Restriction map for pF1KSDA0383
 (Linear) (Six-base) MAPSORT of: KIAA0383.seq  Check: 5699  from: 1  to: 5343



KIAA0383.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2238    5343 
   Size:       2238    3105
 
AccI GT'mk_AC

Cuts at:      0    3560    4112    5343 
   Size:       3560     552    1231

  Fragments arranged by size: 

                3560    1231     552
 
Acc65I G'GTAC_C

Cuts at:      0    1277    5343 
   Size:       1277    4066
 
AclI AA'CG_TT

Cuts at:      0    1570    5343 
   Size:       1570    3773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     397    1211    2138    2993    3632    4551    5343 
   Size:        104     293     814     927     855     639     919     792

  Fragments arranged by size: 

                 927     919     855     814     792     639     293     104
            
 
AflII C'TTAA_G

Cuts at:      0     783    5343 
   Size:        783    4560
 
AflIII A'CryG_T

Cuts at:      0    3109    5343 
   Size:       3109    2234
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2246    4342    5003    5343 
   Size:       2246    2096     661     340

  Fragments arranged by size: 

                2246    2096     661     340
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2180    2212    4219    4251    5343 
   Size:       2180      32    2007      32    1092

  Fragments arranged by size: 

                2180    2007    1092      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     453     821    2116    2307    4205    4419    4930 
   Size:        142     311     368    1295     191    1898     214     511

Cuts at:   4930    5343 
   Size:        413

  Fragments arranged by size: 

                1898    1295     511     413     368     311     214     191
                 142
 
ApoI r'AATT_y

Cuts at:      0     748    1294    1378    1463    1517    2937    5343 
   Size:        748     546      84      85      54    1420    2406

  Fragments arranged by size: 

                2406    1420     748     546      85      84      54
 
AvaI C'yCGr_G

Cuts at:      0    4477    5343 
   Size:       4477     866
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     541     574    5343 
   Size:        541      33    4769

  Fragments arranged by size: 

                4769     541      33
 
BamHI G'GATC_C

Cuts at:      0    1081    5343 
   Size:       1081    4262
 
BanI G'GyrC_C

Cuts at:      0     549    1277    5209    5343 
   Size:        549     728    3932     134

  Fragments arranged by size: 

                3932     728     549     134
 
BanII G_rGCy'C

Cuts at:      0    2600    3590    5343 
   Size:       2600     990    1753

  Fragments arranged by size: 

                2600    1753     990
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139    1271    2926    3329    5343 
   Size:        139    1132    1655     403    2014

  Fragments arranged by size: 

                2014    1655    1132     403     139
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3672    3706    5343 
   Size:       3672      34    1637

  Fragments arranged by size: 

                3672    1637      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     569    1639    5343 
   Size:        569    1070    3704

  Fragments arranged by size: 

                3704    1070     569
 
BclI T'GATC_A

Cuts at:      0    1700    3342    4608    5343 
   Size:       1700    1642    1266     735

  Fragments arranged by size: 

                1700    1642    1266     735
 
BfrBI ATG'CAT

Cuts at:      0    1093    5343 
   Size:       1093    4250
 
BlpI GC'TnA_GC

Cuts at:      0    2147    5343 
   Size:       2147    3196
 
Bme1580I G_kGCm'C

Cuts at:      0     552    1927    3117    5212    5343 
   Size:        552    1375    1190    2095     131

  Fragments arranged by size: 

                2095    1375    1190     552     131
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     469     557    3023    3074    3913    5343 
   Size:        469      88    2466      51     839    1430

  Fragments arranged by size: 

                2466    1430     839     469      88      51
 
BmtI G_CTAG'C

Cuts at:      0    2171    5343 
   Size:       2171    3172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1436    2329    2659    4239    5343 
   Size:       1436     893     330    1580    1104

  Fragments arranged by size: 

                1580    1436    1104     893     330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    3068    3803    4361    5343 
   Size:        373    2695     735     558     982

