Restriction map for pF1KSDA0331
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BsaAI       BsgI        
BsiEI       BsiWI       BspDI       BspHI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
CfoI        Cfr10I      ClaI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HincII      HindII      
HpaI        KasI        KpnI        MfeI        MluI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        1345  GACGTC               GACGT^C
AccII           9  CGCG                 CG^CG
AccIII       1673  TCCGGA               T^CCGGA
AcyI         1345  GACGTC               G[GA]^CG[CT]C
AhdI           60  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2199  GTGCAC               G^TGCAC
AlwNI        1940  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        2199  GTGCAC               G^TGCAC
AspEI          60  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1595  CTCGAG               C^[CT]CG[GA]G
BalI         1335  TGGCCA               TGG^CCA
BanII         115  GAGCTC               G[GA]GC[CT]^C
BglII         484  AGATCT               A^GATCT
BlpI         1705  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1705  GCTCAGC              GC^T[ACGT]AGC
BsaBI         190  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1345  GACGTC               G[GA]^CG[CT]C
BsaI         1598  GAGACC               GGTCTC(1/5)
BsaWI        1673  TCCGGA               [AT]^CCGG[AT]
BseAI        1673  TCCGGA               T^CCGGA
BsmBI         593  GAGACG               CGTCTC(1/5)
BsoBI        1595  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1842  TGTACA               T^GTACA
BspEI        1673  TCCGGA               T^CCGGA
BspMI        1248  ACCTGC               ACCTGC(4/8)
BsrGI        1842  TGTACA               T^GTACA
BstAPI       1622  GCATATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI           9  CGCG                 CG^CG
CelII        1705  GCTCAGC              GC^T[ACGT]AGC
CpoI         1991  CGGTCCG              CG^G[AT]CCG
Eam1105I       60  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2134  GAATTC               G^AATTC
EcoRV         443  GATATC               GAT^ATC
EcoT22I       312  ATGCAT               ATGCA^T
Hin1I        1345  GACGTC               G[GA]^CG[CT]C
HindIII      2221  AAGCTT               A^AGCTT
KspI            8  CCGCGG               CCGC^GG
MamI          190  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1335  TGGCCA               TGG^CCA
MroI         1673  TCCGGA               T^CCGGA
MscI         1335  TGGCCA               TGG^CCA
MvnI            9  CGCG                 CG^CG
NcoI         2122  CCATGG               C^CATGG
NdeI         1067  CATATG               CA^TATG
NsiI          312  ATGCAT               ATGCA^T
PaeR7I       1595  CTCGAG               C^TCGAG
RsrII        1991  CGGTCCG              CG^G[AT]CCG
SacI          115  GAGCTC               GAGCT^C
SacII           8  CCGCGG               CCGC^GG
SapI          207  GCTCTTC              GCTCTTC(1/4)
ScaI         1789  AGTACT               AGT^ACT
SfcI          573  CTACAG               C^T[GA][CT]AG
SphI         1014  GCATGC               GCATG^C
SspBI        1842  TGTACA               T^GTACA
StuI         1132  AGGCCT               AGG^CCT
XbaI         1434  TCTAGA               T^CTAGA
XcmI         2306  CCAGGCACACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1595  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          676  AAATTT               [GA]^AATT[CT]
             2134  GAATTC              
AflIII       1034  ACATGT               A^C[GA][CT]GT
             1559  ACATGT              
AocI         1923  CCTAAGG              CC^T[ACGT]AGG
             2260  CCTCAGG             
ApoI          676  AAATTT               [GA]^AATT[CT]
             2134  GAATTC              
Asp700I      1426  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1438  GAAGAACTTC          
BamHI         456  GGATCC               G^GATCC
             1458  GGATCC              
BbsI          709  GAAGAC               GAAGAC(2/6)
             1878  GAAGAC              
BbvCI         240  CCTCAGC              CCTCAGC(-5/-2)
             1327  GCTGAGG             
BcnI           92  CCCGG                CC^[GC]GG
             1634  CCCGG               
BfaI         1040  CTAG                 C^TAG
             1435  CTAG                
BpuAI         709  GAAGAC               GAAGAC(2/6)
             1878  GAAGAC              
BsmI          310  GAATGC               GAATGC(1/-1)
             1043  GCATTC              
BspLU11I     1034  ACATGT               A^CATGT
             1559  ACATGT              
Bsu36I       1923  CCTAAGG              CC^T[ACGT]AGG
             2260  CCTCAGG             
DsaI            8  CCGCGG               C^C[GA][CT]GG
             2122  CCATGG              
EaeI          283  TGGCCG               [CT]^GGCC[GA]
             1335  TGGCCA              
Eco81I       1923  CCTAAGG              CC^T[ACGT]AGG
             2260  CCTCAGG             
FauI           10  GCGGG                CCCGC(4/6)
              334  GCGGG               
HgaI          595  GACGC                GACGC(5/10)
              657  GCGTC               
MaeI         1040  CTAG                 C^TAG
             1435  CTAG                
MwoI          754  GCACTGGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1622  GCATATCCTGC         
NciI           92  CCCGG                CC^[GC]GG
             1634  CCCGG               
SmlI         1535  CTCAAG               C^T[CT][GA]AG
             1595  CTCGAG              
XmnI         1426  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1438  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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