Restriction map for pF1KSDA0278
 (Circular) (Six-base) MAPSORT of: pF1KSDA0278.seq  Check: 140  from: 1  to: 4325



pF1KSDA0278  4325 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    177     278     282     804     177 
   Size:        101       4     522    3698

  Fragments arranged by size: 

                3698     522     101       4
 
AccI GT'mk_AC

Cuts at:   1363    1363 
   Size:       4325
 
Acc65I G'GTAC_C

Cuts at:   1341    1341 
   Size:       4325
 
AclI AA'CG_TT

Cuts at:   4165    4165 
   Size:       4325
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    328    2150    2582    3329    3597     328 
   Size:       1822     432     747     268    1056

  Fragments arranged by size: 

                1822    1056     747     432     268
 
AfeI AGC'GCT

Cuts at:    382     445     499     382 
   Size:         63      54    4208

  Fragments arranged by size: 

                4208      63      54
 
AflIII A'CryG_T

Cuts at:    467    1677    2781    3758     467 
   Size:       1210    1104     977    1034

  Fragments arranged by size: 

                1210    1104    1034     977
 
AgeI A'CCGG_T

Cuts at:    320    2713     320 
   Size:       2393    1932
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1204    1204 
   Size:       4325
 
AlwNI CAG_nnn'CTG

Cuts at:    664    1029    1630    3197     664 
   Size:        365     601    1567    1792

  Fragments arranged by size: 

                1792    1567     601     365
 
ApaLI G'TGCA_C

Cuts at:   3095    3095 
   Size:       4325
 
ApoI r'AATT_y

Cuts at:   1329    3678    3934    1329 
   Size:       2349     256    1720

  Fragments arranged by size: 

                2349    1720     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4325
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4325
 
AvaI C'yCGr_G

Cuts at:    169     589     676     776     965    1309    1345     169 
   Size:        420      87     100     189     344      36    3149

  Fragments arranged by size: 

                3149     420     344     189     100      87      36
 
BamHI G'GATC_C

Cuts at:   1350    1350 
   Size:       4325
 
BanI G'GyrC_C

Cuts at:    266    1105    1341    1755    2003    2038     266 
   Size:        839     236     414     248      35    2553

  Fragments arranged by size: 

                2553     839     414     248     236      35
 
BanII G_rGCy'C

Cuts at:    158     689     904    1339    2369     158 
   Size:        531     215     435    1030    2114

  Fragments arranged by size: 

                2114    1030     531     435     215
 
BbeI G_GCGC'C

Cuts at:   1759    2007    1759 
   Size:        248    4077
 
BciVI GTATCCnnnnn_n'

Cuts at:   2223    2984    4325    2223 
   Size:        761    1341    2223

  Fragments arranged by size: 

                2223    1341     761
 
BclI T'GATC_A

Cuts at:   1845    1845 
   Size:       4325
 
BglI GCCn_nnn'nGGC

Cuts at:   1219    1219 
   Size:       4325
 
BglII A'GATC_T

Cuts at:    761    1840    3877     761 
   Size:       1079    2037    1209

  Fragments arranged by size: 

                2037    1209    1079
 
BlpI GC'TnA_GC

Cuts at:   1437    1437 
   Size:       4325
 
Bme1580I G_kGCm'C

Cuts at:    365     958    1108    1950    2043    3099     365 
   Size:        593     150     842      93    1056    1591

  Fragments arranged by size: 

                1591    1056     842     593     150      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    924    1537    1618    1953    4097     924 
   Size:        613      81     335    2144    1152

  Fragments arranged by size: 

                2144    1152     613     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2692    2692 
   Size:       4325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    262     358     460     514     535    1180    1222     262 
   Size:         96     102      54      21     645      42    3365

  Fragments arranged by size: 

                3365     645     102      96      54      42      21
 
Bpu10I CC'TnA_GC

Cuts at:    787     787 
   Size:       4325
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1108    1501    2380    2872    3170    1108 
   Size:        393     879     492     298    2263

  Fragments arranged by size: 

                2263     879     492     393     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    418    1249    4029     418 
   Size:        831    2780     714

  Fragments arranged by size: 

