Restriction map for pF1KSDA0680
 (Circular) (Six-base) MAPSORT of: pF1KSDA0680.seq  Check: 7083  from: 1  to: 5039



pF1KSDA0680  5039 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1935    1935 
   Size:       5039
 
AccI GT'mk_AC

Cuts at:   2001    2077    2001 
   Size:         76    4963
 
Acc65I G'GTAC_C

Cuts at:   2055    2055 
   Size:       5039
 
AclI AA'CG_TT

Cuts at:   4879    4879 
   Size:       5039
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    500    1274    1379    1652    1727    2864    3296    4043    4311 
   Size:        774     105     273      75    1137     432     747     268

Cuts at:   4311     500 
   Size:       1228

  Fragments arranged by size: 

                1228    1137     774     747     432     273     268     105
                  75
 
AflII C'TTAA_G

Cuts at:    367    1296     367 
   Size:        929    4110
 
AflIII A'CryG_T

Cuts at:   2391    3495    4472    2391 
   Size:       1104     977    2958

  Fragments arranged by size: 

                2958    1104     977
 
AgeI A'CCGG_T

Cuts at:   3427    3427 
   Size:       5039
 
AleI CACnn'nnGTG

Cuts at:    683     683 
   Size:       5039
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1034    1066    1034 
   Size:         32    5007
 
AlwNI CAG_nnn'CTG

Cuts at:    583    1291    1368    2344    3911     583 
   Size:        708      77     976    1567    1711

  Fragments arranged by size: 

                1711    1567     976     708      77
 
ApaLI G'TGCA_C

Cuts at:   3809    3809 
   Size:       5039
 
ApoI r'AATT_y

Cuts at:    286     414    1081    1963    2043    4392    4648     286 
   Size:        128     667     882      80    2349     256     677

  Fragments arranged by size: 

                2349     882     677     667     256     128      80
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5039
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5039
 
AvaI C'yCGr_G

Cuts at:    455     776    2059     455 
   Size:        321    1283    3435

  Fragments arranged by size: 

                3435    1283     321
 
BamHI G'GATC_C

Cuts at:   1841    2064    1841 
   Size:        223    4816
 
BanI G'GyrC_C

Cuts at:   2055    2469    2717    2752    2055 
   Size:        414     248      35    4342

  Fragments arranged by size: 

                4342     414     248      35
 
BanII G_rGCy'C

Cuts at:   2053    3083    2053 
   Size:       1030    4009
 
BbeI G_GCGC'C

Cuts at:   2473    2721    2473 
   Size:        248    4791
 
BbsI GAAGACnn'nnnn_

Cuts at:    280    1357    1502     280 
   Size:       1077     145    3817

  Fragments arranged by size: 

                3817    1077     145
 
BbvCI CC'TCA_GC

Cuts at:   1061    1361    1061 
   Size:        300    4739
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    815     849     815 
   Size:         34    5005
 
BciVI GTATCCnnnnn_n'

Cuts at:   1548    2937    3698    5039    1548 
   Size:       1389     761    1341    1548

  Fragments arranged by size: 

                1548    1389    1341     761
 
BclI T'GATC_A

Cuts at:    381    2559     381 
   Size:       2178    2861
 
BglII A'GATC_T

Cuts at:   2554    4591    2554 
   Size:       2037    3002
 
BlpI GC'TnA_GC

Cuts at:   1687    1801    2151    1687 
   Size:        114     350    4575

  Fragments arranged by size: 

                4575     350     114
 
Bme1580I G_kGCm'C

Cuts at:    428    2664    2757    3813     428 
   Size:       2236      93    1056    1654

  Fragments arranged by size: 

                2236    1654    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2251    2332    2667    4811    2251 
   Size:         81     335    2144    2479

  Fragments arranged by size: 

                2479    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1574    3406    1574 
   Size:       1832    3207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    541    1076    1100    1166     541 
   Size:        535      24      66    4414

  Fragments arranged by size: 

                4414     535      66      24
 
Bpu10I CC'TnA_GC

Cuts at:    608    1061    1361     608 
   Size:        453     300    4286

  Fragments arranged by size: 

                4286     453     300
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1171    2215    3094    3586    3884    1171 
   Size:       1044     879     492     298    2326

  Fragments arranged by size: 

                2326    1044     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1009    1803    4743    1009 
   Size:        794    2940    1305

  Fragments arranged by size: 

                2940    1305     794
 
BsaAI yAC'GTr

Cuts at:   2031    2392    2031 
   Size:        361    4678
 
BsaBI GATnn'nnATC

Cuts at:      6     436    2553    4590       6 
   Size:        430    2117    2037     455

  Fragments arranged by size: 

                2117    2037     455     430
 
BsaHI Gr'CG_yC

Cuts at:   2470    2718    3420    4916    2470 
   Size:        248     702    1496    2593

  Fragments arranged by size: 

                2593    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2228    2749    3427    3701    3848    4259    2228 
   Size:        521     678     274     147     411    3008

  Fragments arranged by size: 

                3008     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    705     735    1863    1893     705 
   Size:         30    1128      30    3851

