(Circular) (Six-base) MAPSORT of: pF1KSDA0736.seq Check: 2338 from: 1 to: 5363 pF1KSDA0736 5363 bp With 182 enzymes: SgfI * June 20, 2008 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2331 2331 Size: 5363 AccI GT'mk_AC Cuts at: 758 1916 2401 758 Size: 1158 485 3720 Fragments arranged by size: 3720 1158 485 Acc65I G'GTAC_C Cuts at: 1373 2379 1373 Size: 1006 4357 AclI AA'CG_TT Cuts at: 5203 5203 Size: 5363 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1267 2338 3188 3620 4367 4635 1267 Size: 1071 850 432 747 268 1995 Fragments arranged by size: 1995 1071 850 747 432 268 AfeI AGC'GCT Cuts at: 702 1381 702 Size: 679 4684 AflIII A'CryG_T Cuts at: 713 1754 2135 2715 3819 4796 713 Size: 1041 381 580 1104 977 1280 Fragments arranged by size: 1280 1104 1041 977 580 381 AgeI A'CCGG_T Cuts at: 3751 3751 Size: 5363 AleI CACnn'nnGTG Cuts at: 1579 1579 Size: 5363 AlwNI CAG_nnn'CTG Cuts at: 442 828 2346 2668 4235 442 Size: 386 1518 322 1567 1570 Fragments arranged by size: 1570 1567 1518 386 322 ApaLI G'TGCA_C Cuts at: 4133 4133 Size: 5363 ApoI r'AATT_y Cuts at: 1425 2367 4716 4972 1425 Size: 942 2349 256 1816 Fragments arranged by size: 2349 1816 942 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5363 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5363 AvaI C'yCGr_G Cuts at: 431 1588 2333 2383 431 Size: 1157 745 50 3411 Fragments arranged by size: 3411 1157 745 50 AvrII C'CTAG_G Cuts at: 1404 1404 Size: 5363 BamHI G'GATC_C Cuts at: 2388 2388 Size: 5363 BanI G'GyrC_C Cuts at: 474 1373 1903 2379 2793 3041 3076 474 Size: 899 530 476 414 248 35 2761 Fragments arranged by size: 2761 899 530 476 414 248 35 BanII G_rGCy'C Cuts at: 1176 1198 2377 3407 1176 Size: 22 1179 1030 3132 Fragments arranged by size: 3132 1179 1030 22 BbeI G_GCGC'C Cuts at: 478 2797 3045 478 Size: 2319 248 2796 Fragments arranged by size: 2796 2319 248 BbsI GAAGACnn'nnnn_ Cuts at: 849 945 1122 849 Size: 96 177 5090 Fragments arranged by size: 5090 177 96 BbvCI CC'TCA_GC Cuts at: 2283 2283 Size: 5363 BciVI GTATCCnnnnn_n' Cuts at: 3261 4022 5363 3261 Size: 761 1341 3261 Fragments arranged by size: 3261 1341 761 BclI T'GATC_A Cuts at: 2883 2883 Size: 5363 BglI GCCn_nnn'nGGC Cuts at: 1171 2099 1171 Size: 928 4435 BglII A'GATC_T Cuts at: 401 2878 4915 401 Size: 2477 2037 849 Fragments arranged by size: 2477 2037 849 BlpI GC'TnA_GC Cuts at: 2475 2475 Size: 5363 Bme1580I G_kGCm'C Cuts at: 2988 3081 4137 2988 Size: 93 1056 4214 Fragments arranged by size: 4214 1056 93 BmgBI CAC'GTC Cuts at: 1884 2136 1884 Size: 252 5111 BmrI ACTGGGnnnn_n' Cuts at: 302 2575 2656 2991 5135 302 Size: 2273 81 335 2144 530 Fragments arranged by size: 2273 2144 530 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 802 3730 802 Size: 2928 2435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 506 1143 1534 2001 506 Size: 637 391 467 3868 Fragments arranged by size: 3868 637 467 391 Bpu10I CC'TnA_GC Cuts at: 182 1289 2283 182 Size: 1107 994 3262 Fragments arranged by size: 3262 1107 994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 522 1419 1672 2539 3418 3910 4208 522 Size: 897 253 867 879 492 298 