  Fragments arranged by size: 

                2695     982     735     558     373
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1793    2557    5343 
   Size:       1793     764    2786

  Fragments arranged by size: 

                2786    1793     764
 
BsaHI Gr'CG_yC

Cuts at:      0    3557    5343 
   Size:       3557    1786
 
BsaWI w'CCGG_w

Cuts at:      0    2923    5343 
   Size:       2923    2420
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3239    3269    4219    4237    4249    4267    4767    4797 
   Size:       3239      30     950      18      12      18     500      30

Cuts at:   4797    5343 
   Size:        546

  Fragments arranged by size: 

                3239     950     546     500      30      30      18      18
                  12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     360     370     691    1317    2356    2359    2362    2371 
   Size:        360      10     321     626    1039       3       3       9

Cuts at:   2371    2374    2386    2413    3061    3064    3202    3205    3208 
   Size:          3      12      27     648       3     138       3       3

Cuts at:   3208    3211    3260    3367    3370    3649    4364    4769    4772 
   Size:          3      49     107       3     279     715     405       3

Cuts at:   4772    5343 
   Size:        571

  Fragments arranged by size: 

                1039     715     648     626     571     405     360     321
                 279     138     107      49      27      12      10       9
                   3       3       3       3       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     435    1709    2174    3601    3717    4289    4537    5117 
   Size:        435    1274     465    1427     116     572     248     580

Cuts at:   5117    5213    5225    5343 
   Size:         96      12     118

  Fragments arranged by size: 

                1427    1274     580     572     465     435     248     118
                 116      96      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731     977    1172    3314    3535    3875    4449    4973 
   Size:        731     246     195    2142     221     340     574     524

Cuts at:   4973    5343 
   Size:        370

  Fragments arranged by size: 

                2142     731     574     524     370     340     246     221
                 195
 
BsiEI CG_ry'CG

Cuts at:      0    1033    5343 
   Size:       1033    4310
 
BsiHKAI G_wGCw'C

Cuts at:      0    4150    5343 
   Size:       4150    1193
 
BsiWI C'GTAC_G

Cuts at:      0    3954    5343 
   Size:       3954    1389
 
BsmI GAATG_Cn'

Cuts at:      0     568     808     898     923    1093    1095    2262    2698 
   Size:        568     240      90      25     170       2    1167     436

Cuts at:   2698    2775    5343 
   Size:         77    2568

  Fragments arranged by size: 

                2568    1167     568     436     240     170      90      77
                  25       2
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1825    2569    4474    4919    5343 
   Size:       1825     744    1905     445     424

  Fragments arranged by size: 

                1905    1825     744     445     424
 
Bsp1286I G_dGCh'C

Cuts at:      0     552    1927    2600    3117    3590    4150    5212    5343 
   Size:        552    1375     673     517     473     560    1062     131

  Fragments arranged by size: 

                1375    1062     673     560     552     517     473     131
            
 
BspHI T'CATG_A

Cuts at:      0     981    5343 
   Size:        981    4362
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2238    2970    4366    5343 
   Size:       2238     732    1396     977

  Fragments arranged by size: 

                2238    1396     977     732
 
BsrBI CCG'CTC

Cuts at:      0    2151    5343 
   Size:       2151    3192
 
BsrDI GCAATG_nn'

Cuts at:      0     315    1934    2757    4615    5343 
   Size:        315    1619     823    1858     728

  Fragments arranged by size: 

                1858    1619     823     728     315
 
BsrFI r'CCGG_y

Cuts at:      0    1274    2276    5343 
   Size:       1274    1002    3067

  Fragments arranged by size: 

                3067    1274    1002
 
BsrGI T'GTAC_A

Cuts at:      0    2942    5264    5343 
   Size:       2942    2322      79

  Fragments arranged by size: 

                2942    2322      79
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     453     803     812    4660    5235    5343 
   Size:        453     350       9    3848     575     108