                2780     831     714
 
BsaAI yAC'GTr

Cuts at:    249     360     468    1065    1098    1317    1678     249 
   Size:        111     108     597      33     219     361    2896

  Fragments arranged by size: 

                2896     597     361     219     111     108      33
 
BsaBI GATnn'nnATC

Cuts at:      6    1839    3876       6 
   Size:       1833    2037     455

  Fragments arranged by size: 

                2037    1833     455
 
BsaHI Gr'CG_yC

Cuts at:   1756    2004    2706    4202    1756 
   Size:        248     702    1496    1879

  Fragments arranged by size: 

                1879    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    140     320    1298    1514    2035    2713    2987    3134    3545 
   Size:        180     978     216     521     678     274     147     411

Cuts at:   3545     140 
   Size:        920

  Fragments arranged by size: 

                 978     920     678     521     411     274     216     180
                 147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    814     844    1093    1096     814 
   Size:         30     249       3    4043

  Fragments arranged by size: 

                4043     249      30       3
 
BseYI C'CCAG_C

Cuts at:    446     500     657     698     739    1304    1750    2508    3085 
   Size:         54     157      41      41     565     446     758     577

Cuts at:   3085    3581     446 
   Size:        496    1190

  Fragments arranged by size: 

                1190     758     577     565     496     446     157      54
                  41      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    301    1210     301 
   Size:        909    3416
 
BsiEI CG_ry'CG

Cuts at:    104     325     329     510    1214    1223    1867    1913    3121 
   Size:        221       4     181     704       9     644      46    1208

Cuts at:   3121     104 
   Size:       1308

  Fragments arranged by size: 

                1308    1208     704     644     221     181      46       9
                   4
 
BsiHKAI G_wGCw'C

Cuts at:    248    1339    2117    2307    3099     248 
   Size:       1091     778     190     792    1474

  Fragments arranged by size: 

                1474    1091     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    158     248     365     689     904     958    1108    1339    1950 
   Size:         90     117     324     215      54     150     231     611

Cuts at:   1950    2043    2117    2307    2369    3099     158 
   Size:         93      74     190      62     730    1384

  Fragments arranged by size: 

                1384     730     611     324     231     215     190     150
                 117      93      90      74      62      54
 
BspEI T'CCGG_A

Cuts at:   3545    3545 
   Size:       4325
 
BspHI T'CATG_A

Cuts at:   3501    3501 
   Size:       4325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    177     278     282     804    1001    1375    1589    1891    2272 
   Size:        101       4     522     197     374     214     302     381

Cuts at:   2272     177 
   Size:       2230

  Fragments arranged by size: 

                2230     522     381     374     302     214     197     101
                   4
 
BsrBI CCG'CTC

Cuts at:     50    2617    2671    4155    4320      50 
   Size:       2567      54    1484     165      55

  Fragments arranged by size: 

                2567    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2237    2237 
   Size:       4325
 
BsrFI r'CCGG_y

Cuts at:    263     320     870    1218    1222    2323    2504    2713     263 
   Size:         57     550     348       4    1101     181     209    1875

  Fragments arranged by size: 

                1875    1101     550     348     209     181      57       4
            
 
BssSI C'ACGA_G

Cuts at:    366     604    2596    2954     366 
   Size:        238    1992     358    1737

  Fragments arranged by size: 

                1992    1737     358     238
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1702    1702 
   Size:       4325
 
BstBI TT'CG_AA

Cuts at:    110    2686     110 
   Size:       2576    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    132     294    1158     132 
   Size:        162     864    3299

  Fragments arranged by size: 

                3299     864     162
 
BstYI r'GATC_y

Cuts at:      1     761     848    1350    1817    1840    2175    2421    3433 
   Size:        760      87     502     467      23     335     246    1012

Cuts at:   3433    3519    3531    3877       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     760     502     467     449     346     335     246
                  87      86      23      12
 
BtgI C'CryG_G

Cuts at:    125     175     521     572     712    1286     125 
   Size:         50     346      51     140     574    3164

  Fragments arranged by size: 

                3164     574     346     140      51      50
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    414    2275    2456    2619     414 
   Size:       1861     181     163    2120

  Fragments arranged by size: 

                2120    1861     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1568    1568 
   Size:       4325
 
