Restriction map for pF1KSDA0253
 (Linear) (Six-base) MAPSORT of: KIAA0253.seq  Check: 3718  from: 1  to: 2127



KIAA0253.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     694    2127 
   Size:        694    1433
 
AccI GT'mk_AC

Cuts at:      0    1031    2127 
   Size:       1031    1096
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     702     947    1439    1503    2127 
   Size:        702     245     492      64     624

  Fragments arranged by size: 

                 702     624     492     245      64
 
AflII C'TTAA_G

Cuts at:      0    1107    2127 
   Size:       1107    1020
 
AhdI GACnn_n'nnGTC

Cuts at:      0     750    1626    2127 
   Size:        750     876     501

  Fragments arranged by size: 

                 876     750     501
 
AleI CACnn'nnGTG

Cuts at:      0    1334    2127 
   Size:       1334     793
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     105    2127 
   Size:        105    2022
 
AlwNI CAG_nnn'CTG

Cuts at:      0     883    1144    1447    2116    2127 
   Size:        883     261     303     669      11

  Fragments arranged by size: 

                 883     669     303     261      11
 
ApaI G_GGCC'C

Cuts at:      0    1822    2127 
   Size:       1822     305
 
ApoI r'AATT_y

Cuts at:      0     492    1716    2127 
   Size:        492    1224     411

  Fragments arranged by size: 

                1224     492     411
 
AseI AT'TA_AT

Cuts at:      0    1388    2127 
   Size:       1388     739
 
AvaI C'yCGr_G

Cuts at:      0      33    1020    1745    2102    2127 
   Size:         33     987     725     357      25

  Fragments arranged by size: 

                 987     725     357      33      25
 
BamHI G'GATC_C

Cuts at:      0    1761    2127 
   Size:       1761     366
 
BanI G'GyrC_C

Cuts at:      0     942    1336    2127 
   Size:        942     394     791

  Fragments arranged by size: 

                 942     791     394
 
BanII G_rGCy'C

Cuts at:      0    1299    1417    1822    1924    2127 
   Size:       1299     118     405     102     203

  Fragments arranged by size: 

                1299     405     203     118     102
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     182     527     975    2127 
   Size:        182     345     448    1152

  Fragments arranged by size: 

                1152     448     345     182
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1736    1770    2127 
   Size:       1736      34     357

  Fragments arranged by size: 

                1736     357      34
 
BclI T'GATC_A

Cuts at:      0    2009    2127 
   Size:       2009     118
 
BfrBI ATG'CAT

Cuts at:      0     663    2127 
   Size:        663    1464
 
BglI GCCn_nnn'nGGC

Cuts at:      0     880    2127 
   Size:        880    1247
 
BlpI GC'TnA_GC

Cuts at:      0     615    1411    2127 
   Size:        615     796     716

  Fragments arranged by size: 

                 796     716     615
 
Bme1580I G_kGCm'C

Cuts at:      0     421    1822    1974    2127 
   Size:        421    1401     152     153

  Fragments arranged by size: 

                1401     421     153     152
 
BmgBI CAC'GTC

Cuts at:      0    1486    2127 
   Size:       1486     641
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     377     806    2127 
   Size:        377     429    1321

  Fragments arranged by size: 

                1321     429     377
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1656    2127 
   Size:        313    1343     471

  Fragments arranged by size: 

                1343     471     313
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2023    2127 
   Size:       2023     104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52    2127 
   Size:         52    2075
 
BsaAI yAC'GTr

Cuts at:      0     222    2127 
   Size:        222    1905
 
BsaBI GATnn'nnATC

Cuts at:      0     121    2127 
   Size:        121    2006
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250    2127 
   Size:        250    1877
 
BseYI C'CCAG_C

Cuts at:      0     498     911    1092    1256    1479    2127 
   Size:        498     413     181     164     223     648

  Fragments arranged by size: 

                 648     498     413     223     181     164
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111    1081    1856    2127 
   Size:        111     970     775     271

  Fragments arranged by size: 

                 970     775     271     111
 
BsiHKAI G_wGCw'C

Cuts at:      0    1299    1491    1924    2127 
   Size:       1299     192     433     203

