Restriction map for pF1KSDA0638
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AclI        AcsI        AgeI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
AspI        AvrII       BamHI       BcgI        BciVI       
BclI        BglII       BlnI        BsaBI       BsiWI       
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HpaI        KspI        MamI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2761  GTAGAC               GT^[AC][GT]AC
AcyI          718  GGCGCC               G[GA]^CG[CT]C
AflII        1377  CTTAAG               C^TTAAG
AflIII       3407  ACATGT               A^C[GA][CT]GT
AhdI         3403  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1891  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1280  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3403  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3944  TGCGCA               TGC^GCA
BbeI          718  GGCGCC               GGCGC^C
BfrI         1377  CTTAAG               C^TTAAG
BsaHI         718  GGCGCC               G[GA]^CG[CT]C
BsiEI        3840  CGACCG               CG[GA][CT]^CG
BsmBI        3883  GAGACG               CGTCTC(1/5)
BspHI        4124  TCATGA               T^CATGA
BspLU11I     3407  ACATGT               A^CATGT
BsrDI         299  GCAATG               GCAATG(2/0)
BssSI        1961  CACGAG               CACGAG(-5/-1)
BstXI         722  CCATGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1891  CCTGAGG              CC^T[ACGT]AGG
Eam1105I     3403  GACAACATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1891  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3537  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI         2475  GGCCGGCC             GGCCGG^CC
FspI         3944  TGCGCA               TGC^GCA
Hin1I         718  GGCGCC               G[GA]^CG[CT]C
HincII       1118  GTCAAC               GT[CT]^[GA]AC
HindII       1118  GTCAAC               GT[CT]^[GA]AC
HindIII      2858  AAGCTT               A^AGCTT
KasI          718  GGCGCC               G^GCGCC
NarI          718  GGCGCC               GG^CGCC
NheI         2139  GCTAGC               G^CTAGC
RcaI         4124  TCATGA               T^CATGA
SexAI         122  ACCTGGT              A^CC[AT]GGT
SfiI         1339  GGCCGGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          718  GGCGCC               GGC^GCC
SgrAI        1777  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         2609  CCCGGG               CCC^GGG
Tsp509I      3644  AATT                 ^AATT
XmaCI        2609  CCCGGG               C^CCGGG
XmaI         2609  CCCGGG               C^CCGGG
XmnI         1280  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I        852  GGTACC               G^GTACC
             2777  GGTACC              
AccIII       2492  TCCGGA               T^CCGGA
             3683  TCCGGA              
Asp718I       852  GGTACC               G^GTACC
             2777  GGTACC              
BalI         1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
BbrPI        2176  CACGTG               CAC^GTG
             3751  CACGTG              
BbvCI        3181  GCTGAGG              CCTCAGC(-5/-2)
             4105  GCTGAGG             
BsaAI        2176  CACGTG               [CT]AC^GT[GA]
             3751  CACGTG              
BsaWI        2492  TCCGGA               [AT]^CCGG[AT]
             3683  TCCGGA              
BseAI        2492  TCCGGA               T^CCGGA
             3683  TCCGGA              
BsmI         2619  GAATGC               GAATGC(1/-1)
             1130  GCATTC              
BspEI        2492  TCCGGA               T^CCGGA
             3683  TCCGGA              
BstYI        1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              
HgaI            4  GACGC                GACGC(5/10)
             1521  GACGC               
KpnI          852  GGTACC               GGTAC^C
             2777  GGTACC              
MaeII        2177  ACGT                 A^CGT
             3752  ACGT                
MflI         1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              
MluNI        1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
MroI         2492  TCCGGA               T^CCGGA
             3683  TCCGGA              
MscI         1076  TGGCCA               TGG^CCA
             2543  TGGCCA              
NaeI         1340  GCCGGC               GCC^GGC
             2476  GCCGGC              
NgoMIV       1340  GCCGGC               G^CCGGC
             2476  GCCGGC              
NspI          359  GCATGT               [GA]CATG^[CT]
             3407  ACATGT              
PmaCI        2176  CACGTG               CAC^GTG
             3751  CACGTG              
PmlI         2176  CACGTG               CAC^GTG
             3751  CACGTG              
TfiI          432  GATTC                G^A[AT]TC
              484  GAATC               
XhoII        1434  AGATCC               [GA]^GATC[CT]
             2570  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       Aor51HI     ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
Eco47III    EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        
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