Restriction map for pF1KSDA0537
 (Circular) (Six-base) MAPSORT of: pF1KSDA0537.seq  Check: 9587  from: 1  to: 5122



pF1KSDA0537  5122 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AccI GT'mk_AC

Cuts at:   2072    2158    2072 
   Size:         86    5036
 
Acc65I G'GTAC_C

Cuts at:   2006    2136    2006 
   Size:        130    4992
 
AclI AA'CG_TT

Cuts at:    757    4962     757 
   Size:       4205     917
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1249    1348    2020    2945    3377    4126    4394    1249 
   Size:         99     672     925     432     749     268    1977

  Fragments arranged by size: 

                1977     925     749     672     432     268      99
 
AfeI AGC'GCT

Cuts at:   2084    2084 
   Size:       5122
 
AflIII A'CryG_T

Cuts at:   2472    3578    4555    2472 
   Size:       1106     977    3039

  Fragments arranged by size: 

                3039    1106     977
 
AgeI A'CCGG_T

Cuts at:   3510    3510 
   Size:       5122
 
AhdI GACnn_n'nnGTC

Cuts at:   1095    1095 
   Size:       5122
 
AlwNI CAG_nnn'CTG

Cuts at:   1282    1786    1998    2425    3994    1282 
   Size:        504     212     427    1569    2410

  Fragments arranged by size: 

                2410    1569     504     427     212
 
ApaI G_GGCC'C

Cuts at:    840     840 
   Size:       5122
 
ApaLI G'TGCA_C

Cuts at:    622    3892     622 
   Size:       3270    1852
 
ApoI r'AATT_y

Cuts at:   2124    4475    4731    2124 
   Size:       2351     256    2515

  Fragments arranged by size: 

                2515    2351     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5122
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5122
 
AvaI C'yCGr_G

Cuts at:    194     962    2140     194 
   Size:        768    1178    3176

  Fragments arranged by size: 

                3176    1178     768
 
BamHI G'GATC_C

Cuts at:   1322    2145    1322 
   Size:        823    4299
 
BanI G'GyrC_C

Cuts at:    180     316    1127    1473    1726    2006    2136    2550    2798 
   Size:        136     811     346     253     280     130     414     248

Cuts at:   2798    2833     180 
   Size:         35    2469

  Fragments arranged by size: 

                2469     811     414     346     280     253     248     136
                 130      35
 
BanII G_rGCy'C

Cuts at:    190     233     840    1973    2134    3164     190 
   Size:         43     607    1133     161    1030    2148

  Fragments arranged by size: 

                2148    1133    1030     607     161      43
 
BbeI G_GCGC'C

Cuts at:    184    2554    2802     184 
   Size:       2370     248    2504

  Fragments arranged by size: 

                2504    2370     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1577    1577 
   Size:       5122
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1879    1913    1879 
   Size:         34    5088
 
BciVI GTATCCnnnnn_n'

Cuts at:   3018    3781    5122    3018 
   Size:        763    1341    3018

  Fragments arranged by size: 

                3018    1341     763
 
BclI T'GATC_A

Cuts at:    512    2640     512 
   Size:       2128    2994
 
BfrBI ATG'CAT

Cuts at:    781     781 
   Size:       5122
 
BglII A'GATC_T

Cuts at:   2090    2635    4674    2090 
   Size:        545    2039    2538

  Fragments arranged by size: 

                2538    2039     545
 
BlpI GC'TnA_GC

Cuts at:   2232    2232 
   Size:       5122
 
Bme1580I G_kGCm'C

Cuts at:    626     840    1729    2745    2838    3896     626 
   Size:        214     889    1016      93    1058    1852

  Fragments arranged by size: 

                1852    1058    1016     889     214      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1056    1986    2332    2413    2748    4894    1056 
   Size:        930     346      81     335    2146    1284

  Fragments arranged by size: 

                2146    1284     930     346     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1371    1824    3487    1371 
   Size:        453    1663    3006

  Fragments arranged by size: 

                3006    1663     453
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    247    1933    1957    2107     247 
   Size:       1686      24     150    3262

  Fragments arranged by size: 

                3262    1686     150      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2296    3175    3669    3967    2296 
   Size:        879     494     298    3451

