Restriction map for pF1KSDA0690
 (Linear) (Six-base) MAPSORT of: KIAA0690.seq  Check: 252  from: 1  to: 3591



KIAA0690.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AccI GT'mk_AC

Cuts at:      0    3585    3591 
   Size:       3585       6
 
Acc65I G'GTAC_C

Cuts at:      0     277    3591 
   Size:        277    3314
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     303     652    1048    1507    1760    2170    3591 
   Size:        108     195     349     396     459     253     410    1421

  Fragments arranged by size: 

                1421     459     410     396     349     253     195     108
            
 
AfeI AGC'GCT

Cuts at:      0      49     334    3591 
   Size:         49     285    3257

  Fragments arranged by size: 

                3257     285      49
 
AflII C'TTAA_G

Cuts at:      0     871    3591 
   Size:        871    2720
 
AflIII A'CryG_T

Cuts at:      0    2640    3591 
   Size:       2640     951
 
AleI CACnn'nnGTG

Cuts at:      0     397    1554    3591 
   Size:        397    1157    2037

  Fragments arranged by size: 

                2037    1157     397
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1090    1179    1245    1692    1729    2529    2611    3591 
   Size:       1090      89      66     447      37     800      82     980

  Fragments arranged by size: 

                1090     980     800     447      89      82      66      37
            
 
ApaI G_GGCC'C

Cuts at:      0    2113    2121    3591 
   Size:       2113       8    1470

  Fragments arranged by size: 

                2113    1470       8
 
ApaLI G'TGCA_C

Cuts at:      0    2292    3591 
   Size:       2292    1299
 
ApoI r'AATT_y

Cuts at:      0     502    1144    3591 
   Size:        502     642    2447

  Fragments arranged by size: 

                2447     642     502
 
AvaI C'yCGr_G

Cuts at:      0       9     273    2113    2773    2819    3591 
   Size:          9     264    1840     660      46     772

  Fragments arranged by size: 

                1840     772     660     264      46       9
 
BamHI G'GATC_C

Cuts at:      0    3063    3444    3591 
   Size:       3063     381     147

  Fragments arranged by size: 

                3063     381     147
 
BanI G'GyrC_C

Cuts at:      0     277    2831    3208    3591 
   Size:        277    2554     377     383

  Fragments arranged by size: 

                2554     383     377     277
 
BanII G_rGCy'C

Cuts at:      0     797    1165    2113    2121    2841    2894    3050    3591 
   Size:        797     368     948       8     720      53     156     541

  Fragments arranged by size: 

                 948     797     720     541     368     156      53       8
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1179    2161    3591 
   Size:       1179     982    1430

  Fragments arranged by size: 

                1430    1179     982
 
BbvCI CC'TCA_GC

Cuts at:      0    2813    3302    3591 
   Size:       2813     489     289

  Fragments arranged by size: 

                2813     489     289
 
BclI T'GATC_A

Cuts at:      0    3161    3254    3591 
   Size:       3161      93     337

  Fragments arranged by size: 

                3161     337      93
 
BglI GCCn_nnn'nGGC

Cuts at:      0     739    2432    3376    3591 
   Size:        739    1693     944     215

  Fragments arranged by size: 

                1693     944     739     215
 
BglII A'GATC_T

Cuts at:      0     368    1538    3591 
   Size:        368    1170    2053

  Fragments arranged by size: 

                2053    1170     368
 
Bme1580I G_kGCm'C

Cuts at:      0    2113    2121    2296    3591 
   Size:       2113       8     175    1295

  Fragments arranged by size: 

                2113    1295     175       8
 
BmgBI CAC'GTC

Cuts at:      0    2571    2622    3591 
   Size:       2571      51     969

  Fragments arranged by size: 

                2571     969      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     830    2596    3591 
   Size:        830    1766     995

  Fragments arranged by size: 

