Restriction map for pF1KSDA0387
 (Linear) (Six-base) MAPSORT of: KIAA0387.seq  Check: 8598  from: 1  to: 3114



KIAA0387.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     539    1671    3114 
   Size:        539    1132    1443

  Fragments arranged by size: 

                1443    1132     539
 
AatII G_ACGT'C

Cuts at:      0    1667    3114 
   Size:       1667    1447
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449    1021    1361    2029    2305    2455    2764    3114 
   Size:        449     572     340     668     276     150     309     350

  Fragments arranged by size: 

                 668     572     449     350     340     309     276     150
            
 
AfeI AGC'GCT

Cuts at:      0    2321    3114 
   Size:       2321     793
 
AflIII A'CryG_T

Cuts at:      0     662    2126    3114 
   Size:        662    1464     988

  Fragments arranged by size: 

                1464     988     662
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1287    3114 
   Size:       1287    1827
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2680    2712    3114 
   Size:       2680      32     402

  Fragments arranged by size: 

                2680     402      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162     312    2073    3114 
   Size:        162     150    1761    1041

  Fragments arranged by size: 

                1761    1041     162     150
 
ApaI G_GGCC'C

Cuts at:      0     692    1508    2122    3114 
   Size:        692     816     614     992

  Fragments arranged by size: 

                 992     816     692     614
 
ApaLI G'TGCA_C

Cuts at:      0     878    3114 
   Size:        878    2236
 
ApoI r'AATT_y

Cuts at:      0    1832    3114 
   Size:       1832    1282
 
AvaI C'yCGr_G

Cuts at:      0     190     685     841    1112    1264    1462    1636    2022 
   Size:        190     495     156     271     152     198     174     386

Cuts at:   2022    2423    2499    3114 
   Size:        401      76     615

  Fragments arranged by size: 

                 615     495     401     386     271     198     190     174
                 156     152      76
 
BanI G'GyrC_C

Cuts at:      0     248     909    1683    2926    3114 
   Size:        248     661     774    1243     188

  Fragments arranged by size: 

                1243     774     661     248     188
 
BanII G_rGCy'C

Cuts at:      0     692     755    1311    1463    1487    1508    2122    2345 
   Size:        692      63     556     152      24      21     614     223

Cuts at:   2345    3114 
   Size:        769

  Fragments arranged by size: 

                 769     692     614     556     223     152      63      24
                  21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     962    1358    1367    1904    3114 
   Size:        962     396       9     537    1210

  Fragments arranged by size: 

                1210     962     537     396       9
 
BbvCI CC'TCA_GC

Cuts at:      0     614    1025    1552    3077    3114 
   Size:        614     411     527    1525      37

  Fragments arranged by size: 

                1525     614     527     411      37
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1680    1714    2026    2060    3114 
   Size:       1680      34     312      34    1054

  Fragments arranged by size: 

                1680    1054     312      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1043    2335    2548    2924    3114 
   Size:       1043    1292     213     376     190

  Fragments arranged by size: 

                1292    1043     376     213     190
 
BglII A'GATC_T

Cuts at:      0    1431    3114 
   Size:       1431    1683
 
BlpI GC'TnA_GC

Cuts at:      0     754    1067    3114 
   Size:        754     313    2047

  Fragments arranged by size: 

                2047     754     313
 
Bme1580I G_kGCm'C

Cuts at:      0     692     882    1508    1688    2122    2384    3114 
   Size:        692     190     626     180     434     262     730

  Fragments arranged by size: 

                 730     692     626     434     262     190     180
 
BmgBI CAC'GTC

Cuts at:      0     573     665    1707    2129    3114 
   Size:        573      92    1042     422     985

  Fragments arranged by size: 

                1042     985     573     422      92
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2148    3114 
   Size:       2148     966
 
BmtI G_CTAG'C

Cuts at:      0    2480    3114 
   Size:       2480     634
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1836    2037    3114 
   Size:       1836     201    1077

  Fragments arranged by size: 

                1836    1077     201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     724     877    1069    1169    1325    1831    2323 
   Size:        562     162     153     192     100     156     506     492

Cuts at:   2323    3022    3114 
   Size:        699      92

  Fragments arranged by size: 

                 699     562     506     492     192     162     156     153
                 100      92
 
Bpu10I CC'TnA_GC

Cuts at:      0     614    1025    1541    1552    3077    3114 
   Size:        614     411     516      11    1525      37

  Fragments arranged by size: 