  Fragments arranged by size: 

                3851    1128      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    358     593     783    1078    1118    1354     358 
   Size:        235     190     295      40     236    4043

  Fragments arranged by size: 

                4043     295     236     235     190      40
 
BseYI C'CCAG_C

Cuts at:    667    1344    2464    3222    3799    4295     667 
   Size:        677    1120     758     577     496    1411

  Fragments arranged by size: 

                1411    1120     758     677     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2581    2627    3835     104 
   Size:       2477      46    1208    1308

  Fragments arranged by size: 

                2477    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2053    2831    3021    3813    2053 
   Size:        778     190     792    3279

  Fragments arranged by size: 

                3279     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1224    1224 
   Size:       5039
 
Bsp1286I G_dGCh'C

Cuts at:    428    2053    2664    2757    2831    3021    3083    3813     428 
   Size:       1625     611      93      74     190      62     730    1654

  Fragments arranged by size: 

                1654    1625     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   4259    4259 
   Size:       5039
 
BspHI T'CATG_A

Cuts at:   1989    4215    1989 
   Size:       2226    2813
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    178    1220    1935    2089    2303    2605    2986     178 
   Size:       1042     715     154     214     302     381    2231

  Fragments arranged by size: 

                2231    1042     715     381     302     214     154
 
BsrBI CCG'CTC

Cuts at:     50    3331    3385    4869    5034      50 
   Size:       3281      54    1484     165      55

  Fragments arranged by size: 

                3281    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2951    2951 
   Size:       5039
 
BsrFI r'CCGG_y

Cuts at:    573    3037    3218    3427     573 
   Size:       2464     181     209    2185

  Fragments arranged by size: 

                2464    2185     209     181
 
BsrGI T'GTAC_A

Cuts at:   1475    1475 
   Size:       5039
 
BssSI C'ACGA_G

Cuts at:   3310    3668    3310 
   Size:        358    4681
 
BstAPI GCAn_nnn'nTGC

Cuts at:    583    2416     583 
   Size:       1833    3206
 
BstBI TT'CG_AA

Cuts at:    110    3400     110 
   Size:       3290    1749
 
BstYI r'GATC_y

Cuts at:      1     616    1841    2064    2531    2554    2889    3135    4147 
   Size:        615    1225     223     467      23     335     246    1012

Cuts at:   4147    4233    4245    4591       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1225    1012     615     467     449     346     335     246
                 223      86      23      12
 
BtgI C'CryG_G

Cuts at:    125    1091     125 
   Size:        966    4073
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2989    3170    3333    2989 
   Size:        181     163    4695

  Fragments arranged by size: 

                4695     181     163
 
BtsI GCAGTG_nn'

Cuts at:    662    1521    2282     662 
   Size:        859     761    3419

  Fragments arranged by size: 

                3419     859     761
 
DraI TTT'AAA

Cuts at:   1968    2038    1968 
   Size:         70    4969
 
DrdI GACnn_nn'nnGTC

Cuts at:   1216    2745    3603    1216 
   Size:       1529     858    2652

  Fragments arranged by size: 

                2652    1529     858
 
EaeI y'GGCC_r

Cuts at:   2624    2798    3189    3216    4561    2624 
   Size:        174     391      27    1345    3102

  Fragments arranged by size: 

                3102    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2624    2624 
   Size:       5039
 
EarI CTCTTCn'nnn_

Cuts at:   1250    1631    1745    1988    3062    3272    1250 
   Size:        381     114     243    1074     210    3017

  Fragments arranged by size: 

                3017    1074     381     243     210     114
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3247    3557    3703    4849    3247 
   Size:        310     146    1146    3437

  Fragments arranged by size: 

                3437    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2051    2051 
   Size:       5039
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    500     541    1076    1100    1166    1274    1379    1652    1727 
   Size:         41     535      24      66     108     105     273      75

Cuts at:   1727    2864    3296    4043    4311     500 
   Size:       1137     432     747     268    1228

  Fragments arranged by size: 

                1228    1137     747     535     432     273     268     108
                 105      75      66      41      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:    653    1914     653 
   Size:       1261    3778
 
EcoO109I rG'GnC_Cy

Cuts at:    735    2178     735 
   Size:       1443    3596
 
EcoRI G'AATT_C

Cuts at:   2043    2043 
   Size:       5039
 
EcoRV GAT'ATC

Cuts at:    323     323 
   Size:       5039
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3058    3058 
   Size:       5039
 
FspI TGC'GCA

Cuts at:   2820    2820 
   Size:       5039
 
HaeII r_GCGC'y

Cuts at:   2473    2721    3743    4722    2473 
   Size:        248    1022     979    2790

  Fragments arranged by size: 

                2790    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1344    1376    1441    1473    2881    2911    2913    2943    1344 
   Size:         32      65      32    1408      30       2      30    3440

  Fragments arranged by size: 

                3440    1408      65      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    141     405    1305    1922    2002    2078     141 
   Size:        264     900     617      80      76    3102

  Fragments arranged by size: 

