Restriction map for pF1KSDA0735
 (Linear) (Six-base) MAPSORT of: KIAA0735.seq  Check: 1191  from: 1  to: 2049



KIAA0735.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0     461    2049 
   Size:        461    1588
 
Acc65I G'GTAC_C

Cuts at:      0    1076    2049 
   Size:       1076     973
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     777     901    2049 
   Size:        777     124    1148

  Fragments arranged by size: 

                1148     777     124
 
AfeI AGC'GCT

Cuts at:      0    1084    2049 
   Size:       1084     965
 
AflIII A'CryG_T

Cuts at:      0     416    1395    2049 
   Size:        416     979     654

  Fragments arranged by size: 

                 979     654     416
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1752    2049 
   Size:       1752     297
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1791    2043    2049 
   Size:       1791     252       6

  Fragments arranged by size: 

                1791     252       6
 
ApaI G_GGCC'C

Cuts at:      0    1154    2049 
   Size:       1154     895
 
ApoI r'AATT_y

Cuts at:      0     673    1042    1903    2049 
   Size:        673     369     861     146

  Fragments arranged by size: 

                 861     673     369     146
 
AvaI C'yCGr_G

Cuts at:      0     683    1651    2030    2049 
   Size:        683     968     379      19

  Fragments arranged by size: 

                 968     683     379      19
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     138     171    2049 
   Size:        138      33    1878

  Fragments arranged by size: 

                1878     138      33
 
BamHI G'GATC_C

Cuts at:      0     326    2049 
   Size:        326    1723
 
BanI G'GyrC_C

Cuts at:      0     198     481    1076    1309    1909    2049 
   Size:        198     283     595     233     600     140

  Fragments arranged by size: 

                 600     595     283     233     198     140
 
BanII G_rGCy'C

Cuts at:      0     901    1154    2049 
   Size:        901     253     895

  Fragments arranged by size: 

                 901     895     253
 
BbeI G_GCGC'C

Cuts at:      0     202     485    1313    1913    2049 
   Size:        202     283     828     600     136

  Fragments arranged by size: 

                 828     600     283     202     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131    1445    2049 
   Size:        131    1314     604

  Fragments arranged by size: 

                1314     604     131
 
BbvCI CC'TCA_GC

Cuts at:      0     532    2049 
   Size:        532    1517
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     166    1862    2049 
   Size:        166    1696     187

  Fragments arranged by size: 

                1696     187     166
 
BclI T'GATC_A

Cuts at:      0    1835    2049 
   Size:       1835     214
 
BglI GCCn_nnn'nGGC

Cuts at:      0     187     196     224     358    2049 
   Size:        187       9      28     134    1691

  Fragments arranged by size: 

                1691     187     134      28       9
 
BglII A'GATC_T

Cuts at:      0     131    2049 
   Size:        131    1918
 
Bme1580I G_kGCm'C

Cuts at:      0    1154    2049 
   Size:       1154     895
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      38     395     743    2049 
   Size:         38     357     348    1306

  Fragments arranged by size: 

                1306     357     348      38
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     619     721    2049 
   Size:        619     102    1328

  Fragments arranged by size: 

                1328     619     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     783     846    1579    2049 
   Size:        189     594      63     733     470

  Fragments arranged by size: 

                 733     594     470     189      63
 
Bpu10I CC'TnA_GC

Cuts at:      0     532     980    2049 
   Size:        532     448    1069

  Fragments arranged by size: 

                1069     532     448
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934    1681    2049 
   Size:        934     747     368

  Fragments arranged by size: 

                 934     747     368
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     280    2049 
   Size:        280    1769
 
BsaHI Gr'CG_yC

Cuts at:      0     199     482    1310    1910    2049 
   Size:        199     283     828     600     139

  Fragments arranged by size: 

                 828     600     283     199     139
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     710    2049 
   Size:        277     433    1339

  Fragments arranged by size: 