  Fragments arranged by size: 

                3848     575     453     350     108       9
 
BstEII G'GTnAC_C

Cuts at:      0    1109    5343 
   Size:       1109    4234
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4464    5343 
   Size:       4464     879
 
BstYI r'GATC_y

Cuts at:      0    1081    3264    5343 
   Size:       1081    2183    2079

  Fragments arranged by size: 

                2183    2079    1081
 
Bsu36I CC'TnA_GG

Cuts at:      0    3173    5343 
   Size:       3173    2170
 
BtgI C'CryG_G

Cuts at:      0     117    2963    3311    3725    5250    5343 
   Size:        117    2846     348     414    1525      93

  Fragments arranged by size: 

                2846    1525     414     348     117      93
 
BtsI GCAGTG_nn'

Cuts at:      0     799     822    2117    2334    2556    5343 
   Size:        799      23    1295     217     222    2787

  Fragments arranged by size: 

                2787    1295     799     222     217      23
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1416    1451    5343 
   Size:       1416      35    3892

  Fragments arranged by size: 

                3892    1416      35
 
DraI TTT'AAA

Cuts at:      0    2931    5343 
   Size:       2931    2412
 
DraIII CAC_nnn'GTG

Cuts at:      0    3142    5343 
   Size:       3142    2201
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3556    5343 
   Size:       3556    1787
 
EaeI y'GGCC_r

Cuts at:      0    1272    2278    2866    3945    5343 
   Size:       1272    1006     588    1079    1398

  Fragments arranged by size: 

                1398    1272    1079    1006     588
 
EarI CTCTTCn'nnn_

Cuts at:      0      80    1187    1558    1764    1837    2117    2333    2363 
   Size:         80    1107     371     206      73     280     216      30

Cuts at:   2363    2375    2381    2390    2402    2612    2732    3173    3179 
   Size:         12       6       9      12     210     120     441       6

Cuts at:   3179    3197    3206    3215    3344    3356    3365    3443    4224 
   Size:         18       9       9     129      12       9      78     781

Cuts at:   4224    5343 
   Size:       1119

  Fragments arranged by size: 

                1119    1107     781     441     371     280     216     210
                 206     129     120      80      78      73      30      18
                  12      12      12       9       9       9       9       6
                   6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     397    1211    1436    2138    2329    2659    2993 
   Size:        104     293     814     225     702     191     330     334

Cuts at:   2993    3632    4239    4551    5343 
   Size:        639     607     312     792

  Fragments arranged by size: 

                 814     792     702     639     607     334     330     312
                 293     225     191     104
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1654    5343 
   Size:       1654    3689
 
EcoO109I rG'GnC_Cy

Cuts at:      0      77     216    3606    4735    4984    5343 
   Size:         77     139    3390    1129     249     359

  Fragments arranged by size: 

                3390    1129     359     249     139      77
 
EcoRI G'AATT_C

Cuts at:      0    1378    5343 
   Size:       1378    3965
 
HaeII r_GCGC'y

Cuts at:      0     237    4962    5047    5343 
   Size:        237    4725      85     296

  Fragments arranged by size: 

                4725     296     237      85
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1066    1098    1724    1756    1976    2008    4066    4098 
   Size:       1066      32     626      32     220      32    2058      32

Cuts at:   4098    4187    4219    4767    4799    5343 
   Size:         89      32     548      32     544

  Fragments arranged by size: 

                2058    1066     626     548     544     220      89      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2204    3561    3966    4592    5061    5343 
   Size:       2204    1357     405     626     469     282

  Fragments arranged by size: 

                2204    1357     626     469     405     282
 
HindIII A'AGCT_T

Cuts at:      0    1363    1663    3465    5343 
   Size:       1363     300    1802    1878

  Fragments arranged by size: 