  Fragments arranged by size: 

                3451     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    295     580     798    4826     295 
   Size:        285     218    4028     591

  Fragments arranged by size: 

                4028     591     285     218
 
BsaAI yAC'GTr

Cuts at:   2112    2473    2112 
   Size:        361    4761
 
BsaBI GATnn'nnATC

Cuts at:      6     439     511    2634    4673       6 
   Size:        433      72    2123    2039     455

  Fragments arranged by size: 

                2123    2039     455     433      72
 
BsaHI Gr'CG_yC

Cuts at:    181     570    2551    2799    3503    4999     181 
   Size:        389    1981     248     704    1496     304

  Fragments arranged by size: 

                1981    1496     704     389     304     248
 
BsaWI w'CCGG_w

Cuts at:   2009    2309    2830    3510    3784    3931    4342    2009 
   Size:        300     521     680     274     147     411    2789

  Fragments arranged by size: 

                2789     680     521     411     300     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1660    1690    1660 
   Size:         30    5092
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1438    2032    1438 
   Size:        594    4528
 
BseYI C'CCAG_C

Cuts at:    174     833    1538    1636    1650    1737    2545    3303    3882 
   Size:        659     705      98      14      87     808     758     579

Cuts at:   3882    4378     174 
   Size:        496     918

  Fragments arranged by size: 

                 918     808     758     705     659     579     496      98
                  87      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1452    1452 
   Size:       5122
 
BsiEI CG_ry'CG

Cuts at:    104    1002    1225    2662    2708    3918     104 
   Size:        898     223    1437      46    1210    1308

  Fragments arranged by size: 

                1437    1308    1210     898     223      46
 
BsiHKAI G_wGCw'C

Cuts at:    626    1448    2134    2912    3102    3896     626 
   Size:        822     686     778     190     794    1852

  Fragments arranged by size: 

                1852     822     794     778     686     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    618    1935     618 
   Size:       1317    3805
 
Bsp1286I G_dGCh'C

Cuts at:    190     233     626     840    1448    1729    1973    2134    2745 
   Size:         43     393     214     608     281     244     161     611

Cuts at:   2745    2838    2912    3102    3164    3896     190 
   Size:         93      74     190      62     732    1416

  Fragments arranged by size: 

                1416     732     611     608     393     281     244     214
                 190     161      93      74      62      43
 
BspEI T'CCGG_A

Cuts at:   4342    4342 
   Size:       5122
 
BspHI T'CATG_A

Cuts at:   4298    4298 
   Size:       5122
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2170    2384    2686    3067    2170 
   Size:        214     302     381    4225

  Fragments arranged by size: 

                4225     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     564    1215    3412    3466    4952    5117      50 
   Size:        514     651    2197      54    1486     165      55

  Fragments arranged by size: 

                2197    1486     651     514     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1022    3032    1022 
   Size:       2010    3112
 
BsrFI r'CCGG_y

Cuts at:   1979    2015    3118    3299    3510    1979 
   Size:         36    1103     181     211    3591

  Fragments arranged by size: 

                3591    1103     211     181      36
 
BssSI C'ACGA_G

Cuts at:   3391    3751    3391 
   Size:        360    4762
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2497    2497 
   Size:       5122
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       5122
 
BstEII G'GTnAC_C

Cuts at:   1659    1659 
   Size:       5122
 
BstYI r'GATC_y

Cuts at:      1    1322    1952    2090    2145    2612    2635    2970    3216 
   Size:       1321     630     138      55     467      23     335     246

Cuts at:   3216    4230    4316    4328    4674       1 
   Size:       1014      86      12     346     449

  Fragments arranged by size: 

                1321    1014     630     467     449     346     335     246
                 138      86      55      23      12
 
BtgI C'CryG_G

Cuts at:    125    1730     125 
   Size:       1605    3517
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1336    1859    3070    3251    3414    1336 
   Size:        523    1211     181     163    3044

  Fragments arranged by size: 

                3044    1211     523     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1283    1584    2363    1283 
   Size:        301     779    4042

  Fragments arranged by size: 