                1339     433     277
 
BseYI C'CCAG_C

Cuts at:      0     513     714     785    2049 
   Size:        513     201      71    1264

  Fragments arranged by size: 

                1264     513     201      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     604     843    2049 
   Size:        425     179     239    1206

  Fragments arranged by size: 

                1206     425     239     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     645     852    1520    2049 
   Size:        645     207     668     529

  Fragments arranged by size: 

                 668     645     529     207
 
BsmI GAATG_Cn'

Cuts at:      0     449    1201    1827    1883    2049 
   Size:        449     752     626      56     166

  Fragments arranged by size: 

                 752     626     449     166      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     645     852     901    1154    1520    2049 
   Size:        645     207      49     253     366     529

  Fragments arranged by size: 

                 645     529     366     253     207      49
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1107    2049 
   Size:       1107     942
 
BsrDI GCAATG_nn'

Cuts at:      0      72    1778    1808    2049 
   Size:         72    1706      30     241

  Fragments arranged by size: 

                1706     241      72      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1035    1351    1557    1974    2049 
   Size:       1035     316     206     417      75

  Fragments arranged by size: 

                1035     417     316     206      75
 
BstYI r'GATC_y

Cuts at:      0     131     326    1052    1340    2049 
   Size:        131     195     726     288     709

  Fragments arranged by size: 

                 726     709     288     195     131
 
BtgI C'CryG_G

Cuts at:      0      60     309     755     869    1181    1196    2049 
   Size:         60     249     446     114     312      15     853

  Fragments arranged by size: 

                 853     446     312     249     114      60      15
 
BtsI GCAGTG_nn'

Cuts at:      0    1792    2049 
   Size:       1792     257
 
DraI TTT'AAA

Cuts at:      0    1392    2049 
   Size:       1392     657
 
DraIII CAC_nnn'GTG

Cuts at:      0      97    2049 
   Size:         97    1952
 
EaeI y'GGCC_r

Cuts at:      0     306     312     359     545    1178    2049 
   Size:        306       6      47     186     633     871

  Fragments arranged by size: 

                 871     633     306     186      47       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     579     610    2049 
   Size:        579      31    1439

  Fragments arranged by size: 

                1439     579      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     777     783     846     901    1579    2049 
   Size:        189     588       6      63      55     678     470

  Fragments arranged by size: 

                 678     588     470     189      63      55       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     738     986    1150    1151    2040    2049 
   Size:        738     248     164       1     889       9

  Fragments arranged by size: 

                 889     738     248     164       9       1
 
EcoRI G'AATT_C

Cuts at:      0     673    1042    2049 
   Size:        673     369    1007

  Fragments arranged by size: 

                1007     673     369
 
HaeII r_GCGC'y

Cuts at:      0     202     485    1086    1313    1913    2049 
   Size:        202     283     601     227     600     136

  Fragments arranged by size: 

                 601     600     283     227     202     136
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     821     853    1320    1352    1432    1464    2024    2049 
   Size:        821      32     467      32      80      32     560      25

  Fragments arranged by size: 

                 821     560     467      80      32      32      32      25
            
 
KasI G'GCGC_C

Cuts at:      0     198     481    1309    1909    2049 
   Size:        198     283     828     600     140

  Fragments arranged by size: 

                 828     600     283     198     140
 
KpnI G_GTAC'C

Cuts at:      0    1080    2049 
   Size:       1080     969
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98    1037    2049 
   Size:         98     939    1012

  Fragments arranged by size: 

                1012     939      98
 
MscI TGG'CCA

Cuts at:      0     308    2049 
   Size:        308    1741
 
MslI CAynn'nnrTG

Cuts at:      0     167     970    1349    1381    2049 
   Size:        167     803     379      32     668

  Fragments arranged by size: 

