Restriction map for pF1KSDA0287
 (Linear) (Six-base) MAPSORT of: KIAA0287.seq  Check: 2350  from: 1  to: 4392



KIAA0287.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AatII G_ACGT'C

Cuts at:      0    4331    4392 
   Size:       4331      61
 
AccI GT'mk_AC

Cuts at:      0    1931    2041    4392 
   Size:       1931     110    2351

  Fragments arranged by size: 

                2351    1931     110
 
AclI AA'CG_TT

Cuts at:      0    1137    4392 
   Size:       1137    3255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     527    1270    2123    2718    3914    4043    4064 
   Size:        431      96     743     853     595    1196     129      21

Cuts at:   4064    4392 
   Size:        328

  Fragments arranged by size: 

                1196     853     743     595     431     328     129      96
                  21
 
AgeI A'CCGG_T

Cuts at:      0     635    4392 
   Size:        635    3757
 
AhdI GACnn_n'nnGTC

Cuts at:      0     162    2864    4356    4392 
   Size:        162    2702    1492      36

  Fragments arranged by size: 

                2702    1492     162      36
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3724    4392 
   Size:       3724     668
 
ApaI G_GGCC'C

Cuts at:      0    2015    4392 
   Size:       2015    2377
 
ApoI r'AATT_y

Cuts at:      0     577    1024    1309    1742    2389    4392 
   Size:        577     447     285     433     647    2003

  Fragments arranged by size: 

                2003     647     577     447     433     285
 
AvaI C'yCGr_G

Cuts at:      0    2519    4392 
   Size:       2519    1873
 
BanI G'GyrC_C

Cuts at:      0    4291    4392 
   Size:       4291     101
 
BanII G_rGCy'C

Cuts at:      0     835    1727    2015    3551    3599    3923    4392 
   Size:        835     892     288    1536      48     324     469

  Fragments arranged by size: 

                1536     892     835     469     324     288      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      20     419    2309    2765    2888    2918    4010    4097 
   Size:         20     399    1890     456     123      30    1092      87

Cuts at:   4097    4392 
   Size:        295

  Fragments arranged by size: 

                1890    1092     456     399     295     123      87      30
                  20
 
BbvCI CC'TCA_GC

Cuts at:      0     329     928    3404    4392 
   Size:        329     599    2476     988

  Fragments arranged by size: 

                2476     988     599     329
 
BclI T'GATC_A

Cuts at:      0    4217    4392 
   Size:       4217     175
 
BfrBI ATG'CAT

Cuts at:      0     736    1047    4392 
   Size:        736     311    3345

  Fragments arranged by size: 

                3345     736     311
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1186    4392 
   Size:       1186    3206
 
BlpI GC'TnA_GC

Cuts at:      0    1971    3426    3812    3854    3875    4392 
   Size:       1971    1455     386      42      21     517

  Fragments arranged by size: 

                1971    1455     517     386      42      21
 
Bme1580I G_kGCm'C

Cuts at:      0    2015    4392 
   Size:       2015    2377
 
BmgBI CAC'GTC

Cuts at:      0      33    4392 
   Size:         33    4359
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     126    2009    4392 
   Size:        126    1883    2383

  Fragments arranged by size: 

                2383    1883     126
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1078    2545    2631    4392 
   Size:       1078    1467      86    1761

  Fragments arranged by size: 

                1761    1467    1078      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252    2108    2375    3122    3406    4392 
   Size:        252    1856     267     747     284     986

  Fragments arranged by size: 

                1856     986     747     284     267     252
 
Bpu10I CC'TnA_GC

Cuts at:      0     329     919     928    3404    4254    4392 
   Size:        329     590       9    2476     850     138

  Fragments arranged by size: 

                2476     850     590     329     138       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2801    4392 
   Size:       2801    1591
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     149    1156    2464    2836    2851    4392 
   Size:        149    1007    1308     372      15    1541

  Fragments arranged by size: 

                1541    1308    1007     372     149      15
 
BsaAI yAC'GTr

Cuts at:      0    3528    4392 
   Size:       3528     864
 
BsaHI Gr'CG_yC

Cuts at:      0    1650    4328    4392 
   Size:       1650    2678      64

  Fragments arranged by size: 

                2678    1650      64
 
BsaWI w'CCGG_w

Cuts at:      0     122     635    4392 
   Size:        122     513    3757

  Fragments arranged by size: 

                3757     513     122
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     250     280    3246    3276    3550    3580    4392 
   Size:        250      30    2966      30     274      30     812

  Fragments arranged by size: 

                2966     812     274     250      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2824    2854    2869    4392 
   Size:       2824      30      15    1523

  Fragments arranged by size: 

                2824    1523      30      15
 
BseYI C'CCAG_C

Cuts at:      0    1456    1461    2401    3289    3507    4392 
   Size:       1456       5     940     888     218     885