                4042     779     301
 
DraI TTT'AAA

Cuts at:   2119    2119 
   Size:       5122
 
DraIII CAC_nnn'GTG

Cuts at:    249     249 
   Size:       5122
 
DrdI GACnn_nn'nnGTC

Cuts at:   2826    3686    2826 
   Size:        860    4262
 
EaeI y'GGCC_r

Cuts at:    357    1181    1502    2705    2879    3270    3297    4644     357 
   Size:        824     321    1203     174     391      27    1347     835

  Fragments arranged by size: 

                1347    1203     835     824     391     321     174      27
            
 
EagI C'GGCC_G

Cuts at:   2705    2705 
   Size:       5122
 
EarI CTCTTCn'nnn_

Cuts at:   1569    3143    3353    1569 
   Size:       1574     210    3338

  Fragments arranged by size: 

                3338    1574     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3328    3640    3786    4932    3328 
   Size:        312     146    1146    3518

  Fragments arranged by size: 

                3518    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   2132    2132 
   Size:       5122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247    1249    1348    1933    1957    2020    2107    2945    3377 
   Size:       1002      99     585      24      63      87     838     432

Cuts at:   3377    4126    4394     247 
   Size:        749     268     975

  Fragments arranged by size: 

                1002     975     838     749     585     432     268      99
                  87      63      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:    812    1683     812 
   Size:        871    4251
 
EcoO109I rG'GnC_Cy

Cuts at:    837    1176    1803    2259     837 
   Size:        339     627     456    3700

  Fragments arranged by size: 

                3700     627     456     339
 
EcoRI G'AATT_C

Cuts at:   2124    2124 
   Size:       5122
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    291    1713    3139     291 
   Size:       1422    1426    2274

  Fragments arranged by size: 

                2274    1426    1422
 
FspI TGC'GCA

Cuts at:   2901    2901 
   Size:       5122
 
HaeII r_GCGC'y

Cuts at:    184    2086    2554    2802    3826    4805     184 
   Size:       1902     468     248    1024     979     501

  Fragments arranged by size: 

                1902    1024     979     501     468     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    430     462    1081    1113    2962    2992    2994    3024     430 
   Size:         32     619      32    1849      30       2      30    2528

  Fragments arranged by size: 

                2528    1849     619      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2159    2159 
   Size:       5122
 
KasI G'GCGC_C

Cuts at:    180    2550    2798     180 
   Size:       2370     248    2504

  Fragments arranged by size: 

                2504    2370     248
 
KpnI G_GTAC'C

Cuts at:   2010    2140    2010 
   Size:        130    4992
 
MluI A'CGCG_T

Cuts at:   4555    4555 
   Size:       5122
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    749    1577    3793    3977     749 
   Size:        828    2216     184    1894

  Fragments arranged by size: 

                2216    1894     828     184
 
MscI TGG'CCA

Cuts at:   1183    2881    1183 
   Size:       1698    3424
 
MslI CAynn'nnrTG

Cuts at:   2050    3109    3236    2050 
   Size:       1059     127    3936

  Fragments arranged by size: 

                3936    1059     127
 
MspA1I CmG'CkG

Cuts at:    833     926    1221    1723    1925    2231    2545    2905    3920 
   Size:         93     295     502     202     306     314     360    1015

Cuts at:   3920    4165     833 
   Size:        245    1790

  Fragments arranged by size: 

                1790    1015     502     360     314     306     295     245
                 202      93
 
NaeI GCC'GGC

Cuts at:   3301    3301 
   Size:       5122
 
NarI GG'CG_CC

Cuts at:    181    2551    2799     181 
   Size:       2370     248    2504

  Fragments arranged by size: 

                2504    2370     248
 
NcoI C'CATG_G

Cuts at:    125    1730     125 
   Size:       1605    3517
 
NgoMIV G'CCGG_C

Cuts at:   3299    3299 
   Size:       5122
 
NruI TCG'CGA

Cuts at:   3483    3483 
   Size:       5122
 
NsiI A_TGCA'T

Cuts at:    783     783 
   Size:       5122
 
NspI r_CATG'y

Cuts at:   2173    2673    3582    2173 
   Size:        500     909    3713

  Fragments arranged by size: 