  Fragments arranged by size: 

                1299     433     203     192
 
BsmI GAATG_Cn'

Cuts at:      0    1827    2127 
   Size:       1827     300
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1831    2127 
   Size:       1831     296
 
Bsp1286I G_dGCh'C

Cuts at:      0     421    1299    1417    1491    1822    1924    1974    2127 
   Size:        421     878     118      74     331     102      50     153

  Fragments arranged by size: 

                 878     421     331     153     118     102      74      50
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     694    2127 
   Size:         73     621    1433

  Fragments arranged by size: 

                1433     621      73
 
BsrDI GCAATG_nn'

Cuts at:      0     510     975    2093    2127 
   Size:        510     465    1118      34

  Fragments arranged by size: 

                1118     510     465      34
 
BsrGI T'GTAC_A

Cuts at:      0     411    1698    2127 
   Size:        411    1287     429

  Fragments arranged by size: 

                1287     429     411
 
BstEII G'GTnAC_C

Cuts at:      0    1551    2127 
   Size:       1551     576
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    1458    1479    2127 
   Size:        843     615      21     648

  Fragments arranged by size: 

                 843     648     615      21
 
BstYI r'GATC_y

Cuts at:      0    1140    1185    1761    1791    2127 
   Size:       1140      45     576      30     336

  Fragments arranged by size: 

                1140     576     336      45      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     393    1237    1612    1624    2127 
   Size:        393     844     375      12     503

  Fragments arranged by size: 

                 844     503     393     375      12
 
BtgI C'CryG_G

Cuts at:      0    1472    2127 
   Size:       1472     655
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1647    1971    2127 
   Size:       1647     324     156

  Fragments arranged by size: 

                1647     324     156
 
BtsI GCAGTG_nn'

Cuts at:      0     371     471     586     677     899    1373    1445    1759 
   Size:        371     100     115      91     222     474      72     314

Cuts at:   1759    2127 
   Size:        368

  Fragments arranged by size: 

                 474     371     368     314     222     115     100      91
                  72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1677    1712    2127 
   Size:       1677      35     415

  Fragments arranged by size: 

                1677     415      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     637    2127 
   Size:        637    1490
 
EaeI y'GGCC_r

Cuts at:      0    1193    1469    2127 
   Size:       1193     276     658

  Fragments arranged by size: 

                1193     658     276
 
EarI CTCTTCn'nnn_

Cuts at:      0    1201    1415    1655    2127 
   Size:       1201     214     240     472

  Fragments arranged by size: 

                1201     472     240     214
 
EcoICRI GAG'CTC

Cuts at:      0    1297    1922    2127 
   Size:       1297     625     205

  Fragments arranged by size: 

                1297     625     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     702     947    1439    1503    1656    2127 
   Size:        313     389     245     492      64     153     471

  Fragments arranged by size: 

                 492     471     389     313     245     153      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     392    1615    2127 
   Size:        392    1223     512

  Fragments arranged by size: 

                1223     512     392
 
EcoO109I rG'GnC_Cy

Cuts at:      0     240    1425    1455    1620    1628    1818    1819    1886 
   Size:        240    1185      30     165       8     190       1      67

Cuts at:   1886    2127 
   Size:        241

  Fragments arranged by size: 

                1185     241     240     190     165      67      30       8
                   1
 
EcoRI G'AATT_C

Cuts at:      0     492    2127 
   Size:        492    1635
 
EcoRV GAT'ATC

Cuts at:      0    1965    2127 
   Size:       1965     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     736     768    1233    1265    1612    1644    2127 
   Size:        736      32     465      32     347      32     483

  Fragments arranged by size: 

                 736     483     465     347      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1077    1737    2127 
   Size:       1077     660     390

  Fragments arranged by size: 

                1077     660     390
 
HindIII A'AGCT_T

Cuts at:      0     929    2127 
   Size:        929    1198
 
MscI TGG'CCA

Cuts at:      0    1195    1471    2127 
   Size:       1195     276     656

  Fragments arranged by size: 

                1195     656     276
 
MslI CAynn'nnrTG

Cuts at:      0     662     688    1334    1390    1832    2127 
   Size:        662      26     646      56     442     295