                1766     995     830
 
BmtI G_CTAG'C

Cuts at:      0     865    3591 
   Size:        865    2726
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     288     366    3591 
   Size:        288      78    3225

  Fragments arranged by size: 

                3225     288      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1179    1222    1537    1802    2104    2161    2551 
   Size:        605     574      43     315     265     302      57     390

Cuts at:   2551    3337    3362    3591 
   Size:        786      25     229

  Fragments arranged by size: 

                 786     605     574     390     315     302     265     229
                  57      43      25
 
Bpu10I CC'TnA_GC

Cuts at:      0     775    1511    2813    2842    3302    3591 
   Size:        775     736    1302      29     460     289

  Fragments arranged by size: 

                1302     775     736     460     289      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     726    1000    3268    3591 
   Size:        475     251     274    2268     323

  Fragments arranged by size: 

                2268     475     323     274     251
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1415    3591 
   Size:       1415    2176
 
BsaHI Gr'CG_yC

Cuts at:      0     229    3591 
   Size:        229    3362
 
BsaWI w'CCGG_w

Cuts at:      0     455    2804    3591 
   Size:        455    2349     787

  Fragments arranged by size: 

                2349     787     455
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     421     451    1048    1078    2077    2107    2524    2554 
   Size:        421      30     597      30     999      30     417      30

Cuts at:   2554    3591 
   Size:       1037

  Fragments arranged by size: 

                1037     999     597     421     417      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     679    1065    1141    1390    2101    2175    2791    2875 
   Size:        679     386      76     249     711      74     616      84

Cuts at:   2875    2887    2890    2893    2896    2899    2902    2935    2962 
   Size:         12       3       3       3       3       3      33      27

Cuts at:   2962    2974    2977    3024    3187    3319    3322    3325    3591 
   Size:         12       3      47     163     132       3       3     266

  Fragments arranged by size: 

                 711     679     616     386     266     249     163     132
                  84      76      74      47      33      27      12      12
                   3       3       3       3       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     467    1638    3591 
   Size:        467    1171    1953

  Fragments arranged by size: 

                1953    1171     467
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     598    1572    2080    2149    2567    2665    3591 
   Size:        288     310     974     508      69     418      98     926

  Fragments arranged by size: 

                 974     926     508     418     310     288      98      69
            
 
BsiEI CG_ry'CG

Cuts at:      0     133    3442    3591 
   Size:        133    3309     149

  Fragments arranged by size: 

                3309     149     133
 
BsiHKAI G_wGCw'C

Cuts at:      0     781     797    1165    1922    2182    2296    3591 
   Size:        781      16     368     757     260     114    1295

  Fragments arranged by size: 

                1295     781     757     368     260     114      16
 
BsmI GAATG_Cn'

Cuts at:      0    1039    3353    3591 
   Size:       1039    2314     238

  Fragments arranged by size: 

                2314    1039     238
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     220     418    3591 
   Size:        220     198    3173

  Fragments arranged by size: 

                3173     220     198
 
Bsp1286I G_dGCh'C

Cuts at:      0     781     797    1165    1922    2113    2121    2182    2296 
   Size:        781      16     368     757     191       8      61     114

Cuts at:   2296    2841    2894    3050    3591 
   Size:        545      53     156     541

  Fragments arranged by size: 

                 781     757     545     541     368     191     156     114
                  61      53      16       8
 
BspEI T'CCGG_A

Cuts at:      0     455    2804    3591 
   Size:        455    2349     787

  Fragments arranged by size: 

                2349     787     455
 
BspHI T'CATG_A

Cuts at:      0     698    3591 
   Size:        698    2893
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     647     973    1262    3591 
   Size:        647     326     289    2329

  Fragments arranged by size: 

                2329     647     326     289
 
BsrFI r'CCGG_y

Cuts at:      0     128    3155    3437    3591 
   Size:        128    3027     282     154

  Fragments arranged by size: 