  Fragments arranged by size: 

                1456     940     888     885     218       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421    1341    3500    4013    4392 
   Size:        421     920    2159     513     379

  Fragments arranged by size: 

                2159     920     513     421     379
 
BsiEI CG_ry'CG

Cuts at:      0     636    4392 
   Size:        636    3756
 
BsiHKAI G_wGCw'C

Cuts at:      0    1037    1370    1727    2588    4255    4392 
   Size:       1037     333     357     861    1667     137

  Fragments arranged by size: 

                1667    1037     861     357     333     137
 
BsmI GAATG_Cn'

Cuts at:      0     738    3631    4159    4183    4392 
   Size:        738    2893     528      24     209

  Fragments arranged by size: 

                2893     738     528     209      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     835    1037    1370    1727    2015    2588    3551    3599 
   Size:        835     202     333     357     288     573     963      48

Cuts at:   3599    3923    4255    4392 
   Size:        324     332     137

  Fragments arranged by size: 

                 963     835     573     357     333     332     324     288
                 202     137      48
 
BspHI T'CATG_A

Cuts at:      0    2598    4392 
   Size:       2598    1794
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3985    4392 
   Size:       3985     407
 
BsrDI GCAATG_nn'

Cuts at:      0     980    2472    4392 
   Size:        980    1492    1920

  Fragments arranged by size: 

                1920    1492     980
 
BsrFI r'CCGG_y

Cuts at:      0     527     635    4392 
   Size:        527     108    3757

  Fragments arranged by size: 

                3757     527     108
 
BssSI C'ACGA_G

Cuts at:      0     152    2432    4392 
   Size:        152    2280    1960

  Fragments arranged by size: 

                2280    1960     152
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3023    3517    3724    4392 
   Size:       3023     494     207     668

  Fragments arranged by size: 

                3023     668     494     207
 
BstEII G'GTnAC_C

Cuts at:      0     106    4392 
   Size:        106    4286
 
BstYI r'GATC_y

Cuts at:      0     204     454    2362    2979    4392 
   Size:        204     250    1908     617    1413

  Fragments arranged by size: 

                1908    1413     617     250     204
 
BtgI C'CryG_G

Cuts at:      0     199     558    2862    3250    4392 
   Size:        199     359    2304     388    1142

  Fragments arranged by size: 

                2304    1142     388     359     199
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3618    3653    4392 
   Size:       3618      35     739

  Fragments arranged by size: 

                3618     739      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     564    1095    4392 
   Size:        564     531    3297

  Fragments arranged by size: 

                3297     564     531
 
EaeI y'GGCC_r

Cuts at:      0     420    1695    3296    4392 
   Size:        420    1275    1601    1096

  Fragments arranged by size: 

                1601    1275    1096     420
 
EarI CTCTTCn'nnn_

Cuts at:      0      60     609    1819    3455    4019    4040    4348    4392 
   Size:         60     549    1210    1636     564      21     308      44

  Fragments arranged by size: 

                1636    1210     564     549     308      60      44      21
            
 
EcoICRI GAG'CTC

Cuts at:      0    1725    4392 
   Size:       1725    2667
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     431     527    1270    2108    2123    2375    2718 
   Size:        252     179      96     743     838      15     252     343

Cuts at:   2718    3122    3406    3914    4043    4064    4392 
   Size:        404     284     508     129      21     328

  Fragments arranged by size: 

                 838     743     508     404     343     328     284     252
                 252     179     129      96      21      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     328    2273    4392 
   Size:        328    1945    2119

  Fragments arranged by size: 

                2119    1945     328
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2011    3563    4392 
   Size:       2011    1552     829

  Fragments arranged by size: 

                2011    1552     829
 
EcoRI G'AATT_C

Cuts at:      0    2389    4392 
   Size:       2389    2003
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     888    1356    1779    4392 
   Size:        888     468     423    2613

  Fragments arranged by size: 

                2613     888     468     423
 
HaeII r_GCGC'y

Cuts at:      0     807    4392 
   Size:        807    3585
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     250     271     282     286     303     318 
   Size:        148      32      70      21      11       4      17      15

Cuts at:    318     409     441     792     824     828     860    1927    1959 
   Size:         91      32     351      32       4      32    1067      32

Cuts at:   1959    2850    2882    3547    3579    4342    4374    4392 
   Size:        891      32     665      32     763      32      18

  Fragments arranged by size: 

                1067     891     763     665     351     148      91      70
                  32      32      32      32      32      32      32      32
                  21      18      17      15      11       4       4
 
HincII GTy'rAC

Cuts at:      0    3033    3504    4392 
   Size:       3033     471     888

  Fragments arranged by size: 

