Restriction map for pF1KSDA0448
 (Circular) (Six-base) MAPSORT of: pF1KSDA0448.seq  Check: 3746  from: 1  to: 4207



pF1KSDA0448  4207 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    815     815 
   Size:       4207
 
AccI GT'mk_AC

Cuts at:   1243    1243 
   Size:       4207
 
Acc65I G'GTAC_C

Cuts at:   1221    1221 
   Size:       4207
 
AclI AA'CG_TT

Cuts at:   4047    4047 
   Size:       4207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    908    2030    2462    3211    3479     908 
   Size:       1122     432     749     268    1636

  Fragments arranged by size: 

                1636    1122     749     432     268
 
AflIII A'CryG_T

Cuts at:    549    1557    2663    3640     549 
   Size:       1008    1106     977    1116

  Fragments arranged by size: 

                1116    1106    1008     977
 
AgeI A'CCGG_T

Cuts at:   2595    2595 
   Size:       4207
 
AhdI GACnn_n'nnGTC

Cuts at:    271     271 
   Size:       4207
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1054    1086    1054 
   Size:         32    4175
 
AlwNI CAG_nnn'CTG

Cuts at:    168     225    1510    3079     168 
   Size:         57    1285    1569    1296

  Fragments arranged by size: 

                1569    1296    1285      57
 
ApaLI G'TGCA_C

Cuts at:   2977    2977 
   Size:       4207
 
ApoI r'AATT_y

Cuts at:    574    1078    1209    3560    3816     574 
   Size:        504     131    2351     256     965

  Fragments arranged by size: 

                2351     965     504     256     131
 
AseI AT'TA_AT

Cuts at:     20     605     860      20 
   Size:        585     255    3367

  Fragments arranged by size: 

                3367     585     255
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4207
 
AvaI C'yCGr_G

Cuts at:   1225    1225 
   Size:       4207
 
BamHI G'GATC_C

Cuts at:    618    1230     618 
   Size:        612    3595
 
BanI G'GyrC_C

Cuts at:   1221    1635    1883    1918    1221 
   Size:        414     248      35    3510

  Fragments arranged by size: 

                3510     414     248      35
 
BanII G_rGCy'C

Cuts at:    134    1113    1219    2249     134 
   Size:        979     106    1030    2092

  Fragments arranged by size: 

                2092    1030     979     106
 
BbeI G_GCGC'C

Cuts at:   1639    1887    1639 
   Size:        248    3959
 
BciVI GTATCCnnnnn_n'

Cuts at:   2103    2866    4207    2103 
   Size:        763    1341    2103

  Fragments arranged by size: 

                2103    1341     763
 
BclI T'GATC_A

Cuts at:    350    1725     350 
   Size:       1375    2832
 
BglII A'GATC_T

Cuts at:   1720    3759    1720 
   Size:       2039    2168
 
BlpI GC'TnA_GC

Cuts at:   1317    1317 
   Size:       4207
 
Bme1580I G_kGCm'C

Cuts at:   1830    1923    2981    1830 
   Size:         93    1058    3056

  Fragments arranged by size: 

                3056    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1417    1498    1833    3979    1417 
   Size:         81     335    2146    1645

  Fragments arranged by size: 

                2146    1645     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    255    2572     255 
   Size:       2317    1890
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     740     247 
   Size:        493    3714
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1381    2260    2754    3052    1381 
   Size:        879     494     298    2536

  Fragments arranged by size: 

                2536     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1130    3911    1130 
   Size:       2781    1426
 
BsaAI yAC'GTr

Cuts at:   1197    1558    1197 
   Size:        361    3846
 
BsaBI GATnn'nnATC

Cuts at:      6    1719    3758       6 
   Size:       1713    2039     455

  Fragments arranged by size: 

                2039    1713     455
 
BsaHI Gr'CG_yC

Cuts at:   1636    1884    2588    4084    1636 
   Size:        248     704    1496    1759

  Fragments arranged by size: 

                1759    1496     704     248
 
BsaWI w'CCGG_w

Cuts at:   1394    1915    2595    2869    3016    3427    1394 
   Size:        521     680     274     147     411    2174

  Fragments arranged by size: 

                2174     680     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1054    1084    1054 
   Size:         30    4177
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    124     244     124 
   Size:        120    4087
 
BseYI C'CCAG_C

Cuts at:   1630    2388    2967    3463    1630 
   Size:        758     579     496    2374

  Fragments arranged by size: 