1677 Fragments arranged by size: 1677 897 879 867 492 298 253 BsaI GGTCTCn'nnnn_ Cuts at: 137 2323 5067 137 Size: 2186 2744 433 Fragments arranged by size: 2744 2186 433 BsaAI yAC'GTr Cuts at: 2355 2716 2355 Size: 361 5002 BsaBI GATnn'nnATC Cuts at: 6 1348 2230 2877 4914 6 Size: 1342 882 647 2037 455 Fragments arranged by size: 2037 1342 882 647 455 BsaHI Gr'CG_yC Cuts at: 475 2794 3042 3744 5240 475 Size: 2319 248 702 1496 598 Fragments arranged by size: 2319 1496 702 598 248 BsaWI w'CCGG_w Cuts at: 2552 3073 3751 4025 4172 4583 2552 Size: 521 678 274 147 411 3332 Fragments arranged by size: 3332 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 810 840 810 Size: 30 5333 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 289 292 341 361 289 Size: 3 49 20 5291 Fragments arranged by size: 5291 49 20 3 BseYI C'CCAG_C Cuts at: 697 810 1382 2220 2788 3546 4123 4619 697 Size: 113 572 838 568 758 577 496 1441 Fragments arranged by size: 1441 838 758 577 572 568 496 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1755 1840 2249 1755 Size: 85 409 4869 Fragments arranged by size: 4869 409 85 BsiEI CG_ry'CG Cuts at: 104 612 2905 2951 4159 104 Size: 508 2293 46 1208 1308 Fragments arranged by size: 2293 1308 1208 508 46 BsiHKAI G_wGCw'C Cuts at: 2377 3155 3345 4137 2377 Size: 778 190 792 3603 Fragments arranged by size: 3603 792 778 190 BsmI GAATG_Cn' Cuts at: 425 2009 2130 2199 425 Size: 1584 121 69 3589 Fragments arranged by size: 3589 1584 121 69 Bsp1286I G_dGCh'C Cuts at: 1176 1198 2377 2988 3081 3155 3345 3407 4137 Size: 22 1179 611 93 74 190 62 730 Cuts at: 4137 1176 Size: 2402 Fragments arranged by size: 2402 1179 730 611 190 93 74 62 22 BspEI T'CCGG_A Cuts at: 4583 4583 Size: 5363 BspHI T'CATG_A Cuts at: 4539 4539 Size: 5363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1242 1404 2331 2413 2627 2929 3310 1242 Size: 162 927 82 214 302 381 3295 Fragments arranged by size: 3295 927 381 302 214 162 82 BsrBI CCG'CTC Cuts at: 50 459 3655 3709 5193 5358 50 Size: 409 3196 54 1484 165 55 Fragments arranged by size: 3196 1484 409 165 55 54 BsrDI GCAATG_nn' Cuts at: 176 2111 3275 176 Size: 1935 1164 2264 Fragments arranged by size: 2264 1935 1164 BsrFI r'CCGG_y Cuts at: 806 3361 3542 3751 806 Size: 2555 181 209 2418 Fragments arranged by size: 2555 2418 209 181 BssSI C'ACGA_G Cuts at: 3634 3992 3634 Size: 358 5005 BstAPI GCAn_nnn'nTGC Cuts at: 828 2346 2740 828 Size: 1518 394 3451 Fragments arranged by size: 3451 1518 394 BstBI TT'CG_AA Cuts at: 110 3724 110 Size: 3614 1749 BstXI CCAn_nnnn'nTGG Cuts at: 213 213 Size: 5363 BstYI r'GATC_y Cuts at: 1 401 1349 1637 2388 2855 2878 3213 3459 Size: 400 948 288 751 467 23 335 246 Cuts at: 3459 4471 4557 4569 4915 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 948 751 467 449 400 346 335 288 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1917 1917 Size: 5363 BtgI C'CryG_G Cuts at: 125 162 606 125 Size: 37 444 4882 Fragments arranged by size: 4882 444 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3313 3494 3657 3313 Size: 181 163 5019 Fragments arranged by size: 5019 181 163 BtsI GCAGTG_nn' Cuts