Restriction map for pF1KSDA0332
 (Linear) (Six-base) MAPSORT of: KIAA0332.seq  Check: 1563  from: 1  to: 3087



KIAA0332.orf

 With 182 enzymes: SgfI * 

                        July 23, 2007 10:03  ..

AccI GT'mk_AC

Cuts at:      0    2823    3087 
   Size:       2823     264
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1721    1730    1799    1944    2252    2453    3087 
   Size:         62    1659       9      69     145     308     201     634

  Fragments arranged by size: 

                1659     634     308     201     145      69      62       9
            
 
AflII C'TTAA_G

Cuts at:      0    1600    3087 
   Size:       1600    1487
 
AlwNI CAG_nnn'CTG

Cuts at:      0     340     712    3087 
   Size:        340     372    2375

  Fragments arranged by size: 

                2375     372     340
 
ApaLI G'TGCA_C

Cuts at:      0    2114    3087 
   Size:       2114     973
 
ApoI r'AATT_y

Cuts at:      0     638     877    1008    1275    1849    2138    3087 
   Size:        638     239     131     267     574     289     949

  Fragments arranged by size: 

                 949     638     574     289     267     239     131
 
AseI AT'TA_AT

Cuts at:      0     842    3087 
   Size:        842    2245
 
BanI G'GyrC_C

Cuts at:      0    2086    3087 
   Size:       2086    1001
 
BanII G_rGCy'C

Cuts at:      0     148    2546    2893    3087 
   Size:        148    2398     347     194

  Fragments arranged by size: 

                2398     347     194     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     512    1234    2240    3087 
   Size:        512     722    1006     847

  Fragments arranged by size: 

                1006     847     722     512
 
BclI T'GATC_A

Cuts at:      0    2003    3087 
   Size:       2003    1084
 
BfrBI ATG'CAT

Cuts at:      0      97    3087 
   Size:         97    2990
 
BglII A'GATC_T

Cuts at:      0    2875    3001    3043    3087 
   Size:       2875     126      42      44

  Fragments arranged by size: 

                2875     126      44      42
 
BlpI GC'TnA_GC

Cuts at:      0     275    3087 
   Size:        275    2812
 
Bme1580I G_kGCm'C

Cuts at:      0     180    2091    2118    3087 
   Size:        180    1911      27     969

  Fragments arranged by size: 

                1911     969     180      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3030    3087 
   Size:       3030      57
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201    3087 
   Size:        201    2886
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3062    3087 
   Size:       3062      25
 
BsaAI yAC'GTr

Cuts at:      0    1318    3087 
   Size:       1318    1769
 
BsaBI GATnn'nnATC

Cuts at:      0    2201    2955    3087 
   Size:       2201     754     132

  Fragments arranged by size: 

                2201     754     132
 
BsaWI w'CCGG_w

Cuts at:      0    1135    3087 
   Size:       1135    1952
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     191     367     689    3087 
   Size:        191     176     322    2398

  Fragments arranged by size: 

                2398     322     191     176
 
BseYI C'CCAG_C

Cuts at:      0    2797    3087 
   Size:       2797     290
 
BsiHKAI G_wGCw'C

Cuts at:      0    1599    2118    2546    2893    3087 
   Size:       1599     519     428     347     194

  Fragments arranged by size: 

                1599     519     428     347     194
 
BsmI GAATG_Cn'

Cuts at:      0     247    3087 
   Size:        247    2840
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     180    1599    2091    2118    2546    2893    3087 
   Size:        148      32    1419     492      27     428     347     194

  Fragments arranged by size: 

                1419     492     428     347     194     148      32      27
            
 
BspEI T'CCGG_A

Cuts at:      0    1135    3087 
   Size:       1135    1952
 
BsrBI CCG'CTC

Cuts at:      0    1167    3087 
   Size:       1167    1920
 
BsrDI GCAATG_nn'

Cuts at:      0    1682    3087 
   Size:       1682    1405
 
BsrGI T'GTAC_A

Cuts at:      0    1805    3087 
   Size:       1805    1282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     880    1463    2279    3087 
   Size:        880     583     816     808

  Fragments arranged by size: 

                 880     816     808     583
 
BstYI r'GATC_y

Cuts at:      0      22      73     807    1498    2875    3001    3031    3043 
   Size:         22      51     734     691    1377     126      30      12

Cuts at:   3043    3087 
   Size:         44

  Fragments arranged by size: 

                1377     734     691     126      51      44      30      22
                  12
 
BtsI GCAGTG_nn'

Cuts at:      0    2823    3087 
   Size:       2823     264
 
ClaI AT'CG_AT

Cuts at:      0    2162    3087 
   Size:       2162     925
 
DraI TTT'AAA

Cuts at:      0    1004    2262    3087 
   Size:       1004    1258     825

  Fragments arranged by size: 

