Restriction map for pF1KSDA0680
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BcnI        
BglI        BglII       BlnI        BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        ClaI        CpoI        
DraIII      EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT22I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      KasI        
KpnI        KspI        MaeII       MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI         1874  GTCGAC               GT^[AC][GT]AC
AciI         1767  CCGC                 CCGC(-3/-1)
BamHI        1715  GGATCC               G^GATCC
BcgI          700  GCATCAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1428  GGATAC               GTATCC(6/5)
BclI          255  TGATCA               T^GATCA
BmyI          298  GGGCAC               G[AGT]GC[ACT]^C
BsaBI         306  GATACCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1092  CGTCTC               CGTCTC(1/5)
Bsp1286I      298  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I     1349  TGTACA               T^GTACA
BspHI        1863  TCATGA               T^CATGA
BsrFI         447  ACCGGC               [GA]^CCGG[CT]
BsrGI        1349  TGTACA               T^GTACA
BstAPI        451  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        447  ACCGGC               [GA]^CCGG[CT]
DraI         1840  TTTAAA               TTT^AAA
DraII         608  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1084  GACCTGCCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          965  CCATGG               C^C[GA][CT]GG
EcoO109I      608  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         195  GATATC               GAT^ATC
FbaI          255  TGATCA               T^GATCA
MamI          306  GATACCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          965  CCATGG               C^CATGG
PshAI        1105  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1863  TCATGA               T^CATGA
SalI         1874  GTCGAC               G^TCGAC
SapI         1129  GAAGAGC              GCTCTTC(1/4)
SfaNI         700  GCATC                GCATC(5/9)
SspBI        1349  TGTACA               T^GTACA
Tsp45I       1744  GTCAC                ^GT[GC]AC
XcmI         1208  CCAAAGAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI         1350  GTAC                 GT^AC
             1499  GTAC                
AflII         241  CTTAAG               C^TTAAG
             1170  CTTAAG              
AvaI          329  CCCGAG               C^[CT]CG[GA]G
              650  CCCGAG              
AvaII         785  GGACC                G^G[AT]CC
              969  GGTCC               
BbvCI        1234  CCTCAGC              CCTCAGC(-5/-2)
              934  GCTGAGG             
BfrI          241  CTTAAG               C^TTAAG
             1170  CTTAAG              
BlpI         1560  GCTGAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
Bpu1102I     1560  GCTGAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
BsaI          889  GAGACC               GGTCTC(1/5)
             1683  GAGACC              
BsoBI         329  CCCGAG               C^[CT]CG[GA]G
              650  CCCGAG              
BstYI         490  AGATCC               [GA]^GATC[CT]
             1715  GGATCC              
CelII        1560  GCTGAGC              GC^T[ACGT]AGC
             1674  GCTCAGC             
EcoNI         523  CCTCCGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1784  CCTGGGCCAGG         
HapII         448  CCGG                 C^CGG
             1422  CCGG                
HindIII        29  AAGCTT               A^AGCTT
              743  AAGCTT              
HpaII         448  CCGG                 C^CGG
             1422  CCGG                
MaeIII        271  GTAAC                ^GT[ACGT]AC
             1744  GTCAC               
MflI          490  AGATCC               [GA]^GATC[CT]
             1715  GGATCC              
MslI          553  CACAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1848  CACAGAAATG          
MspI          448  CCGG                 C^CGG
             1422  CCGG                
PstI          884  CTGCAG               CTGCA^G
             1802  CTGCAG              
RsaI         1350  GTAC                 GT^AC
             1499  GTAC                
XhoII         490  AGATCC               [GA]^GATC[CT]
             1715  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AcsI        AluI        AlwI        AlwNI       ApoI        
BbsI        BbvI        BfaI        BpmI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HpaI        
HphI        ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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