                1878    1802    1363     300
 
HpaI GTT'AAC

Cuts at:      0    4592    5343 
   Size:       4592     751
 
KpnI G_GTAC'C

Cuts at:      0    1281    5343 
   Size:       1281    4062
 
MfeI C'AATT_G

Cuts at:      0     341    5343 
   Size:        341    5002
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     104    1623    3884    4325    4378    5343 
   Size:        104    1519    2261     441      53     965

  Fragments arranged by size: 

                2261    1519     965     441     104      53
 
MscI TGG'CCA

Cuts at:      0    2868    3947    5343 
   Size:       2868    1079    1396

  Fragments arranged by size: 

                2868    1396    1079
 
MslI CAynn'nnrTG

Cuts at:      0    1874    4033    4894    5020    5026    5077    5302    5343 
   Size:       1874    2159     861     126       6      51     225      41

  Fragments arranged by size: 

                2159    1874     861     225     126      51      41       6
            
 
MspA1I CmG'CkG

Cuts at:      0     150     450    2304    2637    4232    4277    4310    4419 
   Size:        150     300    1854     333    1595      45      33     109

Cuts at:   4419    4464    4636    4930    5343 
   Size:         45     172     294     413

  Fragments arranged by size: 

                1854    1595     413     333     300     294     172     150
                 109      45      45      33
 
NaeI GCC'GGC

Cuts at:      0    2278    5343 
   Size:       2278    3065
 
NcoI C'CATG_G

Cuts at:      0     117    2963    3725    5343 
   Size:        117    2846     762    1618

  Fragments arranged by size: 

                2846    1618     762     117
 
NdeI CA'TA_TG

Cuts at:      0     887    5220    5343 
   Size:        887    4333     123

  Fragments arranged by size: 

                4333     887     123
 
NgoMIV G'CCGG_C

Cuts at:      0    2276    5343 
   Size:       2276    3067
 
NheI G'CTAG_C

Cuts at:      0    2167    5343 
   Size:       2167    3176
 
NsiI A_TGCA'T

Cuts at:      0    1095    5343 
   Size:       1095    4248
 
NspI r_CATG'y

Cuts at:      0     815    1923    3113    4326    5115    5307    5343 
   Size:        815    1108    1190    1213     789     192      36

  Fragments arranged by size: 

                1213    1190    1108     815     789     192      36
 
PasI CC'CwG_GG

Cuts at:      0     229    4729    5343 
   Size:        229    4500     614

  Fragments arranged by size: 

                4500     614     229
 
PciI A'CATG_T

Cuts at:      0    3109    5343 
   Size:       3109    2234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     123    4419    5343 
   Size:        123    4296     924

  Fragments arranged by size: 

                4296     924     123
 
PfoI T'CCnGG_A

Cuts at:      0    1556    5343 
   Size:       1556    3787
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4219    4251    5343 
   Size:       4219      32    1092

  Fragments arranged by size: 

                4219    1092      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     216    3606    4735    4984    5343 
   Size:        216    3390    1129     249     359

  Fragments arranged by size: 

                3390    1129     359     249     216
 
PshAI GACnn'nnGTC

Cuts at:      0    3863    5343 
   Size:       3863    1480
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5122    5154    5343 
   Size:       5122      32     189

  Fragments arranged by size: 

                5122     189      32
 
PstI C_TGCA'G

Cuts at:      0     952    1952    2112    4049    4321    4331    4363    4586 
   Size:        952    1000     160    1937     272      10      32     223

Cuts at:   4586    5343 
   Size:        757

  Fragments arranged by size: 

                1937    1000     952     757     272     223     160      32
                  10
 
PvuII CAG'CTG

Cuts at:      0     150    2304    2637    4232    4277    4419    4464    5343 
   Size:        150    2154     333    1595      45     142      45     879

  Fragments arranged by size: 

                2154    1595     879     333     150     142      45      45
            
 
RsrII CG'GwC_CG

Cuts at:      0     505    5343 
   Size:        505    4838
 
SalI G'TCGA_C

Cuts at:      0    3559    5343 
   Size:       3559    1784
 
SanDI GG'GwC_CC

Cuts at:      0    3606    4735    5343 
   Size:       3606    1129     608

  Fragments arranged by size: 