                 803     668     379     167      32
 
MspA1I CmG'CkG

Cuts at:      0      62    2049 
   Size:         62    1987
 
NarI GG'CG_CC

Cuts at:      0     199     482    1310    1910    2049 
   Size:        199     283     828     600     139

  Fragments arranged by size: 

                 828     600     283     199     139
 
NcoI C'CATG_G

Cuts at:      0     309     755     869    1196    2049 
   Size:        309     446     114     327     853

  Fragments arranged by size: 

                 853     446     327     309     114
 
NspI r_CATG'y

Cuts at:      0     420     540    1303    1399    2049 
   Size:        420     120     763      96     650

  Fragments arranged by size: 

                 763     650     420     120      96
 
PciI A'CATG_T

Cuts at:      0     416    1395    2049 
   Size:        416     979     654

  Fragments arranged by size: 

                 979     654     416
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     761     781    2049 
   Size:        761      20    1268

  Fragments arranged by size: 

                1268     761      20
 
PfoI T'CCnGG_A

Cuts at:      0    1556    2049 
   Size:       1556     493
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     985    1017    1507    1539    2049 
   Size:        985      32     490      32     510

  Fragments arranged by size: 

                 985     510     490      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     986    2040    2049 
   Size:        986    1054       9

  Fragments arranged by size: 

                1054     986       9
 
PshAI GACnn'nnGTC

Cuts at:      0     419    2049 
   Size:        419    1630
 
PspOMI G'GGCC_C

Cuts at:      0    1150    2049 
   Size:       1150     899
 
PstI C_TGCA'G

Cuts at:      0    1731    2049 
   Size:       1731     318
 
SacII CC_GC'GG

Cuts at:      0      63    2049 
   Size:         63    1986
 
SanDI GG'GwC_CC

Cuts at:      0     986    2049 
   Size:        986    1063
 
SexAI A'CCwGG_T

Cuts at:      0    1072    2049 
   Size:       1072     977
 
SfcI C'TryA_G

Cuts at:      0    1155    1727    2049 
   Size:       1155     572     322

  Fragments arranged by size: 

                1155     572     322
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     187     358    2049 
   Size:        187     171    1691

  Fragments arranged by size: 

                1691     187     171
 
SfoI GGC'GCC

Cuts at:      0     200     483    1311    1911    2049 
   Size:        200     283     828     600     138

  Fragments arranged by size: 

                 828     600     283     200     138
 
SmaI CCC'GGG

Cuts at:      0    1653    2049 
   Size:       1653     396
 
SmlI C'TyrA_G

Cuts at:      0     949    1696    2030    2049 
   Size:        949     747     334      19

  Fragments arranged by size: 

                 949     747     334      19
 
StuI AGG'CCT

Cuts at:      0     500    2049 
   Size:        500    1549
 
StyI C'CwwG_G

Cuts at:      0     309     755     869     901    1196    2049 
   Size:        309     446     114      32     295     853

  Fragments arranged by size: 

                 853     446     309     295     114      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     179    2049 
   Size:        179    1870
 
TatI w'GTAC_w

Cuts at:      0    1136    1385    1398    2049 
   Size:       1136     249      13     651

  Fragments arranged by size: 

                1136     651     249      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     782    2049 
   Size:        782    1267
 
XhoI C'TCGA_G

Cuts at:      0    2030    2049 
   Size:       2030      19
 
XmaI C'CCGG_G

Cuts at:      0    1651    2049 
   Size:       1651     398
 
XmnI GAAnn'nnTTC

Cuts at:      0    1000    2049 
   Size:       1000    1049
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AlfI    AlwNI     ApaI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI     BglI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BstXI    BstYI     BtgI
     BtsI     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NspI     PciI    PflMI     PfoI     PpiI    PpuMI
    PshAI   PspOMI     PstI    SacII    SanDI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     StuI     StyI    TaqII     TatI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AhdI     AleI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BcgI    BfrBI     BlpI
    BmgBI     BmtI    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     ZraI
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