560     113
 
SfcI C'TryA_G

Cuts at:      0     936    2970    3060 
   Size:        936    2034      90

  Fragments arranged by size: 

                2034     936      90
 
SmaI CCC'GGG

Cuts at:      0    1777    3060 
   Size:       1777    1283
 
SmlI C'TyrA_G

Cuts at:      0     212    1363    2035    3060 
   Size:        212    1151     672    1025

  Fragments arranged by size: 

                1151    1025     672     212
 
SphI G_CATG'C

Cuts at:      0    1979    3060 
   Size:       1979    1081
 
StuI AGG'CCT

Cuts at:      0     821    3060 
   Size:        821    2239
 
StyI C'CwwG_G

Cuts at:      0     134     173     353    1418    1862    1970    2189    2453 
   Size:        134      39     180    1065     444     108     219     264

Cuts at:   2453    3060 
   Size:        607

  Fragments arranged by size: 

                1065     607     444     264     219     180     134     108
                  39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     379     703     855    1153    2569    2902    3027    3060 
   Size:        379     324     152     298    1416     333     125      33

  Fragments arranged by size: 

                1416     379     333     324     298     152     125      33
            
 
TatI w'GTAC_w

Cuts at:      0      23    3060 
   Size:         23    3037
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147     225     257     548     580    2108    2140 
   Size:        115      32      78      32     291      32    1528      32

Cuts at:   2140    2725    2757    3060 
   Size:        585      32     303

  Fragments arranged by size: 

                1528     585     303     291     115      78      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     586    3060 
   Size:        586    2474
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1977    2578    2679    2769    3060 
   Size:       1977     601     101      90     291

  Fragments arranged by size: 

                1977     601     291     101      90
 
XhoI C'TCGA_G

Cuts at:      0     212    3060 
   Size:        212    2848
 
XmaI C'CCGG_G

Cuts at:      0    1775    3060 
   Size:       1775    1285
 
XmnI GAAnn'nnTTC

Cuts at:      0    2620    3060 
   Size:       2620     440
 
ZraI GAC'GTC

Cuts at:      0    2211    3060 
   Size:       2211     849
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI
     PmlI   PspOMI    PvuII     SacI     ScaI    SexAI     SfcI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AhdI     AlfI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BfrBI    BglII     BlpI
     BmtI    BsiEI    BsiWI    BsrGI   BssHII    BstBI  BstZ17I     ClaI
    CspCI     DraI     EagI    EcoRI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
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