Restriction map for pF1KSDA0537
 (Linear) (Six-base) MAPSORT of: KIAA0537.seq  Check: 2815  from: 1  to: 1983



KIAA0537.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AccI GT'mk_AC

Cuts at:      0    1946    1983 
   Size:       1946      37
 
Acc65I G'GTAC_C

Cuts at:      0    1880    1983 
   Size:       1880     103
 
AclI AA'CG_TT

Cuts at:      0     631    1983 
   Size:        631    1352
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1222    1894    1983 
   Size:       1123      99     672      89

  Fragments arranged by size: 

                1123     672      99      89
 
AfeI AGC'GCT

Cuts at:      0    1958    1983 
   Size:       1958      25
 
AhdI GACnn_n'nnGTC

Cuts at:      0     969    1983 
   Size:        969    1014
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1156    1660    1872    1983 
   Size:       1156     504     212     111

  Fragments arranged by size: 

                1156     504     212     111
 
ApaI G_GGCC'C

Cuts at:      0     714    1983 
   Size:        714    1269
 
ApaLI G'TGCA_C

Cuts at:      0     496    1983 
   Size:        496    1487
 
AvaI C'yCGr_G

Cuts at:      0      68     836    1983 
   Size:         68     768    1147

  Fragments arranged by size: 

                1147     768      68
 
BamHI G'GATC_C

Cuts at:      0    1196    1983 
   Size:       1196     787
 
BanI G'GyrC_C

Cuts at:      0      54     190    1001    1347    1600    1880    1983 
   Size:         54     136     811     346     253     280     103

  Fragments arranged by size: 

                 811     346     280     253     136     103      54
 
BanII G_rGCy'C

Cuts at:      0      64     107     714    1847    1983 
   Size:         64      43     607    1133     136

  Fragments arranged by size: 

                1133     607     136      64      43
 
BbeI G_GCGC'C

Cuts at:      0      58    1983 
   Size:         58    1925
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1451    1983 
   Size:       1451     532
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1753    1787    1983 
   Size:       1753      34     196

  Fragments arranged by size: 

                1753     196      34
 
BclI T'GATC_A

Cuts at:      0     386    1983 
   Size:        386    1597
 
BfrBI ATG'CAT

Cuts at:      0     655    1983 
   Size:        655    1328
 
BglII A'GATC_T

Cuts at:      0    1964    1983 
   Size:       1964      19
 
Bme1580I G_kGCm'C

Cuts at:      0     500     714    1603    1983 
   Size:        500     214     889     380

  Fragments arranged by size: 

                 889     500     380     214
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     930    1860    1983 
   Size:        930     930     123

  Fragments arranged by size: 

                 930     930     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1245    1698    1983 
   Size:       1245     453     285

  Fragments arranged by size: 

                1245     453     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1807    1831    1981    1983 
   Size:        121    1686      24     150       2

  Fragments arranged by size: 

                1686     150     121      24       2
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169     454     672    1983 
   Size:        169     285     218    1311

  Fragments arranged by size: 

                1311     285     218     169
 
BsaBI GATnn'nnATC

Cuts at:      0     313     385    1983 
   Size:        313      72    1598

  Fragments arranged by size: 

                1598     313      72
 
BsaHI Gr'CG_yC

Cuts at:      0      55     444    1983 
   Size:         55     389    1539

  Fragments arranged by size: 

                1539     389      55
 
BsaWI w'CCGG_w

Cuts at:      0    1883    1983 
   Size:       1883     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1534    1564    1983 
   Size:       1534      30     419

  Fragments arranged by size: 

                1534     419      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1312    1906    1983 
   Size:       1312     594      77

  Fragments arranged by size: 

                1312     594      77
 
BseYI C'CCAG_C

Cuts at:      0      48     707    1412    1510    1524    1611    1983 
   Size:         48     659     705      98      14      87     372

  Fragments arranged by size: 

                 705     659     372      98      87      48      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1326    1983 
   Size:       1326     657
 
BsiEI CG_ry'CG

Cuts at:      0     876    1099    1983 
   Size:        876     223     884

  Fragments arranged by size: 

                 884     876     223
 
BsiHKAI G_wGCw'C

Cuts at:      0     500    1322    1983 
   Size:        500     822     661

  Fragments arranged by size: 

                 822     661     500
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     492    1809    1983 
   Size:        492    1317     174

  Fragments arranged by size: 

                1317     492     174
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     107     500     714    1322    1603    1847    1983 
   Size:         64      43     393     214     608     281     244     136

  Fragments arranged by size: 

                 608     393     281     244     214     136      64      43
            
 
BsrBI CCG'CTC

Cuts at:      0     438    1089    1983 
   Size:        438     651     894

  Fragments arranged by size: 

                 894     651     438
 
BsrDI GCAATG_nn'

Cuts at:      0     896    1983 
   Size:        896    1087
 
BsrFI r'CCGG_y

Cuts at:      0    1853    1889    1983 
   Size:       1853      36      94

  Fragments arranged by size: 

                1853      94      36
 
BstEII G'GTnAC_C

Cuts at:      0    1533    1983 
   Size:       1533     450
 
BstYI r'GATC_y

Cuts at:      0    1196    1826    1964    1983 
   Size:       1196     630     138      19

  Fragments arranged by size: 

