Restriction map for pF1KSDA0410
 (Linear) (Six-base) MAPSORT of: KIAA0410.seq  Check: 7743  from: 1  to: 1455



KIAA0410.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     745     889    1020    1054    1192    1455 
   Size:        306     439     144     131      34     138     263

  Fragments arranged by size: 

                 439     306     263     144     138     131      34
 
AflII C'TTAA_G

Cuts at:      0     841    1455 
   Size:        841     614
 
AleI CACnn'nnGTG

Cuts at:      0      46    1455 
   Size:         46    1409
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1442    1455 
   Size:       1442      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     352     879    1024    1455 
   Size:        352     527     145     431

  Fragments arranged by size: 

                 527     431     352     145
 
ApoI r'AATT_y

Cuts at:      0     778    1172    1455 
   Size:        778     394     283

  Fragments arranged by size: 

                 778     394     283
 
BanII G_rGCy'C

Cuts at:      0      40     125     200    1455 
   Size:         40      85      75    1255

  Fragments arranged by size: 

                1255      85      75      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     818     994    1455 
   Size:        818     176     461

  Fragments arranged by size: 

                 818     461     176
 
BbvCI CC'TCA_GC

Cuts at:      0     387    1455 
   Size:        387    1068
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     230    1455 
   Size:        230    1225
 
BmtI G_CTAG'C

Cuts at:      0     395    1455 
   Size:        395    1060
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     929    1455 
   Size:        929     526
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     413     757    1455 
   Size:        140     273     344     698

  Fragments arranged by size: 

                 698     344     273     140
 
Bpu10I CC'TnA_GC

Cuts at:      0     387     522     947    1455 
   Size:        387     135     425     508

  Fragments arranged by size: 

                 508     425     387     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1103    1130    1455 
   Size:       1103      27     325

  Fragments arranged by size: 

                1103     325      27
 
BsaBI GATnn'nnATC

Cuts at:      0     734    1455 
   Size:        734     721
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     138     156     168     186     411     441    1268    1298 
   Size:        138      18      12      18     225      30     827      30

Cuts at:   1298    1455 
   Size:        157

  Fragments arranged by size: 

                 827     225     157     138      30      30      18      18
                  12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     660    1455 
   Size:        660     795
 
BsmI GAATG_Cn'

Cuts at:      0     591     966    1444    1455 
   Size:        591     375     478      11

  Fragments arranged by size: 

                 591     478     375      11
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     125     200    1455 
   Size:         40      85      75    1255

  Fragments arranged by size: 

                1255      85      75      40
 
BspHI T'CATG_A

Cuts at:      0    1218    1455 
   Size:       1218     237
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     222     351    1455 
   Size:        222     129    1104

  Fragments arranged by size: 

                1104     222     129
 
BsrDI GCAATG_nn'

Cuts at:      0     642     842    1455 
   Size:        642     200     613

  Fragments arranged by size: 

                 642     613     200
 
BsrFI r'CCGG_y

Cuts at:      0      55    1455 
   Size:         55    1400
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     352    1024    1390    1455 
   Size:        352     672     366      65

  Fragments arranged by size: 

                 672     366     352      65
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48    1455 
   Size:         48    1407
 
BstYI r'GATC_y

Cuts at:      0     205    1455 
   Size:        205    1250
 
BtgI C'CryG_G

Cuts at:      0      47    1394    1455 
   Size:         47    1347      61

  Fragments arranged by size: 

                1347      61      47
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     345     380    1455 
   Size:        345      35    1075

  Fragments arranged by size: 

                1075     345      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     408    1455 
   Size:        408    1047
 
EaeI y'GGCC_r

Cuts at:      0      50    1455 
   Size:         50    1405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     306     413     745     757     889    1020    1054 
   Size:        140     166     107     332      12     132     131      34

Cuts at:   1054    1192    1455 
   Size:        138     263

  Fragments arranged by size: 

                 332     263     166     140     138     132     131     107
                  34      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1430    1455 
   Size:       1430      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     856    1455 
   Size:        856     599
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     411     443    1455 
   Size:        411      32    1012

  Fragments arranged by size: 

                1012     411      32
 
HincII GTy'rAC

Cuts at:      0     599    1455 
   Size:        599     856
 
MslI CAynn'nnrTG

Cuts at:      0      46    1013    1223    1455 
   Size:         46     967     210     232

  Fragments arranged by size: 

                 967     232     210      46
 
NaeI GCC'GGC

Cuts at:      0      57    1455 
   Size:         57    1398
 
NcoI C'CATG_G

Cuts at:      0    1394    1455 
   Size:       1394      61
 
NgoMIV G'CCGG_C

Cuts at:      0      55    1455 
   Size:         55    1400
 
NheI G'CTAG_C

Cuts at:      0     391    1455 
   Size:        391    1064
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1322    1455 
   Size:       1322     133
 
PfoI T'CCnGG_A

Cuts at:      0      25    1455 
   Size:         25    1430
 
PstI C_TGCA'G

Cuts at:      0     309     348     633    1455 
   Size:        309      39     285     822

  Fragments arranged by size: 

                 822     309     285      39
 
ScaI AGT'ACT

Cuts at:      0     147    1455 
   Size:        147    1308
 
SfcI C'TryA_G

Cuts at:      0     269     305     344     611     629    1257    1430    1455 
   Size:        269      36      39     267      18     628     173      25

  Fragments arranged by size: 

                 628     269     267     173      39      36      25      18
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0      55    1455 
   Size:         55    1400
 
SmlI C'TyrA_G

Cuts at:      0     841    1118    1145    1455 
   Size:        841     277      27     310

  Fragments arranged by size: 

                 841     310     277      27
 
StuI AGG'CCT

Cuts at:      0     503     644    1455 
   Size:        503     141     811

  Fragments arranged by size: 

                 811     503     141
 
StyI C'CwwG_G

Cuts at:      0     645    1251    1394    1455 
   Size:        645     606     143      61

  Fragments arranged by size: 

                 645     606     143      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454    1350    1455 
   Size:        454     896     105

  Fragments arranged by size: 

                 896     454     105
 
TatI w'GTAC_w

Cuts at:      0     145     553    1076    1455 
   Size:        145     408     523     379

  Fragments arranged by size: 

                 523     408     379     145
 
XmnI GAAnn'nnTTC

Cuts at:      0     147     825    1267    1444    1455 
   Size:        147     678     442     177      11

  Fragments arranged by size: 

                 678     442     177     147      11
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI    AlwNI     ApoI    BanII     BbsI
    BbvCI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaXI    BseRI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
   BstAPI    BstXI    BstYI     BtgI    CspCI     DrdI     EaeI  Eco57MI
    EcoNI     FalI    Hin4I   HincII     MslI     NaeI     NcoI   NgoMIV
     NheI    PflMI     PfoI     PstI     ScaI     SfcI    SgrAI     SmlI
     StuI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BsaI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NarI     NdeI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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