                2374     758     579     496
 
BsiEI CG_ry'CG

Cuts at:    104    1747    1793    3003     104 
   Size:       1643      46    1210    1308

  Fragments arranged by size: 

                1643    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    756    1219    1997    2187    2981     756 
   Size:        463     778     190     794    1982

  Fragments arranged by size: 

                1982     794     778     463     190
 
BsmI GAATG_Cn'

Cuts at:    811     811 
   Size:       4207
 
BsmBI CGTCTCn'nnnn_

Cuts at:    684     684 
   Size:       4207
 
Bsp1286I G_dGCh'C

Cuts at:    134     756    1113    1219    1830    1923    1997    2187    2249 
   Size:        622     357     106     611      93      74     190      62

Cuts at:   2249    2981     134 
   Size:        732    1360

  Fragments arranged by size: 

                1360     732     622     611     357     190     106      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3427    3427 
   Size:       4207
 
BspHI T'CATG_A

Cuts at:   3383    3383 
   Size:       4207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    815    1255    1469    1771    2152     815 
   Size:        440     214     302     381    2870

  Fragments arranged by size: 

                2870     440     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     239    2497    2551    4037    4202      50 
   Size:        189    2258      54    1486     165      55

  Fragments arranged by size: 

                2258    1486     189     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    475     925    1025    2117     475 
   Size:        450     100    1092    2565

  Fragments arranged by size: 

                2565    1092     450     100
 
BsrFI r'CCGG_y

Cuts at:   2203    2384    2595    2203 
   Size:        181     211    3815

  Fragments arranged by size: 

                3815     211     181
 
BssSI C'ACGA_G

Cuts at:   2476    2836    2476 
   Size:        360    3847
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1582    1582 
   Size:       4207
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4207
 
BstYI r'GATC_y

Cuts at:      1     215     618    1230    1697    1720    2055    2301    3315 
   Size:        214     403     612     467      23     335     246    1014

Cuts at:   3315    3401    3413    3759       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1014     612     467     449     403     346     335     246
                 214      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    136     136 
   Size:       4207
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       4207
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    208    2155    2336    2499     208 
   Size:       1947     181     163    1916

  Fragments arranged by size: 

                1947    1916     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1448    1448 
   Size:       4207
 
DraI TTT'AAA

Cuts at:   1204    1204 
   Size:       4207
 
DrdI GACnn_nn'nnGTC

Cuts at:   1911    2771    1911 
   Size:        860    3347
 
EaeI y'GGCC_r

Cuts at:    792    1790    1964    2355    2382    3729     792 
   Size:        998     174     391      27    1347    1270

  Fragments arranged by size: 

                1347    1270     998     391     174      27
 
EagI C'GGCC_G

Cuts at:   1790    1790 
   Size:       4207
 
EarI CTCTTCn'nnn_

Cuts at:    205     332    2228    2438     205 
   Size:        127    1896     210    1974

  Fragments arranged by size: 

                1974    1896     210     127
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2413    2725    2871    4017    2413 
   Size:        312     146    1146    2603

  Fragments arranged by size: 

                2603    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   1217    1217 
   Size:       4207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     740     908    2030    2462    3211    3479     247 
   Size:        493     168    1122     432     749     268     975

  Fragments arranged by size: 

                1122     975     749     493     432     268     168
 
EcoO109I rG'GnC_Cy

Cuts at:   1344    1344 
   Size:       4207
 
EcoRI G'AATT_C

Cuts at:   1209    1209 
   Size:       4207
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2224    2224 
   Size:       4207
 
FspI TGC'GCA

Cuts at:   1986    1986 
   Size:       4207
 
HaeII r_GCGC'y

Cuts at:   1639    1887    2911    3890    1639 
   Size:        248    1024     979    1956

  Fragments arranged by size: 

                1956    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    257     289    2047    2077    2079    2109     257 
   Size:         32    1758      30       2      30    2355

  Fragments arranged by size: 

                2355    1758      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1244    1244 
   Size:       4207
 
KasI G'GCGC_C

Cuts at:   1635    1883    1635 
   Size:        248    3959
 
KpnI G_GTAC'C

Cuts at:   1225    1225 
   Size:       4207
 
MluI A'CGCG_T

Cuts at:   3640    3640 
   Size:       4207
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2878    3062    2878 
   Size:        184    4023
 