  Fragments arranged by size: 

                 662     646     442     295      56      26
 
MspA1I CmG'CkG

Cuts at:      0     915    1955    2127 
   Size:        915    1040     172

  Fragments arranged by size: 

                1040     915     172
 
NsiI A_TGCA'T

Cuts at:      0     665    2127 
   Size:        665    1462
 
NspI r_CATG'y

Cuts at:      0     663     667     693     777    2127 
   Size:        663       4      26      84    1350

  Fragments arranged by size: 

                1350     663      84      26       4
 
PasI CC'CwG_GG

Cuts at:      0     877    2127 
   Size:        877    1250
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     427    2127 
   Size:        427    1700
 
PpuMI rG'GwC_Cy

Cuts at:      0     240    1425    1620    1628    2127 
   Size:        240    1185     195       8     499

  Fragments arranged by size: 

                1185     499     240     195       8
 
PspOMI G'GGCC_C

Cuts at:      0    1818    2127 
   Size:       1818     309
 
PspXI vC'TCGA_Gb

Cuts at:      0    1020    2127 
   Size:       1020    1107
 
PstI C_TGCA'G

Cuts at:      0     478    1283    2127 
   Size:        478     805     844

  Fragments arranged by size: 

                 844     805     478
 
PvuII CAG'CTG

Cuts at:      0     915    2127 
   Size:        915    1212
 
RsrII CG'GwC_CG

Cuts at:      0    1842    2127 
   Size:       1842     285
 
SacI G_AGCT'C

Cuts at:      0    1299    1924    2127 
   Size:       1299     625     203

  Fragments arranged by size: 

                1299     625     203
 
ScaI AGT'ACT

Cuts at:      0    1804    2127 
   Size:       1804     323
 
SexAI A'CCwGG_T

Cuts at:      0    1175    2127 
   Size:       1175     952
 
SfcI C'TryA_G

Cuts at:      0     244     474    1279    2127 
   Size:        244     230     805     848

  Fragments arranged by size: 

                 848     805     244     230
 
SmaI CCC'GGG

Cuts at:      0      35    2104    2127 
   Size:         35    2069      23

  Fragments arranged by size: 

                2069      35      23
 
SmlI C'TyrA_G

Cuts at:      0    1020    1107    2002    2127 
   Size:       1020      87     895     125

  Fragments arranged by size: 

                1020     895     125      87
 
SphI G_CATG'C

Cuts at:      0     667     693     777    2127 
   Size:        667      26      84    1350

  Fragments arranged by size: 

                1350     667      84      26
 
SspI AAT'ATT

Cuts at:      0    1354    2127 
   Size:       1354     773
 
StyI C'CwwG_G

Cuts at:      0    1451    1709    2127 
   Size:       1451     258     418

  Fragments arranged by size: 

                1451     418     258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     925    1627    1859    2127 
   Size:        925     702     232     268

  Fragments arranged by size: 

                 925     702     268     232
 
TatI w'GTAC_w

Cuts at:      0     411    1698    1802    2127 
   Size:        411    1287     104     325

  Fragments arranged by size: 

                1287     411     325     104
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      89     121     232     264     611     643    2127 
   Size:         89      32     111      32     347      32    1484

  Fragments arranged by size: 

                1484     347     111      89      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318    2127 
   Size:        318    1809
 
XhoI C'TCGA_G

Cuts at:      0    1020    2127 
   Size:       1020    1107
 
XmaI C'CCGG_G

Cuts at:      0      33    2102    2127 
   Size:         33    2069      25

  Fragments arranged by size: 

                2069      33      25
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AhdI     AleI     AlfI    AlwNI
     ApaI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     NsiI     NspI
     PasI    PflMI    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII
     SacI     ScaI    SexAI     SfcI     SmaI     SmlI     SphI     SspI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AloI    ApaLI
     AscI    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI    BglII
     BmtI     BplI   Bpu10I    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I     ClaI     DraI     DrdI     EagI     EciI     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PvuI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI
     SwaI  Tth111I     XbaI     XmnI     ZraI
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