at: 829 1562 1952 2606 829 Size: 733 390 654 3586 Fragments arranged by size: 3586 733 654 390 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2163 2198 2163 Size: 35 5328 DraI TTT'AAA Cuts at: 2362 2362 Size: 5363 DrdI GACnn_nn'nnGTC Cuts at: 3069 3927 3069 Size: 858 4505 EaeI y'GGCC_r Cuts at: 203 603 609 2072 2948 3122 3513 3540 4885 Size: 400 6 1463 876 174 391 27 1345 Cuts at: 4885 203 Size: 681 Fragments arranged by size: 1463 1345 876 681 400 391 174 27 6 EagI C'GGCC_G Cuts at: 609 2948 609 Size: 2339 3024 EarI CTCTTCn'nnn_ Cuts at: 125 907 1715 3386 3596 125 Size: 782 808 1671 210 1892 Fragments arranged by size: 1892 1671 808 782 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1531 3571 3881 4027 5173 1531 Size: 2040 310 146 1146 1721 Fragments arranged by size: 2040 1721 1146 310 146 EcoICRI GAG'CTC Cuts at: 2375 2375 Size: 5363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 506 1143 1267 1534 2001 2338 3188 3620 4367 Size: 637 124 267 467 337 850 432 747 Cuts at: 4367 4635 506 Size: 268 1234 Fragments arranged by size: 1234 850 747 637 467 432 337 268 267 124 EcoO109I rG'GnC_Cy Cuts at: 222 335 517 1367 2502 222 Size: 113 182 850 1135 3083 Fragments arranged by size: 3083 1135 850 182 113 EcoRI G'AATT_C Cuts at: 2367 2367 Size: 5363 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3382 3382 Size: 5363 FspI TGC'GCA Cuts at: 3144 3144 Size: 5363 HaeII r_GCGC'y Cuts at: 478 652 704 1383 2797 3045 4067 5046 478 Size: 174 52 679 1414 248 1022 979 795 Fragments arranged by size: 1414 1022 979 795 679 248 174 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 810 842 926 958 1311 1339 1343 1371 1530 Size: 32 84 32 353 28 4 28 159 Cuts at: 1530 1552 1562 1584 1729 1761 3205 3235 3237 Size: 22 10 22 145 32 1444 30 2 Cuts at: 3237 3267 810 Size: 30 2906 Fragments arranged by size: 2906 1444 353 159 145 84 32 32 32 30 30 28 28 22 22 10 4 2 HincII GTy'rAC Cuts at: 849 2141 2402 849 Size: 1292 261 3810 Fragments arranged by size: 3810 1292 261 KasI G'GCGC_C Cuts at: 474 2793 3041 474 Size: 2319 248 2796 Fragments arranged by size: 2796 2319 248 KpnI G_GTAC'C Cuts at: 1377 2383 1377 Size: 1006 4357 MluI A'CGCG_T Cuts at: 4796 4796 Size: 5363 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 749 755 796 1337 1607 4034 4218 749 Size: 6 41 541 270 2427 184 1894 Fragments arranged by size: 2427 1894 541 270 184 41 6 MscI TGG'CCA Cuts at: 605 3124 605 Size: 2519 2844 MslI CAynn'nnrTG Cuts at: 772 1468 1579 3352 3479 772 Size: 696 111 1773 127 2656 Fragments arranged by size: 2656 1773 696 127 111 MspA1I CmG'CkG Cuts at: 1048 1121 2215 2287 2474 2788 3148 4161 4406 Size: 73 1094 72 187 314 360 1013 245 Cuts at: 4406 1048 Size: 2005 Fragments arranged by size: 2005 1094 1013 360 314 245 187 73 72 NaeI GCC'GGC Cuts at: 3544 3544 Size: 5363 NarI GG'CG_CC Cuts at: 475 2794 3042 475 Size: 2319 248 2796 Fragments arranged by size: 2796 2319 248 NcoI C'CATG_G Cuts at: 125 125 Size: 5363 NgoMIV G'CCGG_C Cuts at: 3542 3542 Size: 5363 NspI r_CATG'y Cuts at: 202 717 744 1020 1272 1609 2416 2916 3823 Size: 515 27 276 252 337 807 500 907 Cuts at: 3823 