                3713     909     500
 
PasI CC'CwG_GG

Cuts at:    306     840     306 
   Size:        534    4588
 
PciI A'CATG_T

Cuts at:   3578    3578 
   Size:       5122
 
PflMI CCAn_nnn'nTGG

Cuts at:   1034    1034 
   Size:       5122
 
PfoI T'CCnGG_A

Cuts at:   1810    1955    1810 
   Size:        145    4977
 
PmeI GTTT'AAAC

Cuts at:   2119    2119 
   Size:       5122
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4261    4293    4261 
   Size:         32    5090
 
PshAI GACnn'nnGTC

Cuts at:   2068    2068 
   Size:       5122
 
PspOMI G'GGCC_C

Cuts at:    836     836 
   Size:       5122
 
PspXI vC'TCGA_Gb

Cuts at:    962     962 
   Size:       5122
 
PstI C_TGCA'G

Cuts at:    222     299    1151    1938    2098    2167    2852     222 
   Size:         77     852     787     160      69     685    2492

  Fragments arranged by size: 

                2492     852     787     685     160      77      69
 
PvuI CG_AT'CG

Cuts at:    104    1002     104 
   Size:        898    4224
 
PvuII CAG'CTG

Cuts at:    833    1925    2545    2905     833 
   Size:       1092     620     360    3050

  Fragments arranged by size: 

                3050    1092     620     360
 
RsrII CG'GwC_CG

Cuts at:   3315    3315 
   Size:       5122
 
SacI G_AGCT'C

Cuts at:   2134    2134 
   Size:       5122
 
SalI G'TCGA_C

Cuts at:   2157    2157 
   Size:       5122
 
SapI GCTCTTCn'nnn_

Cuts at:   3143    3353    3143 
   Size:        210    4912
 
SbfI CC_TGCA'GG

Cuts at:   2167    2167 
   Size:       5122
 
ScaI AGT'ACT

Cuts at:   4639    4639 
   Size:       5122
 
SfcI C'TryA_G

Cuts at:     33     218     295    1147    1818    1934    2094    2163    2848 
   Size:        185      77     852     671     116     160      69     685

Cuts at:   2848    3843    4034      33 
   Size:        995     191    1121

  Fragments arranged by size: 

                1121     995     852     685     671     191     185     160
                 116      77      69
 
SfoI GGC'GCC

Cuts at:    182    2552    2800     182 
   Size:       2370     248    2504

  Fragments arranged by size: 

                2504    2370     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5122
 
SmaI CCC'GGG

Cuts at:   2142    2142 
   Size:       5122
 
SmlI C'TyrA_G

Cuts at:    962    2275    3190    3684    3946     962 
   Size:       1313     915     494     262    2138

  Fragments arranged by size: 

                2138    1313     915     494     262
 
SnaBI TAC'GTA

Cuts at:   2112    2112 
   Size:       5122
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5122
 
SphI G_CATG'C

Cuts at:   2173    2673    2173 
   Size:        500    4622
 
SspI AAT'ATT

Cuts at:    699     699 
   Size:       5122
 
StyI C'CwwG_G

Cuts at:    125    1316    1730    2254    2608     125 
   Size:       1191     414     524     354    2639

  Fragments arranged by size: 

                2639    1191     524     414     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    832    1029    2703    2836    3295    3506     832 
   Size:        197    1674     133     459     211    2448

  Fragments arranged by size: 

                2448    1674     459     211     197     133
 
TatI w'GTAC_w

Cuts at:   4637    4637 
   Size:       5122
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1120    1152    1660    1692    4639    4671    1120 
   Size:         32     508      32    2947      32    1571

  Fragments arranged by size: 

                2947    1571     508      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2917    2917 
   Size:       5122
 
XbaI T'CTAG_A

Cuts at:   2151    2151 
   Size:       5122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2615    2615 
   Size:       5122
 
XhoI C'TCGA_G

Cuts at:    962     962 
   Size:       5122
 
XmaI C'CCGG_G

Cuts at:   2140    2140 
   Size:       5122
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     AlfI     AloI     AscI    AvrII
     BaeI    BbvCI     BglI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI
    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     PacI     PmlI    PpuMI     PsiI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI     SrfI     StuI     SwaI     XmnI     ZraI
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