Restriction map for pF1KSDA0332
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflIII      
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AvaI        AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BglI        BlnI        
BpmI        BsaHI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       BspHI       BspLU11I    BspMI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
Cfr10I      CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       FseI        HaeII       Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MfeI        MluI        MluNI       MscI        
MunI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tsp45I      
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         2822  GTAGAC               GT^[AC][GT]AC
AccII         738  CGCG                 CG^CG
AccIII       1135  TCCGGA               T^CCGGA
AflII        1600  CTTAAG               C^TTAAG
Alw44I       2114  GTGCAC               G^TGCAC
ApaLI        2114  GTGCAC               G^TGCAC
AseI          841  ATTAAT               AT^TAAT
AsnI          841  ATTAAT               AT^TAAT
Asp700I      1474  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1152  TGCGCA               TGC^GCA
BanI         2086  GGTGCC               G^G[CT][GA]CC
BclI         2003  TGATCA               T^GATCA
BfrI         1600  CTTAAG               C^TTAAG
BlpI          274  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      274  GCTAAGC              GC^T[ACGT]AGC
BsaAI        1316  TACGTG               [CT]AC^GT[GA]
BsaI         3056  GGTCTC               GGTCTC(1/5)
BsaWI        1135  TCCGGA               [AT]^CCGG[AT]
BseAI        1135  TCCGGA               T^CCGGA
BsmFI        2179  GTCCC                GGGAC(10/14)
BsmI          247  GCATTC               GAATGC(1/-1)
Bsp1407I     1805  TGTACA               T^GTACA
BspDI        2161  ATCGAT               AT^CGAT
BspEI        1135  TCCGGA               T^CCGGA
BsrBI        1165  GAGCGG               CCGCTC(-3/-3)
BsrDI        1675  GCAATG               GCAATG(2/0)
BsrGI        1805  TGTACA               T^GTACA
BstUI         738  CGCG                 CG^CG
CelII         274  GCTAAGC              GC^T[ACGT]AGC
ClaI         2161  ATCGAT               AT^CGAT
EcoRI        2138  GAATTC               G^AATTC
EcoT22I        95  ATGCAT               ATGCA^T
FauI         1638  GCGGG                CCCGC(4/6)
FbaI         2003  TGATCA               T^GATCA
FspI         1152  TGCGCA               TGC^GCA
HapII        1136  CCGG                 C^CGG
HpaII        1136  CCGG                 C^CGG
MroI         1135  TCCGGA               T^CCGGA
MspA1I        335  CAGCTG               C[AC]G^C[GT]G
MspI         1136  CCGG                 C^CGG
MvnI          738  CGCG                 CG^CG
NdeI           88  CATATG               CA^TATG
NsiI           95  ATGCAT               ATGCA^T
PshBI         841  ATTAAT               AT^TAAT
PstI         1441  CTGCAG               CTGCA^G
PvuII         335  CAGCTG               CAG^CTG
ScaI         2495  AGTACT               AGT^ACT
SspBI        1805  TGTACA               T^GTACA
XbaI         2998  TCTAGA               T^CTAGA
XmnI         1474  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwNI         335  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              707  CAGATTCTG           
AspI         1230  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2475  GACTGAGTC           
BsaBI        2197  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2951  GATCACCATC          
BsaJI         831  CCTTGG               C^C[ACGT][ACGT]GG
             1651  CCAAGG              
BsiYI        1129  CCTCCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2091  CCCCATCGAGG         
BslI         1129  CCTCCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2091  CCCCATCGAGG         
Cac8I        1239  GCAAGC               GC[ACGT]^[ACGT]GC
             1281  GCTCGC              
CfoI          739  GCGC                 GCG^C
             1153  GCGC                
DraI         1002  TTTAAA               TTT^AAA
             2260  TTTAAA              
EcoT14I       831  CCTTGG               C^C[AT][AT]GG
             1651  CCAAGG              
HgaI          736  GACGC                GACGC(5/10)
              723  GCGTC               
HhaI          739  GCGC                 GCG^C
             1153  GCGC                
HinP1I        739  GCGC                 G^CGC
             1153  GCGC                
HindIII      1426  AAGCTT               A^AGCTT
             1565  AAGCTT              
HphI         2842  TCACC                GGTGA(8/7)
             2953  TCACC               
MaeII         651  ACGT                 A^CGT
             1317  ACGT                
MaeIII       1194  GTTAC                ^GT[ACGT]AC
             2320  GTTAC               
MamI         2197  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2951  GATCACCATC          
MslI           88  CATATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2273  CATCAAAATG          
PflFI        1230  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2475  GACTGAGTC           
SacI         2542  GAGCTC               GAGCT^C
             2889  GAGCTC              
SapI         1549  GAAGAGC              GCTCTTC(1/4)
             2101  GAAGAGC             
SmlI          216  CTCAAG               C^T[CT][GA]AG
             1600  CTTAAG              
SspI         2017  AATATT               AAT^ATT
             2038  AATATT              
StyI          831  CCTTGG               C^C[AT][AT]GG
             1651  CCAAGG              
TspRI         903  CAGTG                CA[GC]TG[ACGT][ACGT]^
             2817  CAGTG               
Tth111I      1230  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2475  GACTGAGTC           
XcmI         1244  CCATAGTCAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1778  CCAGATTATATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BfaI        BglII       BmyI        BpuAI       
BseRI       BsiHKAI     BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TthHB8I     
XhoII       
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