                3027     282     154     128
 
BssSI C'ACGA_G

Cuts at:      0    2341    3591 
   Size:       2341    1250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1350    3591 
   Size:       1350    2241
 
BstEII G'GTnAC_C

Cuts at:      0     960    1314    1674    2439    3591 
   Size:        960     354     360     765    1152

  Fragments arranged by size: 

                1152     960     765     360     354
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1400    1506    2517    3591 
   Size:       1400     106    1011    1074

  Fragments arranged by size: 

                1400    1074    1011     106
 
BstYI r'GATC_y

Cuts at:      0     368    1022    1538    3063    3444    3591 
   Size:        368     654     516    1525     381     147

  Fragments arranged by size: 

                1525     654     516     381     368     147
 
Bsu36I CC'TnA_GG

Cuts at:      0     691    1083    1331    1669    2105    2365    3591 
   Size:        691     392     248     338     436     260    1226

  Fragments arranged by size: 

                1226     691     436     392     338     260     248
 
BtgI C'CryG_G

Cuts at:      0     233    1393    1499    1799    2052    2444    2855    3591 
   Size:        233    1160     106     300     253     392     411     736

  Fragments arranged by size: 

                1160     736     411     392     300     253     233     106
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3343    3591 
   Size:       3343     248
 
BtsI GCAGTG_nn'

Cuts at:      0     504     548     684     919    1127    1307    2158    2264 
   Size:        504      44     136     235     208     180     851     106

Cuts at:   2264    2335    2404    2612    3591 
   Size:         71      69     208     979

  Fragments arranged by size: 

                 979     851     504     235     208     208     180     136
                 106      71      69      44
 
DraI TTT'AAA

Cuts at:      0    2493    3591 
   Size:       2493    1098
 
DraIII CAC_nnn'GTG

Cuts at:      0    1221    1682    2058    2229    3591 
   Size:       1221     461     376     171    1362

  Fragments arranged by size: 

                1362    1221     461     376     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     154    1672    3591 
   Size:        154    1518    1919

  Fragments arranged by size: 

                1919    1518     154
 
EaeI y'GGCC_r

Cuts at:      0     130    1607    1646    2633    3089    3153    3368    3439 
   Size:        130    1477      39     987     456      64     215      71

Cuts at:   3439    3591 
   Size:        152

  Fragments arranged by size: 

                1477     987     456     215     152     130      71      64
                  39
 
EagI C'GGCC_G

Cuts at:      0     130    3439    3591 
   Size:        130    3309     152

  Fragments arranged by size: 

                3309     152     130
 
EarI CTCTTCn'nnn_

Cuts at:      0     236     664     760    1483    1783    2128    2381    2864 
   Size:        236     428      96     723     300     345     253     483

Cuts at:   2864    3194    3221    3591 
   Size:        330      27     370

  Fragments arranged by size: 

                 723     483     428     370     345     330     300     253
                 236      96      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     262    3591 
   Size:        262    3329
 
EcoICRI GAG'CTC

Cuts at:      0     795    1163    3591 
   Size:        795     368    2428

  Fragments arranged by size: 

                2428     795     368
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     108     303     605     652    1048    1179    1222    1507 
   Size:        108     195     302      47     396     131      43     285

Cuts at:   1507    1537    1760    1802    2104    2161    2170    2551    3337 
   Size:         30     223      42     302      57       9     381     786

Cuts at:   3337    3362    3591 
   Size:         25     229

  Fragments arranged by size: 

                 786     396     381     302     302     285     229     223
                 195     131     108      57      47      43      42      30
                  25       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1510    2845    3529    3591 
   Size:       1510    1335     684      62

  Fragments arranged by size: 

                1510    1335     684      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     519     636     918    1087    1131    1327    1689 
   Size:        194     325     117     282     169      44     196     362

Cuts at:   1689    2109    2110    2117    2118    2145    2201    2391    3591 
   Size:        420       1       7       1      27      56     190    1200

  Fragments arranged by size: 