                1525     614     516     411      37      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     870    1852    2811    3030    3082    3114 
   Size:        870     982     959     219      52      32

  Fragments arranged by size: 

                 982     959     870     219      52      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1295    1616    1935    2700    3015    3114 
   Size:       1295     321     319     765     315      99

  Fragments arranged by size: 

                1295     765     321     319     315      99
 
BsaAI yAC'GTr

Cuts at:      0     353    3114 
   Size:        353    2761
 
BsaBI GATnn'nnATC

Cuts at:      0    2986    3114 
   Size:       2986     128
 
BsaHI Gr'CG_yC

Cuts at:      0     423     525    1664    1952    2624    3114 
   Size:        423     102    1139     288     672     490

  Fragments arranged by size: 

                1139     672     490     423     288     102
 
BsaWI w'CCGG_w

Cuts at:      0    1843    3114 
   Size:       1843    1271
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     471     501     535     565     616     646     666     696 
   Size:        471      30      34      30      51      30      20      30

Cuts at:    696     850     880    2296    2326    2680    2710    3114 
   Size:        154      30    1416      30     354      30     404

  Fragments arranged by size: 

                1416     471     404     354     154      51      34      30
                  30      30      30      30      30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     492    1399    1531    1871    2230    2386    2745 
   Size:        208     284     907     132     340     359     156     359

Cuts at:   2745    2814    2825    3094    3114 
   Size:         69      11     269      20

  Fragments arranged by size: 

                 907     359     359     340     284     269     208     156
                 132      69      20      11
 
BseYI C'CCAG_C

Cuts at:      0     441     561     698     831    1437    1687    2176    3114 
   Size:        441     120     137     133     606     250     489     938

  Fragments arranged by size: 

                 938     606     489     441     250     137     133     120
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1231    2066    2245    2933    3114 
   Size:        187    1044     835     179     688     181

  Fragments arranged by size: 

                1044     835     688     187     181     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     578     755     882     984    1358    1487    1542    2705 
   Size:        578     177     127     102     374     129      55    1163

Cuts at:   2705    3008    3114 
   Size:        303     106

  Fragments arranged by size: 

                1163     578     374     303     177     129     127     106
                 102      55
 
BsmI GAATG_Cn'

Cuts at:      0     896    1452    3114 
   Size:        896     556    1662

  Fragments arranged by size: 

                1662     896     556
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2151    2758    3114 
   Size:       2151     607     356

  Fragments arranged by size: 

                2151     607     356
 
Bsp1286I G_dGCh'C

Cuts at:      0     578     692     755     882     984    1311    1358    1463 
   Size:        578     114      63     127     102     327      47     105

Cuts at:   1463    1487    1508    1542    1688    2122    2345    2384    2705 
   Size:         24      21      34     146     434     223      39     321

Cuts at:   2705    3008    3114 
   Size:        303     106

  Fragments arranged by size: 

                 578     434     327     321     303     223     146     127
                 114     106     105     102      63      47      39      34
                  24      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     539    1016    1671    2759    3114 
   Size:        539     477     655    1088     355

  Fragments arranged by size: 

                1088     655     539     477     355
 
BsrBI CCG'CTC

Cuts at:      0    1132    2393    2579    2924    3114 
   Size:       1132    1261     186     345     190

  Fragments arranged by size: 

                1261    1132     345     190     186
 
BsrDI GCAATG_nn'

Cuts at:      0     272    2898    3114 
   Size:        272    2626     216

  Fragments arranged by size: 

                2626     272     216
 
BsrFI r'CCGG_y

Cuts at:      0    1020    2248    2297    3114 
   Size:       1020    1228      49     817

  Fragments arranged by size: 

                1228    1020     817      49
 
BssSI C'ACGA_G

Cuts at:      0     706    3114 
   Size:        706    2408
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     312    3114 
   Size:        312    2802
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1501    3114 
   Size:       1501    1613
 
BstYI r'GATC_y

Cuts at:      0    1431    3114 
   Size:       1431    1683
 
Bsu36I CC'TnA_GG

Cuts at:      0     418     761    1883    2114    3114 
   Size:        418     343    1122     231    1000

  Fragments arranged by size: 

                1122    1000     418     343     231
 
BtgI C'CryG_G

Cuts at:      0     296     721    1101    1182    1284    2546    3071    3114 
   Size:        296     425     380      81     102    1262     525      43

  Fragments arranged by size: 

                1262     525     425     380     296     102      81      43
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     989    1899    2502    3114 
   Size:        989     910     603     612