                3102     900     617     264      80      76
 
HindIII A'AGCT_T

Cuts at:    155     869     155 
   Size:        714    4325
 
HpaI GTT'AAC

Cuts at:    405    1305    1922     405 
   Size:        900     617    3522

  Fragments arranged by size: 

                3522     900     617
 
KasI G'GCGC_C

Cuts at:   2469    2717    2469 
   Size:        248    4791
 
KpnI G_GTAC'C

Cuts at:   2059    2059 
   Size:       5039
 
MluI A'CGCG_T

Cuts at:   4472    4472 
   Size:       5039
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1016    1182    1677    3710    3894    1016 
   Size:        166     495    2033     184    2161

  Fragments arranged by size: 

                2161    2033     495     184     166
 
MscI TGG'CCA

Cuts at:   2800    2800 
   Size:       5039
 
MslI CAynn'nnrTG

Cuts at:    683    1978    3028    3155     683 
   Size:       1295    1050     127    2567

  Fragments arranged by size: 

                2567    1295    1050     127
 
MspA1I CmG'CkG

Cuts at:   2150    2464    2824    3837    4082    2150 
   Size:        314     360    1013     245    3107

  Fragments arranged by size: 

                3107    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   3220    3220 
   Size:       5039
 
NarI GG'CG_CC

Cuts at:   2470    2718    2470 
   Size:        248    4791
 
NcoI C'CATG_G

Cuts at:    125    1091     125 
   Size:        966    4073
 
NgoMIV G'CCGG_C

Cuts at:   3218    3218 
   Size:       5039
 
NspI r_CATG'y

Cuts at:   2092    2592    3499    2092 
   Size:        500     907    3632

  Fragments arranged by size: 

                3632     907     500
 
PasI CC'CwG_GG

Cuts at:   1910    1910 
   Size:       5039
 
PciI A'CATG_T

Cuts at:   3495    3495 
   Size:       5039
 
PmeI GTTT'AAAC

Cuts at:   2038    2038 
   Size:       5039
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4178    4210    4178 
   Size:         32    5007
 
PshAI GACnn'nnGTC

Cuts at:   1235    1235 
   Size:       5039
 
PstI C_TGCA'G

Cuts at:   1014    1932    2086    2771    1014 
   Size:        918     154     685    3282

  Fragments arranged by size: 

                3282     918     685     154
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5039
 
PvuII CAG'CTG

Cuts at:   2464    2824    2464 
   Size:        360    4679
 
RsrII CG'GwC_CG

Cuts at:   3234    3234 
   Size:       5039
 
SacI G_AGCT'C

Cuts at:   2053    2053 
   Size:       5039
 
SalI G'TCGA_C

Cuts at:   2000    2076    2000 
   Size:         76    4963
 
SapI GCTCTTCn'nnn_

Cuts at:   1250    3062    3272    1250 
   Size:       1812     210    3017

  Fragments arranged by size: 

                3017    1812     210
 
SbfI CC_TGCA'GG

Cuts at:   2086    2086 
   Size:       5039
 
ScaI AGT'ACT

Cuts at:   4556    4556 
   Size:       5039
 
SfcI C'TryA_G

Cuts at:     33     161    1010    1928    2082    2767    3760    3951      33 
   Size:        128     849     918     154     685     993     191    1121

  Fragments arranged by size: 

                1121     993     918     849     685     191     154     128
            
 
SfoI GGC'GCC

Cuts at:   2471    2719    2471 
   Size:        248    4791
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5039
 
SmaI CCC'GGG

Cuts at:   2061    2061 
   Size:       5039
 
SmlI C'TyrA_G

Cuts at:    367    1150    1296    2194    3109    3601    3863     367 
   Size:        783     146     898     915     492     262    1543

  Fragments arranged by size: 

                1543     915     898     783     492     262     146
 
SnaBI TAC'GTA

Cuts at:   2031    2031 
   Size:       5039
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5039
 
SphI G_CATG'C

Cuts at:   2092    2592    2092 
   Size:        500    4539
 
StyI C'CwwG_G

Cuts at:    125     253     716     917    1091    2173    2527     125 
   Size:        128     463     201     174    1082     354    2637

  Fragments arranged by size: 

                2637    1082     463     354     201     174     128
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    765    2622    2755    3214    3423     765 
   Size:       1857     133     459     209    2381

  Fragments arranged by size: 

                2381    1857     459     209     133
 
TatI w'GTAC_w

Cuts at:   1475    4554    1475 
   Size:       3079    1960
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1863    1895    4556    4588    1863 
   Size:         32    2661      32    2314

  Fragments arranged by size: 

                2661    2314      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2836    2836 
   Size:       5039
 
XbaI T'CTAG_A

Cuts at:   2070    2070 
   Size:       5039
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1341    2534    1341 
   Size:       1193    3846
 
XmaI C'CCGG_G

Cuts at:   2059    2059 
   Size:       5039
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI    PshAI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AlfI     ApaI     AscI    AvrII     BaeI
    BfrBI     BglI    BmgBI     BmtI     BsgI    BsiWI     BsmI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII     FseI
    FspAI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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