                3033     888     471
 
HindIII A'AGCT_T

Cuts at:      0     234    4392 
   Size:        234    4158
 
MfeI C'AATT_G

Cuts at:      0     959    2876    4392 
   Size:        959    1917    1516

  Fragments arranged by size: 

                1917    1516     959
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1107    4392 
   Size:       1107    3285
 
MscI TGG'CCA

Cuts at:      0     422    4392 
   Size:        422    3970
 
MslI CAynn'nnrTG

Cuts at:      0     907     984    1418    3542    4392 
   Size:        907      77     434    2124     850

  Fragments arranged by size: 

                2124     907     850     434      77
 
MspA1I CmG'CkG

Cuts at:      0     161     201     717    3111    3724    4392 
   Size:        161      40     516    2394     613     668

  Fragments arranged by size: 

                2394     668     613     516     161      40
 
NcoI C'CATG_G

Cuts at:      0    2862    4392 
   Size:       2862    1530
 
NdeI CA'TA_TG

Cuts at:      0     981    1947    3356    4392 
   Size:        981     966    1409    1036

  Fragments arranged by size: 

                1409    1036     981     966
 
NsiI A_TGCA'T

Cuts at:      0     738    1049    4392 
   Size:        738     311    3343

  Fragments arranged by size: 

                3343     738     311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315    1095    3130    4392 
   Size:        315     780    2035    1262

  Fragments arranged by size: 

                2035    1262     780     315
 
PfoI T'CCnGG_A

Cuts at:      0     356    2352    4392 
   Size:        356    1996    2040

  Fragments arranged by size: 

                2040    1996     356
 
PpuMI rG'GwC_Cy

Cuts at:      0    3563    4392 
   Size:       3563     829
 
PspOMI G'GGCC_C

Cuts at:      0    2011    4392 
   Size:       2011    2381
 
PspXI vC'TCGA_Gb

Cuts at:      0    2519    4392 
   Size:       2519    1873
 
PstI C_TGCA'G

Cuts at:      0    3270    3729    3918    4392 
   Size:       3270     459     189     474

  Fragments arranged by size: 

                3270     474     459     189
 
PvuII CAG'CTG

Cuts at:      0    3111    3724    4392 
   Size:       3111     613     668

  Fragments arranged by size: 

                3111     668     613
 
SacI G_AGCT'C

Cuts at:      0    1727    4392 
   Size:       1727    2665
 
SacII CC_GC'GG

Cuts at:      0     202    4392 
   Size:        202    4190
 
SapI GCTCTTCn'nnn_

Cuts at:      0     609    4019    4040    4348    4392 
   Size:        609    3410      21     308      44

  Fragments arranged by size: 

                3410     609     308      44      21
 
ScaI AGT'ACT

Cuts at:      0    4315    4392 
   Size:       4315      77
 
SfcI C'TryA_G

Cuts at:      0     375    3266    3725    3914    4392 
   Size:        375    2891     459     189     478

  Fragments arranged by size: 

                2891     478     459     375     189
 
SgrAI Cr'CCGG_yG

Cuts at:      0     527    4392 
   Size:        527    3865
 
SmlI C'TyrA_G

Cuts at:      0    2519    2816    4392 
   Size:       2519     297    1576

  Fragments arranged by size: 

                2519    1576     297
 
StyI C'CwwG_G

Cuts at:      0    1184    2862    4392 
   Size:       1184    1678    1530

  Fragments arranged by size: 

                1678    1530    1184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3082    3472    4392 
   Size:       3082     390     920

  Fragments arranged by size: 

                3082     920     390
 
TatI w'GTAC_w

Cuts at:      0    3044    4313    4392 
   Size:       3044    1269      79

  Fragments arranged by size: 

                3044    1269      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     632     664    3055    3087    3246    3278    4392 
   Size:        632      32    2391      32     159      32    1114

  Fragments arranged by size: 

                2391    1114     632     159      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2308    2887    4392 
   Size:       2308     579    1505

  Fragments arranged by size: 

                2308    1505     579
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     100     158    4392 
   Size:        100      58    4234

  Fragments arranged by size: 

                4234     100      58
 
XhoI C'TCGA_G

Cuts at:      0    2519    4392 
   Size:       2519    1873
 
XmnI GAAnn'nnTTC

Cuts at:      0    4315    4392 
   Size:       4315      77
 
ZraI GAC'GTC

Cuts at:      0    4329    4392 
   Size:       4329      63
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI     AgeI     AhdI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstYI     BtgI    CspCI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NsiI    PflMI     PfoI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI    SacII     SapI     ScaI     SfcI
    SgrAI     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII   AflIII     AleI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BglII     BmtI    BsaBI    BsiWI    BsmBI    BspEI
    BsrBI    BsrGI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DraI     DrdI     EagI     EciI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MluI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI
     XmaI
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