Restriction map for pF1KSDA0604
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AlwI        AocI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BfrI        BsaAI       BsaBI       
BsiWI       BsmBI       BspDI       BspHI       BsrBI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        DraI        DraIII      DrdI        Eam1105I    
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          703  GTCTAC               GT^[AC][GT]AC
AflIII       1583  ACATGT               A^C[GA][CT]GT
Aor51HI       524  AGCGCT               AGC^GCT
ApaI          625  GGGCCC               GGGCC^C
Asp700I       423  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         240  CCTAGG               C^CTAGG
BalI          926  TGGCCA               TGG^CCA
BbeI         1011  GGCGCC               GGCGC^C
BclI         1385  TGATCA               T^GATCA
BcnI          111  CCCGG                CC^[GC]GG
BfaI          241  CTAG                 C^TAG
BglII         971  AGATCT               A^GATCT
BlnI          240  CCTAGG               C^CTAGG
BlpI          508  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      508  GCTGAGC              GC^T[ACGT]AGC
BsiEI        1965  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1595  TGTACA               T^GTACA
BspLU11I     1583  ACATGT               A^CATGT
BsrFI        1355  ACCGGC               [GA]^CCGG[CT]
BsrGI        1595  TGTACA               T^GTACA
BstEII       2166  GGTGACC              G^GT[ACGT]ACC
BstPI        2166  GGTGACC              G^GT[ACGT]ACC
BstYI         971  AGATCT               [GA]^GATC[CT]
CelII         508  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1355  ACCGGC               [GA]^CCGG[CT]
EagI         1965  CGGCCG               C^GGCCG
EclXI        1965  CGGCCG               C^GGCCG
Eco47III      524  AGCGCT               AGC^GCT
Eco52I       1965  CGGCCG               C^GGCCG
EcoO65I      2166  GGTGACC              G^GT[ACGT]ACC
FbaI         1385  TGATCA               T^GATCA
HincII       1809  GTTGAC               GT[CT]^[GA]AC
HindII       1809  GTTGAC               GT[CT]^[GA]AC
KasI         1011  GGCGCC               G^GCGCC
MaeI          241  CTAG                 C^TAG
MflI          971  AGATCT               [GA]^GATC[CT]
MluNI         926  TGGCCA               TGG^CCA
MscI          926  TGGCCA               TGG^CCA
MseI          793  TTAA                 T^TAA
NarI         1011  GGCGCC               GG^CGCC
NciI          111  CCCGG                CC^[GC]GG
SapI         2097  GCTCTTC              GCTCTTC(1/4)
SbfI         1731  CCTGCAGG             CCTGCA^GG
SfoI         1011  GGCGCC               GGC^GCC
SphI         1815  GCATGC               GCATG^C
Sse8387I     1731  CCTGCAGG             CCTGCA^GG
SspBI        1595  TGTACA               T^GTACA
StuI          290  AGGCCT               AGG^CCT
TaqI          300  TCGA                 T^CGA
Tru9I         793  TTAA                 T^TAA
TthHB8I       300  TCGA                 T^CGA
XcmI          363  CCAGTTCTCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         971  AGATCT               [GA]^GATC[CT]
XmnI          423  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1397  TCCGGA               T^CCGGA
             1898  TCCGGA              
AcsI         1556  AAATTT               [GA]^AATT[CT]
             1590  GAATTT              
ApoI         1556  AAATTT               [GA]^AATT[CT]
             1590  GAATTT              
AvaI           82  CCCGAG               C^[CT]CG[GA]G
              779  CTCGGG              
BanI          193  GGTGCC               G^G[CT][GA]CC
             1011  GGCGCC              
BsaWI        1397  TCCGGA               [AT]^CCGG[AT]
             1898  TCCGGA              
BseAI        1397  TCCGGA               T^CCGGA
             1898  TCCGGA              
BsgI          212  CTGCAC               GTGCAG(16/14)
             1220  CTGCAC              
BsmI         1680  GAATGC               GAATGC(1/-1)
              296  GCATTC              
BsoBI          82  CCCGAG               C^[CT]CG[GA]G
              779  CTCGGG              
BspEI        1397  TCCGGA               T^CCGGA
             1898  TCCGGA              
BsrDI        1989  CATTGC               GCAATG(2/0)
              737  GCAATG              
BssSI         882  CACGAG               CACGAG(-5/-1)
             2155  CACGAG              
BstAPI        897  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2061  GCAGCAACTGC         
CpoI         1399  CGGACCG              CG^G[AT]CCG
             2132  CGGTCCG             
EaeI          926  TGGCCA               [CT]^GGCC[GA]
             1965  CGGCCG              
EarI         1333  CTCTTC               CTCTTC(1/4)
             2098  CTCTTC              
EcoNI         282  CCTTACAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              878  CCTCCACGAGG         
HaeII         524  AGCGCT               [GA]GCGC^[CT]
             1011  GGCGCC              
Ksp632I      1333  CTCTTC               CTCTTC(1/4)
             2098  CTCTTC              
MaeII           5  ACGT                 A^CGT
             1348  ACGT                
MroI         1397  TCCGGA               T^CCGGA
             1898  TCCGGA              
NcoI          119  CCATGG               C^CATGG
             1019  CCATGG              
NspI         1583  ACATGT               [GA]CATG^[CT]
             1815  GCATGC              
PflMI        1019  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1971  CCAGACGCTGG         
PpuMI         673  GGGACCT              [GA]G^G[AT]CC[CT]
             1327  GGGTCCC             
RsrII        1399  CGGACCG              CG^G[AT]CCG
             2132  CGGTCCG             
SacI         1123  GAGCTC               GAGCT^C
             2148  GAGCTC              
SexAI        1175  ACCTGGT              A^CC[AT]GGT
             1940  ACCAGGT             
SmlI         1030  CTTGAG               C^T[CT][GA]AG
             1194  CTCAAG              
Van91I       1019  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1971  CCAGACGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaII       BanII       BbsI        
BbvI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       
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