  Fragments arranged by size: 

                 989     910     612     603
 
BtsI GCAGTG_nn'

Cuts at:      0      22     163    1733    2054    2638    2910    3114 
   Size:         22     141    1570     321     584     272     204

  Fragments arranged by size: 

                1570     584     321     272     204     141      22
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2812    3114 
   Size:       2812     302
 
EaeI y'GGCC_r

Cuts at:      0     509     732    2090    2098    2250    2647    2963    3114 
   Size:        509     223    1358       8     152     397     316     151

  Fragments arranged by size: 

                1358     509     397     316     223     152     151       8
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1376    1586    3114 
   Size:       1376     210    1528

  Fragments arranged by size: 

                1528    1376     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1174    1903    1975    2353    2455    3114 
   Size:       1174     729      72     378     102     659

  Fragments arranged by size: 

                1174     729     659     378     102      72
 
EcoICRI GAG'CTC

Cuts at:      0     753    1485    3114 
   Size:        753     732    1629

  Fragments arranged by size: 

                1629     753     732
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     562     724     877    1021    1069    1169    1325 
   Size:        449     113     162     153     144      48     100     156

Cuts at:   1325    1361    1831    2029    2305    2323    2455    2764    3022 
   Size:         36     470     198     276      18     132     309     258

Cuts at:   3022    3114 
   Size:         92

  Fragments arranged by size: 

                 470     449     309     276     258     198     162     156
                 153     144     132     113     100      92      48      36
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      84     190     417     553    1000    1276    1320    1678 
   Size:         84     106     227     136     447     276      44     358

Cuts at:   1678    2434    2826    3114 
   Size:        756     392     288

  Fragments arranged by size: 

                 756     447     392     358     288     276     227     136
                 106      84      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198     449     546     558     584     689     711     827 
   Size:        198     251      97      12      26     105      22     116

Cuts at:    827    1005    1035    1269    1325    1505    1548    2118    2427 
   Size:        178      30     234      56     180      43     570     309

Cuts at:   2427    2831    3102    3114 
   Size:        404     271      12

  Fragments arranged by size: 

                 570     404     309     271     251     234     198     180
                 178     116     105      97      56      43      30      26
                  22      12      12
 
EcoRV GAT'ATC

Cuts at:      0    2988    3114 
   Size:       2988     126
 
HaeII r_GCGC'y

Cuts at:      0     312    1748    2201    2323    3114 
   Size:        312    1436     453     122     791

  Fragments arranged by size: 

                1436     791     453     312     122
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     471     503     535     567     616     648     666     698 
   Size:        471      32      32      32      49      32      18      32

Cuts at:    698    1273    1305    2644    2676    2724    2756    3114 
   Size:        575      32    1339      32      48      32     358

  Fragments arranged by size: 

                1339     575     471     358      49      48      32      32
                  32      32      32      32      32      32      18
 
HincII GTy'rAC

Cuts at:      0    1794    3114 
   Size:       1794    1320
 
HindIII A'AGCT_T

Cuts at:      0     331    3114 
   Size:        331    2783
 
MfeI C'AATT_G

Cuts at:      0    1567    3114 
   Size:       1567    1547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     546    2255    3114 
   Size:        546    1709     859

  Fragments arranged by size: 

                1709     859     546
 
MscI TGG'CCA

Cuts at:      0     511    2092    2965    3114 
   Size:        511    1581     873     149

  Fragments arranged by size: 

                1581     873     511     149
 
MslI CAynn'nnrTG

Cuts at:      0    2678    2903    3114 
   Size:       2678     225     211

  Fragments arranged by size: 

                2678     225     211
 
MspA1I CmG'CkG

Cuts at:      0     613    1471    1534    2535    3114 
   Size:        613     858      63    1001     579

  Fragments arranged by size: 

                1001     858     613     579      63
 
NaeI GCC'GGC

Cuts at:      0    1022    3114 
   Size:       1022    2092
 
NcoI C'CATG_G

Cuts at:      0    1101    3114 
   Size:       1101    2013
 
NgoMIV G'CCGG_C

Cuts at:      0    1020    3114 
   Size:       1020    2094
 
NheI G'CTAG_C

Cuts at:      0    2476    3114 
   Size:       2476     638
 
PasI CC'CwG_GG

Cuts at:      0      86    1123    1137    2526    3114 
   Size:         86    1037      14    1389     588

  Fragments arranged by size: 

                1389    1037     588      86      14
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     795    2088    3114 
   Size:        795    1293    1026