                1258    1004     825
 
EarI CTCTTCn'nnn_

Cuts at:      0     230     548     622     978    1544    1818    2096    2770 
   Size:        230     318      74     356     566     274     278     674

Cuts at:   2770    3087 
   Size:        317

  Fragments arranged by size: 

                 674     566     356     318     317     278     274     230
                  74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19      35    1085    1482    3087 
   Size:         19      16    1050     397    1605

  Fragments arranged by size: 

                1605    1050     397      19      16
 
EcoICRI GAG'CTC

Cuts at:      0    2544    2891    3087 
   Size:       2544     347     196

  Fragments arranged by size: 

                2544     347     196
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1721    1730    1799    1944    2252    2453    3087 
   Size:         62    1659       9      69     145     308     201     634

  Fragments arranged by size: 

                1659     634     308     201     145      69      62       9
            
 
EcoRI G'AATT_C

Cuts at:      0    2138    3087 
   Size:       2138     949
 
FspI TGC'GCA

Cuts at:      0    1154    3087 
   Size:       1154    1933
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     452     484    1726    1758    3087 
   Size:        452      32    1242      32    1329

  Fragments arranged by size: 

                1329    1242     452      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1426    1565    3087 
   Size:       1426     139    1522

  Fragments arranged by size: 

                1522    1426     139
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     109    1480    2782    3087 
   Size:        109    1371    1302     305

  Fragments arranged by size: 

                1371    1302     305     109
 
MslI CAynn'nnrTG

Cuts at:      0      92    2277    3087 
   Size:         92    2185     810

  Fragments arranged by size: 

                2185     810      92
 
MspA1I CmG'CkG

Cuts at:      0     337    3087 
   Size:        337    2750
 
NdeI CA'TA_TG

Cuts at:      0      89    3087 
   Size:         89    2998
 
NsiI A_TGCA'T

Cuts at:      0      99    3087 
   Size:         99    2988
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2470    2502    3087 
   Size:       2470      32     585

  Fragments arranged by size: 

                2470     585      32
 
PstI C_TGCA'G

Cuts at:      0    1445    3087 
   Size:       1445    1642
 
PvuII CAG'CTG

Cuts at:      0     337    3087 
   Size:        337    2750
 
SacI G_AGCT'C

Cuts at:      0    2546    2893    3087 
   Size:       2546     347     194

  Fragments arranged by size: 

                2546     347     194
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1544    2096    3087 
   Size:       1544     552     991

  Fragments arranged by size: 

                1544     991     552
 
ScaI AGT'ACT

Cuts at:      0    2497    3087 
   Size:       2497     590
 
SfcI C'TryA_G

Cuts at:      0    1416    1441    2631    3087 
   Size:       1416      25    1190     456

  Fragments arranged by size: 

                1416    1190     456      25
 
SmlI C'TyrA_G

Cuts at:      0     216    1600    3087 
   Size:        216    1384    1487

  Fragments arranged by size: 

                1487    1384     216
 
SspI AAT'ATT

Cuts at:      0    2019    2040    3087 
   Size:       2019      21    1047

  Fragments arranged by size: 

                2019    1047      21
 
StyI C'CwwG_G

Cuts at:      0     831    1651    3087 
   Size:        831     820    1436

  Fragments arranged by size: 

                1436     831     820
 
TatI w'GTAC_w

Cuts at:      0     268    1805    2447    2495    2752    2788    3087 
   Size:        268    1537     642      48     257      36     299

  Fragments arranged by size: 

                1537     642     299     268     257      48      36
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     159     191    2776    2808    3087 
   Size:        159      32    2585      32     279

  Fragments arranged by size: 

                2585     279     159      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1233    2478    3087 
   Size:       1233    1245     609

  Fragments arranged by size: 

                1245    1233     609
 
XbaI T'CTAG_A

Cuts at:      0    2998    3087 
   Size:       2998      89
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1251    1785    3087 
   Size:       1251     534    1302

  Fragments arranged by size: 

                1302    1251     534
 
XmnI GAAnn'nnTTC

Cuts at:      0    1478    3087 
   Size:       1478    1609
 

 Enzymes that do cut:

     AccI     AcuI    AflII    AlwNI    ApaLI     ApoI     AseI     BanI
    BanII     BbsI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspEI    BsrBI    BsrDI    BsrGI    BstXI    BstYI
     BtsI     ClaI     DraI     EarI     EciI  EcoICRI  Eco57MI    EcoRI
     FspI    Hin4I  HindIII     MmeI     MslI   MspA1I     NdeI     NsiI
     PpiI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmlI
     SspI     StyI     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BglI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BsaHI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspHI    BspMI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI   DraIII
     DrdI     EaeI     EagI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     XhoI     XmaI     ZraI
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