                3606    1129     608
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1558    1837    2117    3365    3443    4224    5343 
   Size:       1558     279     280    1248      78     781    1119

  Fragments arranged by size: 

                1558    1248    1119     781     280     279      78
 
ScaI AGT'ACT

Cuts at:      0     111    2588    3479    5343 
   Size:        111    2477     891    1864

  Fragments arranged by size: 

                2477    1864     891     111
 
SexAI A'CCwGG_T

Cuts at:      0    1318    3138    5343 
   Size:       1318    1820    2205

  Fragments arranged by size: 

                2205    1820    1318
 
SfcI C'TryA_G

Cuts at:      0     822     948    1948    2108    3828    3838    4045    4239 
   Size:        822     126    1000     160    1720      10     207     194

Cuts at:   4239    4317    4327    4359    4582    4970    5343 
   Size:         78      10      32     223     388     373

  Fragments arranged by size: 

                1720    1000     822     388     373     223     207     194
                 160     126      78      32      10      10
 
SmlI C'TyrA_G

Cuts at:      0     352     783    3083    3818    4376    5343 
   Size:        352     431    2300     735     558     967

  Fragments arranged by size: 

                2300     967     735     558     431     352
 
SphI G_CATG'C

Cuts at:      0    4326    5343 
   Size:       4326    1017
 
SspI AAT'ATT

Cuts at:      0    1410    2257    2439    4836    5343 
   Size:       1410     847     182    2397     507

  Fragments arranged by size: 

                2397    1410     847     507     182
 
StuI AGG'CCT

Cuts at:      0     200     781    2499    3826    4398    5343 
   Size:        200     581    1718    1327     572     945

  Fragments arranged by size: 

                1718    1327     945     581     572     200
 
StyI C'CwwG_G

Cuts at:      0     117     275     831    2963    3590    3725    5343 
   Size:        117     158     556    2132     627     135    1618

  Fragments arranged by size: 

                2132    1618     627     556     158     135     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     749    1047    2976    4303    4470    4951    5227    5343 
   Size:        749     298    1929    1327     167     481     276     116

  Fragments arranged by size: 

                1929    1327     749     481     298     276     167     116
            
 
TatI w'GTAC_w

Cuts at:      0     109     512    2238    2505    2586    2942    3477    5264 
   Size:        109     403    1726     267      81     356     535    1787

Cuts at:   5264    5343 
   Size:         79

  Fragments arranged by size: 

                1787    1726     535     403     356     267     109      81
                  79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4709    4741    5343 
   Size:       4709      32     602

  Fragments arranged by size: 

                4709     602      32
 
XbaI T'CTAG_A

Cuts at:      0     283    1765    5343 
   Size:        283    1482    3578

  Fragments arranged by size: 

                3578    1482     283
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     929    1554    2710    3940    4068    5149    5343 
   Size:        929     625    1156    1230     128    1081     194

  Fragments arranged by size: 

                1230    1156    1081     929     625     194     128
 
XmnI GAAnn'nnTTC

Cuts at:      0    1093    2438    5343 
   Size:       1093    1345    2905

  Fragments arranged by size: 

                2905    1345    1093
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AlfI
     AloI    AlwNI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI    PshAI
     PsrI     PstI    PvuII    RsrII     SalI    SanDI     SapI     ScaI
    SexAI     SfcI     SmlI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AgeI     AhdI     AleI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BbeI    BbvCI     BglI    BglII    BmgBI
     BplI   Bpu10I    BsaAI    BsaBI    BspEI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI     ClaI     EagI     EciI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI     KasI     MluI     NarI     NotI     NruI
     PacI     PmeI     PmlI     PsiI   PspOMI    PspXI     PvuI     SacI
    SacII     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SwaI  Tth111I     XhoI     XmaI     ZraI
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