MscI TGG'CCA

Cuts at:    794    1966     794 
   Size:       1172    3035
 
MslI CAynn'nnrTG

Cuts at:   2194    2321    2194 
   Size:        127    4080
 
MspA1I CmG'CkG

Cuts at:    165    1316    1630    1990    3005    3250     165 
   Size:       1151     314     360    1015     245    1122

  Fragments arranged by size: 

                1151    1122    1015     360     314     245
 
NaeI GCC'GGC

Cuts at:   2386    2386 
   Size:       4207
 
NarI GG'CG_CC

Cuts at:   1636    1884    1636 
   Size:        248    3959
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4207
 
NgoMIV G'CCGG_C

Cuts at:   2384    2384 
   Size:       4207
 
NruI TCG'CGA

Cuts at:   2568    2568 
   Size:       4207
 
NspI r_CATG'y

Cuts at:   1258    1758    2667    1258 
   Size:        500     909    2798

  Fragments arranged by size: 

                2798     909     500
 
PciI A'CATG_T

Cuts at:   2663    2663 
   Size:       4207
 
PflMI CCAn_nnn'nTGG

Cuts at:    225     225 
   Size:       4207
 
PfoI T'CCnGG_A

Cuts at:    514     514 
   Size:       4207
 
PmeI GTTT'AAAC

Cuts at:   1204    1204 
   Size:       4207
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1054    1086    3346    3378    1054 
   Size:         32    2260      32    1883

  Fragments arranged by size: 

                2260    1883      32      32
 
PsiI TTA'TAA

Cuts at:    359     359 
   Size:       4207
 
PstI C_TGCA'G

Cuts at:   1252    1937    1252 
   Size:        685    3522
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4207
 
PvuII CAG'CTG

Cuts at:    165    1630    1990     165 
   Size:       1465     360    2382

  Fragments arranged by size: 

                2382    1465     360
 
RsrII CG'GwC_CG

Cuts at:   2400    2400 
   Size:       4207
 
SacI G_AGCT'C

Cuts at:   1219    1219 
   Size:       4207
 
SalI G'TCGA_C

Cuts at:   1242    1242 
   Size:       4207
 
SapI GCTCTTCn'nnn_

Cuts at:    205    2228    2438     205 
   Size:       2023     210    1974

  Fragments arranged by size: 

                2023    1974     210
 
SbfI CC_TGCA'GG

Cuts at:   1252    1252 
   Size:       4207
 
ScaI AGT'ACT

Cuts at:   3724    3724 
   Size:       4207
 
SfcI C'TryA_G

Cuts at:     33    1036    1248    1933    2928    3119      33 
   Size:       1003     212     685     995     191    1121

  Fragments arranged by size: 

                1121    1003     995     685     212     191
 
SfoI GGC'GCC

Cuts at:   1637    1885    1637 
   Size:        248    3959
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4207
 
SmaI CCC'GGG

Cuts at:   1227    1227 
   Size:       4207
 
SmlI C'TyrA_G

Cuts at:   1360    2275    2769    3031    1360 
   Size:        915     494     262    2536

  Fragments arranged by size: 

                2536     915     494     262
 
SnaBI TAC'GTA

Cuts at:   1197    1197 
   Size:       4207
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4207
 
SphI G_CATG'C

Cuts at:   1258    1758    1258 
   Size:        500    3707
 
SspI AAT'ATT

Cuts at:    868     868 
   Size:       4207
 
StyI C'CwwG_G

Cuts at:    125    1339    1693     125 
   Size:       1214     354    2639

  Fragments arranged by size: 

                2639    1214     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1788    1921    2380    2591    1788 
   Size:        133     459     211    3404

  Fragments arranged by size: 

                3404     459     211     133
 
TatI w'GTAC_w

Cuts at:   3722    3722 
   Size:       4207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3724    3756    3724 
   Size:         32    4175
 
Tth111I GACn'n_nGTC

Cuts at:   2002    2002 
   Size:       4207
 
XbaI T'CTAG_A

Cuts at:   1236    1236 
   Size:       4207
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    764    1700     764 
   Size:        936    3271
 
XmaI C'CCGG_G

Cuts at:   1225    1225 
   Size:       4207
 
XmnI GAAnn'nnTTC

Cuts at:    869    1069     869 
   Size:        200    4007
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NruI     NspI     PciI    PflMI     PfoI     PmeI     PpiI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I     BsgI    BsiWI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NsiI     PacI     PasI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI     SrfI     StuI     SwaI     XhoI     ZraI
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