                1200     420     362     325     282     196     194     190
                 169     117      56      44      27       7       1       1
            
 
EcoRI G'AATT_C

Cuts at:      0     502    3591 
   Size:        502    3089
 
FseI GG_CCGG'CC

Cuts at:      0    3441    3591 
   Size:       3441     150
 
FspI TGC'GCA

Cuts at:      0    1696    3591 
   Size:       1696    1895
 
HaeII r_GCGC'y

Cuts at:      0      51     336    3149    3591 
   Size:         51     285    2813     442

  Fragments arranged by size: 

                2813     442     285      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204    1048    1080    1940    1972    2780    2812 
   Size:        172      32     844      32     860      32     808      32

Cuts at:   2812    3312    3344    3591 
   Size:        500      32     247

  Fragments arranged by size: 

                 860     844     808     500     247     172      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2799    3586    3591 
   Size:       2799     787       5

  Fragments arranged by size: 

                2799     787       5
 
HindIII A'AGCT_T

Cuts at:      0    2671    2707    3591 
   Size:       2671      36     884

  Fragments arranged by size: 

                2671     884      36
 
KpnI G_GTAC'C

Cuts at:      0     281    3591 
   Size:        281    3310
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     371    3591 
   Size:        323      48    3220

  Fragments arranged by size: 

                3220     323      48
 
MscI TGG'CCA

Cuts at:      0    1609    1648    2635    3091    3370    3591 
   Size:       1609      39     987     456     279     221

  Fragments arranged by size: 

                1609     987     456     279     221      39
 
MslI CAynn'nnrTG

Cuts at:      0     397    1554    2599    2620    3591 
   Size:        397    1157    1045      21     971

  Fragments arranged by size: 

                1157    1045     971     397      21
 
MspA1I CmG'CkG

Cuts at:      0    1176    2245    2453    2526    2649    2991    3591 
   Size:       1176    1069     208      73     123     342     600

  Fragments arranged by size: 

                1176    1069     600     342     208     123      73
 
NaeI GCC'GGC

Cuts at:      0     130    3157    3439    3591 
   Size:        130    3027     282     152

  Fragments arranged by size: 

                3027     282     152     130
 
NcoI C'CATG_G

Cuts at:      0     233    1499    2444    3591 
   Size:        233    1266     945    1147

  Fragments arranged by size: 

                1266    1147     945     233
 
NgoMIV G'CCGG_C

Cuts at:      0     128    3155    3437    3591 
   Size:        128    3027     282     154

  Fragments arranged by size: 

                3027     282     154     128
 
NheI G'CTAG_C

Cuts at:      0     861    3591 
   Size:        861    2730
 
NspI r_CATG'y

Cuts at:      0     621    2644    2688    3591 
   Size:        621    2023      44     903

  Fragments arranged by size: 

                2023     903     621      44
 
PasI CC'CwG_GG

Cuts at:      0     411    2898    3591 
   Size:        411    2487     693

  Fragments arranged by size: 

                2487     693     411
 
PciI A'CATG_T

Cuts at:      0    2640    3591 
   Size:       2640     951
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     717    1302    3591 
   Size:        717     585    2289

  Fragments arranged by size: 

                2289     717     585
 
PfoI T'CCnGG_A

Cuts at:      0     566     653    1895    1958    3059    3591 
   Size:        566      87    1242      63    1101     532

  Fragments arranged by size: 

                1242    1101     566     532      87      63
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1412    1444    3591 
   Size:       1412      32    2147

  Fragments arranged by size: 

                2147    1412      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     194     636     918    1327    2145    3591 
   Size:        194     442     282     409     818    1446

  Fragments arranged by size: 

                1446     818     442     409     282     194
 
PshAI GACnn'nnGTC

Cuts at:      0    2446    3591 
   Size:       2446    1145
 
PspOMI G'GGCC_C

Cuts at:      0    2109    2117    3591 
   Size:       2109       8    1474

  Fragments arranged by size: 