DraI TTT'AAA

Cuts at:   1324    1324 
   Size:       4325
 
DrdI GACnn_nn'nnGTC

Cuts at:    628    2031    2889     628 
   Size:       1403     858    2064

  Fragments arranged by size: 

                2064    1403     858
 
EaeI y'GGCC_r

Cuts at:    191     275     872    1211    1220    1289    1910    2084    2475 
   Size:         84     597     339       9      69     621     174     391

Cuts at:   2475    2502    3847     191 
   Size:         27    1345     669

  Fragments arranged by size: 

                1345     669     621     597     391     339     174      84
                  69      27       9
 
EagI C'GGCC_G

Cuts at:   1211    1220    1910    1211 
   Size:          9     690    3626

  Fragments arranged by size: 

                3626     690       9
 
EarI CTCTTCn'nnn_

Cuts at:   2348    2558    2348 
   Size:        210    4115
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    270    1267    2533    2843    2989    4135     270 
   Size:        997    1266     310     146    1146     460

  Fragments arranged by size: 

                1266    1146     997     460     310     146
 
EcoICRI GAG'CTC

Cuts at:   1337    1337 
   Size:       4325
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    262     328     358     460     514     535    1180    1222    2150 
   Size:         66      30     102      54      21     645      42     928

Cuts at:   2150    2582    3329    3597     262 
   Size:        432     747     268     990

  Fragments arranged by size: 

                 990     928     747     645     432     268     102      66
                  54      42      30      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1204    1204 
   Size:       4325
 
EcoO109I rG'GnC_Cy

Cuts at:    586     717     771    1044    1464     586 
   Size:        131      54     273     420    3447

  Fragments arranged by size: 

                3447     420     273     131      54
 
EcoRI G'AATT_C

Cuts at:   1329    1329 
   Size:       4325
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2344    2344 
   Size:       4325
 
FseI GG_CCGG'CC

Cuts at:    874    1226     874 
   Size:        352    3973
 
FspI TGC'GCA

Cuts at:   2106    2106 
   Size:       4325
 
HaeII r_GCGC'y

Cuts at:    384     447     501    1759    2007    3029    4008     384 
   Size:         63      54    1258     248    1022     979     701

  Fragments arranged by size: 

                1258    1022     979     701     248      63      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    840     872     959     991    2167    2197    2199    2229     840 
   Size:         32      87      32    1176      30       2      30    2936

  Fragments arranged by size: 

                2936    1176      87      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1364    1364 
   Size:       4325
 
KasI G'GCGC_C

Cuts at:   1755    2003    1755 
   Size:        248    4077
 
KpnI G_GTAC'C

Cuts at:   1345    1345 
   Size:       4325
 
MluI A'CGCG_T

Cuts at:   3758    3758 
   Size:       4325
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2996    3180    2996 
   Size:        184    4141
 
MscI TGG'CCA

Cuts at:    193    1291    2086     193 
   Size:       1098     795    2432

  Fragments arranged by size: 

                2432    1098     795
 
MslI CAynn'nnrTG

Cuts at:   1186    2314    2441    1186 
   Size:       1128     127    3070

  Fragments arranged by size: 

                3070    1128     127
 
MspA1I CmG'CkG

Cuts at:    149     177     416     664     981    1017    1436    1750    2110 
   Size:         28     239     248     317      36     419     314     360

Cuts at:   2110    3123    3368     149 
   Size:       1013     245    1106

  Fragments arranged by size: 

                1106    1013     419     360     317     314     248     245
                 239      36      28
 
NaeI GCC'GGC

Cuts at:    872    1220    1224    2506     872 
   Size:        348       4    1282    2691

  Fragments arranged by size: 

                2691    1282     348       4
 
NarI GG'CG_CC

Cuts at:   1756    2004    1756 
   Size:        248    4077
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4325
 
NgoMIV G'CCGG_C

Cuts at:    870    1218    1222    2504     870 
   Size:        348       4    1282    2691

  Fragments arranged by size: 

                2691    1282     348       4
 
NspI r_CATG'y

Cuts at:   1378    1878    2785    1378 
   Size:        500     907    2918

  Fragments arranged by size: 

                2918     907     500
 
PciI A'CATG_T

Cuts at:   2781    2781 
   Size:       4325
 
PflMI CCAn_nnn'nTGG

Cuts at:    664     881    1098     664 
   Size:        217     217    3891

  Fragments arranged by size: 