                1196     630     138      19
 
BtgI C'CryG_G

Cuts at:      0    1604    1983 
   Size:       1604     379
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1210    1733    1983 
   Size:       1210     523     250

  Fragments arranged by size: 

                1210     523     250
 
BtsI GCAGTG_nn'

Cuts at:      0    1157    1458    1983 
   Size:       1157     301     525

  Fragments arranged by size: 

                1157     525     301
 
DraIII CAC_nnn'GTG

Cuts at:      0     123    1983 
   Size:        123    1860
 
EaeI y'GGCC_r

Cuts at:      0     231    1055    1376    1983 
   Size:        231     824     321     607

  Fragments arranged by size: 

                 824     607     321     231
 
EarI CTCTTCn'nnn_

Cuts at:      0    1443    1983 
   Size:       1443     540
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1123    1222    1807    1831    1894    1981    1983 
   Size:        121    1002      99     585      24      63      87       2

  Fragments arranged by size: 

                1002     585     121      99      87      63      24       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     686    1557    1983 
   Size:        686     871     426

  Fragments arranged by size: 

                 871     686     426
 
EcoO109I rG'GnC_Cy

Cuts at:      0     711    1050    1677    1983 
   Size:        711     339     627     306

  Fragments arranged by size: 

                 711     627     339     306
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     165    1587    1983 
   Size:        165    1422     396

  Fragments arranged by size: 

                1422     396     165
 
HaeII r_GCGC'y

Cuts at:      0      58    1960    1983 
   Size:         58    1902      23

  Fragments arranged by size: 

                1902      58      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336     955     987    1983 
   Size:        304      32     619      32     996

  Fragments arranged by size: 

                 996     619     304      32      32
 
KasI G'GCGC_C

Cuts at:      0      54    1983 
   Size:         54    1929
 
KpnI G_GTAC'C

Cuts at:      0    1884    1983 
   Size:       1884      99
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    1451    1983 
   Size:        623     828     532

  Fragments arranged by size: 

                 828     623     532
 
MscI TGG'CCA

Cuts at:      0    1057    1983 
   Size:       1057     926
 
MslI CAynn'nnrTG

Cuts at:      0    1924    1983 
   Size:       1924      59
 
MspA1I CmG'CkG

Cuts at:      0     707     800    1095    1597    1799    1983 
   Size:        707      93     295     502     202     184

  Fragments arranged by size: 

                 707     502     295     202     184      93
 
NarI GG'CG_CC

Cuts at:      0      55    1983 
   Size:         55    1928
 
NcoI C'CATG_G

Cuts at:      0    1604    1983 
   Size:       1604     379
 
NsiI A_TGCA'T

Cuts at:      0     657    1983 
   Size:        657    1326
 
PasI CC'CwG_GG

Cuts at:      0     180     714    1983 
   Size:        180     534    1269

  Fragments arranged by size: 

                1269     534     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     908    1983 
   Size:        908    1075
 
PfoI T'CCnGG_A

Cuts at:      0    1684    1829    1983 
   Size:       1684     145     154

  Fragments arranged by size: 

                1684     154     145
 
PshAI GACnn'nnGTC

Cuts at:      0    1942    1983 
   Size:       1942      41
 
PspOMI G'GGCC_C

Cuts at:      0     710    1983 
   Size:        710    1273
 
PspXI vC'TCGA_Gb

Cuts at:      0     836    1983 
   Size:        836    1147
 
PstI C_TGCA'G

Cuts at:      0      96     173    1025    1812    1972    1983 
   Size:         96      77     852     787     160      11

  Fragments arranged by size: 

                 852     787     160      96      77      11
 
PvuI CG_AT'CG

Cuts at:      0     876    1983 
   Size:        876    1107
 
PvuII CAG'CTG

Cuts at:      0     707    1799    1983 
   Size:        707    1092     184

  Fragments arranged by size: 

                1092     707     184
 
SfcI C'TryA_G

Cuts at:      0      92     169    1021    1692    1808    1968    1983 
   Size:         92      77     852     671     116     160      15

  Fragments arranged by size: 

                 852     671     160     116      92      77      15
 
SfoI GGC'GCC

Cuts at:      0      56    1983 
   Size:         56    1927
 
SmlI C'TyrA_G

Cuts at:      0     836    1983 
   Size:        836    1147
 
SspI AAT'ATT

Cuts at:      0     573    1983 
   Size:        573    1410
 
StyI C'CwwG_G

Cuts at:      0    1190    1604    1983 
   Size:       1190     414     379

  Fragments arranged by size: 

                1190     414     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     706     903    1983 
   Size:        706     197    1080

  Fragments arranged by size: 

                1080     706     197
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     994    1026    1534    1566    1983 
   Size:        994      32     508      32     417

  Fragments arranged by size: 

                 994     508     417      32      32
 
XhoI C'TCGA_G

Cuts at:      0     836    1983 
   Size:        836    1147
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI     AhdI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
     BclI    BfrBI    BglII Bme1580I     BmrI     BplI     BpmI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI   BstEII    BstYI
     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NsiI     PasI    PflMI     PfoI
    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII     SfcI     SfoI
     SmlI     SspI     StyI    TaqII     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII    AflII   AflIII     AgeI     AleI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI
     BglI     BlpI    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI     PsiI     PsrI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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