                2109    1474       8
 
PstI C_TGCA'G

Cuts at:      0     188     679    1346    2399    2458    3509    3591 
   Size:        188     491     667    1053      59    1051      82

  Fragments arranged by size: 

                1053    1051     667     491     188      82      59
 
PvuII CAG'CTG

Cuts at:      0    1176    2245    2453    2526    2649    3591 
   Size:       1176    1069     208      73     123     942

  Fragments arranged by size: 

                1176    1069     942     208     123      73
 
SacI G_AGCT'C

Cuts at:      0     797    1165    3591 
   Size:        797     368    2426

  Fragments arranged by size: 

                2426     797     368
 
SalI G'TCGA_C

Cuts at:      0    3584    3591 
   Size:       3584       7
 
SapI GCTCTTCn'nnn_

Cuts at:      0     664    1483    3591 
   Size:        664     819    2108

  Fragments arranged by size: 

                2108     819     664
 
ScaI AGT'ACT

Cuts at:      0     439    3591 
   Size:        439    3152
 
SexAI A'CCwGG_T

Cuts at:      0     899    1964    3591 
   Size:        899    1065    1627

  Fragments arranged by size: 

                1627    1065     899
 
SfcI C'TryA_G

Cuts at:      0     184     675    1342    1598    2395    2454    3505    3591 
   Size:        184     491     667     256     797      59    1051      86

  Fragments arranged by size: 

                1051     797     667     491     256     184      86      59
            
 
SmaI CCC'GGG

Cuts at:      0    2115    2821    3591 
   Size:       2115     706     770

  Fragments arranged by size: 

                2115     770     706
 
SmlI C'TyrA_G

Cuts at:      0     490     705     871    1015    3283    3591 
   Size:        490     215     166     144    2268     308

  Fragments arranged by size: 

                2268     490     308     215     166     144
 
SrfI GCCC'GGGC

Cuts at:      0    2821    3591 
   Size:       2821     770
 
StuI AGG'CCT

Cuts at:      0     662     742     768    1596    2071    3524    3591 
   Size:        662      80      26     828     475    1453      67

  Fragments arranged by size: 

                1453     828     662     475      80      67      26
 
StyI C'CwwG_G

Cuts at:      0     233     593    1499    1709    1976    2297    2444    3068 
   Size:        233     360     906     210     267     321     147     624

Cuts at:   3068    3591 
   Size:        523

  Fragments arranged by size: 

                 906     624     523     360     321     267     233     210
                 147
 
TatI w'GTAC_w

Cuts at:      0     327     437    2512    3591 
   Size:        327     110    2075    1079

  Fragments arranged by size: 

                2075    1079     327     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     944     976    1319    1351    2077    2109    2524    2556 
   Size:        944      32     343      32     726      32     415      32

Cuts at:   2556    3591 
   Size:       1035

  Fragments arranged by size: 

                1035     944     726     415     343      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1966    2572    3591 
   Size:       1966     606    1019

  Fragments arranged by size: 

                1966    1019     606
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     766    3591 
   Size:        766    2825
 
XmaI C'CCGG_G

Cuts at:      0    2113    2819    3591 
   Size:       2113     706     772

  Fragments arranged by size: 

                2113     772     706
 
XmnI GAAnn'nnTTC

Cuts at:      0    2719    3591 
   Size:       2719     872
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrFI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI     SalI     SapI     ScaI    SexAI
     SfcI     SmaI     SmlI     SrfI     StuI     StyI     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AgeI     AhdI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI
     BlpI    BsaAI    BsaBI    BsiWI    BsrBI    BsrDI    BsrGI   BssHII
    BstBI  BstZ17I     ClaI    CspCI    EcoRV     FalI    FspAI     HpaI
     KasI     MfeI     MluI     NarI     NdeI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     SwaI    TaqII     XbaI     XhoI     ZraI
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