202 Size: 1742 Fragments arranged by size: 1742 907 807 515 500 337 276 252 27 PasI CC'CwG_GG Cuts at: 523 523 Size: 5363 PciI A'CATG_T Cuts at: 713 3819 713 Size: 3106 2257 PflMI CCAn_nnn'nTGG Cuts at: 1557 1557 Size: 5363 PfoI T'CCnGG_A Cuts at: 236 2119 236 Size: 1883 3480 PmeI GTTT'AAAC Cuts at: 2362 2362 Size: 5363 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1282 1314 4502 4534 1282 Size: 32 3188 32 2111 Fragments arranged by size: 3188 2111 32 32 PpuMI rG'GwC_Cy Cuts at: 335 517 1367 335 Size: 182 850 4331 Fragments arranged by size: 4331 850 182 PstI C_TGCA'G Cuts at: 2351 2410 3095 2351 Size: 59 685 4619 Fragments arranged by size: 4619 685 59 PvuI CG_AT'CG Cuts at: 104 104 Size: 5363 PvuII CAG'CTG Cuts at: 1048 1121 2215 2287 2788 3148 1048 Size: 73 1094 72 501 360 3263 Fragments arranged by size: 3263 1094 501 360 73 72 RsrII CG'GwC_CG Cuts at: 3558 3558 Size: 5363 SacI G_AGCT'C Cuts at: 2377 2377 Size: 5363 SalI G'TCGA_C Cuts at: 2400 2400 Size: 5363 SanDI GG'GwC_CC Cuts at: 335 517 335 Size: 182 5181 SapI GCTCTTCn'nnn_ Cuts at: 3386 3596 3386 Size: 210 5153 SbfI CC_TGCA'GG Cuts at: 2410 2410 Size: 5363 ScaI AGT'ACT Cuts at: 1657 4880 1657 Size: 3223 2140 SexAI A'CCwGG_T Cuts at: 1369 1369 Size: 5363 SfcI C'TryA_G Cuts at: 33 2347 2406 3091 4084 4275 33 Size: 2314 59 685 993 191 1121 Fragments arranged by size: 2314 1121 993 685 191 59 SfoI GGC'GCC Cuts at: 476 2795 3043 476 Size: 2319 248 2796 Fragments arranged by size: 2796 2319 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5363 SmaI CCC'GGG Cuts at: 433 1590 2335 2385 433 Size: 1157 745 50 3411 Fragments arranged by size: 3411 1157 745 50 SmlI C'TyrA_G Cuts at: 501 1398 1687 2518 3433 3925 4187 501 Size: 897 289 831 915 492 262 1677 Fragments arranged by size: 1677 915 897 831 492 289 262 SnaBI TAC'GTA Cuts at: 2355 2355 Size: 5363 SpeI A'CTAG_T Cuts at: 67 67 Size: 5363 SphI G_CATG'C Cuts at: 202 744 2416 2916 202 Size: 542 1672 500 2649 Fragments arranged by size: 2649 1672 542 500 SspI AAT'ATT Cuts at: 247 247 Size: 5363 StuI AGG'CCT Cuts at: 749 1234 749 Size: 485 4878 StyI C'CwwG_G Cuts at: 125 1404 2258 2298 2497 2851 125 Size: 1279 854 40 199 354 2637 Fragments arranged by size: 2637 1279 854 354 199 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1070 1468 2283 2946 3079 3538 3747 1070 Size: 398 815 663 133 459 209 2686 Fragments arranged by size: 2686 815 663 459 398 209 133 TatI w'GTAC_w Cuts at: 1655 1814 1846 4878 1655 Size: 159 32 3032 2140 Fragments arranged by size: 3032 2140 159 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1017 1049 4880 4912 1017 Size: 32 3831 32 1468 Fragments arranged by size: 3831 1468 32 32 Tth111I GACn'n_nGTC Cuts at: 232 3160 232 Size: 2928 2435 XbaI T'CTAG_A Cuts at: 2394 2394 Size: 5363 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2858 2858 Size: 5363 XmaI C'CCGG_G Cuts at: 431 1588 2333 2383 431 Size: 1157 745 50 3411 Fragments arranged by size: 3411 1157 745 50 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AlfI AloI ApaI AscI BaeI BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SrfI SwaI XhoI XmnI ZraI