  Fragments arranged by size: 

                1293    1026     795
 
PfoI T'CCnGG_A

Cuts at:      0     539    2837    3114 
   Size:        539    2298     277

  Fragments arranged by size: 

                2298     539     277
 
PmlI CAC'GTG

Cuts at:      0     353    3114 
   Size:        353    2761
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2680    2712    3114 
   Size:       2680      32     402

  Fragments arranged by size: 

                2680     402      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     584     711    1005    2427    2831    3114 
   Size:        584     127     294    1422     404     283

  Fragments arranged by size: 

                1422     584     404     294     283     127
 
PspOMI G'GGCC_C

Cuts at:      0     688    1504    2118    3114 
   Size:        688     816     614     996

  Fragments arranged by size: 

                 996     816     688     614
 
PspXI vC'TCGA_Gb

Cuts at:      0     190    1112    3114 
   Size:        190     922    2002

  Fragments arranged by size: 

                2002     922     190
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2497    2529    3114 
   Size:       2497      32     585

  Fragments arranged by size: 

                2497     585      32
 
PstI C_TGCA'G

Cuts at:      0     308    1013    1280    1682    2756    3114 
   Size:        308     705     267     402    1074     358

  Fragments arranged by size: 

                1074     705     402     358     308     267
 
SacI G_AGCT'C

Cuts at:      0     755    1487    3114 
   Size:        755     732    1627

  Fragments arranged by size: 

                1627     755     732
 
SbfI CC_TGCA'GG

Cuts at:      0    1280    1682    3114 
   Size:       1280     402    1432

  Fragments arranged by size: 

                1432    1280     402
 
SexAI A'CCwGG_T

Cuts at:      0    2690    3114 
   Size:       2690     424
 
SfcI C'TryA_G

Cuts at:      0     304    1009    1276    1678    2752    2868    3114 
   Size:        304     705     267     402    1074     116     246

  Fragments arranged by size: 

                1074     705     402     304     267     246     116
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1043    3114 
   Size:       1043    2071
 
SmaI CCC'GGG

Cuts at:      0     687     843    1464    2425    3114 
   Size:        687     156     621     961     689

  Fragments arranged by size: 

                 961     689     687     621     156
 
SmlI C'TyrA_G

Cuts at:      0     190     885    1112    1867    2826    3009    3097    3114 
   Size:        190     695     227     755     959     183      88      17

  Fragments arranged by size: 

                 959     755     695     227     190     183      88      17
            
 
SspI AAT'ATT

Cuts at:      0      19    3114 
   Size:         19    3095
 
StuI AGG'CCT

Cuts at:      0     220     900    3114 
   Size:        220     680    2214

  Fragments arranged by size: 

                2214     680     220
 
StyI C'CwwG_G

Cuts at:      0    1101    1169    3114 
   Size:       1101      68    1945

  Fragments arranged by size: 

                1945    1101      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2123    2539    2822    3114 
   Size:       2123     416     283     292

  Fragments arranged by size: 

                2123     416     292     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     136     168    1712    1744    2296    2308    2328    2340 
   Size:        136      32    1544      32     552      12      20      12

Cuts at:   2340    3114 
   Size:        774

  Fragments arranged by size: 

                1544     774     552     136      32      32      20      12
                  12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     903    1434    1773    3114 
   Size:        903     531     339    1341

  Fragments arranged by size: 

                1341     903     531     339
 
XhoI C'TCGA_G

Cuts at:      0     190    1112    3114 
   Size:        190     922    2002

  Fragments arranged by size: 

                2002     922     190
 
XmaI C'CCGG_G

Cuts at:      0     685     841    1462    2423    3114 
   Size:        685     156     621     961     691

  Fragments arranged by size: 

                 961     691     685     621     156
 
XmnI GAAnn'nnTTC

Cuts at:      0      61    3114 
   Size:         61    3053
 
ZraI GAC'GTC

Cuts at:      0    1665    3114 
   Size:       1665    1449
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AhdI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI
     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NheI     PasI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PsrI     PstI     SacI     SbfI    SexAI
     SfcI     SfiI     SmaI     SmlI     SspI     StuI     StyI    TaqII
     TstI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AleI     AlfI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI     BclI
    BfrBI    BsiEI    BsiWI    BspEI    BspHI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI    EcoRI
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     NarI     NdeI     NotI     NruI     NsiI     NspI     PacI     PciI
     PmeI    PshAI     PsiI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SwaI     TatI  Tth111I     XbaI
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