Restriction map for pF1KSDA0690
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AvrII       BbeI        
BbrPI       BcgI        BciVI       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiWI       Bsp1407I    
BspDI       BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        Eam1105I    EcoRV       EcoT22I     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SbfI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        277  GGTACC               G^GTACC
AccI         3584  GTCGAC               GT^[AC][GT]AC
AcyI          228  GACGCC               G[GA]^CG[CT]C
AflII         871  CTTAAG               C^TTAAG
AflIII       2640  ACATGT               A^C[GA][CT]GT
Alw44I       2292  GTGCAC               G^TGCAC
ApaLI        2292  GTGCAC               G^TGCAC
Asp700I      2715  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       277  GGTACC               G^GTACC
AviII        1694  TGCGCA               TGC^GCA
BfrI          871  CTTAAG               C^TTAAG
BsaHI         228  GACGCC               G[GA]^CG[CT]C
BsaI         1421  GAGACC               GGTCTC(1/5)
BspHI         698  TCATGA               T^CATGA
BspLU11I     2640  ACATGT               A^CATGT
BssSI        2341  CTCGTG               CACGAG(-5/-1)
BstAPI       1344  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         2491  TTTAAA               TTT^AAA
EcoRI         502  GAATTC               G^AATTC
FseI         3436  GGCCGGCC             GGCCGG^CC
FspI         1694  TGCGCA               TGC^GCA
Hin1I         228  GACGCC               G[GA]^CG[CT]C
KpnI          277  GGTACC               GGTAC^C
NheI          861  GCTAGC               G^CTAGC
PshAI        2442  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          698  TCATGA               T^CATGA
SalI         3584  GTCGAC               G^TCGAC
ScaI          437  AGTACT               AGT^ACT
XcmI          759  CCACGCCAGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2715  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccII        1439  CGCG                 CG^CG
             2436  CGCG                
AccIII        455  TCCGGA               T^CCGGA
             2804  TCCGGA              
AcsI          502  GAATTC               [GA]^AATT[CT]
             1144  AAATTC              
Aor51HI        47  AGCGCT               AGC^GCT
              332  AGCGCT              
ApaI         2109  GGGCCC               GGGCC^C
             2117  GGGCCC              
ApoI          502  GAATTC               [GA]^AATT[CT]
             1144  AAATTC              
AspI         1963  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2569  GACGTGGTC           
BamHI        3063  GGATCC               G^GATCC
             3444  GGATCC              
BbsI         2154  GAAGAC               GAAGAC(2/6)
             1186  GTCTTC              
BbvCI        2812  GCTGAGG              CCTCAGC(-5/-2)
             3301  GCTGAGG             
BclI         3161  TGATCA               T^GATCA
             3254  TGATCA              
BglII         368  AGATCT               A^GATCT
             1538  AGATCT              
BpuAI        2154  GAAGAC               GAAGAC(2/6)
             1186  GTCTTC              
BsaWI         455  TCCGGA               [AT]^CCGG[AT]
             2804  TCCGGA              
BseAI         455  TCCGGA               T^CCGGA
             2804  TCCGGA              
BsiEI         130  CGGCCG               CG[GA][CT]^CG
             3439  CGGCCG              
BsmBI         226  GAGACG               CGTCTC(1/5)
              424  GAGACG              
BsmI         1033  GAATGC               GAATGC(1/-1)
             3353  GCATTC              
BspEI         455  TCCGGA               T^CCGGA
             2804  TCCGGA              
BstUI        1439  CGCG                 CG^CG
             2436  CGCG                
DrdI          148  GACCTGACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1666  GACCTGAGGGTC        
EagI          130  CGGCCG               C^GGCCG
             3439  CGGCCG              
EclXI         130  CGGCCG               C^GGCCG
             3439  CGGCCG              
Eco47III       47  AGCGCT               AGC^GCT
              332  AGCGCT              
Eco52I        130  CGGCCG               C^GGCCG
             3439  CGGCCG              
FbaI         3161  TGATCA               T^GATCA
             3254  TGATCA              
HincII       2797  GTCAAC               GT[CT]^[GA]AC
             3584  GTCGAC              
HindII       2797  GTCAAC               GT[CT]^[GA]AC
             3584  GTCGAC              
HindIII      2671  AAGCTT               A^AGCTT
             2707  AAGCTT              
MroI          455  TCCGGA               T^CCGGA
             2804  TCCGGA              
MseI          872  TTAA                 T^TAA
             2492  TTAA                
MvnI         1439  CGCG                 CG^CG
             2436  CGCG                
PflFI        1963  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2569  GACGTGGTC           
PflMI         711  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1296  CCAGGCAGTGG         
SacI          793  GAGCTC               GAGCT^C
             1161  GAGCTC              
SapI          669  GAAGAGC              GCTCTTC(1/4)
             1488  GAAGAGC             
SexAI         899  ACCTGGT              A^CC[AT]GGT
             1964  ACCTGGT             
SmaI         2113  CCCGGG               CCC^GGG
             2819  CCCGGG              
TfiI          402  GATTC                G^A[AT]TC
             1531  GAATC               
Tru9I         872  TTAA                 T^TAA
             2492  TTAA                
Tth111I      1963  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2569  GACGTGGTC           
Van91I        711  CCATGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1296  CCAGGCAGTGG         
XmaCI        2113  CCCGGG               C^CCGGG
             2819  CCCGGG              
XmaI         2113  CCCGGG               C^CCGGG
             2819  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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