                3891     217     217
 
PmeI GTTT'AAAC

Cuts at:   1324    1324 
   Size:       4325
 
PmlI CAC'GTG

Cuts at:    249     468     249 
   Size:        219    4106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3464    3496    3464 
   Size:         32    4293
 
PpuMI rG'GwC_Cy

Cuts at:    586     717     771    1044     586 
   Size:        131      54     273    3867

  Fragments arranged by size: 

                3867     273     131      54
 
PstI C_TGCA'G

Cuts at:    215     944    1115    1372    2057     215 
   Size:        729     171     257     685    2483

  Fragments arranged by size: 

                2483     729     685     257     171
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4325
 
PvuII CAG'CTG

Cuts at:   1750    2110    1750 
   Size:        360    3965
 
RsrII CG'GwC_CG

Cuts at:   2520    2520 
   Size:       4325
 
SacI G_AGCT'C

Cuts at:   1339    1339 
   Size:       4325
 
SacII CC_GC'GG

Cuts at:    178     178 
   Size:       4325
 
SalI G'TCGA_C

Cuts at:   1362    1362 
   Size:       4325
 
SanDI GG'GwC_CC

Cuts at:    586     717     586 
   Size:        131    4194
 
SapI GCTCTTCn'nnn_

Cuts at:   2348    2558    2348 
   Size:        210    4115
 
SbfI CC_TGCA'GG

Cuts at:   1372    1372 
   Size:       4325
 
ScaI AGT'ACT

Cuts at:    805     835    3842     805 
   Size:         30    3007    1288

  Fragments arranged by size: 

                3007    1288      30
 
SfcI C'TryA_G

Cuts at:     33     211     940    1111    1368    2053    3046    3237      33 
   Size:        178     729     171     257     685     993     191    1121

  Fragments arranged by size: 

                1121     993     729     685     257     191     178     171
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1219    1219 
   Size:       4325
 
SfoI GGC'GCC

Cuts at:   1757    2005    1757 
   Size:        248    4077
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4325
 
SmaI CCC'GGG

Cuts at:    171     678    1347     171 
   Size:        507     669    3149

  Fragments arranged by size: 

                3149     669     507
 
SmlI C'TyrA_G

Cuts at:    776    1123    1480    2395    2887    3149     776 
   Size:        347     357     915     492     262    1952

  Fragments arranged by size: 

                1952     915     492     357     347     262
 
SnaBI TAC'GTA

Cuts at:   1317    1317 
   Size:       4325
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4325
 
SphI G_CATG'C

Cuts at:   1378    1878    1378 
   Size:        500    3825
 
SrfI GCCC'GGGC

Cuts at:    678     678 
   Size:       4325
 
StuI AGG'CCT

Cuts at:    403     403 
   Size:       4325
 
StyI C'CwwG_G

Cuts at:    125    1459    1813     125 
   Size:       1334     354    2637

  Fragments arranged by size: 

                2637    1334     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    315     906    1908    2041    2500    2709     315 
   Size:        591    1002     133     459     209    1931

  Fragments arranged by size: 

                1931    1002     591     459     209     133
 
TatI w'GTAC_w

Cuts at:    803     833     944    3840     803 
   Size:         30     111    2896    1288

  Fragments arranged by size: 

                2896    1288     111      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    553     585    3842    3874     553 
   Size:         32    3257      32    1004

  Fragments arranged by size: 

                3257    1004      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2122    2122 
   Size:       4325
 
XbaI T'CTAG_A

Cuts at:   1356    1356 
   Size:       4325
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    435     747    1820     435 
   Size:        312    1073    2940

  Fragments arranged by size: 

                2940    1073     312
 
XhoI C'TCGA_G

Cuts at:    776     776 
   Size:       4325
 
XmaI C'CCGG_G

Cuts at:    169     676    1345     169 
   Size:        507     669    3149

  Fragments arranged by size: 

                3149     669     507
 
XmnI GAAnn'nnTTC

Cuts at:    934     934 
   Size:       4325
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AloI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsaXI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoRV    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SexAI    SgrAI     SspI
     SwaI     ZraI
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